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The map label for this gene is glgX [H]

Identifier: 218930937

GI number: 218930937

Start: 4428398

End: 4430386

Strand: Reverse

Name: glgX [H]

Synonym: YPO3941

Alternate gene names: 218930937

Gene position: 4430386-4428398 (Counterclockwise)

Preceding gene: 218930938

Following gene: 218930936

Centisome position: 95.2

GC content: 53.7

Gene sequence:

>1989_bases
ATGGCGGTATTAACCCATGGATCACCGACACCTTCTGGCGCCCACTTTGATGGGAAGGGCATTAATTTCACACTATTTTC
AGCCCATGCTGAACAGGTGACGTTGTGTCTGTTTGATGAGCAGGGGCAAGAGCGGCAGATAGCCATGCCTGCTCGTACTG
GTGACATCTGGCATGGCTATCTTCCCGGCGGCAAGCCGGGGCAACGCTATGGTTACCGGGTCAGCGGGCCTTTCGAGCCC
AGTCGAGGCCACCGTTTTAACCCACATAAGTTGTTGATAGACCCCCGAACTCGTGCGTTAGAAGGCAAGGTTGGTGATGA
TCCCCGCTTTACCGGTGGCGTTAGCCAGCCCGATGTCCGCGACAGTGCGGCGGCGTTACCTAAATGTCTTGTGATACACG
AGGAATATGACTGGCAGGGGGATAAACCGCCCGCCATTCCTTGGGGCAATACCGTGATTTATGAAGCCCATGTCCGTGGT
TTAACCCAATTACATCCTGATATTCCGCCAGAGCTGCGCGGTACCTATGCCGCCTTGGCACACCCTGCTCTGATTGAACA
TCTCAAAACATTGGGGATTACCACACTGGAGTTGCTGCCAGTGCAATTTCATATTGATGAACCCCGGCTACAAAAAATGG
GGCTGAGCAATTACTGGGGCTATAACGTGTTAGCGCCCTTTGCGGTGGATCCAGATTATGCCTCGGGCCGCGAGGGCATA
TCACCGCTGAGAGAACTACGGGATGCGGTTAAAGCCCTGCATAACGCGGGCATTGAAGTCATTCTGGATGTGGTGTTCAA
CCACAGCGCTGAGTTGGATGTCTTCGGCCCGACCTTGTGTCAGCGTGGTATCGACAATGCCAGCTACTACTGGCTGACCC
CCGATGGTGAATACGACAACATCACGGGCTGCGGCAATGCACTGCGCCTCAGTCATCCGTATGTCACACAGTGGGTTATT
GATTGCCTAAATTATTGGCGCGATAGCTGCCATGTGGACGGCTTTCGTTTTGACTTAGGCACGGTACTGGGCCGCACACC
GGCGTTCGATCAACATGCGCCTTTATTTGCCGCCTTGGCGGCCGATGAGCGGCTCAGTGCCTGCAAATTGATTGCAGAAC
CTTGGGATATCGGTCTGGGCGGTTATCAATTGGGGAACTTCCCCACCGGCTTTAGCGAATGGAATGACCAGTACCGCGAT
GCCATGCGCGGTTTCTGGCTACGAGGGGAGGTGCCGCGCGGTACCTTTGCCCAGCATTTTGCTGCTTCAAGCCGCCTGTT
TGAACAGCGAGGGCGCTTACCCTCTGCCAGCATTAATCAAATTACCGCCCATGATGGTTTCACCTTGCTGGATTTGCTGT
GTTTTAACCAAAAACATAACCAAATGAATGGCGAAGAAAATCGTGATGGCAGTGATAACAACCATAGCAATAATTTTGGT
TGTGAAGGCTTGGTGGCTGACGCGGCTATCTGGCAACGGCGTAAAGCCTGTCAGCGGGCGCTACTGACCACCTTATTACT
CTCGCAGGGAACGCCGATGTTACTGGCCGGTGACGAACAGGGCCACAGCCAGCAAGGCAACAACAATGCTTATTGTCAGA
ACAACATCCTCACCTGGCTCGATTGGGGCAGTGCGGATCGTGCATTAATGACGTTTACCGCTGACCTTATCCGGCTGCGC
CAGCAAATCCCAGCCCTGACTCAGGACCAATGGTGGCAGAGCGGCGATAGTAATGTGCAATGGTTGGATAGTCAGGGACA
GGCGCTGAGTGATGCTGCTTGGGAGCAAGGCTGCCAGCAGCAACTACAGATTTTGTTATCTCAGCGCTGGTTGGTGCTCA
TCAATGCCACTGATCACGAGTGTGAAATGCACTTACCTGAAGGGGAATGGGAGGGGATTCCTCCCTTTGGGGTGTCAGAC
CATGCGGAGCGATTAACCACATGGCGCGGGTCCGCGCATACCATTTGTGTGCTCATAAAGAGAGATTAA

Upstream 100 bases:

>100_bases
GGCATTGATAGCCATCATGTGAGGAGCCACAACCACGAGCATTCGTTGTTACTCACCTTGCCACCGCTGGCAACGATTTA
CCTGCTGCGGGAGAACTAGG

Downstream 100 bases:

>100_bases
GGAGCTTTTATGGTGAGGTTTGAAAGTACAGACTCATTGATGTTGGCCAGACAATTACCCAATAAAACCGTGGCACTCAT
TCTGGCGGGAGGCCGTGGCT

Product: glycogen debranching enzyme

Products: NA

Alternate protein names: Glycogen operon protein GlgX [H]

Number of amino acids: Translated: 662; Mature: 661

Protein sequence:

>662_residues
MAVLTHGSPTPSGAHFDGKGINFTLFSAHAEQVTLCLFDEQGQERQIAMPARTGDIWHGYLPGGKPGQRYGYRVSGPFEP
SRGHRFNPHKLLIDPRTRALEGKVGDDPRFTGGVSQPDVRDSAAALPKCLVIHEEYDWQGDKPPAIPWGNTVIYEAHVRG
LTQLHPDIPPELRGTYAALAHPALIEHLKTLGITTLELLPVQFHIDEPRLQKMGLSNYWGYNVLAPFAVDPDYASGREGI
SPLRELRDAVKALHNAGIEVILDVVFNHSAELDVFGPTLCQRGIDNASYYWLTPDGEYDNITGCGNALRLSHPYVTQWVI
DCLNYWRDSCHVDGFRFDLGTVLGRTPAFDQHAPLFAALAADERLSACKLIAEPWDIGLGGYQLGNFPTGFSEWNDQYRD
AMRGFWLRGEVPRGTFAQHFAASSRLFEQRGRLPSASINQITAHDGFTLLDLLCFNQKHNQMNGEENRDGSDNNHSNNFG
CEGLVADAAIWQRRKACQRALLTTLLLSQGTPMLLAGDEQGHSQQGNNNAYCQNNILTWLDWGSADRALMTFTADLIRLR
QQIPALTQDQWWQSGDSNVQWLDSQGQALSDAAWEQGCQQQLQILLSQRWLVLINATDHECEMHLPEGEWEGIPPFGVSD
HAERLTTWRGSAHTICVLIKRD

Sequences:

>Translated_662_residues
MAVLTHGSPTPSGAHFDGKGINFTLFSAHAEQVTLCLFDEQGQERQIAMPARTGDIWHGYLPGGKPGQRYGYRVSGPFEP
SRGHRFNPHKLLIDPRTRALEGKVGDDPRFTGGVSQPDVRDSAAALPKCLVIHEEYDWQGDKPPAIPWGNTVIYEAHVRG
LTQLHPDIPPELRGTYAALAHPALIEHLKTLGITTLELLPVQFHIDEPRLQKMGLSNYWGYNVLAPFAVDPDYASGREGI
SPLRELRDAVKALHNAGIEVILDVVFNHSAELDVFGPTLCQRGIDNASYYWLTPDGEYDNITGCGNALRLSHPYVTQWVI
DCLNYWRDSCHVDGFRFDLGTVLGRTPAFDQHAPLFAALAADERLSACKLIAEPWDIGLGGYQLGNFPTGFSEWNDQYRD
AMRGFWLRGEVPRGTFAQHFAASSRLFEQRGRLPSASINQITAHDGFTLLDLLCFNQKHNQMNGEENRDGSDNNHSNNFG
CEGLVADAAIWQRRKACQRALLTTLLLSQGTPMLLAGDEQGHSQQGNNNAYCQNNILTWLDWGSADRALMTFTADLIRLR
QQIPALTQDQWWQSGDSNVQWLDSQGQALSDAAWEQGCQQQLQILLSQRWLVLINATDHECEMHLPEGEWEGIPPFGVSD
HAERLTTWRGSAHTICVLIKRD
>Mature_661_residues
AVLTHGSPTPSGAHFDGKGINFTLFSAHAEQVTLCLFDEQGQERQIAMPARTGDIWHGYLPGGKPGQRYGYRVSGPFEPS
RGHRFNPHKLLIDPRTRALEGKVGDDPRFTGGVSQPDVRDSAAALPKCLVIHEEYDWQGDKPPAIPWGNTVIYEAHVRGL
TQLHPDIPPELRGTYAALAHPALIEHLKTLGITTLELLPVQFHIDEPRLQKMGLSNYWGYNVLAPFAVDPDYASGREGIS
PLRELRDAVKALHNAGIEVILDVVFNHSAELDVFGPTLCQRGIDNASYYWLTPDGEYDNITGCGNALRLSHPYVTQWVID
CLNYWRDSCHVDGFRFDLGTVLGRTPAFDQHAPLFAALAADERLSACKLIAEPWDIGLGGYQLGNFPTGFSEWNDQYRDA
MRGFWLRGEVPRGTFAQHFAASSRLFEQRGRLPSASINQITAHDGFTLLDLLCFNQKHNQMNGEENRDGSDNNHSNNFGC
EGLVADAAIWQRRKACQRALLTTLLLSQGTPMLLAGDEQGHSQQGNNNAYCQNNILTWLDWGSADRALMTFTADLIRLRQ
QIPALTQDQWWQSGDSNVQWLDSQGQALSDAAWEQGCQQQLQILLSQRWLVLINATDHECEMHLPEGEWEGIPPFGVSDH
AERLTTWRGSAHTICVLIKRD

Specific function: Hydrolyzes the alpha-1,6-glucosidic linkages in glycogen which has first been partially depolymerized by phosphorylase. Shows only very little activity with native glycogen [H]

COG id: COG1523

COG function: function code G; Type II secretory pathway, pullulanase PulA and related glycosidases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family [H]

Homologues:

Organism=Escherichia coli, GI2367229, Length=658, Percent_Identity=60.790273556231, Blast_Score=828, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013780
- InterPro:   IPR006047
- InterPro:   IPR004193
- InterPro:   IPR017853
- InterPro:   IPR013781
- InterPro:   IPR022844
- InterPro:   IPR011837
- InterPro:   IPR013783
- InterPro:   IPR014756 [H]

Pfam domain/function: PF00128 Alpha-amylase; PF02922 CBM_48 [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 73913; Mature: 73781

Theoretical pI: Translated: 5.60; Mature: 5.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVLTHGSPTPSGAHFDGKGINFTLFSAHAEQVTLCLFDEQGQERQIAMPARTGDIWHGY
CEEEECCCCCCCCCCCCCCCCEEEEEECCCCEEEEEEECCCCCCCEEECCCCCCCCEECC
LPGGKPGQRYGYRVSGPFEPSRGHRFNPHKLLIDPRTRALEGKVGDDPRFTGGVSQPDVR
CCCCCCCCCCCEEECCCCCCCCCCCCCCCEEEECCCCCEECCCCCCCCCCCCCCCCCCCC
DSAAALPKCLVIHEEYDWQGDKPPAIPWGNTVIYEAHVRGLTQLHPDIPPELRGTYAALA
HHHHHCCEEEEEEECCCCCCCCCCCCCCCCEEEEEHHHCCHHHHCCCCCHHHCCHHHHHH
HPALIEHLKTLGITTLELLPVQFHIDEPRLQKMGLSNYWGYNVLAPFAVDPDYASGREGI
HHHHHHHHHHCCCEEEEEEEEEEECCCHHHHHCCCCCCCCCCEEECEECCCCCCCCCHHH
SPLRELRDAVKALHNAGIEVILDVVFNHSAELDVFGPTLCQRGIDNASYYWLTPDGEYDN
HHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCEEECCHHHHHHCCCCCEEEEECCCCCCCC
ITGCGNALRLSHPYVTQWVIDCLNYWRDSCHVDGFRFDLGTVLGRTPAFDQHAPLFAALA
CCCCCCEEEECCCHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHCCCCCCCCCCCHHEEHH
ADERLSACKLIAEPWDIGLGGYQLGNFPTGFSEWNDQYRDAMRGFWLRGEVPRGTFAQHF
HHHHHHHHHHHCCCCCCCCCCEECCCCCCCHHHHHHHHHHHHHCEEEECCCCCCHHHHHH
AASSRLFEQRGRLPSASINQITAHDGFTLLDLLCFNQKHNQMNGEENRDGSDNNHSNNFG
HHHHHHHHHHCCCCCCCCCEEECCCCHHHHHHHHHCCHHCCCCCCCCCCCCCCCCCCCCC
CEGLVADAAIWQRRKACQRALLTTLLLSQGTPMLLAGDEQGHSQQGNNNAYCQNNILTWL
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCEEECCCEEEE
DWGSADRALMTFTADLIRLRQQIPALTQDQWWQSGDSNVQWLDSQGQALSDAAWEQGCQQ
ECCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCCEEEECCCCCHHHHHHHHHHHHH
QLQILLSQRWLVLINATDHECEMHLPEGEWEGIPPFGVSDHAERLTTWRGSAHTICVLIK
HHHHHHCCCEEEEEECCCCCEEEECCCCCCCCCCCCCCCHHHHHHHHCCCCCCEEEEEEE
RD
CC
>Mature Secondary Structure 
AVLTHGSPTPSGAHFDGKGINFTLFSAHAEQVTLCLFDEQGQERQIAMPARTGDIWHGY
EEEECCCCCCCCCCCCCCCCEEEEEECCCCEEEEEEECCCCCCCEEECCCCCCCCEECC
LPGGKPGQRYGYRVSGPFEPSRGHRFNPHKLLIDPRTRALEGKVGDDPRFTGGVSQPDVR
CCCCCCCCCCCEEECCCCCCCCCCCCCCCEEEECCCCCEECCCCCCCCCCCCCCCCCCCC
DSAAALPKCLVIHEEYDWQGDKPPAIPWGNTVIYEAHVRGLTQLHPDIPPELRGTYAALA
HHHHHCCEEEEEEECCCCCCCCCCCCCCCCEEEEEHHHCCHHHHCCCCCHHHCCHHHHHH
HPALIEHLKTLGITTLELLPVQFHIDEPRLQKMGLSNYWGYNVLAPFAVDPDYASGREGI
HHHHHHHHHHCCCEEEEEEEEEEECCCHHHHHCCCCCCCCCCEEECEECCCCCCCCCHHH
SPLRELRDAVKALHNAGIEVILDVVFNHSAELDVFGPTLCQRGIDNASYYWLTPDGEYDN
HHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCEEECCHHHHHHCCCCCEEEEECCCCCCCC
ITGCGNALRLSHPYVTQWVIDCLNYWRDSCHVDGFRFDLGTVLGRTPAFDQHAPLFAALA
CCCCCCEEEECCCHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHCCCCCCCCCCCHHEEHH
ADERLSACKLIAEPWDIGLGGYQLGNFPTGFSEWNDQYRDAMRGFWLRGEVPRGTFAQHF
HHHHHHHHHHHCCCCCCCCCCEECCCCCCCHHHHHHHHHHHHHCEEEECCCCCCHHHHHH
AASSRLFEQRGRLPSASINQITAHDGFTLLDLLCFNQKHNQMNGEENRDGSDNNHSNNFG
HHHHHHHHHHCCCCCCCCCEEECCCCHHHHHHHHHCCHHCCCCCCCCCCCCCCCCCCCCC
CEGLVADAAIWQRRKACQRALLTTLLLSQGTPMLLAGDEQGHSQQGNNNAYCQNNILTWL
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCEEECCCEEEE
DWGSADRALMTFTADLIRLRQQIPALTQDQWWQSGDSNVQWLDSQGQALSDAAWEQGCQQ
ECCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCCEEEECCCCCHHHHHHHHHHHHH
QLQILLSQRWLVLINATDHECEMHLPEGEWEGIPPFGVSDHAERLTTWRGSAHTICVLIK
HHHHHHCCCEEEEEECCCCCEEEECCCCCCCCCCCCCCCHHHHHHHHCCCCCCEEEEEEE
RD
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Glycosylases; Glycosidases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA