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The map label for this gene is ibeB [H]

Identifier: 218930495

GI number: 218930495

Start: 3886836

End: 3888242

Strand: Reverse

Name: ibeB [H]

Synonym: YPO3481

Alternate gene names: 218930495

Gene position: 3888242-3886836 (Counterclockwise)

Preceding gene: 218930496

Following gene: 218930491

Centisome position: 83.55

GC content: 50.96

Gene sequence:

>1407_bases
ATGTTCAGTCGTATACTGATATTATCGTTACCCGCGCTGCTGGCCGGATGTATTTCTCTCGATCCGGACTATCAGCGCCC
AGTGGCACCGGTTCCGGCAACCTTGCCTTACTCAACCGCCAGTAGCAGCAAGGCGGCAGATATTCCGTGGCAGGAGACGA
TCATTGATCCTAAACTGCGCCAGGTGATTGCGTTGGCATTAGAGAGCAACCGTGACCTACGCCAAGCTATTGCCGATATC
GAAGCGGCCCGTGCGCAATATGGTGTGCAACGGGCCGCCCAGATACCAACGGTTAATGCAGGAGTCAATGGCAGCCGTGG
GCGCGGATTGTCCGATACCTCTGATGGCAACAACAATACCGCTATCAGCCAGAGTTATGGTGCCCAAGCCAGTGTTAGTG
CCTTTGAACTGGATTTATTTGGTAAAAAAAGCAGCTTGTCTCATGCTGAATTTGAAACCTATCTGGCAACAGAAGAAGCG
GCGAAAACCACCCGCATCACACTGATTGCTGATACTGCGACAGCTTGGGTAACACTGGCGGCTGACCAGAATCAATTATT
GCTGGCCGAAGAGACGTTAAAAAGTGCGGAGCAATCTTTAAAATTGGCACAACTGCGGCAGAAAAATGGGATAGCGTCAC
GTATTGATGTAGCGGCGATGGAAACGTTGTATCAATCAGCGCGGGCCGATGTTGCACAATACAAAACCACGGTAGCGCAG
GATAAAAATGCACTGGATTTGTTGGTGGGCCAACCCGTGGCAGCACATTTACTGCCAGTGACGGCCTCTACATTGCCGGA
GGTCATCAAAGCTGTACCCGTTGGGCTTTCCTCTGATGTGTTGTTGAACCGGCCTGATGTATTGGAAGCCGAGCATAAAC
TGAAATCGGCCAATGCCAATATTGGGGCCGCTCGGGCGGCATTCTTCCCCAGTGTGACCTTAACCGCCAGTGGGGGCGTC
AGCAGTTCGGCACTTTCAACATTGCTTAGTGAGGGGGCAGGGATTTGGTCATTAGCCCCGGCGATTACCTTGCCGATATT
TGATGGCGGGGCAAATCGGGCTGCACTGGAATATTCACAGGCACAGAAAACCGGCTACATTGCCGCATATGAGAAAGCGA
TTCAGACGGCGTTTAAGGAAGTCGCCGATGCACTGGCGCGTAAAGCGACCATTCAAGAACAACGGGCAGCACAGCAAGCT
TACGTGGCGGCGGCACAAGAAAGTGTTGAACTGGCACAAAAACGCTATAAACAGGGAATCGATACATACTTGAATATGTT
AGATGCTCAACGCACCTTATATGCCGCGCAAACTCGTCTGATTGCCGTGCAACAGATACAAACTAACAATTTGATTACGT
TGTATAAAGTTTTAGGTGGTGGGGTATCTGACGTCGCCAAAGAATAG

Upstream 100 bases:

>100_bases
ACAGTGTTGGCCGTCTTTTTTGTCCCGCTCTTTTTTGTCTTGATTCGTCGAGTGTTCAGCGGCAAGTCAAAAAATATCAC
TGAACATAAAGGAGTATAAA

Downstream 100 bases:

>100_bases
ACATTTTTTCGCGGGCATATTTTTAGTCTATTGGGTACATCGTAGTCTATAAAATGCCGCTGTGATTAAGCGTTAATAAA
CATGAATAACAGGGAGGCCA

Product: putative outer membrane efflux lipoprotein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 468; Mature: 468

