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The map label for this gene is nrdE [H]

Identifier: 218929726

GI number: 218929726

Start: 2976950

End: 2979031

Strand: Reverse

Name: nrdE [H]

Synonym: YPO2649

Alternate gene names: 218929726

Gene position: 2979031-2976950 (Counterclockwise)

Preceding gene: 218929727

Following gene: 218929725

Centisome position: 64.01

GC content: 46.64

Gene sequence:

>2082_bases
ATGCTGAACCTTTATGATGCTGATGGGCGTATCCAGTTTGATAAAGACCGGCTGGCCGCACGGCAATACTTTTTGCAGCA
CGTTAATCAAAACACGGTGTTCTTCCATAATCTACAGGAAAAAATTGATTACCTGATTGAGGAGGGATATTACGAAAAAG
AGGTTCTGGATCAATACGATTTTAGCTTTATCAAAAGTCTATTCCAGCAAGCCTATAGCAAAAAATTTCGTTTCCCCACT
TTTCTCGGGGCGTTTAAATATTACACCAGCTACACCCTAAAAACCTTTGATGGCAAACGTTACCTGGAACGCTATGAAGA
TCGCGTTTGCATGGTCGCACTGACACTGGCTACAGGTAACCAGACACTGGCGCGGGAACTGGTAGAAGAAATTATCTCTG
GCCGCTTCCAACCCGCCACACCAACCTTCCTCAATTGTGGCAAAAAACAACGTGGTGAACTGGTCTCCTGCTTCCTGTTG
CGTATCGAAGACAATATGGAATCCATTGGCCGCTCGATTAACTCGGCACTGCAACTCTCTAAACGTGGCGGAGGCGTGGC
GTTCCTCCTCAGCAATATCCGTGAGGTGGGTGCTCCCATCAAACGGATTGAAAATCAGTCTTCGGGTGTTATCCCGATAA
TGAAAATGTTGGAGGACGCGTTCTCCTATGCCAATCAGTTGGGTGCCCGTCAGGGGGCGGGGGCGGTCTATCTCCATGCC
CATCATCCAGATATTTTGCGTTTTCTGGATACCAAGCGGGAAAATGCCGATGAAAAAGTACGGATTAAGACCTTATCGTT
GGGGGTGGTGATACCGGATATCACTTTTGAACTGGCGAAGAATAATGAAGAAATGTACCTGTTTTCACCTTATGACATTG
AACAGGTTTACGGCGTGCCCATGTCTGAAATCAGCATCAGCGAAAAATACCGCGAAATGGTGGATAACAAACGCATCCGC
AAAAGCAAAATTAAAGCCCGCGAGTTCTTCCAAATTCTGGCTGAGATCCAATTTGAATCTGGCTACCCTTATATGATGTT
TGAAGATACGGTTAATCGCGCTAATCCGATTAAAGGCCGGATTAATATGAGTAATTTGTGTTCGGAAATTTTGCAGGTCA
ACGCCCCCACTGAATACCACGAAGACCTCAGCTATCGCCGTATCGGGAAAGATATCTCCTGTAATCTTGGCTCGCTGAAT
ATCGCGCAGGTGATGGATTCACCCGATTTTGGTAAATCCATTGAGATCGCCATTCGTGGGCTCACTGCCGTATCTGAAAT
GAGCAATATTCGCTCAGTGCCATCCATTGAAAAAGGCAATCAAGAATCACATGCCATTGGCCTTGGGCAGATGAACCTGC
ACGGTTATCTGGCGCGGGAGCGTCTTTTTTACGGCTCGGAAGAGGCGTTGGATTTTACCAATATCTATTTTTATACCGTG
GCGTATCACGCGATCCGTGCCTCAAACCAGTTGGCAATAGAACGGGCCAGCCATTTCAAAGGCTTCGATGAATCAGATTA
TGCTTCGGGTGCTTTCTTCAGCAAATATATCGAGCAAAGCTGGCAGCCAAAAACAGCAAGAGTACAGGCACTGTTTGCCG
ATGCCGGTATTGCCATACCAAACCAACAAGATTGGATCGCGCTACGGCAATCCGTTATGGAACACGGTATTTATAATCAG
AACCTACAGGCGGTGCCACCAACAGGCTCTATTTCCTATATCAATCATTCGACATCAAGTATCCACCCGATTGTCTCTCC
GATTGAGATCCGTAAAGAAGGCAAAATAGGCCGTGTTTATTATCCCGCACCCTATATGACCAACGATAACCTTGAATATT
ATCAAGATGCCTACCAGATCGGGCCTGAAAAAATCATCGATACCTATGCCGCAGCGACCCAACATGTTGATCAGGGCCTC
TCTCTGACGCTGTTCTTCCGTGATACCGCTACCACCCGCGATATTAACAAAGCACAGATTTACGCTTGGAAGAAAGGGAT
CAAGACCATTTATTACATCCGGCTACGTCAGATGGCATTAGAGGGAACCGAAGTCGAGGGCTGCGTTTCCTGTTCGCTGT
AA

Upstream 100 bases:

>100_bases
TGGCAAACGTTCGCAAGGGAGTAACTGAATTTTGGCAACGACAAAACTGAACGTCATCGATTCATCCGACAGTGAGCTGG
ATTATCACTCACTGAATGCA

Downstream 100 bases:

>100_bases
TGTCTTTTCTGTTTACGGCAACTTTTCTGTTTACGGTAACTTTCCTGCTCACGGTAAATAGATAGCACTGAATTAATCAG
GTCGGATAGATTAAGGAAAC

Product: ribonucleotide-diphosphate reductase subunit alpha

Products: NA

Alternate protein names: Ribonucleotide reductase R1 subunit [H]

Number of amino acids: Translated: 693; Mature: 693

Protein sequence:

>693_residues
MLNLYDADGRIQFDKDRLAARQYFLQHVNQNTVFFHNLQEKIDYLIEEGYYEKEVLDQYDFSFIKSLFQQAYSKKFRFPT
FLGAFKYYTSYTLKTFDGKRYLERYEDRVCMVALTLATGNQTLARELVEEIISGRFQPATPTFLNCGKKQRGELVSCFLL
RIEDNMESIGRSINSALQLSKRGGGVAFLLSNIREVGAPIKRIENQSSGVIPIMKMLEDAFSYANQLGARQGAGAVYLHA
HHPDILRFLDTKRENADEKVRIKTLSLGVVIPDITFELAKNNEEMYLFSPYDIEQVYGVPMSEISISEKYREMVDNKRIR
KSKIKAREFFQILAEIQFESGYPYMMFEDTVNRANPIKGRINMSNLCSEILQVNAPTEYHEDLSYRRIGKDISCNLGSLN
IAQVMDSPDFGKSIEIAIRGLTAVSEMSNIRSVPSIEKGNQESHAIGLGQMNLHGYLARERLFYGSEEALDFTNIYFYTV
AYHAIRASNQLAIERASHFKGFDESDYASGAFFSKYIEQSWQPKTARVQALFADAGIAIPNQQDWIALRQSVMEHGIYNQ
NLQAVPPTGSISYINHSTSSIHPIVSPIEIRKEGKIGRVYYPAPYMTNDNLEYYQDAYQIGPEKIIDTYAAATQHVDQGL
SLTLFFRDTATTRDINKAQIYAWKKGIKTIYYIRLRQMALEGTEVEGCVSCSL

Sequences:

>Translated_693_residues
MLNLYDADGRIQFDKDRLAARQYFLQHVNQNTVFFHNLQEKIDYLIEEGYYEKEVLDQYDFSFIKSLFQQAYSKKFRFPT
FLGAFKYYTSYTLKTFDGKRYLERYEDRVCMVALTLATGNQTLARELVEEIISGRFQPATPTFLNCGKKQRGELVSCFLL
RIEDNMESIGRSINSALQLSKRGGGVAFLLSNIREVGAPIKRIENQSSGVIPIMKMLEDAFSYANQLGARQGAGAVYLHA
HHPDILRFLDTKRENADEKVRIKTLSLGVVIPDITFELAKNNEEMYLFSPYDIEQVYGVPMSEISISEKYREMVDNKRIR
KSKIKAREFFQILAEIQFESGYPYMMFEDTVNRANPIKGRINMSNLCSEILQVNAPTEYHEDLSYRRIGKDISCNLGSLN
IAQVMDSPDFGKSIEIAIRGLTAVSEMSNIRSVPSIEKGNQESHAIGLGQMNLHGYLARERLFYGSEEALDFTNIYFYTV
AYHAIRASNQLAIERASHFKGFDESDYASGAFFSKYIEQSWQPKTARVQALFADAGIAIPNQQDWIALRQSVMEHGIYNQ
NLQAVPPTGSISYINHSTSSIHPIVSPIEIRKEGKIGRVYYPAPYMTNDNLEYYQDAYQIGPEKIIDTYAAATQHVDQGL
SLTLFFRDTATTRDINKAQIYAWKKGIKTIYYIRLRQMALEGTEVEGCVSCSL
>Mature_693_residues
MLNLYDADGRIQFDKDRLAARQYFLQHVNQNTVFFHNLQEKIDYLIEEGYYEKEVLDQYDFSFIKSLFQQAYSKKFRFPT
FLGAFKYYTSYTLKTFDGKRYLERYEDRVCMVALTLATGNQTLARELVEEIISGRFQPATPTFLNCGKKQRGELVSCFLL
RIEDNMESIGRSINSALQLSKRGGGVAFLLSNIREVGAPIKRIENQSSGVIPIMKMLEDAFSYANQLGARQGAGAVYLHA
HHPDILRFLDTKRENADEKVRIKTLSLGVVIPDITFELAKNNEEMYLFSPYDIEQVYGVPMSEISISEKYREMVDNKRIR
KSKIKAREFFQILAEIQFESGYPYMMFEDTVNRANPIKGRINMSNLCSEILQVNAPTEYHEDLSYRRIGKDISCNLGSLN
IAQVMDSPDFGKSIEIAIRGLTAVSEMSNIRSVPSIEKGNQESHAIGLGQMNLHGYLARERLFYGSEEALDFTNIYFYTV
AYHAIRASNQLAIERASHFKGFDESDYASGAFFSKYIEQSWQPKTARVQALFADAGIAIPNQQDWIALRQSVMEHGIYNQ
NLQAVPPTGSISYINHSTSSIHPIVSPIEIRKEGKIGRVYYPAPYMTNDNLEYYQDAYQIGPEKIIDTYAAATQHVDQGL
SLTLFFRDTATTRDINKAQIYAWKKGIKTIYYIRLRQMALEGTEVEGCVSCSL

Specific function: Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides [H]

COG id: COG0209

COG function: function code F; Ribonucleotide reductase, alpha subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ribonucleoside diphosphate reductase large chain family [H]

Homologues:

Organism=Homo sapiens, GI4506749, Length=656, Percent_Identity=26.219512195122, Blast_Score=185, Evalue=1e-46,
Organism=Escherichia coli, GI1789030, Length=693, Percent_Identity=74.1702741702742, Blast_Score=1108, Evalue=0.0,
Organism=Escherichia coli, GI2367133, Length=560, Percent_Identity=25.1785714285714, Blast_Score=167, Evalue=2e-42,
Organism=Caenorhabditis elegans, GI17555242, Length=652, Percent_Identity=26.9938650306748, Blast_Score=191, Evalue=1e-48,
Organism=Saccharomyces cerevisiae, GI6320914, Length=654, Percent_Identity=26.2996941896024, Blast_Score=189, Evalue=2e-48,
Organism=Saccharomyces cerevisiae, GI6322123, Length=654, Percent_Identity=25.9938837920489, Blast_Score=181, Evalue=3e-46,
Organism=Drosophila melanogaster, GI17352481, Length=654, Percent_Identity=25.5351681957187, Blast_Score=171, Evalue=1e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013346
- InterPro:   IPR013509
- InterPro:   IPR000788
- InterPro:   IPR008926
- InterPro:   IPR013554 [H]

Pfam domain/function: PF02867 Ribonuc_red_lgC; PF00317 Ribonuc_red_lgN; PF08343 RNR_N [H]

EC number: =1.17.4.1 [H]

Molecular weight: Translated: 79182; Mature: 79182

Theoretical pI: Translated: 6.76; Mature: 6.76

Prosite motif: PS00089 RIBORED_LARGE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLNLYDADGRIQFDKDRLAARQYFLQHVNQNTVFFHNLQEKIDYLIEEGYYEKEVLDQYD
CCEEECCCCEEEECHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHCCCHHHHHHHHCC
FSFIKSLFQQAYSKKFRFPTFLGAFKYYTSYTLKTFDGKRYLERYEDRVCMVALTLATGN
HHHHHHHHHHHHHCCCCCHHHHHHHHHHHHEEEEECCCHHHHHHHHCCEEEEEEEEECCC
QTLARELVEEIISGRFQPATPTFLNCGKKQRGELVSCFLLRIEDNMESIGRSINSALQLS
HHHHHHHHHHHHCCCCCCCCCHHHHCCCHHHCCCEEHHHHHHHHHHHHHHHHHHHHHHHH
KRGGGVAFLLSNIREVGAPIKRIENQSSGVIPIMKMLEDAFSYANQLGARQGAGAVYLHA
CCCCCHHHHHHHHHHHCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEE
HHPDILRFLDTKRENADEKVRIKTLSLGVVIPDITFELAKNNEEMYLFSPYDIEQVYGVP
CCHHHHHHHHHHCCCCCCEEEEEEEEEEEEECCCEEEEECCCCEEEEECCCCHHHHCCCC
MSEISISEKYREMVDNKRIRKSKIKAREFFQILAEIQFESGYPYMMFEDTVNRANPIKGR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCC
INMSNLCSEILQVNAPTEYHEDLSYRRIGKDISCNLGSLNIAQVMDSPDFGKSIEIAIRG
CCHHHHHHHHHHCCCCCHHHHCCHHHHCCCCCCCCCCCCCHHHHHCCCCCCCEEEEEEEH
LTAVSEMSNIRSVPSIEKGNQESHAIGLGQMNLHGYLARERLFYGSEEALDFTNIYFYTV
HHHHHHHHHHHCCCCCCCCCCCHHEEEECCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHH
AYHAIRASNQLAIERASHFKGFDESDYASGAFFSKYIEQSWQPKTARVQALFADAGIAIP
HHHHHHCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHEEEEECCCEECC
NQQDWIALRQSVMEHGIYNQNLQAVPPTGSISYINHSTSSIHPIVSPIEIRKEGKIGRVY
CCHHHHHHHHHHHHCCCCCCCCEECCCCCCEEEEECCCCCCCCCCCCHHHHCCCCCCEEE
YPAPYMTNDNLEYYQDAYQIGPEKIIDTYAAATQHVDQGLSLTLFFRDTATTRDINKAQI
ECCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCHHHH
YAWKKGIKTIYYIRLRQMALEGTEVEGCVSCSL
HHHHHCCHHHHHHHHHHHHHCCCCCCCEEECCC
>Mature Secondary Structure
MLNLYDADGRIQFDKDRLAARQYFLQHVNQNTVFFHNLQEKIDYLIEEGYYEKEVLDQYD
CCEEECCCCEEEECHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHCCCHHHHHHHHCC
FSFIKSLFQQAYSKKFRFPTFLGAFKYYTSYTLKTFDGKRYLERYEDRVCMVALTLATGN
HHHHHHHHHHHHHCCCCCHHHHHHHHHHHHEEEEECCCHHHHHHHHCCEEEEEEEEECCC
QTLARELVEEIISGRFQPATPTFLNCGKKQRGELVSCFLLRIEDNMESIGRSINSALQLS
HHHHHHHHHHHHCCCCCCCCCHHHHCCCHHHCCCEEHHHHHHHHHHHHHHHHHHHHHHHH
KRGGGVAFLLSNIREVGAPIKRIENQSSGVIPIMKMLEDAFSYANQLGARQGAGAVYLHA
CCCCCHHHHHHHHHHHCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEE
HHPDILRFLDTKRENADEKVRIKTLSLGVVIPDITFELAKNNEEMYLFSPYDIEQVYGVP
CCHHHHHHHHHHCCCCCCEEEEEEEEEEEEECCCEEEEECCCCEEEEECCCCHHHHCCCC
MSEISISEKYREMVDNKRIRKSKIKAREFFQILAEIQFESGYPYMMFEDTVNRANPIKGR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCC
INMSNLCSEILQVNAPTEYHEDLSYRRIGKDISCNLGSLNIAQVMDSPDFGKSIEIAIRG
CCHHHHHHHHHHCCCCCHHHHCCHHHHCCCCCCCCCCCCCHHHHHCCCCCCCEEEEEEEH
LTAVSEMSNIRSVPSIEKGNQESHAIGLGQMNLHGYLARERLFYGSEEALDFTNIYFYTV
HHHHHHHHHHHCCCCCCCCCCCHHEEEECCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHH
AYHAIRASNQLAIERASHFKGFDESDYASGAFFSKYIEQSWQPKTARVQALFADAGIAIP
HHHHHHCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHEEEEECCCEECC
NQQDWIALRQSVMEHGIYNQNLQAVPPTGSISYINHSTSSIHPIVSPIEIRKEGKIGRVY
CCHHHHHHHHHHHHCCCCCCCCEECCCCCCEEEEECCCCCCCCCCCCHHHHCCCCCCEEE
YPAPYMTNDNLEYYQDAYQIGPEKIIDTYAAATQHVDQGLSLTLFFRDTATTRDINKAQI
ECCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCHHHH
YAWKKGIKTIYYIRLRQMALEGTEVEGCVSCSL
HHHHHCCHHHHHHHHHHHHHCCCCCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]