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The map label for this gene is nadB [H]

Identifier: 218929782

GI number: 218929782

Start: 3037547

End: 3039184

Strand: Reverse

Name: nadB [H]

Synonym: YPO2710

Alternate gene names: 218929782

Gene position: 3039184-3037547 (Counterclockwise)

Preceding gene: 218929787

Following gene: 218929780

Centisome position: 65.31

GC content: 48.41

Gene sequence:

>1638_bases
ATGAAACCAACTCTTCTTCTGCACACATCATACGATATGCAATCATCATCTGAACACATTAGCGATGTATTGATCATCGG
GAGTGGCGCTGCTGGCTTGTCTTTAGCTCTCCGCCTCGCGCCACATTGTAAAGTTACCGTTCTGAGCAAAGGGCCTCTTA
ATGAGGGAGCCACTTTTTATGCTCAGGGAGGTATTGCCGCTGTTTTCGACGAAACTGACAGTATCAGTTCACATGTAGAT
GACACCTTAATCGCCGGTGCCGGGCTTTGTGACAAAGAAGCCGTTGAATTTATTGCCAGCAATGCCCGCTCTTGTGTGCA
GTGGCTCATTGATCAAGGCGTTCTTTTTGATACAGAGACCAGTGCTAACGGTGAAGAGCGCTATCACCTTACCCGTGAAG
GTGGTCATAGCCACCGCCGTATTCTGCATACAGCGGATGCTACCGGTAAAGAAGTAGAAACTACCTTAGTGGGTAAAGCA
AGCAATCACCCTAATATTTCAGTGAAAGAACGCTGCAATGCTGTCGATCTTATCACATCAAACAAAATTGGTTTGGCGGG
TACCAAACGCGTAGTGGGTGCTTATGTCTGGAACCGTGAGTTAGAGAAAGTTGAGACTTTCCGCGCCAAAGCCGTGGTTT
TGGCGACAGGCGGCGCGGCTAAAGTTTATCAATACACCACTAACCCAGATATTTCGTCTGGTGATGGTATTGCCATGGCG
TGGCGTGCCGGGTGCCGGGTCGCTAATTTGGAATTTAACCAATTTCATCCAACCTGTCTCTTCCACCCGCAAGCACGTAA
TTTTTTACTGACAGAAGCCTTACGTGGTGAAGGTGCCTACCTTAAACGCCCTGATGGCAGCCGTTTCATGCTTGATTTTG
ATCCACGCGGTGAACTTGCGCCACGTGATATCGTTGCCCGAGCCATCGACCATGAAATGAAGCGCCTCGGTGCTGATTGC
ATGTATCTGGATATCAGCCATAAACCTACTGATTTCGTGATGCAACATTTCCCAATGATCTATGAAAAACTGCTGTCATT
GGGTATCGATCTAACTAAAGAAGCTATTCCGATTGTCCCCGCAGCTCATTACACCTGTGGTGGCGTAATGGTTGATCAAC
ATGGGCGTACCGATTTAGATGGGTTATATGCGATTGGTGAAGTGAGCTACACCGGCTTACACGGTGCCAACCGCATGGCC
TCCAATTCCCTGCTCGAATGCCTGGTCTATGGCTGGTCAGCAGCAGAAGACATTCTGACACGGCTGCCGACGGCCAAACT
GGCCAAGCATCTGCCAGATTGGGATGAAAGCCGCGTTGATAATTCTGACGAACGGGTTGTCATTCAGCATAACTGGCATG
AGTTGCGTTTATTTATGTGGGATTACGTCGGCATTGTCCGTACCACAAAACGCTTAGAGCGGGCTTTACGGCGCATTAAC
ACGTTGCAGCAAGAGATTGATGAATATTATGCCAATTTCCGTATCTCCAATAACCTGCTGGAGTTACGGAATCTGGTACA
AGTCGCGGAGTTAATCGTGCGCTGTGCGATGGAACGTAAAGAGAGCCGTGGGTTGCACTATACGCTTGATTACCCTGATC
AAATAGAAAACCCACAGCCAACCATTCTGCATCCATAA

