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The map label for this gene is purF [H]

Identifier: 218929844

GI number: 218929844

Start: 3106236

End: 3107753

Strand: Direct

Name: purF [H]

Synonym: YPO2772

Alternate gene names: 218929844

Gene position: 3106236-3107753 (Clockwise)

Preceding gene: 218929843

Following gene: 218929845

Centisome position: 66.75

GC content: 47.36

Gene sequence:

>1518_bases
ATGTGCGGTATTGTCGGTATCGCCGGTTTTACACCGGTAAACCAGTCGATTTATGATGCGTTGATGGTGCTTCAGCATCG
GGGTCAGGATGCGGCGGGTATTGTTACCATTGATGCCCATAATGGGTTCCGTCTGCGGAAAGCGAACGGCTTGGTGAAGG
ATGTATTTGAAACCCGGCATATGCTGCGTTTACAGGGGAATATGGGGATTGGTCATGTTCGTTACCCAACGGCAGGCAGC
TCCAGCGCTTCTGAAGCTCAACCTTTCTATGTTAACTCACCGTTTGGCATTACCCTGGCTCACAATGGTAATCTGACTAA
CGCTCACCAACTGAGAAAGAAATTATTCGAAGTTTCTCGTCGTCATGTAAATACCACGTCTGACTCAGAAATTCTGTTGA
ATATTTTTGCCAGTGAATTGGATCGTTTCCAGCATTATCCGTTGGAGTCTGACAACATTTTTGCCGCTGTTGCCGCGACG
CACCAGTTGATTCGTGGTGCTTATGCCTGCGTCGCAATGATTATCGGCCACGGTATGGTCGCTTTCCGTGACCCCAACGG
TATTCGTCCGCTGGTGATTGGTAAACGCACGCTGGCTGATGGGCGCAATGAGTATATGGTTGCGTCCGAAAGTGTGGCTT
TGGATACACTTGATTTTGAGTTCTTGCGTGATGTTGCACCGGGCGAAGCCGTGTATATCACCGAGAAAGGCCAACTGTTC
ACGCGTCAGTGTGCTGAGAATCCAAAATACAACCCATGCTTGTTTGAATATGTTTATTTTGCCCGCCCAGACTCCTTTAT
GGATAAAATTTCTGTTTACAGTGCGCGCGTCCGCATGGGGCAGAAGTTGGGGACCAAAATAGCCAAACAGTGGGAAGACA
TGGATATCGATGTTGTCATTCCTATTCCAGAAACCTCTTGCGATATCGCACTGGAAATTGCCCGAATTCTGGATAAACCG
TACCGTCAGGGGTTTGTCAAAAACCGTTATGTCGGGCGTACATTTATCATGCCGGGTCAGCAAGAACGCCGTAAATCGGT
GCGTCGTAAGCTGAATGCTAATCGTGCTGAGTTCCGTGGTAAAAACGTGTTATTGGTGGATGATTCCATTGTTCGCGGGA
CCACGTCAGAGCAGATTGTCGAAATGGCACGGGAAGCTGGGGCGAAAAAAGTGTACTTTGCGTCGGCAGCACCTGAAATC
CGTTTCCCGAATGTGTACGGTATCGATATGCCAAGTGCTAATGAGTTGATTGCTCATGGCCGTGAAGTTGATGAAATTCG
TCAGTTGATTGGCGCCGATGCACTGATTTTCCAAGATCTTACTGATCTTATTGATGCAGTACGTGAAGATAATCCAGACA
TTACCCAGTTCGAATGTTCTGTCTTTAATGGTATTTACGTGACGAAAGATGTGGATCAGAGCTATCTGGAATATCTTGAG
TCATTACGTAACGACGATGCGCAGGCATTGCGTAGCCACAATGAAGCGGAAAATTTAGAGATGCATAACGAAGGTTAA

Upstream 100 bases:

>100_bases
CAGTTATATCATCAGGTGGTTCTTTGACTACCTGCAGAGCACGTCGAGTTTCTTACCGAATCAATTACCGATTCGAAGCG
GCTTATGAGGAAAAGACAAC

Downstream 100 bases:

>100_bases
TCTCCTCTATCTGTGTCTTGAAGTATGGCGGGTAGAAGTATGACGGGTATCCGTCATAAAACAGAATGAGCCAAAAAGGT
GCAACCTGCGTTTAGGCGCG

Product: amidophosphoribosyltransferase

Products: NA

Alternate protein names: ATase; Glutamine phosphoribosylpyrophosphate amidotransferase; GPATase [H]

Number of amino acids: Translated: 505; Mature: 505

Protein sequence:

