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The map label for this gene is pnp [H]

Identifier: 218930504

GI number: 218930504

Start: 3898480

End: 3900597

Strand: Reverse

Name: pnp [H]

Synonym: YPO3490

Alternate gene names: 218930504

Gene position: 3900597-3898480 (Counterclockwise)

Preceding gene: 218930505

Following gene: 218930503

Centisome position: 83.82

GC content: 50.09

Gene sequence:

>2118_bases
TTGCTGACTCCGATTATTCGTAAATTCCAGTATGGTCAACATACCGTAACTATCGAAACTGGTATGATGGCTCGCCAGGC
CACTGCAGCCGTAATGGTAAGCATGGACGACACTGCAGTATTCGTGACTGTTGTTGGTCAAAAGAAAGCCAAACCAGGCC
AGAGTTTCTTCCCTCTGACGGTTAACTATCAGGAGCGTACTTACGCTGCTGGCCGTATCCCAGGTAGCTTCTTCCGTCGT
GAAGGCCGCCCAAGTGAAGGCGAAACACTGACTTCACGTCTGATCGACCGCCCAATTCGCCCACTGTTCCCAGACAGCTT
CCTGAACGAAGTTCAAGTGATTGCAACCGTCGTTTCGGTTAACCCACAAATTAACCCAGACATCGTGGCACTGATTGGTG
CATCTGCGGCACTGAGCTTGTCAGGCATTCCATTTAACGGCCCAATCGGTGCTGCTCGCGTTGGTTTTATCAACGACCAG
TATGTGCTGAACCCAACCACTGACGAGCTGAAAGAGAGCCGTTTGGATCTGGTTGTTGCCGGTACTGCGGGTGCAGTGCT
GATGGTTGAATCAGAAGCCGATATTCTGTCCGAAGAGCAAATGTTGGGCGCGGTCGTCTTCGGCCACGAACAACAGCAAG
TTGTGATTGAAAATATTAACGCTTTGGTTGCTGAAGCTGGCAAACCTAAGTGGGATTGGCAGGCAGAGCCTGTCAACGAA
GCACTGCATGCGCGTGTTGCTGAACTGGCCGAAGCGCGTCTGGGCGATGCATACCGTATCACTGAAAAACAAGAACGTTA
TACGCAAGTTGATGCTATCAAAGCTGACGTGACCGAAGCGCTATTAGCGCAGGACGACACATTAGATGCCGCTGAAATTC
AGGATATCCTGGCCAGCGTTGAGAAAAATGTTGTGCGTAGCCGTGTGCTGCGTGGTGAGCCACGTATCGATGGTCGCGAA
AAAGACATGATCCGTGGTTTGGATGTACGTACTGGCATCTTGCCACGTACTCATGGTTCTGCCTTGTTCACCCGCGGTGA
AACTCAGGCGTTGGTTACCGCTACGCTGGGTACGGCACGTGACGCACAAAACATTGATGAGTTGATGGGTGAGCGCACAG
ATTCATTCCTGTTACACTATAATTTCCCTCCGTATTGTGTAGGCGAAACAGGGATGGTTGGCTCACCTAAGCGTCGTGAG
ATTGGCCATGGCCGCCTTGCGAAACGTGGTGTTCTGGCTGTGATGCCAAGTGCGAGCGAGTTCCCTTACACTATTCGTGT
GGTTTCTGAAATCACCGAGTCTAATGGTTCTTCCTCAATGGCTTCTGTTTGTGGTGCTTCTCTGGCTCTGATGGATGCGG
GCGTGCCAATTAAAGCCGCTGTTGCTGGTATTGCAATGGGCCTGGTAAAAGAGGGTGATAACTTTGTCGTTCTGTCTGAC
ATTCTGGGTGACGAAGATCACTTGGGCGACATGGACTTTAAAGTAGCCGGTAGCCGTGACGGTGTGACCGCACTGCAAAT
GGACATTAAAATTGAAGGTATTACCCGCGAAATTATGCAGGTAGCTCTGAATCAGGCTAAGGGTGCACGTTTGCACATTC
TGGGCGTGATGGAACAGGCTATCAGCACACCACGTGGCGATATCTCTGAGTTTGCTCCACGTATCTACACCATGAAGATC
AACCCTGAGAAAATCAAGGATGTGATCGGTAAAGGTGGTTCGGTGATCCGTGCGCTGACTGATGAAACTGGCACCACGAT
TGAAATCGAAGATGATGGTACCATTAAGATTGCAGCAACTGATGGCGATAAAGCAAAACACGCTATCCGTCGTATCGAAG
AGATTACTGCTGAAATCGAAGTGGGCCGTATTTACGCAGGTAAAGTCACCCGTATCGTTGATTTCGGTGCATTTGTTGCT
ATCGGTGGCGGTAAAGAAGGTCTGGTACATATTTCTCAAATCGCTGATAAGCGTGTAGAGAAAGTGACAGACTACCTGCA
GATGGGTCAAGATGTGCCAGTTAAAGTAATGGAAGTTGATCGTCAGGGCCGTATCCGCCTGAGCATCAAAGAAGCCACTA
CTCCTGACGCAGAAGCACCAGAAGCAGCAGCAGAGTAA

