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The map label for this gene is ymfC

Identifier: 218928776

GI number: 218928776

Start: 1865466

End: 1866092

Strand: Reverse

Name: ymfC

Synonym: YPO1640

Alternate gene names: 218928776

Gene position: 1866092-1865466 (Counterclockwise)

Preceding gene: 218928778

Following gene: 218928775

Centisome position: 40.1

GC content: 49.28

Gene sequence:

>627_bases
ATGAATAAATTCTCTGTTAAAAATCACAAAGTTAACCGATTCAGCACTAAGGCACAAGCCAGGCAGTCCCCCCCCGCTGC
GCCACGGCGGGTATTGTTATTCAATAAACCTTTTGATGTATTACCGCAATTCACTGATGAGTCTGGACGCACCACTTTGC
AGGGTTTCATCCCTTTTAAGGACGTTTATGCTGCTGGCCGGTTAGATCGCGATAGCGAGGGCTTATTGGTATTAACCAAT
GATGGCAAGTTGCAAGCCAGGCTCACGCAACCGGCGCAAAAAACAGGTAAAGTCTATTTTGTACAAGTAGAAGGGGTCCC
TGACAACGATGCGCTCACACAGCTAAAACGAGGCGTAACGTTGAAAGATGGCCTGACGTTACCCGCAGGCGCGGAGTTAG
TGGCAGAGCCTGAGTGGCTATGGCCGCGCCATCCCCCTATCAGAGAGCGTAAAACCATCCCGACCAGTTGGTTGAAAATA
ACACTTTATGAAGGCCGCAACCGGCAAGTACGTAGAATGACCGCACACGTTGGCTTCCCTACCCTGCGCCTGATTCGTTT
TAGTATGGGAAATTTAAGCCTTGGCAACTTACAGCCCGGCGAATGGAAGGAGATAACTGATGTTTAA

Upstream 100 bases:

>100_bases
GCTTTGTAAGATTCGTGTCAATACTACTTGAATATTCGTTCCGCGCCAATCCGAAACGGATTGGGGTATAATGCGCTAAT
CATTCGAAACCGCAATAATC

Downstream 100 bases:

>100_bases
ACCGCATGTTACTGTCGCCTGTGTCGTTCATACTCAGGGGAAATTTCTGATCGTTGAAGAAACCATCAACAATGAAAAAC
GCTGGAACCAGCCAGCCGGT

Product: putative pseudouridine synthase

Products: NA

Alternate protein names: rRNA pseudouridylate synthase E; rRNA-uridine isomerase E

Number of amino acids: Translated: 208; Mature: 208

Protein sequence:

>208_residues
MNKFSVKNHKVNRFSTKAQARQSPPAAPRRVLLFNKPFDVLPQFTDESGRTTLQGFIPFKDVYAAGRLDRDSEGLLVLTN
DGKLQARLTQPAQKTGKVYFVQVEGVPDNDALTQLKRGVTLKDGLTLPAGAELVAEPEWLWPRHPPIRERKTIPTSWLKI
TLYEGRNRQVRRMTAHVGFPTLRLIRFSMGNLSLGNLQPGEWKEITDV

Sequences:

>Translated_208_residues
MNKFSVKNHKVNRFSTKAQARQSPPAAPRRVLLFNKPFDVLPQFTDESGRTTLQGFIPFKDVYAAGRLDRDSEGLLVLTN
DGKLQARLTQPAQKTGKVYFVQVEGVPDNDALTQLKRGVTLKDGLTLPAGAELVAEPEWLWPRHPPIRERKTIPTSWLKI
TLYEGRNRQVRRMTAHVGFPTLRLIRFSMGNLSLGNLQPGEWKEITDV
>Mature_208_residues
MNKFSVKNHKVNRFSTKAQARQSPPAAPRRVLLFNKPFDVLPQFTDESGRTTLQGFIPFKDVYAAGRLDRDSEGLLVLTN
DGKLQARLTQPAQKTGKVYFVQVEGVPDNDALTQLKRGVTLKDGLTLPAGAELVAEPEWLWPRHPPIRERKTIPTSWLKI
TLYEGRNRQVRRMTAHVGFPTLRLIRFSMGNLSLGNLQPGEWKEITDV

Specific function: Responsible for synthesis of pseudouridine from uracil- 2457 in 23S ribosomal RNA

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase rsuA family

Homologues:

Organism=Escherichia coli, GI87081838, Length=207, Percent_Identity=73.4299516908213, Blast_Score=323, Evalue=7e-90,
Organism=Escherichia coli, GI1787524, Length=187, Percent_Identity=36.3636363636364, Blast_Score=83, Evalue=1e-17,
Organism=Escherichia coli, GI1788510, Length=184, Percent_Identity=32.6086956521739, Blast_Score=81, Evalue=6e-17,
Organism=Escherichia coli, GI1790453, Length=186, Percent_Identity=31.7204301075269, Blast_Score=74, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLUE_YERPE (Q8ZFQ3)

Other databases:

- EMBL:   AL590842
- EMBL:   AE009952
- EMBL:   AE017042
- PIR:   AC0200
- RefSeq:   NP_669118.1
- RefSeq:   NP_993120.1
- RefSeq:   YP_002346651.1
- ProteinModelPortal:   Q8ZFQ3
- SMR:   Q8ZFQ3
- GeneID:   1146748
- GeneID:   1174479
- GeneID:   2765572
- GenomeReviews:   AE009952_GR
- GenomeReviews:   AE017042_GR
- GenomeReviews:   AL590842_GR
- KEGG:   ype:YPO1640
- KEGG:   ypk:y1801
- KEGG:   ypm:YP_1770
- HOGENOM:   HBG754478
- OMA:   KHKLAKT
- ProtClustDB:   CLSK888905
- BioCyc:   YPES187410:Y1801-MONOMER
- BioCyc:   YPES214092:YPO1640-MONOMER
- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- TIGRFAMs:   TIGR00093

Pfam domain/function: PF00849 PseudoU_synth_2; SSF55120 PsdUridine_synth_cat_dom

EC number: NA

Molecular weight: Translated: 23543; Mature: 23543

Theoretical pI: Translated: 10.92; Mature: 10.92

Prosite motif: PS01149 PSI_RSU

Important sites: ACT_SITE 69-69

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKFSVKNHKVNRFSTKAQARQSPPAAPRRVLLFNKPFDVLPQFTDESGRTTLQGFIPFK
CCCCCCCCCCCHHHHHHHHHHCCCCCCCEEEEEECCCHHHCCCCCCCCCCEEEEECCCHH
DVYAAGRLDRDSEGLLVLTNDGKLQARLTQPAQKTGKVYFVQVEGVPDNDALTQLKRGVT
HHHHHCCCCCCCCCEEEEECCCEEEEEECCCHHHCCCEEEEEECCCCCCHHHHHHHCCCE
LKDGLTLPAGAELVAEPEWLWPRHPPIRERKTIPTSWLKITLYEGRNRQVRRMTAHVGFP
ECCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCEEEEEEECCCCC
TLRLIRFSMGNLSLGNLQPGEWKEITDV
CEEEEEECCCCEEECCCCCCCCCCCCCC
>Mature Secondary Structure
MNKFSVKNHKVNRFSTKAQARQSPPAAPRRVLLFNKPFDVLPQFTDESGRTTLQGFIPFK
CCCCCCCCCCCHHHHHHHHHHCCCCCCCEEEEEECCCHHHCCCCCCCCCCEEEEECCCHH
DVYAAGRLDRDSEGLLVLTNDGKLQARLTQPAQKTGKVYFVQVEGVPDNDALTQLKRGVT
HHHHHCCCCCCCCCEEEEECCCEEEEEECCCHHHCCCEEEEEECCCCCCHHHHHHHCCCE
LKDGLTLPAGAELVAEPEWLWPRHPPIRERKTIPTSWLKITLYEGRNRQVRRMTAHVGFP
ECCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCEEEEEEECCCCC
TLRLIRFSMGNLSLGNLQPGEWKEITDV
CEEEEEECCCCEEECCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11586360; 12142430