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The map label for this gene is mpl [H]

Identifier: 218930533

GI number: 218930533

Start: 3927599

End: 3928981

Strand: Reverse

Name: mpl [H]

Synonym: YPO3519

Alternate gene names: 218930533

Gene position: 3928981-3927599 (Counterclockwise)

Preceding gene: 218930536

Following gene: 218930530

Centisome position: 84.43

GC content: 50.04

Gene sequence:

>1383_bases
ATGCGCATTCACATCTTAGGTATCTGCGGCACATTTATGGGGGGCTTGGCGATGCTCGCCCGATCACTGGGACATGAAGT
GACCGGAGCGGATGCTAACGTGTATCCACCAATGAGTACTTTGCTTGAGAATCAAGGCATTGACTTAATTCAAGGCTATG
ATCCTGCCCAACTGAATCCAATACCGGACCTGGTGATTATTGGTAATGCGATGACTCGCGGTAACCCGTGTGTAGAAGCA
GTATTGGAGCAAGGCATTCCATATGTGTCAGGCCCACAGTGGTTGCATGACCATGTGCTCCCCGAGCGCTGGGTGCTGGC
GGTCGCGGGGACTCATGGTAAGACGACAACCGCTGGCATGCTGGCTTGGATCCTCGAAGCCTGCGGTTATGAACCCGGTT
TCGTGATTGGTGGGGTACCGGGTAATTTCGATGTCTCTGCCCGCTTAGGTAATAGCCCATTTTTTGTGATTGAAGCGGAT
GAATATGATTGTGCCTTCTTTGATAAGCGCTCTAAATTCGTTCATTACAGCCCACGCACCCTGATCATGAATAACCTTGA
GTTCGATCATGCTGATATTTTTGATGATATTAAGGCGATTCAAAAGCAATTCCATCATCTGGTCCGTTTGGTGCCAGGGA
AGGGTAAGATTATTTTGCCGGATAATGATAATCACCTGAAACAAGTGATGGCGATGGGGTGCTGGAGTGAGCAAGAGCTG
GTGGGCGAAACCGGCAGTTGGTTTGCCCGCAAAGTCTCTACCGATGCCAGTGTTTATGAAGTTTTACTCGATAATAATGT
GGTCGGCGAAGTGAACTGGTCATTGGTGGGTGAGCACAATATGCATAATGGCCTGATGGCGATTGCGGCCGCTCGCCACG
TTGGCGTGCTACCGGCTGATGCTTGCCGGGCATTAGGTGATTTTATTAATGCCTCCCGCCGGTTGGAACTACGCGGAGAA
GCGCATGGTGTCACGGTTTATGATGATTTTGCTCACCATCCTACCGCTATTCTTGCCACGCTGGCGGCCCTGCGCAGTAA
AGTCGGTGGAACAGCACGGATTCTGGCCGTACTGGAGCCACGCTCTAATACGATGAAACTGGGGTTATGCAAAAATGAGC
TGGCACCTTCATTAGGTCGCGCGGATGAAGTATTTCTGTTCCAGCCGCAGCACATTCCTTGGCAAGTGGTTGAGGTAGCT
GAAGCCTGTATCCAGCCAGCGTATTGGAGTGCTGATATCGATCAGTTAGTTGATATGATTGTGAAAACAGCACAGCCTGG
CGACCATATTCTGGTGATGAGCAACGGTGGATTTGGCGGGATTCACGATAAATTGCTTAGTGCATTGGATAAAAAAGCAG
AACAAGCAACGGCACTGTTTTAG

Upstream 100 bases:

>100_bases
GCCCAAAGGTAAGATTGATTCATAGGCAATTCCATTTCTTCTCTTTGATTCTGAGCGGTAGAATGGCTACAAATTTTAAG
CTTATTAATACGGAAGATTT

Downstream 100 bases:

>100_bases
GCTGGATAAAATATCCCCTGCAAGTGGGGGGAGTGTGCCGGGAATAACGACGAAGGATACAAATCATCATCTTGAAAATA
TGCCCCATGTTAATGGGGCA

Product: UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-m e so-diaminopimelate ligase