Upstream 100 bases:

>100_bases
AGTTTATAAAAATTGTAAAGTTCCATGTTGCCCGATTTTTTGTCCAAATATTATTCAGACATCTTATACCCATAGCTTTT
TCTTGAGCAGATAGGCTAAC

Downstream 100 bases:

>100_bases
CACATTCTGAATCCATAAGATTGAGGGCGTCTCTACGACGCCCGCAGACTGCTGACAAACCCCGATGACGCGATCTTGAA
CGGTGAGGATAGGCAGAGGA

Product: L-aspartate oxidase

Products: NA

Alternate protein names: LASPO; Quinolinate synthase B [H]

Number of amino acids: Translated: 545; Mature: 545

Protein sequence:

>545_residues
MKPTLLLHTSYDMQSSSEHISDVLIIGSGAAGLSLALRLAPHCKVTVLSKGPLNEGATFYAQGGIAAVFDETDSISSHVD
DTLIAGAGLCDKEAVEFIASNARSCVQWLIDQGVLFDTETSANGEERYHLTREGGHSHRRILHTADATGKEVETTLVGKA
SNHPNISVKERCNAVDLITSNKIGLAGTKRVVGAYVWNRELEKVETFRAKAVVLATGGAAKVYQYTTNPDISSGDGIAMA
WRAGCRVANLEFNQFHPTCLFHPQARNFLLTEALRGEGAYLKRPDGSRFMLDFDPRGELAPRDIVARAIDHEMKRLGADC
MYLDISHKPTDFVMQHFPMIYEKLLSLGIDLTKEAIPIVPAAHYTCGGVMVDQHGRTDLDGLYAIGEVSYTGLHGANRMA
SNSLLECLVYGWSAAEDILTRLPTAKLAKHLPDWDESRVDNSDERVVIQHNWHELRLFMWDYVGIVRTTKRLERALRRIN
TLQQEIDEYYANFRISNNLLELRNLVQVAELIVRCAMERKESRGLHYTLDYPDQIENPQPTILHP

Sequences:

>Translated_545_residues
MKPTLLLHTSYDMQSSSEHISDVLIIGSGAAGLSLALRLAPHCKVTVLSKGPLNEGATFYAQGGIAAVFDETDSISSHVD
DTLIAGAGLCDKEAVEFIASNARSCVQWLIDQGVLFDTETSANGEERYHLTREGGHSHRRILHTADATGKEVETTLVGKA
SNHPNISVKERCNAVDLITSNKIGLAGTKRVVGAYVWNRELEKVETFRAKAVVLATGGAAKVYQYTTNPDISSGDGIAMA
WRAGCRVANLEFNQFHPTCLFHPQARNFLLTEALRGEGAYLKRPDGSRFMLDFDPRGELAPRDIVARAIDHEMKRLGADC
MYLDISHKPTDFVMQHFPMIYEKLLSLGIDLTKEAIPIVPAAHYTCGGVMVDQHGRTDLDGLYAIGEVSYTGLHGANRMA
SNSLLECLVYGWSAAEDILTRLPTAKLAKHLPDWDESRVDNSDERVVIQHNWHELRLFMWDYVGIVRTTKRLERALRRIN
TLQQEIDEYYANFRISNNLLELRNLVQVAELIVRCAMERKESRGLHYTLDYPDQIENPQPTILHP
>Mature_545_residues
MKPTLLLHTSYDMQSSSEHISDVLIIGSGAAGLSLALRLAPHCKVTVLSKGPLNEGATFYAQGGIAAVFDETDSISSHVD
DTLIAGAGLCDKEAVEFIASNARSCVQWLIDQGVLFDTETSANGEERYHLTREGGHSHRRILHTADATGKEVETTLVGKA
SNHPNISVKERCNAVDLITSNKIGLAGTKRVVGAYVWNRELEKVETFRAKAVVLATGGAAKVYQYTTNPDISSGDGIAMA
WRAGCRVANLEFNQFHPTCLFHPQARNFLLTEALRGEGAYLKRPDGSRFMLDFDPRGELAPRDIVARAIDHEMKRLGADC
MYLDISHKPTDFVMQHFPMIYEKLLSLGIDLTKEAIPIVPAAHYTCGGVMVDQHGRTDLDGLYAIGEVSYTGLHGANRMA
SNSLLECLVYGWSAAEDILTRLPTAKLAKHLPDWDESRVDNSDERVVIQHNWHELRLFMWDYVGIVRTTKRLERALRRIN
TLQQEIDEYYANFRISNNLLELRNLVQVAELIVRCAMERKESRGLHYTLDYPDQIENPQPTILHP

