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The map label for this gene is pdxJ

Identifier: 218929985

GI number: 218929985

Start: 3274232

End: 3274963

Strand: Reverse

Name: pdxJ

Synonym: YPO2930

Alternate gene names: 218929985

Gene position: 3274963-3274232 (Counterclockwise)

Preceding gene: 218929986

Following gene: 218929984

Centisome position: 70.37

GC content: 49.59

Gene sequence:

>732_bases
ATGGCTGATTTGTTGCTGGGCGTTAATATTGATCACATCGCGACACTACGAAATGCACGTGGGACCATTTATCCCGATCC
GGTTCAGGCTGCTTTTATTGCTGAGCAAGCGGGCGCTGACGGCATTACTGTACATTTACGTGAAGATCGCCGTCATATTA
CGGATCGCGATGTGCGTATTCTGCGTCAGACCATCCAGACTCGCATGAATCTGGAAATGGCGGTAACGGATGAAATGGTT
GATATTGCTTGTGATATCAAACCGCATTTTTGTTGTCTGGTACCTGAAAAACGTCAGGAAGTCACGACTGAAGGCGGGCT
AGATGTTGCCGGTCAGGTTGATAAAATGACCCTGGCGGTAGGGCGGTTAGCGGATGTTGGCATACTGGTTTCACTCTTTA
TTGATGCAGATTTTCGCCAAATCGATGCGGCTGTTGCCGCTGGCGCACCTTATATTGAAATTCACACCGGTGCTTATGCT
GATGCCTCGACCGTTCTGGAACGTCAGGCTGAATTAATGCGTATCGCCAAAGCAGCGACCTATGCTGCAGGCAAAGGGTT
GAAAGTGAATGCGGGTCATGGTCTGACATACCATAATGTGCAGCCAATAGCTGCATTGCCAGAAATGCATGAGCTGAATA
TTGGTCATGCGATCATTGGCCAGGCTGTGATGACAGGCCTTGCTGCCGCAGTGACAGACATGAAGGTATTGATGCGGGAA
GCGCGCCGTTAA

Upstream 100 bases:

>100_bases
CTAGTCGGTGTAAACTGAAAATCACTGACGTGGTTATTGGTATGCTTTATTTATATGCTTATTAACCGATTAAAGATGCA
TCCATATTTGGAGGGCTGTC

Downstream 100 bases:

>100_bases
TGGCTATTCTTGGGCTAGGAACGGATATCGTTGAAATCTCACGTATTCAAGCGGTTGTTGAGCGTACTGGTGAGAGGCTG
GCGCGGCGTATTCTCAGCCC

Product: pyridoxine 5'-phosphate synthase

Products: NA

Alternate protein names: PNP synthase

Number of amino acids: Translated: 243; Mature: 242

Protein sequence:

>243_residues
MADLLLGVNIDHIATLRNARGTIYPDPVQAAFIAEQAGADGITVHLREDRRHITDRDVRILRQTIQTRMNLEMAVTDEMV
DIACDIKPHFCCLVPEKRQEVTTEGGLDVAGQVDKMTLAVGRLADVGILVSLFIDADFRQIDAAVAAGAPYIEIHTGAYA
DASTVLERQAELMRIAKAATYAAGKGLKVNAGHGLTYHNVQPIAALPEMHELNIGHAIIGQAVMTGLAAAVTDMKVLMRE
ARR

Sequences:

>Translated_243_residues
MADLLLGVNIDHIATLRNARGTIYPDPVQAAFIAEQAGADGITVHLREDRRHITDRDVRILRQTIQTRMNLEMAVTDEMV
DIACDIKPHFCCLVPEKRQEVTTEGGLDVAGQVDKMTLAVGRLADVGILVSLFIDADFRQIDAAVAAGAPYIEIHTGAYA
DASTVLERQAELMRIAKAATYAAGKGLKVNAGHGLTYHNVQPIAALPEMHELNIGHAIIGQAVMTGLAAAVTDMKVLMRE
ARR
>Mature_242_residues
ADLLLGVNIDHIATLRNARGTIYPDPVQAAFIAEQAGADGITVHLREDRRHITDRDVRILRQTIQTRMNLEMAVTDEMVD
IACDIKPHFCCLVPEKRQEVTTEGGLDVAGQVDKMTLAVGRLADVGILVSLFIDADFRQIDAAVAAGAPYIEIHTGAYAD
ASTVLERQAELMRIAKAATYAAGKGLKVNAGHGLTYHNVQPIAALPEMHELNIGHAIIGQAVMTGLAAAVTDMKVLMREA
RR

