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The map label for this gene is sucB [H]

Identifier: 218928282

GI number: 218928282

Start: 1259844

End: 1261067

Strand: Direct

Name: sucB [H]

Synonym: YPO1114

Alternate gene names: 218928282

Gene position: 1259844-1261067 (Clockwise)

Preceding gene: 218928281

Following gene: 218928283

Centisome position: 27.07

GC content: 48.77

Gene sequence:

>1224_bases
ATGAGTAGCGTAGATATTAATGTTCCTGACCTCCCTGAGTCTGTAGCCGATGGCTCTGTAGCAACCTGGCACAAAAAACC
AGGCGACAGCGTCAAACGTGATGAAGTGCTGGTTGAGATTGAAACGGATAAAGTGATTTTGGAAGTGCCAGCCAGTCAGG
ACGGTATTTTGGATGCCATTCTGGAAGATGAAGGTGCCACAGTGACGTCACGTCAGGTGCTGGGGCGCATTCGTCCAAGT
GATAGTTCTGGTAAACCGACTGAAGAAAAAAGTCAGAGTACGGAGTCAACTCCGGCTCAACGCCAAACAGCCAGCTTGGA
AGAAGAAAGTAATGAAACCCTCAGTCCGGCTATTCGCCGCCTGATTGCTGAGCATGATCTCGATGCAACGGCAATCAAAG
GCAGCGGTGTTGGTGGCCGTATCACCCGTGAAGACGTTGATAGCCATTTAGCGAGCCGTAAATCGGCCTCTGCAGTGGTA
GCTGACGCTAAAGCGGTAGCCGCTGCTGCACCTGTATTGGCGGGGCGTAGTGAGAAACGTGTCCCGATGAGCCGTCTGCG
TAAACGTGTTGCTGAGCGTCTGTTAGAAGCGAAAAACAGCACCGCAATGTTGACCACGTTTAACGAAATTAATATGCAGC
CCATTATGGACCTGCGTAAGCAGTACGGTGAAGCTTTCGAGAAACGCCACGGTGTACGTCTGGGCTTTATGTCCTTCTAT
ATCAAGGCAGTGGTTGAAGCACTGAAACGTTATCCTGAAGTGAATGCTTCTATTGATGGCGAAGATGTTGTCTACCATAA
CTATTTCGATGTCAGTATTGCGGTTTCTACTCCACGTGGTTTGGTAACGCCAGTATTACGTGATGTAGACACGTTGAGCA
TGGCTGATATTGAGAAGAAAATAAAAGAATTGGCTGTAAAAGGTCGTGATGGCAAGCTGAAAGTTGAAGAGTTGACTGGC
GGTAACTTTACCATTACCAATGGTGGCGTGTTTGGCTCACTGATGTCGACACCGATCATTAACCCACCACAAAGCGCGAT
CCTTGGCATGCATGCCATCAAAGATCGCCCAATGGCGGTAAATGGTCAGGTTGTGATCCTGCCTATGATGTATTTGGCAC
TTTCCTACGATCACCGTTTGATCGATGGCCGCGAGTCTGTTGGCTATCTGGTGACGGTAAAAGAGATGCTGGAAGATCCT
GCTCGCTTGCTACTTGATGTATAA

Upstream 100 bases:

>100_bases
CAGTTGGGTATCTTTCCGTACACCAAAAACAACAACAAGCTCTGGTTAATGACGCGCTGAATGTTGAATAGCTAGTCGGT
TAACATATAAAGGATAGAGA

Downstream 100 bases:

>100_bases
ACTGGCTACTCGACGTATAAAAAGATAACTGACAAAGCTGGTCTTGAGGATCGGCTTTATCCAGAACACTACGGGCTTCG
GCCCGTCTCAGACCTTATAT

Product: dihydrolipoamide succinyltransferase

Products: NA

Alternate protein names: 2-oxoglutarate dehydrogenase complex component E2; OGDC-E2; Dihydrolipoamide succinyltransferase component of 2-oxoglutarate dehydrogenase complex [H]

Number of amino acids: Translated: 407; Mature: 406

Protein sequence:

>407_residues
MSSVDINVPDLPESVADGSVATWHKKPGDSVKRDEVLVEIETDKVILEVPASQDGILDAILEDEGATVTSRQVLGRIRPS
DSSGKPTEEKSQSTESTPAQRQTASLEEESNETLSPAIRRLIAEHDLDATAIKGSGVGGRITREDVDSHLASRKSASAVV
ADAKAVAAAAPVLAGRSEKRVPMSRLRKRVAERLLEAKNSTAMLTTFNEINMQPIMDLRKQYGEAFEKRHGVRLGFMSFY
IKAVVEALKRYPEVNASIDGEDVVYHNYFDVSIAVSTPRGLVTPVLRDVDTLSMADIEKKIKELAVKGRDGKLKVEELTG
GNFTITNGGVFGSLMSTPIINPPQSAILGMHAIKDRPMAVNGQVVILPMMYLALSYDHRLIDGRESVGYLVTVKEMLEDP
ARLLLDV

Sequences:

>Translated_407_residues
MSSVDINVPDLPESVADGSVATWHKKPGDSVKRDEVLVEIETDKVILEVPASQDGILDAILEDEGATVTSRQVLGRIRPS
DSSGKPTEEKSQSTESTPAQRQTASLEEESNETLSPAIRRLIAEHDLDATAIKGSGVGGRITREDVDSHLASRKSASAVV
ADAKAVAAAAPVLAGRSEKRVPMSRLRKRVAERLLEAKNSTAMLTTFNEINMQPIMDLRKQYGEAFEKRHGVRLGFMSFY
IKAVVEALKRYPEVNASIDGEDVVYHNYFDVSIAVSTPRGLVTPVLRDVDTLSMADIEKKIKELAVKGRDGKLKVEELTG
GNFTITNGGVFGSLMSTPIINPPQSAILGMHAIKDRPMAVNGQVVILPMMYLALSYDHRLIDGRESVGYLVTVKEMLEDP
ARLLLDV
>Mature_406_residues
SSVDINVPDLPESVADGSVATWHKKPGDSVKRDEVLVEIETDKVILEVPASQDGILDAILEDEGATVTSRQVLGRIRPSD
SSGKPTEEKSQSTESTPAQRQTASLEEESNETLSPAIRRLIAEHDLDATAIKGSGVGGRITREDVDSHLASRKSASAVVA
DAKAVAAAAPVLAGRSEKRVPMSRLRKRVAERLLEAKNSTAMLTTFNEINMQPIMDLRKQYGEAFEKRHGVRLGFMSFYI
KAVVEALKRYPEVNASIDGEDVVYHNYFDVSIAVSTPRGLVTPVLRDVDTLSMADIEKKIKELAVKGRDGKLKVEELTGG
NFTITNGGVFGSLMSTPIINPPQSAILGMHAIKDRPMAVNGQVVILPMMYLALSYDHRLIDGRESVGYLVTVKEMLEDPA
RLLLDV

Specific function: The 2-oxoglutarate dehydrogenase complex catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO(2). It contains multiple copies of three enzymatic components:2- oxoglutarate dehydrogenase (E1), dihydrolipoamide succinyltransferase (E2)

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI19923748, Length=234, Percent_Identity=58.1196581196581, Blast_Score=290, Evalue=1e-78,
Organism=Homo sapiens, GI31711992, Length=428, Percent_Identity=29.6728971962617, Blast_Score=185, Evalue=6e-47,
Organism=Homo sapiens, GI203098753, Length=451, Percent_Identity=28.159645232816, Blast_Score=162, Evalue=5e-40,
Organism=Homo sapiens, GI203098816, Length=451, Percent_Identity=28.159645232816, Blast_Score=161, Evalue=9e-40,
Organism=Homo sapiens, GI110671329, Length=430, Percent_Identity=27.6744186046512, Blast_Score=161, Evalue=1e-39,
Organism=Homo sapiens, GI260898739, Length=168, Percent_Identity=34.5238095238095, Blast_Score=103, Evalue=2e-22,
Organism=Escherichia coli, GI1786946, Length=407, Percent_Identity=84.2751842751843, Blast_Score=688, Evalue=0.0,
Organism=Escherichia coli, GI1786305, Length=427, Percent_Identity=28.3372365339578, Blast_Score=155, Evalue=3e-39,
Organism=Caenorhabditis elegans, GI25146366, Length=408, Percent_Identity=41.6666666666667, Blast_Score=322, Evalue=2e-88,
Organism=Caenorhabditis elegans, GI17560088, Length=440, Percent_Identity=31.1363636363636, Blast_Score=177, Evalue=1e-44,
Organism=Caenorhabditis elegans, GI17537937, Length=419, Percent_Identity=25.0596658711217, Blast_Score=155, Evalue=4e-38,
Organism=Caenorhabditis elegans, GI17538894, Length=325, Percent_Identity=29.5384615384615, Blast_Score=132, Evalue=2e-31,
Organism=Saccharomyces cerevisiae, GI6320352, Length=406, Percent_Identity=43.1034482758621, Blast_Score=330, Evalue=3e-91,
Organism=Saccharomyces cerevisiae, GI6324258, Length=455, Percent_Identity=27.4725274725275, Blast_Score=156, Evalue=6e-39,
Organism=Drosophila melanogaster, GI24645909, Length=230, Percent_Identity=57.8260869565217, Blast_Score=287, Evalue=1e-77,
Organism=Drosophila melanogaster, GI18859875, Length=425, Percent_Identity=28.7058823529412, Blast_Score=159, Evalue=4e-39,
Organism=Drosophila melanogaster, GI20129315, Length=231, Percent_Identity=33.3333333333333, Blast_Score=131, Evalue=7e-31,
Organism=Drosophila melanogaster, GI24582497, Length=231, Percent_Identity=33.3333333333333, Blast_Score=131, Evalue=8e-31,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3096 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053
- InterPro:   IPR006255 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.61 [H]

Molecular weight: Translated: 44314; Mature: 44183

Theoretical pI: Translated: 5.38; Mature: 5.38

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSVDINVPDLPESVADGSVATWHKKPGDSVKRDEVLVEIETDKVILEVPASQDGILDAI
CCCCCCCCCCCCHHHCCCCEEECCCCCCCCCCCCCEEEEEECCEEEEECCCCCCCHHHHH
LEDEGATVTSRQVLGRIRPSDSSGKPTEEKSQSTESTPAQRQTASLEEESNETLSPAIRR
HCCCCCEEEHHHHHHCCCCCCCCCCCCHHHHCCCCCCCHHHHHHHHHHHCCCHHHHHHHH
LIAEHDLDATAIKGSGVGGRITREDVDSHLASRKSASAVVADAKAVAAAAPVLAGRSEKR
HHHHCCCCCEEEECCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCC
VPMSRLRKRVAERLLEAKNSTAMLTTFNEINMQPIMDLRKQYGEAFEKRHGVRLGFMSFY
CCHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCEEHHHHHH
IKAVVEALKRYPEVNASIDGEDVVYHNYFDVSIAVSTPRGLVTPVLRDVDTLSMADIEKK
HHHHHHHHHHCCCCCCCCCCCCEEEEEEEEEEEEEECCCHHHHHHHHCCHHHHHHHHHHH
IKELAVKGRDGKLKVEELTGGNFTITNGGVFGSLMSTPIINPPQSAILGMHAIKDRPMAV
HHHHHHCCCCCCEEEEEECCCCEEEECCCHHHHHHHCCCCCCCHHHHHHHHHHCCCCEEE
NGQVVILPMMYLALSYDHRLIDGRESVGYLVTVKEMLEDPARLLLDV
CCCEEHHHHHHHHHHCCCCCCCCCCCCCCEEEHHHHHCCHHHHHCCC
>Mature Secondary Structure 
SSVDINVPDLPESVADGSVATWHKKPGDSVKRDEVLVEIETDKVILEVPASQDGILDAI
CCCCCCCCCCCHHHCCCCEEECCCCCCCCCCCCCEEEEEECCEEEEECCCCCCCHHHHH
LEDEGATVTSRQVLGRIRPSDSSGKPTEEKSQSTESTPAQRQTASLEEESNETLSPAIRR
HCCCCCEEEHHHHHHCCCCCCCCCCCCHHHHCCCCCCCHHHHHHHHHHHCCCHHHHHHHH
LIAEHDLDATAIKGSGVGGRITREDVDSHLASRKSASAVVADAKAVAAAAPVLAGRSEKR
HHHHCCCCCEEEECCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCC
VPMSRLRKRVAERLLEAKNSTAMLTTFNEINMQPIMDLRKQYGEAFEKRHGVRLGFMSFY
CCHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCEEHHHHHH
IKAVVEALKRYPEVNASIDGEDVVYHNYFDVSIAVSTPRGLVTPVLRDVDTLSMADIEKK
HHHHHHHHHHCCCCCCCCCCCCEEEEEEEEEEEEEECCCHHHHHHHHCCHHHHHHHHHHH
IKELAVKGRDGKLKVEELTGGNFTITNGGVFGSLMSTPIINPPQSAILGMHAIKDRPMAV
HHHHHHCCCCCCEEEEEECCCCEEEECCCHHHHHHHCCCCCCCHHHHHHHHHHCCCCEEE
NGQVVILPMMYLALSYDHRLIDGRESVGYLVTVKEMLEDPARLLLDV
CCCEEHHHHHHHHHHCCCCCCCCCCCCCCEEEHHHHHCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]