Products: NA

Alternate protein names: Murein peptide ligase [H]

Number of amino acids: Translated: 460; Mature: 460

Protein sequence:

>460_residues
MRIHILGICGTFMGGLAMLARSLGHEVTGADANVYPPMSTLLENQGIDLIQGYDPAQLNPIPDLVIIGNAMTRGNPCVEA
VLEQGIPYVSGPQWLHDHVLPERWVLAVAGTHGKTTTAGMLAWILEACGYEPGFVIGGVPGNFDVSARLGNSPFFVIEAD
EYDCAFFDKRSKFVHYSPRTLIMNNLEFDHADIFDDIKAIQKQFHHLVRLVPGKGKIILPDNDNHLKQVMAMGCWSEQEL
VGETGSWFARKVSTDASVYEVLLDNNVVGEVNWSLVGEHNMHNGLMAIAAARHVGVLPADACRALGDFINASRRLELRGE
AHGVTVYDDFAHHPTAILATLAALRSKVGGTARILAVLEPRSNTMKLGLCKNELAPSLGRADEVFLFQPQHIPWQVVEVA
EACIQPAYWSADIDQLVDMIVKTAQPGDHILVMSNGGFGGIHDKLLSALDKKAEQATALF

Sequences:

>Translated_460_residues
MRIHILGICGTFMGGLAMLARSLGHEVTGADANVYPPMSTLLENQGIDLIQGYDPAQLNPIPDLVIIGNAMTRGNPCVEA
VLEQGIPYVSGPQWLHDHVLPERWVLAVAGTHGKTTTAGMLAWILEACGYEPGFVIGGVPGNFDVSARLGNSPFFVIEAD
EYDCAFFDKRSKFVHYSPRTLIMNNLEFDHADIFDDIKAIQKQFHHLVRLVPGKGKIILPDNDNHLKQVMAMGCWSEQEL
VGETGSWFARKVSTDASVYEVLLDNNVVGEVNWSLVGEHNMHNGLMAIAAARHVGVLPADACRALGDFINASRRLELRGE
AHGVTVYDDFAHHPTAILATLAALRSKVGGTARILAVLEPRSNTMKLGLCKNELAPSLGRADEVFLFQPQHIPWQVVEVA
EACIQPAYWSADIDQLVDMIVKTAQPGDHILVMSNGGFGGIHDKLLSALDKKAEQATALF
>Mature_460_residues
MRIHILGICGTFMGGLAMLARSLGHEVTGADANVYPPMSTLLENQGIDLIQGYDPAQLNPIPDLVIIGNAMTRGNPCVEA
VLEQGIPYVSGPQWLHDHVLPERWVLAVAGTHGKTTTAGMLAWILEACGYEPGFVIGGVPGNFDVSARLGNSPFFVIEAD
EYDCAFFDKRSKFVHYSPRTLIMNNLEFDHADIFDDIKAIQKQFHHLVRLVPGKGKIILPDNDNHLKQVMAMGCWSEQEL
VGETGSWFARKVSTDASVYEVLLDNNVVGEVNWSLVGEHNMHNGLMAIAAARHVGVLPADACRALGDFINASRRLELRGE
AHGVTVYDDFAHHPTAILATLAALRSKVGGTARILAVLEPRSNTMKLGLCKNELAPSLGRADEVFLFQPQHIPWQVVEVA
EACIQPAYWSADIDQLVDMIVKTAQPGDHILVMSNGGFGGIHDKLLSALDKKAEQATALF

Specific function: Reutilizes the intact tripeptide L-alanyl-gamma-D- glutamyl-meso-diaminopimelate by linking it to UDP-N-acetylmuramic acid [H]

COG id: COG0773

COG function: function code M; UDP-N-acetylmuramate-alanine ligase

Gene ontology:

Cell location: Secreted [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1790680, Length=456, Percent_Identity=84.2105263157895, Blast_Score=805, Evalue=0.0,
Organism=Escherichia coli, GI1786279, Length=293, Percent_Identity=27.9863481228669, Blast_Score=100, Evalue=2e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005757
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: 6.3.2.- [C]