Specific function: Catalyzes the oxidation of L-aspartate to iminoaspartate [H]

COG id: COG0029

COG function: function code H; Aspartate oxidase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAD-dependent oxidoreductase 2 family. NadB subfamily [H]

Homologues:

Organism=Homo sapiens, GI156416003, Length=516, Percent_Identity=31.2015503875969, Blast_Score=189, Evalue=7e-48,
Organism=Escherichia coli, GI1788928, Length=533, Percent_Identity=83.8649155722326, Blast_Score=928, Evalue=0.0,
Organism=Escherichia coli, GI1790597, Length=524, Percent_Identity=32.4427480916031, Blast_Score=229, Evalue=5e-61,
Organism=Escherichia coli, GI1786942, Length=532, Percent_Identity=30.8270676691729, Blast_Score=179, Evalue=6e-46,
Organism=Caenorhabditis elegans, GI17550100, Length=544, Percent_Identity=31.4338235294118, Blast_Score=205, Evalue=5e-53,
Organism=Caenorhabditis elegans, GI17505833, Length=539, Percent_Identity=30.6122448979592, Blast_Score=203, Evalue=2e-52,
Organism=Caenorhabditis elegans, GI71986328, Length=467, Percent_Identity=27.8372591006424, Blast_Score=103, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6322701, Length=528, Percent_Identity=32.3863636363636, Blast_Score=214, Evalue=3e-56,
Organism=Saccharomyces cerevisiae, GI6322416, Length=530, Percent_Identity=31.1320754716981, Blast_Score=202, Evalue=2e-52,
Organism=Saccharomyces cerevisiae, GI6320788, Length=485, Percent_Identity=27.0103092783505, Blast_Score=112, Evalue=1e-25,
Organism=Drosophila melanogaster, GI17137288, Length=540, Percent_Identity=31.1111111111111, Blast_Score=190, Evalue=2e-48,
Organism=Drosophila melanogaster, GI24655642, Length=540, Percent_Identity=31.1111111111111, Blast_Score=190, Evalue=2e-48,
Organism=Drosophila melanogaster, GI24655647, Length=540, Percent_Identity=31.1111111111111, Blast_Score=190, Evalue=2e-48,
Organism=Drosophila melanogaster, GI24663005, Length=565, Percent_Identity=29.3805309734513, Blast_Score=180, Evalue=2e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003953
- InterPro:   IPR013027
- InterPro:   IPR015939
- InterPro:   IPR004112
- InterPro:   IPR005288 [H]

Pfam domain/function: PF00890 FAD_binding_2; PF02910 Succ_DH_flav_C [H]

EC number: =1.4.3.16 [H]

Molecular weight: Translated: 60696; Mature: 60696

Theoretical pI: Translated: 6.36; Mature: 6.36

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPTLLLHTSYDMQSSSEHISDVLIIGSGAAGLSLALRLAPHCKVTVLSKGPLNEGATFY
CCCEEEEEECCCCCCCHHCCCEEEEEECCCCCHHHHEEECCCCEEEEEECCCCCCCCEEE
AQGGIAAVFDETDSISSHVDDTLIAGAGLCDKEAVEFIASNARSCVQWLIDQGVLFDTET
ECCCEEEEECCCCHHHHHCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCEEEECCC
SANGEERYHLTREGGHSHRRILHTADATGKEVETTLVGKASNHPNISVKERCNAVDLITS
CCCCCCEEEEECCCCCCCCEEEEECCCCCCEEEEEEEECCCCCCCEEHHHHCCEEEEEEC
NKIGLAGTKRVVGAYVWNRELEKVETFRAKAVVLATGGAAKVYQYTTNPDISSGDGIAMA
CCCCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCEEEEEECCCCCCCCCCEEEE
WRAGCRVANLEFNQFHPTCLFHPQARNFLLTEALRGEGAYLKRPDGSRFMLDFDPRGELA
ECCCCEEEEEECCCCCCEEEECCCCCCCHHHHHHCCCCCEEECCCCCEEEEEECCCCCCC
PRDIVARAIDHEMKRLGADCMYLDISHKPTDFVMQHFPMIYEKLLSLGIDLTKEAIPIVP
HHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
AAHYTCGGVMVDQHGRTDLDGLYAIGEVSYTGLHGANRMASNSLLECLVYGWSAAEDILT
CCCCCCCCEEEECCCCCCCCCEEEECCEEECCCCCCHHHHHHHHHHHHHHCCCHHHHHHH
RLPTAKLAKHLPDWDESRVDNSDERVVIQHNWHELRLFMWDYVGIVRTTKRLERALRRIN
HCCHHHHHHHCCCCCHHHCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
TLQQEIDEYYANFRISNNLLELRNLVQVAELIVRCAMERKESRGLHYTLDYPDQIENPQP
HHHHHHHHHHHCEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCHHCCCCCC
TILHP
CCCCC
>Mature Secondary Structure
MKPTLLLHTSYDMQSSSEHISDVLIIGSGAAGLSLALRLAPHCKVTVLSKGPLNEGATFY
CCCEEEEEECCCCCCCHHCCCEEEEEECCCCCHHHHEEECCCCEEEEEECCCCCCCCEEE
AQGGIAAVFDETDSISSHVDDTLIAGAGLCDKEAVEFIASNARSCVQWLIDQGVLFDTET
ECCCEEEEECCCCHHHHHCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCEEEECCC
SANGEERYHLTREGGHSHRRILHTADATGKEVETTLVGKASNHPNISVKERCNAVDLITS
CCCCCCEEEEECCCCCCCCEEEEECCCCCCEEEEEEEECCCCCCCEEHHHHCCEEEEEEC
NKIGLAGTKRVVGAYVWNRELEKVETFRAKAVVLATGGAAKVYQYTTNPDISSGDGIAMA
CCCCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCEEEEEECCCCCCCCCCEEEE
WRAGCRVANLEFNQFHPTCLFHPQARNFLLTEALRGEGAYLKRPDGSRFMLDFDPRGELA
ECCCCEEEEEECCCCCCEEEECCCCCCCHHHHHHCCCCCEEECCCCCEEEEEECCCCCCC
PRDIVARAIDHEMKRLGADCMYLDISHKPTDFVMQHFPMIYEKLLSLGIDLTKEAIPIVP
HHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
AAHYTCGGVMVDQHGRTDLDGLYAIGEVSYTGLHGANRMASNSLLECLVYGWSAAEDILT
CCCCCCCCEEEECCCCCCCCCEEEECCEEECCCCCCHHHHHHHHHHHHHHCCCHHHHHHH
RLPTAKLAKHLPDWDESRVDNSDERVVIQHNWHELRLFMWDYVGIVRTTKRLERALRRIN
HCCHHHHHHHCCCCCHHHCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
TLQQEIDEYYANFRISNNLLELRNLVQVAELIVRCAMERKESRGLHYTLDYPDQIENPQP
HHHHHHHHHHHCEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCHHCCCCCC
TILHP
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11586360; 12142430 [H]