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The map label for this gene is livJ [H]

Identifier: 218930810

GI number: 218930810

Start: 4274244

End: 4275359

Strand: Reverse

Name: livJ [H]

Synonym: YPO3808

Alternate gene names: 218930810

Gene position: 4275359-4274244 (Counterclockwise)

Preceding gene: 218930812

Following gene: 218930809

Centisome position: 91.87

GC content: 50.09

Gene sequence:

>1116_bases
ATGAAATTAACAAAAGGTAAAGTGTTGCTGGCTGGGTGTATGGCAATGGCGATGAGTCATTCTGTGCTCGCGCAAGACAT
TAAGGTCGCTATTGTGGGGGCAATGTCCGGCCCGGTGGCCCAATACGGTGACATGCAATTTACCGGTGCGCGCCAGGCTA
TCGCCGATATCAACGCTAGCGGTGGTATTAAAGGCGATAAGCTGGTTGGTGTGGAATACGATGATGCCTGCGATCCGAAA
CAAGCCGTGGCTGTCGCGAACAAAGTAATAAACGATGGTATCCGTTATGTTATCGGCCATCTGTGCTCTTCTTCAACTCA
GCCAGCTTCAGATATTTATGAAGATGAAGGGGTGATAATGATCACCCCTGCTGCTACAAACGCGGATCTGACCACCCGTG
GTTACAAAATGATTATGCGCACCACCGGCCTGGATTCAGACCAGGGCCCTACTGCTGCCAAATATATTGTTGAGACGATT
AAACCTAAACGTATCGCTGTGGTACATGATAAACAGCAATACGGTGAAGGTCTGGCACGTTCCGTGCGCGACAGTCTGAA
AGAACAAGGTGCAGAGGTTGTGCTGTTTGAAGGTGTGACCGCAGGGGATAAAGATTTCTCGACTCTGGTTGCTCGCCTGA
AGAAAGAGAACGTCGATTTTGTTTATTTCGGCGGTTACTACCCAGAAATGGGCCAAATTCTGCGTCAGGCTAAGCAAGCA
GGCCTAACCGCCCGCTTTATGGGGCCAGAAGGTGTGGGTAACTCCTCACTCTCCAACATCGCGGGTGAGGCCTCTGAAGG
CATGCTGGTGACATTACCTAAGCGTTATGATCAGGTTCCGGCGAACCAACCTATTGTTGATGCGCTAAAAGCGAAAAAAC
TGGATCCAACAGGCCCATTTGTGTGGACCACTTATGCTGCGCTGCAATCACTGACGACGGCGATGGAACGTACTGGCAGC
AAAGAACCGGCTGATTTGGCTAACGATCTGAAAACCGGCAAACCGGTTGAGACCGTGATGGGGCCATTGAGCTGGGACGA
TAAAGGCGATTTGAAAGGGTTTGAGTTTGGTATTTTTGAATGGCATGCGGATGGTTCCTCTACCGCAGTCAAATAA

Upstream 100 bases:

>100_bases
GTTAAATCATTATTACTTATGAACAAAATGTTACATTAATGCTAAAACACTCAGACAAACACAACAAGCATGTTCACAAT
GACAGATGGGGAATTCGCGG

Downstream 100 bases:

>100_bases
CAAGAATAGTAGAACTATTGACAAGAATAGTAGAACTATCTCGTTTTAGGATTGCTCTCGCAGTAATCCGGCCCCTTCTA
TCAACTGCATCCCCTCGTAT

Product: branched-chain amino acid-binding protein

Products: ADP; phosphate; L-leucine [Cytoplasm] [C]

Alternate protein names: L-BP; LS-BP [H]

Number of amino acids: Translated: 371; Mature: 371

Protein sequence:

>371_residues
MKLTKGKVLLAGCMAMAMSHSVLAQDIKVAIVGAMSGPVAQYGDMQFTGARQAIADINASGGIKGDKLVGVEYDDACDPK
QAVAVANKVINDGIRYVIGHLCSSSTQPASDIYEDEGVIMITPAATNADLTTRGYKMIMRTTGLDSDQGPTAAKYIVETI
KPKRIAVVHDKQQYGEGLARSVRDSLKEQGAEVVLFEGVTAGDKDFSTLVARLKKENVDFVYFGGYYPEMGQILRQAKQA
GLTARFMGPEGVGNSSLSNIAGEASEGMLVTLPKRYDQVPANQPIVDALKAKKLDPTGPFVWTTYAALQSLTTAMERTGS
KEPADLANDLKTGKPVETVMGPLSWDDKGDLKGFEFGIFEWHADGSSTAVK

Sequences:

>Translated_371_residues
MKLTKGKVLLAGCMAMAMSHSVLAQDIKVAIVGAMSGPVAQYGDMQFTGARQAIADINASGGIKGDKLVGVEYDDACDPK
QAVAVANKVINDGIRYVIGHLCSSSTQPASDIYEDEGVIMITPAATNADLTTRGYKMIMRTTGLDSDQGPTAAKYIVETI
KPKRIAVVHDKQQYGEGLARSVRDSLKEQGAEVVLFEGVTAGDKDFSTLVARLKKENVDFVYFGGYYPEMGQILRQAKQA
GLTARFMGPEGVGNSSLSNIAGEASEGMLVTLPKRYDQVPANQPIVDALKAKKLDPTGPFVWTTYAALQSLTTAMERTGS
KEPADLANDLKTGKPVETVMGPLSWDDKGDLKGFEFGIFEWHADGSSTAVK
>Mature_371_residues
MKLTKGKVLLAGCMAMAMSHSVLAQDIKVAIVGAMSGPVAQYGDMQFTGARQAIADINASGGIKGDKLVGVEYDDACDPK
QAVAVANKVINDGIRYVIGHLCSSSTQPASDIYEDEGVIMITPAATNADLTTRGYKMIMRTTGLDSDQGPTAAKYIVETI
KPKRIAVVHDKQQYGEGLARSVRDSLKEQGAEVVLFEGVTAGDKDFSTLVARLKKENVDFVYFGGYYPEMGQILRQAKQA
GLTARFMGPEGVGNSSLSNIAGEASEGMLVTLPKRYDQVPANQPIVDALKAKKLDPTGPFVWTTYAALQSLTTAMERTGS
KEPADLANDLKTGKPVETVMGPLSWDDKGDLKGFEFGIFEWHADGSSTAVK

Specific function: This protein is a component of the leucine-specific transport system, which is one of the two periplasmic binding protein-dependent transport systems of the high-affinity transport of the branched-chain amino acids in E.coli [H]

COG id: COG0683

COG function: function code E; ABC-type branched-chain amino acid transport systems, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leucine-binding protein family [H]

Homologues:

Organism=Escherichia coli, GI1789867, Length=365, Percent_Identity=75.3424657534247, Blast_Score=596, Evalue=1e-172,
Organism=Escherichia coli, GI48994941, Length=367, Percent_Identity=74.6594005449591, Blast_Score=583, Evalue=1e-168,

Paralogues:

None

Copy number: 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001828
- InterPro:   IPR000709 [H]

Pfam domain/function: PF01094 ANF_receptor [H]

EC number: NA

Molecular weight: Translated: 39631; Mature: 39631

Theoretical pI: Translated: 5.50; Mature: 5.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLTKGKVLLAGCMAMAMSHSVLAQDIKVAIVGAMSGPVAQYGDMQFTGARQAIADINAS
CCCCCCHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCHHCCCEEECCHHHHHHHCCCC
GGIKGDKLVGVEYDDACDPKQAVAVANKVINDGIRYVIGHLCSSSTQPASDIYEDEGVIM
CCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCCEEE
ITPAATNADLTTRGYKMIMRTTGLDSDQGPTAAKYIVETIKPKRIAVVHDKQQYGEGLAR
EECCCCCCCCCHHHHHEEHHHCCCCCCCCCHHHHHHHHHCCCCEEEEEECHHHHHHHHHH
SVRDSLKEQGAEVVLFEGVTAGDKDFSTLVARLKKENVDFVYFGGYYPEMGQILRQAKQA
HHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHC
GLTARFMGPEGVGNSSLSNIAGEASEGMLVTLPKRYDQVPANQPIVDALKAKKLDPTGPF
CCEEEECCCCCCCCCHHHHHCCCCCCCEEEECCHHHCCCCCCCCHHHHHHHCCCCCCCCC
VWTTYAALQSLTTAMERTGSKEPADLANDLKTGKPVETVMGPLSWDDKGDLKGFEFGIFE
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCHHHHHCCCCCCCCCCCCCEEEEEEE
WHADGSSTAVK
EECCCCCCCCC
>Mature Secondary Structure
MKLTKGKVLLAGCMAMAMSHSVLAQDIKVAIVGAMSGPVAQYGDMQFTGARQAIADINAS
CCCCCCHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCHHCCCEEECCHHHHHHHCCCC
GGIKGDKLVGVEYDDACDPKQAVAVANKVINDGIRYVIGHLCSSSTQPASDIYEDEGVIM
CCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCCEEE
ITPAATNADLTTRGYKMIMRTTGLDSDQGPTAAKYIVETIKPKRIAVVHDKQQYGEGLAR
EECCCCCCCCCHHHHHEEHHHCCCCCCCCCHHHHHHHHHCCCCEEEEEECHHHHHHHHHH
SVRDSLKEQGAEVVLFEGVTAGDKDFSTLVARLKKENVDFVYFGGYYPEMGQILRQAKQA
HHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHC
GLTARFMGPEGVGNSSLSNIAGEASEGMLVTLPKRYDQVPANQPIVDALKAKKLDPTGPF
CCEEEECCCCCCCCCHHHHHCCCCCCCEEEECCHHHCCCCCCCCHHHHHHHCCCCCCCCC
VWTTYAALQSLTTAMERTGSKEPADLANDLKTGKPVETVMGPLSWDDKGDLKGFEFGIFE
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCHHHHHCCCCCCCCCCCCCEEEEEEE
WHADGSSTAVK
EECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-leucine [Periplasm]; H2O [C]

Specific reaction: ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3891753; 4077929; 2195019; 8041620; 9278503; 6990419; 9298646; 2649683 [H]