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The map label for this gene is mepA [H]

Identifier: 218930497

GI number: 218930497

Start: 3891373

End: 3892533

Strand: Reverse

Name: mepA [H]

Synonym: YPO3483

Alternate gene names: 218930497

Gene position: 3892533-3891373 (Counterclockwise)

Preceding gene: 218930499

Following gene: 218930496

Centisome position: 83.64

GC content: 52.54

Gene sequence:

>1161_bases
ATGCACAGTAAAATTATTTTGGCCTGTTTGGTGTTCACGCTTGTCGCTTGTGATCAATCCTCTTCACCTTCAGCGACACC
CTCGCGACAAGAGGTGGGGGTGGTGACCTTAAAAACTCAACCGGTCACATTGAGTAGCGATTTATCAGGCCGAACGGTGG
CGGCAATGACGTCTGAAGTCAGGCCACAGGTCGATGGTATCATCAAGAAACGCTTGTTTACTGAAGGATCTGAAGTCACC
GCGGGACAAGTGCTCTATCAAATAGACCCTGCTAGTTATCAGGCCGCTTATGACACCGCAAAAGCCGCGCTACAAAATGT
TCAGGTCAGCGTAAAATCGGCCAAGCTGAAAGCCCAGCGCTATGCGGCATTAGCCAAAGAAAATGGTGTGTCGCAGCAAG
ATGCTGATGACGCCCAAACCAGCTATCAGCAAGCGCTGGCCAATGTGGCCGAAAAAACTGCTGCGCTGGAAACGGCGCGT
ATCAATTTGGCCTATACCCAAGTGCGGGCACCCATTTCAGGTCGCATCGGCATTTCCTCTGTGACACCAGGGGCGCTGGT
GACGGCTAATCAAACCACGGCATTAGCCACCATCCGTAACCTTGATCCCATTTATGTTGATTTAACCCAGTCCAGTGCGC
AGTTGCTTGCTTTACGCAAACAGCAGCAGGCAGGCAATGACACAGTAGCGAATGCGCCGGTTCAGCTAACGCTGGAAGAT
GGCTCGGTTTATGCCCACGAAGGTTCTCTGCAACTGACCGAGGTGGCCGTGGATGAAGCGACCGGTGCGGTGACCTTGCG
TGCGAAATTCCCTAACCCTGAACACCAATTATTGCCGGGCATGTTTGTTCGCGCTTCAGTACGGAACGGTGTAAATAACA
CCGCTATTTTGGCACCACAGCAAGGCATCACTCATGATGCGAAAGGGAATGCTACCGCGTTGGTGGTTAATCAACAGCAG
CAAGTTGAGCGGCGTGAAGTGGTAACCGAACGCACGATTGATAGCTACTGGTTGATTAGCCGTGGGCTGGCTGCAGGTGA
CCGCCTGATTGTTGAAGGAACCGAAAAGGTCAGTGTTGGCGATGACGTCAAACCGGTAGAGGTGAGCACTACGCTGCCCG
TTGTGGCCGAACCGACTACGCCATCAACTGGGGAGAAATAA

Upstream 100 bases:

>100_bases
TTTTTCGGAATCCTTACGTAATGCTGAAAAAATCAAACAGTCGATATCCGTACAATTACGAACAATGACTATCAACACAA
TGAATGTACGGTAAAATTTT

Downstream 100 bases:

>100_bases
TCGATGTTTGCCCGTTTCTTTATTTATCGCCCGGTTTTTGCCTGGGTCATTGCCATTGTTATTATGTTGGGCGGGGTGGT
TGCGCTGGAAACCTTGCCGA

Product: multidrug efflux protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 386; Mature: 386

Protein sequence:

>386_residues
MHSKIILACLVFTLVACDQSSSPSATPSRQEVGVVTLKTQPVTLSSDLSGRTVAAMTSEVRPQVDGIIKKRLFTEGSEVT
AGQVLYQIDPASYQAAYDTAKAALQNVQVSVKSAKLKAQRYAALAKENGVSQQDADDAQTSYQQALANVAEKTAALETAR
INLAYTQVRAPISGRIGISSVTPGALVTANQTTALATIRNLDPIYVDLTQSSAQLLALRKQQQAGNDTVANAPVQLTLED
GSVYAHEGSLQLTEVAVDEATGAVTLRAKFPNPEHQLLPGMFVRASVRNGVNNTAILAPQQGITHDAKGNATALVVNQQQ
QVERREVVTERTIDSYWLISRGLAAGDRLIVEGTEKVSVGDDVKPVEVSTTLPVVAEPTTPSTGEK

Sequences:

>Translated_386_residues
MHSKIILACLVFTLVACDQSSSPSATPSRQEVGVVTLKTQPVTLSSDLSGRTVAAMTSEVRPQVDGIIKKRLFTEGSEVT
AGQVLYQIDPASYQAAYDTAKAALQNVQVSVKSAKLKAQRYAALAKENGVSQQDADDAQTSYQQALANVAEKTAALETAR
INLAYTQVRAPISGRIGISSVTPGALVTANQTTALATIRNLDPIYVDLTQSSAQLLALRKQQQAGNDTVANAPVQLTLED
GSVYAHEGSLQLTEVAVDEATGAVTLRAKFPNPEHQLLPGMFVRASVRNGVNNTAILAPQQGITHDAKGNATALVVNQQQ
QVERREVVTERTIDSYWLISRGLAAGDRLIVEGTEKVSVGDDVKPVEVSTTLPVVAEPTTPSTGEK
>Mature_386_residues
MHSKIILACLVFTLVACDQSSSPSATPSRQEVGVVTLKTQPVTLSSDLSGRTVAAMTSEVRPQVDGIIKKRLFTEGSEVT
AGQVLYQIDPASYQAAYDTAKAALQNVQVSVKSAKLKAQRYAALAKENGVSQQDADDAQTSYQQALANVAEKTAALETAR
INLAYTQVRAPISGRIGISSVTPGALVTANQTTALATIRNLDPIYVDLTQSSAQLLALRKQQQAGNDTVANAPVQLTLED
GSVYAHEGSLQLTEVAVDEATGAVTLRAKFPNPEHQLLPGMFVRASVRNGVNNTAILAPQQGITHDAKGNATALVVNQQQ
QVERREVVTERTIDSYWLISRGLAAGDRLIVEGTEKVSVGDDVKPVEVSTTLPVVAEPTTPSTGEK

Specific function: The periplasmic linker protein component of an organic solvent and antibiotic efflux pump; confers resistance to toluene, hexane, p-xylene, ampicillin, penicillin G, erythromycin, novobiocin and tetracycline [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Lipid-anchor (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI1789665, Length=353, Percent_Identity=50.1416430594901, Blast_Score=327, Evalue=6e-91,
Organism=Escherichia coli, GI1786668, Length=369, Percent_Identity=48.5094850948509, Blast_Score=323, Evalue=1e-89,
Organism=Escherichia coli, GI1789929, Length=360, Percent_Identity=46.3888888888889, Blast_Score=304, Evalue=6e-84,
Organism=Escherichia coli, GI87082036, Length=326, Percent_Identity=28.8343558282209, Blast_Score=128, Evalue=7e-31,
Organism=Escherichia coli, GI87081796, Length=320, Percent_Identity=24.6875, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 40943; Mature: 40943

Theoretical pI: Translated: 5.78; Mature: 5.78

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHSKIILACLVFTLVACDQSSSPSATPSRQEVGVVTLKTQPVTLSSDLSGRTVAAMTSEV
CCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEECCEEECCCCCCCEEEEHHHHC
RPQVDGIIKKRLFTEGSEVTAGQVLYQIDPASYQAAYDTAKAALQNVQVSVKSAKLKAQR
CHHHHHHHHHHHHCCCCCEECCEEEEEECCCHHHHHHHHHHHHHHHHEEEEHHHHHHHHH
YAALAKENGVSQQDADDAQTSYQQALANVAEKTAALETARINLAYTQVRAPISGRIGISS
HHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEEEEECCCCCCCEECC
VTPGALVTANQTTALATIRNLDPIYVDLTQSSAQLLALRKQQQAGNDTVANAPVQLTLED
CCCCEEEECCCCEEEEEHHCCCEEEEEECCCHHHHHHHHHHHHCCCCCCCCCCEEEEEEC
GSVYAHEGSLQLTEVAVDEATGAVTLRAKFPNPEHQLLPGMFVRASVRNGVNNTAILAPQ
CEEEEECCCEEEEEEEEECCCCEEEEEEECCCCCHHHCCCHHEEHHHHCCCCCCEEECCC
QGITHDAKGNATALVVNQQQQVERREVVTERTIDSYWLISRGLAAGDRLIVEGTEKVSVG
CCCCCCCCCCEEEEEECCHHHHHHHHHHHHHCCCHHHHHHCCCCCCCEEEEECCCEEECC
DDVKPVEVSTTLPVVAEPTTPSTGEK
CCCCCEEEECCCCEEECCCCCCCCCC
>Mature Secondary Structure
MHSKIILACLVFTLVACDQSSSPSATPSRQEVGVVTLKTQPVTLSSDLSGRTVAAMTSEV
CCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEECCEEECCCCCCCEEEEHHHHC
RPQVDGIIKKRLFTEGSEVTAGQVLYQIDPASYQAAYDTAKAALQNVQVSVKSAKLKAQR
CHHHHHHHHHHHHCCCCCEECCEEEEEECCCHHHHHHHHHHHHHHHHEEEEHHHHHHHHH
YAALAKENGVSQQDADDAQTSYQQALANVAEKTAALETARINLAYTQVRAPISGRIGISS
HHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEEEEECCCCCCCEECC
VTPGALVTANQTTALATIRNLDPIYVDLTQSSAQLLALRKQQQAGNDTVANAPVQLTLED
CCCCEEEECCCCEEEEEHHCCCEEEEEECCCHHHHHHHHHHHHCCCCCCCCCCEEEEEEC
GSVYAHEGSLQLTEVAVDEATGAVTLRAKFPNPEHQLLPGMFVRASVRNGVNNTAILAPQ
CEEEEECCCEEEEEEEEECCCCEEEEEEECCCCCHHHCCCHHEEHHHHCCCCCCEEECCC
QGITHDAKGNATALVVNQQQQVERREVVTERTIDSYWLISRGLAAGDRLIVEGTEKVSVG
CCCCCCCCCCEEEEEECCHHHHHHHHHHHHHCCCHHHHHHCCCCCCCEEEEECCCEEECC
DDVKPVEVSTTLPVVAEPTTPSTGEK
CCCCCEEEECCCCEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9827328 [H]