Protein sequence:

>468_residues
MFSRILILSLPALLAGCISLDPDYQRPVAPVPATLPYSTASSSKAADIPWQETIIDPKLRQVIALALESNRDLRQAIADI
EAARAQYGVQRAAQIPTVNAGVNGSRGRGLSDTSDGNNNTAISQSYGAQASVSAFELDLFGKKSSLSHAEFETYLATEEA
AKTTRITLIADTATAWVTLAADQNQLLLAEETLKSAEQSLKLAQLRQKNGIASRIDVAAMETLYQSARADVAQYKTTVAQ
DKNALDLLVGQPVAAHLLPVTASTLPEVIKAVPVGLSSDVLLNRPDVLEAEHKLKSANANIGAARAAFFPSVTLTASGGV
SSSALSTLLSEGAGIWSLAPAITLPIFDGGANRAALEYSQAQKTGYIAAYEKAIQTAFKEVADALARKATIQEQRAAQQA
YVAAAQESVELAQKRYKQGIDTYLNMLDAQRTLYAAQTRLIAVQQIQTNNLITLYKVLGGGVSDVAKE

Sequences:

>Translated_468_residues
MFSRILILSLPALLAGCISLDPDYQRPVAPVPATLPYSTASSSKAADIPWQETIIDPKLRQVIALALESNRDLRQAIADI
EAARAQYGVQRAAQIPTVNAGVNGSRGRGLSDTSDGNNNTAISQSYGAQASVSAFELDLFGKKSSLSHAEFETYLATEEA
AKTTRITLIADTATAWVTLAADQNQLLLAEETLKSAEQSLKLAQLRQKNGIASRIDVAAMETLYQSARADVAQYKTTVAQ
DKNALDLLVGQPVAAHLLPVTASTLPEVIKAVPVGLSSDVLLNRPDVLEAEHKLKSANANIGAARAAFFPSVTLTASGGV
SSSALSTLLSEGAGIWSLAPAITLPIFDGGANRAALEYSQAQKTGYIAAYEKAIQTAFKEVADALARKATIQEQRAAQQA
YVAAAQESVELAQKRYKQGIDTYLNMLDAQRTLYAAQTRLIAVQQIQTNNLITLYKVLGGGVSDVAKE
>Mature_468_residues
MFSRILILSLPALLAGCISLDPDYQRPVAPVPATLPYSTASSSKAADIPWQETIIDPKLRQVIALALESNRDLRQAIADI
EAARAQYGVQRAAQIPTVNAGVNGSRGRGLSDTSDGNNNTAISQSYGAQASVSAFELDLFGKKSSLSHAEFETYLATEEA
AKTTRITLIADTATAWVTLAADQNQLLLAEETLKSAEQSLKLAQLRQKNGIASRIDVAAMETLYQSARADVAQYKTTVAQ
DKNALDLLVGQPVAAHLLPVTASTLPEVIKAVPVGLSSDVLLNRPDVLEAEHKLKSANANIGAARAAFFPSVTLTASGGV
SSSALSTLLSEGAGIWSLAPAITLPIFDGGANRAALEYSQAQKTGYIAAYEKAIQTAFKEVADALARKATIQEQRAAQQA
YVAAAQESVELAQKRYKQGIDTYLNMLDAQRTLYAAQTRLIAVQQIQTNNLITLYKVLGGGVSDVAKE

Specific function: The ability to export antibiotics and solvents is dramatically decreased in the presence of the proton conductor carbonyl cyanide m-chlorophenylhydrazone (CCCP), showing that an energized inner membrane is required for efflux. It is thought that the mexB

COG id: COG1538

COG function: function code MU; Outer membrane protein

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor. Note=Attached to the outer membrane by a lipid anchor and via the transmembrane beta-barrel. The membrane anchor is not necessary for antibiotic efflux. In one report (PubMed:10889211) the Cys-18- Gly mutant is reported

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the outer membrane factor (OMF) (TC 1.B.17) family [H]

Homologues:

Organism=Escherichia coli, GI1786785, Length=465, Percent_Identity=38.7096774193548, Blast_Score=306, Evalue=2e-84,
Organism=Escherichia coli, GI2367348, Length=475, Percent_Identity=24.6315789473684, Blast_Score=105, Evalue=9e-24,
Organism=Escherichia coli, GI87082199, Length=416, Percent_Identity=27.6442307692308, Blast_Score=70, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003423
- InterPro:   IPR010131 [H]

Pfam domain/function: PF02321 OEP [H]

EC number: NA

Molecular weight: Translated: 49701; Mature: 49701

Theoretical pI: Translated: 5.89; Mature: 5.89

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
0.9 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
0.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFSRILILSLPALLAGCISLDPDYQRPVAPVPATLPYSTASSSKAADIPWQETIIDPKLR
CCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCHHHH
QVIALALESNRDLRQAIADIEAARAQYGVQRAAQIPTVNAGVNGSRGRGLSDTSDGNNNT
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCCCCCCCCCCCCCCCCCC
AISQSYGAQASVSAFELDLFGKKSSLSHAEFETYLATEEAAKTTRITLIADTATAWVTLA
EEECCCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHCCEEEEEEEEECCEEEEEEE
ADQNQLLLAEETLKSAEQSLKLAQLRQKNGIASRIDVAAMETLYQSARADVAQYKTTVAQ
CCCCCEEEHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
DKNALDLLVGQPVAAHLLPVTASTLPEVIKAVPVGLSSDVLLNRPDVLEAEHKLKSANAN
CCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEECCCCHHHHHHHHHHCCCC
IGAARAAFFPSVTLTASGGVSSSALSTLLSEGAGIWSLAPAITLPIFDGGANRAALEYSQ
CCHHHHHHCCCEEEEECCCCCHHHHHHHHHCCCCCEEECCEEEEEEECCCCCCHHHHHHH
AQKTGYIAAYEKAIQTAFKEVADALARKATIQEQRAAQQAYVAAAQESVELAQKRYKQGI
HHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DTYLNMLDAQRTLYAAQTRLIAVQQIQTNNLITLYKVLGGGVSDVAKE
HHHHHHHHHHHHHHHHHHHHHEEEEECCCCCEEHHHHHCCCHHHHHCC
>Mature Secondary Structure
MFSRILILSLPALLAGCISLDPDYQRPVAPVPATLPYSTASSSKAADIPWQETIIDPKLR
CCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCHHHH
QVIALALESNRDLRQAIADIEAARAQYGVQRAAQIPTVNAGVNGSRGRGLSDTSDGNNNT
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCCCCCCCCCCCCCCCCCC
AISQSYGAQASVSAFELDLFGKKSSLSHAEFETYLATEEAAKTTRITLIADTATAWVTLA
EEECCCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHCCEEEEEEEEECCEEEEEEE
ADQNQLLLAEETLKSAEQSLKLAQLRQKNGIASRIDVAAMETLYQSARADVAQYKTTVAQ
CCCCCEEEHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
DKNALDLLVGQPVAAHLLPVTASTLPEVIKAVPVGLSSDVLLNRPDVLEAEHKLKSANAN
CCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEECCCCHHHHHHHHHHCCCC
IGAARAAFFPSVTLTASGGVSSSALSTLLSEGAGIWSLAPAITLPIFDGGANRAALEYSQ
CCHHHHHHCCCEEEEECCCCCHHHHHHHHHCCCCCEEECCEEEEEEECCCCCCHHHHHHH
AQKTGYIAAYEKAIQTAFKEVADALARKATIQEQRAAQQAYVAAAQESVELAQKRYKQGI
HHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DTYLNMLDAQRTLYAAQTRLIAVQQIQTNNLITLYKVLGGGVSDVAKE
HHHHHHHHHHHHHHHHHHHHHEEEEECCCCCEEHHHHHCCCHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8226684; 10889211; 10984043; 7968531; 8540696; 9401051; 9603892; 10952562 [H]