Specific function: Catalyzes the complicated ring closure reaction between the two acyclic compounds 1-deoxy-D-xylulose-5-phosphate (DXP) and 3-amino-2-oxopropyl phosphate (1-amino-acetone-3-phosphate or AAP) to form pyridoxine 5'-phosphate (PNP) and inorganic phosphate

COG id: COG0854

COG function: function code H; Pyridoxal phosphate biosynthesis protein

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PNP synthase family

Homologues:

Organism=Escherichia coli, GI1788917, Length=242, Percent_Identity=81.4049586776859, Blast_Score=391, Evalue=1e-110,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PDXJ_YERPE (Q8ZCP4)

Other databases:

- EMBL:   AL590842
- EMBL:   AE009952
- EMBL:   AE017042
- PIR:   AG0356
- RefSeq:   NP_668623.1
- RefSeq:   NP_993846.1
- RefSeq:   YP_002347860.1
- PDB:   3F4N
- PDBsum:   3F4N
- ProteinModelPortal:   Q8ZCP4
- SMR:   Q8ZCP4
- IntAct:   Q8ZCP4
- GeneID:   1146247
- GeneID:   1175751
- GeneID:   2766923
- GenomeReviews:   AE009952_GR
- GenomeReviews:   AE017042_GR
- GenomeReviews:   AL590842_GR
- KEGG:   ype:YPO2930
- KEGG:   ypk:y1300
- KEGG:   ypm:YP_2525
- HOGENOM:   HBG299314
- OMA:   AGHGLNY
- ProtClustDB:   PRK05265
- BioCyc:   YPES187410:Y1300-MONOMER
- BioCyc:   YPES214092:YPO2930-MONOMER
- BRENDA:   2.6.99.2
- GO:   GO:0005737
- HAMAP:   MF_00279
- InterPro:   IPR013785
- InterPro:   IPR004569
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00559

Pfam domain/function: PF03740 PdxJ; SSF63892 PyrdxlP_synth_PdxJ

EC number: =2.6.99.2

Molecular weight: Translated: 26298; Mature: 26167

Theoretical pI: Translated: 6.03; Mature: 6.03

Prosite motif: NA

Important sites: ACT_SITE 45-45 ACT_SITE 72-72 ACT_SITE 193-193 BINDING 9-9 BINDING 20-20 BINDING 47-47 BINDING 52-52 BINDING 102-102 BINDING 194-194

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADLLLGVNIDHIATLRNARGTIYPDPVQAAFIAEQAGADGITVHLREDRRHITDRDVRI
CCCEEECCCHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHH
LRQTIQTRMNLEMAVTDEMVDIACDIKPHFCCLVPEKRQEVTTEGGLDVAGQVDKMTLAV
HHHHHHHHHCCEEEEHHHHEEEEECCCCCEEEECCCHHHHHCCCCCCCCCCCHHHHHHHH
GRLADVGILVSLFIDADFRQIDAAVAAGAPYIEIHTGAYADASTVLERQAELMRIAKAAT
HHHHHHHHHHHHHHCCCHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHH
YAAGKGLKVNAGHGLTYHNVQPIAALPEMHELNIGHAIIGQAVMTGLAAAVTDMKVLMRE
HHCCCCEEEECCCCCEECCCCCHHHCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
ARR
HCC
>Mature Secondary Structure 
ADLLLGVNIDHIATLRNARGTIYPDPVQAAFIAEQAGADGITVHLREDRRHITDRDVRI
CCEEECCCHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHH
LRQTIQTRMNLEMAVTDEMVDIACDIKPHFCCLVPEKRQEVTTEGGLDVAGQVDKMTLAV
HHHHHHHHHCCEEEEHHHHEEEEECCCCCEEEECCCHHHHHCCCCCCCCCCCHHHHHHHH
GRLADVGILVSLFIDADFRQIDAAVAAGAPYIEIHTGAYADASTVLERQAELMRIAKAAT
HHHHHHHHHHHHHHCCCHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHH
YAAGKGLKVNAGHGLTYHNVQPIAALPEMHELNIGHAIIGQAVMTGLAAAVTDMKVLMRE
HHCCCCEEEECCCCCEECCCCCHHHCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
ARR
HCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11586360; 12142430