Upstream 100 bases:

>100_bases
AGGATTGTCATTAGTCGCGAGGATGCAGTGTGAAGGTATCAGTAACAGGTGGATTAGATCGTCAATACGATCAGGCACCG
CAAAGCATAAGGGTACAATT

Downstream 100 bases:

>100_bases
TTAAGTCATTCCACAGCTCCTTGCTCATCGGTAAGGGGCTGTGTTATCTCAGGGGCGGGATGCTCTTGTGTTAAACAAAT
GGATGAAAGGATGTTTATCC

Product: polynucleotide phosphorylase/polyadenylase

Products: NA

Alternate protein names: Polynucleotide phosphorylase; PNPase [H]

Number of amino acids: Translated: 705; Mature: 705

Protein sequence:

>705_residues
MLTPIIRKFQYGQHTVTIETGMMARQATAAVMVSMDDTAVFVTVVGQKKAKPGQSFFPLTVNYQERTYAAGRIPGSFFRR
EGRPSEGETLTSRLIDRPIRPLFPDSFLNEVQVIATVVSVNPQINPDIVALIGASAALSLSGIPFNGPIGAARVGFINDQ
YVLNPTTDELKESRLDLVVAGTAGAVLMVESEADILSEEQMLGAVVFGHEQQQVVIENINALVAEAGKPKWDWQAEPVNE
ALHARVAELAEARLGDAYRITEKQERYTQVDAIKADVTEALLAQDDTLDAAEIQDILASVEKNVVRSRVLRGEPRIDGRE
KDMIRGLDVRTGILPRTHGSALFTRGETQALVTATLGTARDAQNIDELMGERTDSFLLHYNFPPYCVGETGMVGSPKRRE
IGHGRLAKRGVLAVMPSASEFPYTIRVVSEITESNGSSSMASVCGASLALMDAGVPIKAAVAGIAMGLVKEGDNFVVLSD
ILGDEDHLGDMDFKVAGSRDGVTALQMDIKIEGITREIMQVALNQAKGARLHILGVMEQAISTPRGDISEFAPRIYTMKI
NPEKIKDVIGKGGSVIRALTDETGTTIEIEDDGTIKIAATDGDKAKHAIRRIEEITAEIEVGRIYAGKVTRIVDFGAFVA
IGGGKEGLVHISQIADKRVEKVTDYLQMGQDVPVKVMEVDRQGRIRLSIKEATTPDAEAPEAAAE

Sequences:

>Translated_705_residues
MLTPIIRKFQYGQHTVTIETGMMARQATAAVMVSMDDTAVFVTVVGQKKAKPGQSFFPLTVNYQERTYAAGRIPGSFFRR
EGRPSEGETLTSRLIDRPIRPLFPDSFLNEVQVIATVVSVNPQINPDIVALIGASAALSLSGIPFNGPIGAARVGFINDQ
YVLNPTTDELKESRLDLVVAGTAGAVLMVESEADILSEEQMLGAVVFGHEQQQVVIENINALVAEAGKPKWDWQAEPVNE
ALHARVAELAEARLGDAYRITEKQERYTQVDAIKADVTEALLAQDDTLDAAEIQDILASVEKNVVRSRVLRGEPRIDGRE
KDMIRGLDVRTGILPRTHGSALFTRGETQALVTATLGTARDAQNIDELMGERTDSFLLHYNFPPYCVGETGMVGSPKRRE
IGHGRLAKRGVLAVMPSASEFPYTIRVVSEITESNGSSSMASVCGASLALMDAGVPIKAAVAGIAMGLVKEGDNFVVLSD
ILGDEDHLGDMDFKVAGSRDGVTALQMDIKIEGITREIMQVALNQAKGARLHILGVMEQAISTPRGDISEFAPRIYTMKI
NPEKIKDVIGKGGSVIRALTDETGTTIEIEDDGTIKIAATDGDKAKHAIRRIEEITAEIEVGRIYAGKVTRIVDFGAFVA
IGGGKEGLVHISQIADKRVEKVTDYLQMGQDVPVKVMEVDRQGRIRLSIKEATTPDAEAPEAAAE
>Mature_705_residues
MLTPIIRKFQYGQHTVTIETGMMARQATAAVMVSMDDTAVFVTVVGQKKAKPGQSFFPLTVNYQERTYAAGRIPGSFFRR
EGRPSEGETLTSRLIDRPIRPLFPDSFLNEVQVIATVVSVNPQINPDIVALIGASAALSLSGIPFNGPIGAARVGFINDQ
YVLNPTTDELKESRLDLVVAGTAGAVLMVESEADILSEEQMLGAVVFGHEQQQVVIENINALVAEAGKPKWDWQAEPVNE
ALHARVAELAEARLGDAYRITEKQERYTQVDAIKADVTEALLAQDDTLDAAEIQDILASVEKNVVRSRVLRGEPRIDGRE
KDMIRGLDVRTGILPRTHGSALFTRGETQALVTATLGTARDAQNIDELMGERTDSFLLHYNFPPYCVGETGMVGSPKRRE
IGHGRLAKRGVLAVMPSASEFPYTIRVVSEITESNGSSSMASVCGASLALMDAGVPIKAAVAGIAMGLVKEGDNFVVLSD
ILGDEDHLGDMDFKVAGSRDGVTALQMDIKIEGITREIMQVALNQAKGARLHILGVMEQAISTPRGDISEFAPRIYTMKI
NPEKIKDVIGKGGSVIRALTDETGTTIEIEDDGTIKIAATDGDKAKHAIRRIEEITAEIEVGRIYAGKVTRIVDFGAFVA
IGGGKEGLVHISQIADKRVEKVTDYLQMGQDVPVKVMEVDRQGRIRLSIKEATTPDAEAPEAAAE

Specific function: Involved in mRNA degradation. Hydrolyzes single-stranded polyribonucleotides processively in the 3'- to 5'-direction [H]

COG id: COG1185

COG function: function code J; Polyribonucleotide nucleotidyltransferase (polynucleotide phosphorylase)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Homo sapiens, GI188528628, Length=708, Percent_Identity=40.1129943502825, Blast_Score=454, Evalue=1e-127,
Organism=Escherichia coli, GI145693187, Length=693, Percent_Identity=89.3217893217893, Blast_Score=1278, Evalue=0.0,
Organism=Caenorhabditis elegans, GI115534063, Length=713, Percent_Identity=34.6423562412342, Blast_Score=351, Evalue=7e-97,
Organism=Caenorhabditis elegans, GI17535281, Length=75, Percent_Identity=49.3333333333333, Blast_Score=72, Evalue=1e-12,
Organism=Drosophila melanogaster, GI281362905, Length=720, Percent_Identity=37.0833333333333, Blast_Score=460, Evalue=1e-129,
Organism=Drosophila melanogaster, GI24651641, Length=720, Percent_Identity=37.0833333333333, Blast_Score=460, Evalue=1e-129,
Organism=Drosophila melanogaster, GI24651643, Length=720, Percent_Identity=37.0833333333333, Blast_Score=460, Evalue=1e-129,
Organism=Drosophila melanogaster, GI161079377, Length=666, Percent_Identity=36.6366366366366, Blast_Score=418, Evalue=1e-117,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1000 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 3328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR004087
- InterPro:   IPR009019
- InterPro:   IPR004088
- InterPro:   IPR018111
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR012162
- InterPro:   IPR015848
- InterPro:   IPR003029
- InterPro:   IPR020568
- InterPro:   IPR022967 [H]

Pfam domain/function: PF00013 KH_1; PF03726 PNPase; PF01138 RNase_PH; PF03725 RNase_PH_C; PF00575 S1 [H]

EC number: =2.7.7.8 [H]

