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The map label for this gene is tgt [H]

Identifier: 16763785

GI number: 16763785

Start: 460821

End: 461948

Strand: Direct

Name: tgt [H]

Synonym: STM0405

Alternate gene names: 16763785

Gene position: 460821-461948 (Clockwise)

Preceding gene: 16763784

Following gene: 16763786

Centisome position: 9.49

GC content: 52.48

Gene sequence:

>1128_bases
ATGAAATTTGAGTTAGATACCACCGATGGTCGTGCGCGTCGCGGTCGTCTGGTGTTTGATCGTGGCGTAGTGGAAACGCC
AGCCTTTATGCCTGTGGGTACTTACGGTACCGTGAAAGGCATGACGCCGGAAGAAGTGGAAGCGACCGGCGCGCAGATTA
TTCTCGGCAACACTTTCCACCTGTGGCTGCGCCCGGGTCAGGAAATCATGAAGCTGCATGGCGATCTGCACGATTTTATG
CAGTGGAAAGGGCCGATTCTCACCGATTCCGGCGGCTTCCAGGTCTTCAGCCTCGGCGATATTCGTAAGATTACCGAGCA
AGGCGTGCACTTCCGTAACCCGATCAACGGCGATCCGATTTTCCTCGATCCGGAAAAGTCGATGGAGATCCAGTACGATC
TCGGTTCCGATATCGTGATGATCTTTGATGAATGTACGCCGTACCCGGCAGACTGGGACTATGCTAAACGCTCGATGGAG
ATGTCGTTGCGTTGGGCGAAGCGTAGCCGCGACCGTTTTGATAGCCTTGGCAATAAGAATGCGCTTTTTGGCATCATTCA
GGGCAGCGTTTACGAAGATTTACGCGATATCTCGGTGAAAGGTCTGGTAGAGATTGGCTTTGATGGCTACGCTGTCGGCG
GTTTGGCTGTCGGTGAGCCGAAAGCAGATATGCACCGCATTCTGGAGCACGTTTGCCCGCAGATCCCGGCTGACAAACCG
CGTTACCTGATGGGCGTGGGTAAACCGGAAGATCTGGTGGAAGGGGTGCGTCGCGGTATTGATATGTTTGACTGTGTCAT
GCCAACGCGAAATGCCCGTAATGGTCATCTGTTTGTGACCGACGGCGTGGTGAAGATTCGTAATGCAAAGCATAAAAGCG
ACACCAGCCCGCTTGACGCCGAGTGCGATTGCTACACCTGTCGCAATTATTCACGCGCTTACTTGCATCATCTCGACCGT
TGCAACGAAATATTAGGTGCGCGTCTCAATACCATTCATAACTTGCGTTACTACCAGCGTTTGATGGCGGGTTTACGCAA
GGCTATTGAAGAGGGTAAATTAGAGAGCTTCGTAACCGAGTTTTACCAACGTCAGGGGCGACCCGTTCCACCTTTGAACG
TTGATTAA

Upstream 100 bases:

>100_bases
ACTGGCTGCGCCGATGACGTCTTCGGGAATTTATTAGCTATGGAATCATTCCGTAGCGAGTCGTTAAACGTCGGACTGTT
TTTCTGACGCAGGAGTGATA

Downstream 100 bases:

>100_bases
TTTTAATAATGAGGGAATTTGAATGAGCTTTTTTATTTCTGATGCGGTAGCGGCAACAGGTGCTCCAGCGCAGGGCAGCC
CGATGTCTCTGATTTTAATG

Product: queuine tRNA-ribosyltransferase

Products: NA

Alternate protein names: Guanine insertion enzyme; tRNA-guanine transglycosylase [H]

Number of amino acids: Translated: 375; Mature: 375

Protein sequence:

>375_residues
MKFELDTTDGRARRGRLVFDRGVVETPAFMPVGTYGTVKGMTPEEVEATGAQIILGNTFHLWLRPGQEIMKLHGDLHDFM
QWKGPILTDSGGFQVFSLGDIRKITEQGVHFRNPINGDPIFLDPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSME
MSLRWAKRSRDRFDSLGNKNALFGIIQGSVYEDLRDISVKGLVEIGFDGYAVGGLAVGEPKADMHRILEHVCPQIPADKP
RYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGHLFVTDGVVKIRNAKHKSDTSPLDAECDCYTCRNYSRAYLHHLDR
CNEILGARLNTIHNLRYYQRLMAGLRKAIEEGKLESFVTEFYQRQGRPVPPLNVD

Sequences:

>Translated_375_residues
MKFELDTTDGRARRGRLVFDRGVVETPAFMPVGTYGTVKGMTPEEVEATGAQIILGNTFHLWLRPGQEIMKLHGDLHDFM
QWKGPILTDSGGFQVFSLGDIRKITEQGVHFRNPINGDPIFLDPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSME
MSLRWAKRSRDRFDSLGNKNALFGIIQGSVYEDLRDISVKGLVEIGFDGYAVGGLAVGEPKADMHRILEHVCPQIPADKP
RYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGHLFVTDGVVKIRNAKHKSDTSPLDAECDCYTCRNYSRAYLHHLDR
CNEILGARLNTIHNLRYYQRLMAGLRKAIEEGKLESFVTEFYQRQGRPVPPLNVD
>Mature_375_residues
MKFELDTTDGRARRGRLVFDRGVVETPAFMPVGTYGTVKGMTPEEVEATGAQIILGNTFHLWLRPGQEIMKLHGDLHDFM
QWKGPILTDSGGFQVFSLGDIRKITEQGVHFRNPINGDPIFLDPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSME
MSLRWAKRSRDRFDSLGNKNALFGIIQGSVYEDLRDISVKGLVEIGFDGYAVGGLAVGEPKADMHRILEHVCPQIPADKP
RYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGHLFVTDGVVKIRNAKHKSDTSPLDAECDCYTCRNYSRAYLHHLDR
CNEILGARLNTIHNLRYYQRLMAGLRKAIEEGKLESFVTEFYQRQGRPVPPLNVD

Specific function: Exchanges the guanine residue with 7-aminomethyl-7- deazaguanine in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hyper

COG id: COG0343

COG function: function code J; Queuine/archaeosine tRNA-ribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queuine tRNA-ribosyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI13654276, Length=361, Percent_Identity=42.382271468144, Blast_Score=298, Evalue=5e-81,
Organism=Escherichia coli, GI1786607, Length=375, Percent_Identity=97.8666666666667, Blast_Score=763, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17544680, Length=362, Percent_Identity=41.1602209944751, Blast_Score=284, Evalue=6e-77,
Organism=Caenorhabditis elegans, GI25146502, Length=140, Percent_Identity=31.4285714285714, Blast_Score=84, Evalue=9e-17,
Organism=Drosophila melanogaster, GI19920510, Length=371, Percent_Identity=43.6657681940701, Blast_Score=306, Evalue=1e-83,
Organism=Drosophila melanogaster, GI21355311, Length=264, Percent_Identity=28.4090909090909, Blast_Score=92, Evalue=5e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004803
- InterPro:   IPR002616 [H]

Pfam domain/function: PF01702 TGT [H]

EC number: =2.4.2.29 [H]

Molecular weight: Translated: 42522; Mature: 42522

Theoretical pI: Translated: 6.59; Mature: 6.59

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFELDTTDGRARRGRLVFDRGVVETPAFMPVGTYGTVKGMTPEEVEATGAQIILGNTFH
CEEECCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCEEEECCEEE
LWLRPGQEIMKLHGDLHDFMQWKGPILTDSGGFQVFSLGDIRKITEQGVHFRNPINGDPI
EEECCCHHHHHHHHHHHHHHHCCCCEEECCCCEEEEECCHHHHHHHCCCEECCCCCCCCE
FLDPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSMEMSLRWAKRSRDRFDSLGNKN
EECCCCCEEEEEECCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
ALFGIIQGSVYEDLRDISVKGLVEIGFDGYAVGGLAVGEPKADMHRILEHVCPQIPADKP
EEEEEECCHHHHHHHHCCHHEEEEECCCCEEECCEEECCCHHHHHHHHHHHCCCCCCCCC
RYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGHLFVTDGVVKIRNAKHKSDTSPLDA
CEEEECCCHHHHHHHHHHCCCHHHHHCCCCCCCCCEEEEECCEEEECCCCCCCCCCCCCC
ECDCYTCRNYSRAYLHHLDRCNEILGARLNTIHNLRYYQRLMAGLRKAIEEGKLESFVTE
CCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHH
FYQRQGRPVPPLNVD
HHHHCCCCCCCCCCC
>Mature Secondary Structure
MKFELDTTDGRARRGRLVFDRGVVETPAFMPVGTYGTVKGMTPEEVEATGAQIILGNTFH
CEEECCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCEEEECCEEE
LWLRPGQEIMKLHGDLHDFMQWKGPILTDSGGFQVFSLGDIRKITEQGVHFRNPINGDPI
EEECCCHHHHHHHHHHHHHHHCCCCEEECCCCEEEEECCHHHHHHHCCCEECCCCCCCCE
FLDPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSMEMSLRWAKRSRDRFDSLGNKN
EECCCCCEEEEEECCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
ALFGIIQGSVYEDLRDISVKGLVEIGFDGYAVGGLAVGEPKADMHRILEHVCPQIPADKP
EEEEEECCHHHHHHHHCCHHEEEEECCCCEEECCEEECCCHHHHHHHHHHHCCCCCCCCC
RYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGHLFVTDGVVKIRNAKHKSDTSPLDA
CEEEECCCHHHHHHHHHHCCCHHHHHCCCCCCCCCEEEEECCEEEECCCCCCCCCCCCCC
ECDCYTCRNYSRAYLHHLDRCNEILGARLNTIHNLRYYQRLMAGLRKAIEEGKLESFVTE
CCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHH
FYQRQGRPVPPLNVD
HHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA