Click here to switch to the map view.

The map label for this gene is pitA [H]

Identifier: 218930963

GI number: 218930963

Start: 4466766

End: 4468262

Strand: Direct

Name: pitA [H]

Synonym: YPO3967

Alternate gene names: 218930963

Gene position: 4466766-4468262 (Clockwise)

Preceding gene: 218930961

Following gene: 218930964

Centisome position: 95.98

GC content: 50.7

Gene sequence:

>1497_bases
ATGCTACATCTTTTCGCTGGCCTGGATTTCCATACCGGCCTTATGTTAGTTCTTGCTTTGTTGTTCGTGCTGTTTTACGA
AGCCATCAATGGTTTTCATGATACAGCCAATGCGGTTGCGACCGTAATCTATACCCGTGCCATGCGTTCACAGATCGCAG
TTGTGATGGCAGGGGTGTTTAACTTCCTCGGCGTGATGCTGGGTGGTTTGAGCGTGGCCTACGCCATTGTTCACTTACTG
CCTACTGATCTGCTGTTAAACGTCAGTTCCACGCATGGGTTGGCAATGGTCTTCTCCATGTTACTGGCCGCGATTATCTG
GAATTTGGGTACTTGGTATTTTGGTATTCCAGCCTCCAGTTCTCATACGCTGATTGGTGCTATCATCGGTATTGGTCTAA
CCAATGCATTAATGACCAGTACATCCGTTGTGGATGCGCTGAACGTTCCTAAGATGATACAAATCTTTCTGTCGCTGATC
TTATCCCCGATTGTGGGTCTGGTTATCGCCGGTTTGATGGTATTCCTGCTACGTCGTTACTGGAGCAGTACCAAAAAGCA
GCAACGTATTCACCTGACGCCTGCTGAACGTGAAAAGAAAGACGGTAAACGTAAACCGCCATTCTGGACCCGTACGGCGC
TGATCCTCTCCGCTATCGGTGTGAGTTTCTCTCACGGCGCTAACGATGGTCAGAAAGGGATTGGCTTGATCATGCTGGTA
TTAATCGGTGTGGCTCCGGCTGGTTTTGTCGTGAATATGAATGCGACCGGCTATGATATTGCCCGCACCCGCGATGCAGT
CACTCATGTGCAACTCTATTATCAGCAACATGCTGAGGCATTGCCTCATGCCATCGCGCTGAAACCACTGGTGCCAGCCC
CAGAAACCTTGCCAGGTACACCTGCGCAATTCCACTGTGACACTTCACGCGCGATGGTCGCCATTAACCATGCACAAGCG
CTGCTGACCGATCTGAAAAGCTATGATGACCTAACCGTTGATCAACGCAGCCAAATGCGTCGCTTGCTGATGTGTGTCGC
AGAAACCGCAGGAGCCGTTGCCAAACTGCCAGAAACCAGTGCCGAAAACCGCCGTTTCCTCAACAATCTGCGTACCGATT
TGTTGGATACCGTGGAATATGCACCAACCTGGATTATCGTAGCGGTTGCTCTGGCTCTGTCTCTCGGGACAATGGTCGGT
TGGAGACGCGTTGCCGTCACTATCGGTGAGAAGATCGGCAAAAAAGGCATGACCTATGCTCAAGGGGTTTCAGCCCAGAT
GACCGCGGCGGTCTCTATCGGTATTGCCAGTTATACCGGCATGCCAGTATCGACCACTCAAGTGCTCTCTTCTGCTGTTG
CCGGGACCATGTTGGTCGATGGCGGTGGCGTGCAGAGTAAAACGGTGAAGAGCATTATGTTGGCCTGGGTCTTAACCCTG
CCAATCTCTATCTTCCTTTCCGGTGCACTGTATTGGATAGCCTTAAAGTTCATTTAA

Upstream 100 bases:

>100_bases
TATGATGTTATGTCAAAAAAAGGTCATATTTTCCTTTTACCAACCCCCCATTGTCACTGATAATGCGCCGCGTTCATGTC
CACAAACTGGCGTAACGCTT

Downstream 100 bases:

>100_bases
GTTAATTCAACGCTAAGCTCATTCGACGGTAAGCCAATTCGACGAATTGTTATCACCGAGAGCCTTAGCGCGATGTCAGT
GATGAGCAATAAAAAGGCGG

Product: phosphate transport protein

Products: Proton [Cytoplasm]; phosphate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 498; Mature: 498

Protein sequence:

>498_residues
MLHLFAGLDFHTGLMLVLALLFVLFYEAINGFHDTANAVATVIYTRAMRSQIAVVMAGVFNFLGVMLGGLSVAYAIVHLL
PTDLLLNVSSTHGLAMVFSMLLAAIIWNLGTWYFGIPASSSHTLIGAIIGIGLTNALMTSTSVVDALNVPKMIQIFLSLI
LSPIVGLVIAGLMVFLLRRYWSSTKKQQRIHLTPAEREKKDGKRKPPFWTRTALILSAIGVSFSHGANDGQKGIGLIMLV
LIGVAPAGFVVNMNATGYDIARTRDAVTHVQLYYQQHAEALPHAIALKPLVPAPETLPGTPAQFHCDTSRAMVAINHAQA
LLTDLKSYDDLTVDQRSQMRRLLMCVAETAGAVAKLPETSAENRRFLNNLRTDLLDTVEYAPTWIIVAVALALSLGTMVG
WRRVAVTIGEKIGKKGMTYAQGVSAQMTAAVSIGIASYTGMPVSTTQVLSSAVAGTMLVDGGGVQSKTVKSIMLAWVLTL
PISIFLSGALYWIALKFI

Sequences:

>Translated_498_residues
MLHLFAGLDFHTGLMLVLALLFVLFYEAINGFHDTANAVATVIYTRAMRSQIAVVMAGVFNFLGVMLGGLSVAYAIVHLL
PTDLLLNVSSTHGLAMVFSMLLAAIIWNLGTWYFGIPASSSHTLIGAIIGIGLTNALMTSTSVVDALNVPKMIQIFLSLI
LSPIVGLVIAGLMVFLLRRYWSSTKKQQRIHLTPAEREKKDGKRKPPFWTRTALILSAIGVSFSHGANDGQKGIGLIMLV
LIGVAPAGFVVNMNATGYDIARTRDAVTHVQLYYQQHAEALPHAIALKPLVPAPETLPGTPAQFHCDTSRAMVAINHAQA
LLTDLKSYDDLTVDQRSQMRRLLMCVAETAGAVAKLPETSAENRRFLNNLRTDLLDTVEYAPTWIIVAVALALSLGTMVG
WRRVAVTIGEKIGKKGMTYAQGVSAQMTAAVSIGIASYTGMPVSTTQVLSSAVAGTMLVDGGGVQSKTVKSIMLAWVLTL
PISIFLSGALYWIALKFI
>Mature_498_residues
MLHLFAGLDFHTGLMLVLALLFVLFYEAINGFHDTANAVATVIYTRAMRSQIAVVMAGVFNFLGVMLGGLSVAYAIVHLL
PTDLLLNVSSTHGLAMVFSMLLAAIIWNLGTWYFGIPASSSHTLIGAIIGIGLTNALMTSTSVVDALNVPKMIQIFLSLI
LSPIVGLVIAGLMVFLLRRYWSSTKKQQRIHLTPAEREKKDGKRKPPFWTRTALILSAIGVSFSHGANDGQKGIGLIMLV
LIGVAPAGFVVNMNATGYDIARTRDAVTHVQLYYQQHAEALPHAIALKPLVPAPETLPGTPAQFHCDTSRAMVAINHAQA
LLTDLKSYDDLTVDQRSQMRRLLMCVAETAGAVAKLPETSAENRRFLNNLRTDLLDTVEYAPTWIIVAVALALSLGTMVG
WRRVAVTIGEKIGKKGMTYAQGVSAQMTAAVSIGIASYTGMPVSTTQVLSSAVAGTMLVDGGGVQSKTVKSIMLAWVLTL
PISIFLSGALYWIALKFI

Specific function: Low-affinity inorganic phosphate transport. Can also transport arsenate [H]

COG id: COG0306

COG function: function code P; Phosphate/sulphate permeases

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the inorganic phosphate transporter (PiT) (TC 2.A.20) family. Pit subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789907, Length=499, Percent_Identity=75.5511022044088, Blast_Score=758, Evalue=0.0,
Organism=Escherichia coli, GI1789360, Length=499, Percent_Identity=70.3406813627254, Blast_Score=687, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001204 [H]

Pfam domain/function: PF01384 PHO4 [H]

EC number: NA

Molecular weight: Translated: 53564; Mature: 53564

Theoretical pI: Translated: 9.91; Mature: 9.91

Prosite motif: PS00012 PHOSPHOPANTETHEINE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLHLFAGLDFHTGLMLVLALLFVLFYEAINGFHDTANAVATVIYTRAMRSQIAVVMAGVF
CEEEEECCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NFLGVMLGGLSVAYAIVHLLPTDLLLNVSSTHGLAMVFSMLLAAIIWNLGTWYFGIPASS
HHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCHHHHHHHHHHHHHHHCCCEEEECCCCC
SHTLIGAIIGIGLTNALMTSTSVVDALNVPKMIQIFLSLILSPIVGLVIAGLMVFLLRRY
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
WSSTKKQQRIHLTPAEREKKDGKRKPPFWTRTALILSAIGVSFSHGANDGQKGIGLIMLV
HHCCCCCCEEECCCCHHHHHCCCCCCCCHHHHHHHHHHHCCHHCCCCCCCCHHHHHHHHH
LIGVAPAGFVVNMNATGYDIARTRDAVTHVQLYYQQHAEALPHAIALKPLVPAPETLPGT
HHHCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCCCCCCCCCC
PAQFHCDTSRAMVAINHAQALLTDLKSYDDLTVDQRSQMRRLLMCVAETAGAVAKLPETS
CCEEEECCCCCEEEHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCC
AENRRFLNNLRTDLLDTVEYAPTWIIVAVALALSLGTMVGWRRVAVTIGEKIGKKGMTYA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHH
QGVSAQMTAAVSIGIASYTGMPVSTTQVLSSAVAGTMLVDGGGVQSKTVKSIMLAWVLTL
HCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHEEECCCCCCHHHHHHHHHHHHHHH
PISIFLSGALYWIALKFI
HHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MLHLFAGLDFHTGLMLVLALLFVLFYEAINGFHDTANAVATVIYTRAMRSQIAVVMAGVF
CEEEEECCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NFLGVMLGGLSVAYAIVHLLPTDLLLNVSSTHGLAMVFSMLLAAIIWNLGTWYFGIPASS
HHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCHHHHHHHHHHHHHHHCCCEEEECCCCC
SHTLIGAIIGIGLTNALMTSTSVVDALNVPKMIQIFLSLILSPIVGLVIAGLMVFLLRRY
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
WSSTKKQQRIHLTPAEREKKDGKRKPPFWTRTALILSAIGVSFSHGANDGQKGIGLIMLV
HHCCCCCCEEECCCCHHHHHCCCCCCCCHHHHHHHHHHHCCHHCCCCCCCCHHHHHHHHH
LIGVAPAGFVVNMNATGYDIARTRDAVTHVQLYYQQHAEALPHAIALKPLVPAPETLPGT
HHHCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCCCCCCCCCC
PAQFHCDTSRAMVAINHAQALLTDLKSYDDLTVDQRSQMRRLLMCVAETAGAVAKLPETS
CCEEEECCCCCEEEHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCC
AENRRFLNNLRTDLLDTVEYAPTWIIVAVALALSLGTMVGWRRVAVTIGEKIGKKGMTYA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHH
QGVSAQMTAAVSIGIASYTGMPVSTTQVLSSAVAGTMLVDGGGVQSKTVKSIMLAWVLTL
HCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHEEECCCCCCHHHHHHHHHHHHHHH
PISIFLSGALYWIALKFI
HHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; phosphate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + phosphate [Periplasm] = Proton [Cytoplasm] + phosphate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]