Molecular weight: Translated: 50108; Mature: 50108

Theoretical pI: Translated: 5.62; Mature: 5.62

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIHILGICGTFMGGLAMLARSLGHEVTGADANVYPPMSTLLENQGIDLIQGYDPAQLNP
CEEEEEEHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHCCCCCEEECCCCCCCCC
IPDLVIIGNAMTRGNPCVEAVLEQGIPYVSGPQWLHDHVLPERWVLAVAGTHGKTTTAGM
CCCEEEECCCCCCCCHHHHHHHHCCCCCCCCCHHHHHHCCCCCEEEEEECCCCCCHHHHH
LAWILEACGYEPGFVIGGVPGNFDVSARLGNSPFFVIEADEYDCAFFDKRSKFVHYSPRT
HHHHHHHHCCCCCEEECCCCCCCEEEEEECCCCEEEEECCCCCEEEECCCCCEEEECCCE
LIMNNLEFDHADIFDDIKAIQKQFHHLVRLVPGKGKIILPDNDNHLKQVMAMGCWSEQEL
EEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCHHHHHHHHHCCCCCHHH
VGETGSWFARKVSTDASVYEVLLDNNVVGEVNWSLVGEHNMHNGLMAIAAARHVGVLPAD
HCCCCHHHHHHHCCCHHHHHHHHCCCEEEEEEEEEEECCCCCCHHHHHHHHHHCCCCCHH
ACRALGDFINASRRLELRGEAHGVTVYDDFAHHPTAILATLAALRSKVGGTARILAVLEP
HHHHHHHHHCCCCEEEECCCCCCEEEECCCCCCHHHHHHHHHHHHHHCCCCEEEEEEECC
RSNTMKLGLCKNELAPSLGRADEVFLFQPQHIPWQVVEVAEACIQPAYWSADIDQLVDMI
CCCCEEEEECHHHHCCCCCCCCEEEEECCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHH
VKTAQPGDHILVMSNGGFGGIHDKLLSALDKKAEQATALF
HHCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MRIHILGICGTFMGGLAMLARSLGHEVTGADANVYPPMSTLLENQGIDLIQGYDPAQLNP
CEEEEEEHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHCCCCCEEECCCCCCCCC
IPDLVIIGNAMTRGNPCVEAVLEQGIPYVSGPQWLHDHVLPERWVLAVAGTHGKTTTAGM
CCCEEEECCCCCCCCHHHHHHHHCCCCCCCCCHHHHHHCCCCCEEEEEECCCCCCHHHHH
LAWILEACGYEPGFVIGGVPGNFDVSARLGNSPFFVIEADEYDCAFFDKRSKFVHYSPRT
HHHHHHHHCCCCCEEECCCCCCCEEEEEECCCCEEEEECCCCCEEEECCCCCEEEECCCE
LIMNNLEFDHADIFDDIKAIQKQFHHLVRLVPGKGKIILPDNDNHLKQVMAMGCWSEQEL
EEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCHHHHHHHHHCCCCCHHH
VGETGSWFARKVSTDASVYEVLLDNNVVGEVNWSLVGEHNMHNGLMAIAAARHVGVLPAD
HCCCCHHHHHHHCCCHHHHHHHHCCCEEEEEEEEEEECCCCCCHHHHHHHHHHCCCCCHH
ACRALGDFINASRRLELRGEAHGVTVYDDFAHHPTAILATLAALRSKVGGTARILAVLEP
HHHHHHHHHCCCCEEEECCCCCCEEEECCCCCCHHHHHHHHHHHHHHCCCCEEEEEEECC
RSNTMKLGLCKNELAPSLGRADEVFLFQPQHIPWQVVEVAEACIQPAYWSADIDQLVDMI
CCCCEEEEECHHHHCCCCCCCCEEEEECCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHH
VKTAQPGDHILVMSNGGFGGIHDKLLSALDKKAEQATALF
HHCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7610040; 9278503; 2843822; 7984428; 8808921 [H]