>505_residues
MCGIVGIAGFTPVNQSIYDALMVLQHRGQDAAGIVTIDAHNGFRLRKANGLVKDVFETRHMLRLQGNMGIGHVRYPTAGS
SSASEAQPFYVNSPFGITLAHNGNLTNAHQLRKKLFEVSRRHVNTTSDSEILLNIFASELDRFQHYPLESDNIFAAVAAT
HQLIRGAYACVAMIIGHGMVAFRDPNGIRPLVIGKRTLADGRNEYMVASESVALDTLDFEFLRDVAPGEAVYITEKGQLF
TRQCAENPKYNPCLFEYVYFARPDSFMDKISVYSARVRMGQKLGTKIAKQWEDMDIDVVIPIPETSCDIALEIARILDKP
YRQGFVKNRYVGRTFIMPGQQERRKSVRRKLNANRAEFRGKNVLLVDDSIVRGTTSEQIVEMAREAGAKKVYFASAAPEI
RFPNVYGIDMPSANELIAHGREVDEIRQLIGADALIFQDLTDLIDAVREDNPDITQFECSVFNGIYVTKDVDQSYLEYLE
SLRNDDAQALRSHNEAENLEMHNEG

Sequences:

>Translated_505_residues
MCGIVGIAGFTPVNQSIYDALMVLQHRGQDAAGIVTIDAHNGFRLRKANGLVKDVFETRHMLRLQGNMGIGHVRYPTAGS
SSASEAQPFYVNSPFGITLAHNGNLTNAHQLRKKLFEVSRRHVNTTSDSEILLNIFASELDRFQHYPLESDNIFAAVAAT
HQLIRGAYACVAMIIGHGMVAFRDPNGIRPLVIGKRTLADGRNEYMVASESVALDTLDFEFLRDVAPGEAVYITEKGQLF
TRQCAENPKYNPCLFEYVYFARPDSFMDKISVYSARVRMGQKLGTKIAKQWEDMDIDVVIPIPETSCDIALEIARILDKP
YRQGFVKNRYVGRTFIMPGQQERRKSVRRKLNANRAEFRGKNVLLVDDSIVRGTTSEQIVEMAREAGAKKVYFASAAPEI
RFPNVYGIDMPSANELIAHGREVDEIRQLIGADALIFQDLTDLIDAVREDNPDITQFECSVFNGIYVTKDVDQSYLEYLE
SLRNDDAQALRSHNEAENLEMHNEG
>Mature_505_residues
MCGIVGIAGFTPVNQSIYDALMVLQHRGQDAAGIVTIDAHNGFRLRKANGLVKDVFETRHMLRLQGNMGIGHVRYPTAGS
SSASEAQPFYVNSPFGITLAHNGNLTNAHQLRKKLFEVSRRHVNTTSDSEILLNIFASELDRFQHYPLESDNIFAAVAAT
HQLIRGAYACVAMIIGHGMVAFRDPNGIRPLVIGKRTLADGRNEYMVASESVALDTLDFEFLRDVAPGEAVYITEKGQLF
TRQCAENPKYNPCLFEYVYFARPDSFMDKISVYSARVRMGQKLGTKIAKQWEDMDIDVVIPIPETSCDIALEIARILDKP
YRQGFVKNRYVGRTFIMPGQQERRKSVRRKLNANRAEFRGKNVLLVDDSIVRGTTSEQIVEMAREAGAKKVYFASAAPEI
RFPNVYGIDMPSANELIAHGREVDEIRQLIGADALIFQDLTDLIDAVREDNPDITQFECSVFNGIYVTKDVDQSYLEYLE
SLRNDDAQALRSHNEAENLEMHNEG

Specific function: De novo purine biosynthesis; first step. [C]

COG id: COG0034

COG function: function code F; Glutamine phosphoribosylpyrophosphate amidotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI29570798, Length=476, Percent_Identity=37.3949579831933, Blast_Score=277, Evalue=2e-74,
Organism=Escherichia coli, GI1788651, Length=505, Percent_Identity=87.1287128712871, Blast_Score=926, Evalue=0.0,
Organism=Escherichia coli, GI1790167, Length=250, Percent_Identity=30.8, Blast_Score=75, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17554892, Length=464, Percent_Identity=33.8362068965517, Blast_Score=238, Evalue=7e-63,
Organism=Saccharomyces cerevisiae, GI6323958, Length=489, Percent_Identity=53.9877300613497, Blast_Score=520, Evalue=1e-148,
Organism=Drosophila melanogaster, GI24659598, Length=476, Percent_Identity=34.453781512605, Blast_Score=253, Evalue=2e-67,
Organism=Drosophila melanogaster, GI24659604, Length=469, Percent_Identity=34.5415778251599, Blast_Score=251, Evalue=7e-67,
Organism=Drosophila melanogaster, GI28573187, Length=480, Percent_Identity=34.7916666666667, Blast_Score=246, Evalue=2e-65,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005854
- InterPro:   IPR000583
- InterPro:   IPR017932
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00310 GATase_2; PF00156 Pribosyltran [H]

EC number: =2.4.2.14 [H]

Molecular weight: Translated: 56668; Mature: 56668

Theoretical pI: Translated: 6.21; Mature: 6.21

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER ; PS00443 GATASE_TYPE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCGIVGIAGFTPVNQSIYDALMVLQHRGQDAAGIVTIDAHNGFRLRKANGLVKDVFETRH
CCCEEECCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEECCCCEEEEHHCCHHHHHHHHHH
MLRLQGNMGIGHVRYPTAGSSSASEAQPFYVNSPFGITLAHNGNLTNAHQLRKKLFEVSR
HEEEECCCCCCEEECCCCCCCCCCCCCCEEECCCCEEEEEECCCCCCHHHHHHHHHHHHH
RHVNTTSDSEILLNIFASELDRFQHYPLESDNIFAAVAATHQLIRGAYACVAMIIGHGMV
HHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHCCEE
AFRDPNGIRPLVIGKRTLADGRNEYMVASESVALDTLDFEFLRDVAPGEAVYITEKGQLF
EEECCCCCCEEEECCHHHCCCCCCEEEEECCEEEEHHHHHHHHHCCCCCEEEEECCCCHH
TRQCAENPKYNPCLFEYVYFARPDSFMDKISVYSARVRMGQKLGTKIAKQWEDMDIDVVI
HHHHHCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEE
PIPETSCDIALEIARILDKPYRQGFVKNRYVGRTFIMPGQQERRKSVRRKLNANRAEFRG
ECCCCCHHHHHHHHHHHCCHHHCCHHHCCCCCEEEECCCHHHHHHHHHHHHCCCHHHHCC
KNVLLVDDSIVRGTTSEQIVEMAREAGAKKVYFASAAPEIRFPNVYGIDMPSANELIAHG
CEEEEECCCHHCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCCCEEECCCCCHHHHHHCC
REVDEIRQLIGADALIFQDLTDLIDAVREDNPDITQFECSVFNGIYVTKDVDQSYLEYLE
CCHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCEEEEECCCHHHHHHHH
SLRNDDAQALRSHNEAENLEMHNEG
HHCCCHHHHHHCCCCHHCCCCCCCC
>Mature Secondary Structure
MCGIVGIAGFTPVNQSIYDALMVLQHRGQDAAGIVTIDAHNGFRLRKANGLVKDVFETRH
CCCEEECCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEECCCCEEEEHHCCHHHHHHHHHH
MLRLQGNMGIGHVRYPTAGSSSASEAQPFYVNSPFGITLAHNGNLTNAHQLRKKLFEVSR
HEEEECCCCCCEEECCCCCCCCCCCCCCEEECCCCEEEEEECCCCCCHHHHHHHHHHHHH
RHVNTTSDSEILLNIFASELDRFQHYPLESDNIFAAVAATHQLIRGAYACVAMIIGHGMV
HHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHCCEE
AFRDPNGIRPLVIGKRTLADGRNEYMVASESVALDTLDFEFLRDVAPGEAVYITEKGQLF
EEECCCCCCEEEECCHHHCCCCCCEEEEECCEEEEHHHHHHHHHCCCCCEEEEECCCCHH
TRQCAENPKYNPCLFEYVYFARPDSFMDKISVYSARVRMGQKLGTKIAKQWEDMDIDVVI
HHHHHCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEE
PIPETSCDIALEIARILDKPYRQGFVKNRYVGRTFIMPGQQERRKSVRRKLNANRAEFRG
ECCCCCHHHHHHHHHHHCCHHHCCHHHCCCCCEEEECCCHHHHHHHHHHHHCCCHHHHCC
KNVLLVDDSIVRGTTSEQIVEMAREAGAKKVYFASAAPEIRFPNVYGIDMPSANELIAHG
CEEEEECCCHHCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCCCEEECCCCCHHHHHHCC
REVDEIRQLIGADALIFQDLTDLIDAVREDNPDITQFECSVFNGIYVTKDVDQSYLEYLE
CCHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCEEEEECCCHHHHHHHH
SLRNDDAQALRSHNEAENLEMHNEG
HHCCCHHHHHHCCCCHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3047685; 6443594; 6277938; 9205837; 9278503; 3040734; 7037784; 9333323; 9514258 [H]