Molecular weight: Translated: 76197; Mature: 76197

Theoretical pI: Translated: 4.84; Mature: 4.84

Prosite motif: PS50084 KH_TYPE_1 ; PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTPIIRKFQYGQHTVTIETGMMARQATAAVMVSMDDTAVFVTVVGQKKAKPGQSFFPLT
CCCHHHHHHHCCCEEEEEECCCHHHHCEEEEEEEECCCEEEEEEECCCCCCCCCCEEEEE
VNYQERTYAAGRIPGSFFRREGRPSEGETLTSRLIDRPIRPLFPDSFLNEVQVIATVVSV
EECCHHEEECCCCCHHHHHCCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHEEC
NPQINPDIVALIGASAALSLSGIPFNGPIGAARVGFINDQYVLNPTTDELKESRLDLVVA
CCCCCCCEEEEECCCCEEEECCCCCCCCCCCEEEEEECCCEEECCCHHHHHHCCCCEEEE
GTAGAVLMVESEADILSEEQMLGAVVFGHEQQQVVIENINALVAEAGKPKWDWQAEPVNE
CCCCEEEEEECCCHHHHHHHHHHHEEECCCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHH
ALHARVAELAEARLGDAYRITEKQERYTQVDAIKADVTEALLAQDDTLDAAEIQDILASV
HHHHHHHHHHHHHCCCCEEECCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
EKNVVRSRVLRGEPRIDGREKDMIRGLDVRTGILPRTHGSALFTRGETQALVTATLGTAR
HHHHHHHHHHCCCCCCCCCHHHHHCCCCCCCCCCCCCCCCEEEECCCCCEEEEEECCCCC
DAQNIDELMGERTDSFLLHYNFPPYCVGETGMVGSPKRREIGHGRLAKRGVLAVMPSASE
CHHHHHHHHCCCCCCEEEEECCCCEECCCCCCCCCCCHHCCCCCCHHHCCEEEECCCCCC
FPYTIRVVSEITESNGSSSMASVCGASLALMDAGVPIKAAVAGIAMGLVKEGDNFVVLSD
CCEEEEEHHHHHHCCCCHHHHHHHCCHHEEHCCCCCHHHHHHHHHHHHHCCCCCEEEEHH
ILGDEDHLGDMDFKVAGSRDGVTALQMDIKIEGITREIMQVALNQAKGARLHILGVMEQA
HCCCCCCCCCCCEEEECCCCCCEEEEEEEEEEHHHHHHHHHHHHHCCCCEEEEHHHHHHH
ISTPRGDISEFAPRIYTMKINPEKIKDVIGKGGSVIRALTDETGTTIEIEDDGTIKIAAT
HCCCCCCHHHHCCEEEEEEECHHHHHHHHCCCCCEEEEEECCCCCEEEECCCCEEEEEEC
DGDKAKHAIRRIEEITAEIEVGRIYAGKVTRIVDFGAFVAIGGGKEGLVHISQIADKRVE
CCHHHHHHHHHHHHHHHHEEECEEEECCEEEEEECCEEEEECCCCCCCEEHHHHHHHHHH
KVTDYLQMGQDVPVKVMEVDRQGRIRLSIKEATTPDAEAPEAAAE
HHHHHHHCCCCCCEEEEEECCCCCEEEEEECCCCCCCCCCCCCCC
>Mature Secondary Structure
MLTPIIRKFQYGQHTVTIETGMMARQATAAVMVSMDDTAVFVTVVGQKKAKPGQSFFPLT
CCCHHHHHHHCCCEEEEEECCCHHHHCEEEEEEEECCCEEEEEEECCCCCCCCCCEEEEE
VNYQERTYAAGRIPGSFFRREGRPSEGETLTSRLIDRPIRPLFPDSFLNEVQVIATVVSV
EECCHHEEECCCCCHHHHHCCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHEEC
NPQINPDIVALIGASAALSLSGIPFNGPIGAARVGFINDQYVLNPTTDELKESRLDLVVA
CCCCCCCEEEEECCCCEEEECCCCCCCCCCCEEEEEECCCEEECCCHHHHHHCCCCEEEE
GTAGAVLMVESEADILSEEQMLGAVVFGHEQQQVVIENINALVAEAGKPKWDWQAEPVNE
CCCCEEEEEECCCHHHHHHHHHHHEEECCCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHH
ALHARVAELAEARLGDAYRITEKQERYTQVDAIKADVTEALLAQDDTLDAAEIQDILASV
HHHHHHHHHHHHHCCCCEEECCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
EKNVVRSRVLRGEPRIDGREKDMIRGLDVRTGILPRTHGSALFTRGETQALVTATLGTAR
HHHHHHHHHHCCCCCCCCCHHHHHCCCCCCCCCCCCCCCCEEEECCCCCEEEEEECCCCC
DAQNIDELMGERTDSFLLHYNFPPYCVGETGMVGSPKRREIGHGRLAKRGVLAVMPSASE
CHHHHHHHHCCCCCCEEEEECCCCEECCCCCCCCCCCHHCCCCCCHHHCCEEEECCCCCC
FPYTIRVVSEITESNGSSSMASVCGASLALMDAGVPIKAAVAGIAMGLVKEGDNFVVLSD
CCEEEEEHHHHHHCCCCHHHHHHHCCHHEEHCCCCCHHHHHHHHHHHHHCCCCCEEEEHH
ILGDEDHLGDMDFKVAGSRDGVTALQMDIKIEGITREIMQVALNQAKGARLHILGVMEQA
HCCCCCCCCCCCEEEECCCCCCEEEEEEEEEEHHHHHHHHHHHHHCCCCEEEEHHHHHHH
ISTPRGDISEFAPRIYTMKINPEKIKDVIGKGGSVIRALTDETGTTIEIEDDGTIKIAAT
HCCCCCCHHHHCCEEEEEEECHHHHHHHHCCCCCEEEEEECCCCCEEEECCCCEEEEEEC
DGDKAKHAIRRIEEITAEIEVGRIYAGKVTRIVDFGAFVAIGGGKEGLVHISQIADKRVE
CCHHHHHHHHHHHHHHHHEEECEEEECCEEEEEECCEEEEECCCCCCCEEHHHHHHHHHH
KVTDYLQMGQDVPVKVMEVDRQGRIRLSIKEATTPDAEAPEAAAE
HHHHHHHCCCCCCEEEEEECCCCCEEEEEECCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA