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The map label for this gene is rffH [H]

Identifier: 218930864

GI number: 218930864

Start: 4334124

End: 4335005

Strand: Reverse

Name: rffH [H]

Synonym: YPO3861

Alternate gene names: 218930864

Gene position: 4335005-4334124 (Counterclockwise)

Preceding gene: 218930865

Following gene: 218930863

Centisome position: 93.15

GC content: 49.32

Gene sequence:

>882_bases
ATGAAAGGCATAATTCTGGCTGGTGGTTCAGGTTCCCGATTACACCCCATCACCCGTGGCGTCTCTAAGCAGTTGCTTCC
CATCTACGATAAACCCATGATCTATTACCCGCTGTCAGTATTGATGCTGGCAGGGATCCGTGACGTGTTGATTATCTCAA
CCCCGGAAGACTTACCATCGTTTCAGCGTTTGTTGGGCAACGGTGATGAGTTTGGTATTAATCTGAGTTATGCCGCGCAG
CCTAGCCCAGACGGGCTGGCACAGGCATTTATCATTGGTGAAGCGTTTATTGATAATGAGCCATGCTGTTTGGTGCTCGG
TGATAATATCTACTTCGGCCAAGGCTTTAGTCCAAAACTGAAAGCTGTCGCCGCGCGTCAGCAGGGCGCGACTGTTTTCG
GCTATCAGGTGATGGATCCAGAGCGCTTTGGCGTAGTGGAGTTTGATGACAACTTCCGCGCGTTGTCTATTGAAGAAAAA
CCGAGCCAGCCAAAATCAAATTGGGCGGTGACCGGGCTCTATTTCTACGATAACCAAGTGGTTGATTTTGCCAAGCAGGT
TAAACCTTCTGCTCGCGGTGAACTGGAAATTACCAGCATTAATCAAATGTATCTGGATCGCGGTGAGTTAACGGTTGAGC
TGCTAGGGCGCGGTTTTGCTTGGCTGGATACCGGCACCCATGACAGCCTGATCGAAGCCAGTACTTTTGTTCAAACGGTT
GAAAAACGCCAAGGTTTTAAAATTGCTTGTTTGGAAGAGATTGCCTGGCGTAACGGTTGGTTGGATGATGATGGCGTAAA
ACGTGCGGCAACCGCGCTGGCGAAAACCGGTTATGGCAAATATCTGTTGGATCTATTACATGCCCGTCCACGCCAGTATT
GA

Upstream 100 bases:

>100_bases
TGATGTTTTGCCTGTGTATAGCCTCTGCCTGATGGTCTCAGGAGATGGTATGTATCAGGAAATTGTATGTATCAGGAGAT
TGCATGTATCAGGAGAACGT

Downstream 100 bases:

>100_bases
GCCACTGGGCTGGGAAAGTGAATTTTTCCAGCGCCAAAGTGCAAAATTGATTTTTTCCGACTCAGCCCCGCCACTTAACC
CGGCTGAGTTGGCTGCTTTT

Product: glucose-1-phosphate thymidylyltransferase

Products: NA

Alternate protein names: G1P-TT 2; dTDP-glucose pyrophosphorylase 2; dTDP-glucose synthase 2 [H]

Number of amino acids: Translated: 293; Mature: 293

Protein sequence:

>293_residues
MKGIILAGGSGSRLHPITRGVSKQLLPIYDKPMIYYPLSVLMLAGIRDVLIISTPEDLPSFQRLLGNGDEFGINLSYAAQ
PSPDGLAQAFIIGEAFIDNEPCCLVLGDNIYFGQGFSPKLKAVAARQQGATVFGYQVMDPERFGVVEFDDNFRALSIEEK
PSQPKSNWAVTGLYFYDNQVVDFAKQVKPSARGELEITSINQMYLDRGELTVELLGRGFAWLDTGTHDSLIEASTFVQTV
EKRQGFKIACLEEIAWRNGWLDDDGVKRAATALAKTGYGKYLLDLLHARPRQY

Sequences:

>Translated_293_residues
MKGIILAGGSGSRLHPITRGVSKQLLPIYDKPMIYYPLSVLMLAGIRDVLIISTPEDLPSFQRLLGNGDEFGINLSYAAQ
PSPDGLAQAFIIGEAFIDNEPCCLVLGDNIYFGQGFSPKLKAVAARQQGATVFGYQVMDPERFGVVEFDDNFRALSIEEK
PSQPKSNWAVTGLYFYDNQVVDFAKQVKPSARGELEITSINQMYLDRGELTVELLGRGFAWLDTGTHDSLIEASTFVQTV
EKRQGFKIACLEEIAWRNGWLDDDGVKRAATALAKTGYGKYLLDLLHARPRQY
>Mature_293_residues
MKGIILAGGSGSRLHPITRGVSKQLLPIYDKPMIYYPLSVLMLAGIRDVLIISTPEDLPSFQRLLGNGDEFGINLSYAAQ
PSPDGLAQAFIIGEAFIDNEPCCLVLGDNIYFGQGFSPKLKAVAARQQGATVFGYQVMDPERFGVVEFDDNFRALSIEEK
PSQPKSNWAVTGLYFYDNQVVDFAKQVKPSARGELEITSINQMYLDRGELTVELLGRGFAWLDTGTHDSLIEASTFVQTV
EKRQGFKIACLEEIAWRNGWLDDDGVKRAATALAKTGYGKYLLDLLHARPRQY

Specific function: Catalyzes the formation of dTDP-glucose, from dTTP and glucose 1-phosphate, as well as its pyrophosphorolysis [H]

COG id: COG1209

COG function: function code M; dTDP-glucose pyrophosphorylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glucose-1-phosphate thymidylyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1790224, Length=293, Percent_Identity=86.6894197952219, Blast_Score=535, Evalue=1e-153,
Organism=Escherichia coli, GI1788351, Length=284, Percent_Identity=65.4929577464789, Blast_Score=400, Evalue=1e-113,
Organism=Caenorhabditis elegans, GI133931050, Length=183, Percent_Identity=25.1366120218579, Blast_Score=72, Evalue=5e-13,
Organism=Saccharomyces cerevisiae, GI6320148, Length=277, Percent_Identity=26.3537906137184, Blast_Score=82, Evalue=1e-16,
Organism=Drosophila melanogaster, GI21355443, Length=250, Percent_Identity=24.4, Blast_Score=75, Evalue=4e-14,
Organism=Drosophila melanogaster, GI24644084, Length=250, Percent_Identity=24.4, Blast_Score=75, Evalue=4e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005907
- InterPro:   IPR005835 [H]

Pfam domain/function: PF00483 NTP_transferase [H]

EC number: =2.7.7.24 [H]

Molecular weight: Translated: 32467; Mature: 32467

Theoretical pI: Translated: 5.02; Mature: 5.02

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKGIILAGGSGSRLHPITRGVSKQLLPIYDKPMIYYPLSVLMLAGIRDVLIISTPEDLPS
CCCEEEECCCCCCCCHHHHCCHHCCCCCCCCCEEEHHHHHHHHHCCCEEEEEECCCCCHH
FQRLLGNGDEFGINLSYAAQPSPDGLAQAFIIGEAFIDNEPCCLVLGDNIYFGQGFSPKL
HHHHHCCCCCEEEEEEEECCCCCCHHHHEEEEEHHHCCCCCEEEEECCEEEECCCCCHHH
KAVAARQQGATVFGYQVMDPERFGVVEFDDNFRALSIEEKPSQPKSNWAVTGLYFYDNQV
HHHHHHHCCCEEEEEEEECCCCCEEEEECCCEEEEEECCCCCCCCCCEEEEEEEEECCHH
VDFAKQVKPSARGELEITSINQMYLDRGELTVELLGRGFAWLDTGTHDSLIEASTFVQTV
HHHHHHCCCCCCCCEEEEEHHHHHHCCCCEEEEEECCCEEEEECCCCCHHHHHHHHHHHH
EKRQGFKIACLEEIAWRNGWLDDDGVKRAATALAKTGYGKYLLDLLHARPRQY
HHHCCCEEEHHHHHHHHCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHCCCCCC
>Mature Secondary Structure
MKGIILAGGSGSRLHPITRGVSKQLLPIYDKPMIYYPLSVLMLAGIRDVLIISTPEDLPS
CCCEEEECCCCCCCCHHHHCCHHCCCCCCCCCEEEHHHHHHHHHCCCEEEEEECCCCCHH
FQRLLGNGDEFGINLSYAAQPSPDGLAQAFIIGEAFIDNEPCCLVLGDNIYFGQGFSPKL
HHHHHCCCCCEEEEEEEECCCCCCHHHHEEEEEHHHCCCCCEEEEECCEEEECCCCCHHH
KAVAARQQGATVFGYQVMDPERFGVVEFDDNFRALSIEEKPSQPKSNWAVTGLYFYDNQV
HHHHHHHCCCEEEEEEEECCCCCEEEEECCCEEEEEECCCCCCCCCCEEEEEEEEECCHH
VDFAKQVKPSARGELEITSINQMYLDRGELTVELLGRGFAWLDTGTHDSLIEASTFVQTV
HHHHHHCCCCCCCCEEEEEHHHHHHCCCCEEEEEECCCEEEEECCCCCHHHHHHHHHHHH
EKRQGFKIACLEEIAWRNGWLDDDGVKRAATALAKTGYGKYLLDLLHARPRQY
HHHCCCEEEHHHHHHHHCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1379743; 9278503; 7559340 [H]