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The map label for this gene is gltA [H]

Identifier: 218928276

GI number: 218928276

Start: 1251398

End: 1252678

Strand: Reverse

Name: gltA [H]

Synonym: YPO1108

Alternate gene names: 218928276

Gene position: 1252678-1251398 (Counterclockwise)

Preceding gene: 218928297

Following gene: 218928274

Centisome position: 26.92

GC content: 48.56

Gene sequence:

>1281_bases
ATGGCTGACAAAAAAGCGACGCTGAATTTAGGCGAAGCTGCGATCGAACTGGGTGTCCTATCCCCAACCCTCGGCACTGA
CGTGATCGACGTCCGCACCTTAGGTTCTAAAGGTTATTTTACCTTTGACCCCGGTTTTACCTCTACCGCATCCTGCGAAT
CAAAAATCACCTATATTGATGGTGATCAGGGTATTTTGCTGCACCGTGGCTTCCCGATTGCCCAGTTGGCAAAAGATTCT
ACTTATCTGGAAGTTTGTTACATTCTGCTGTACGGCGAAACACCTACGCCAGAAGAGTACAAAACGTTCAAAACCACAGT
GACCCGACATACCATGATCCACGAGCAGATCACCCGTCTGTTCCATGGTTTCCGCCGCGATTCACATCCGATGGCCGTGC
TATGTGGGGTTACTGGTGCGCTAGCCGCCTTCTACCATGATGCTCTGGATGTTAATAACGAGCGCCATCGTGAAATTACC
GCCTTCCGTCTGTTGTCTAAAATGCCGACTGTCGCAGCGATGTGTTATAAATATTCACTGGGTCAACCGTTTGTTTATCC
ACGTAACGATCTGTCCTACGCGGGTAACTTCCTACACATGATGTTCTCTACGCCATGTGAGAAATATGAAGTTAACCCAG
TACTGGAACGCGCTATGGATCGTATTCTCATCCTGCACGCAGATCACGAACAGAACGCGTCAACATCGACCGTCCGTACT
GCCGGCTCTTCTGGCGCTAACCCGTTTGCCTGTATCGCCGCGGGGATCGCATCCCTGTGGGGCCCTGCGCACGGTGGTGC
CAATGAAGCCTGCCTGAAGATGTTGGAAGAGATTAAAACTGTTGAGCATATCCCTGAATTTATCCGCCGCGCGAAAGATA
AGAATGACTCATTCCGTCTGATGGGTTTTGGTCACCGTGTCTATAAAAACTATGACCCTCGTGCCACCGTCATGCGTGAA
ACCTGTCATGAAGTGTTAGAAGAGCTGAAGCTGAATAACAGCTTGTTAGAAGTGGCGATGGAGTTGGAAAACATCGCACT
GAACGATCCTTACTTCATTGAGAAAAAACTGTATCCAAACGTGGACTTCTATTCAGGTATTATCCTGAAAGCCATGGGGA
TTCCATCGTCAATGTTTACGGTGATATTCGCCATTGCCCGAACCATCGGTTGGATTGCTCACTGGAACGAAATGCATAAT
GACGGCATCAAGATTGCCCGTCCGCGCCAGCTTTATACTGGCTATACAGAACGTGATTTCCAGAGCCAATTGAAAAAATA
A

Upstream 100 bases:

>100_bases
GATGAATAAAACAGAGCAGTGCTAATAAGGTGATATCCCCGGGCATCTAAGATGTTTCCGTTGTTAGGGGTTTTAAAATA
AGCACTTAAGGAGACTGTAA

Downstream 100 bases:

>100_bases
GCAGGTACGCGTTACCGCGTAAAATTAGAGCAAAGTCCCGGCTAGGAGGCCAGGACTTTGTTAGCAATTTGAGGCACCGT
TGAGGTGCCTTTTAACGTTA

Product: type II citrate synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 426; Mature: 425

Protein sequence:

>426_residues
MADKKATLNLGEAAIELGVLSPTLGTDVIDVRTLGSKGYFTFDPGFTSTASCESKITYIDGDQGILLHRGFPIAQLAKDS
TYLEVCYILLYGETPTPEEYKTFKTTVTRHTMIHEQITRLFHGFRRDSHPMAVLCGVTGALAAFYHDALDVNNERHREIT
AFRLLSKMPTVAAMCYKYSLGQPFVYPRNDLSYAGNFLHMMFSTPCEKYEVNPVLERAMDRILILHADHEQNASTSTVRT
AGSSGANPFACIAAGIASLWGPAHGGANEACLKMLEEIKTVEHIPEFIRRAKDKNDSFRLMGFGHRVYKNYDPRATVMRE
TCHEVLEELKLNNSLLEVAMELENIALNDPYFIEKKLYPNVDFYSGIILKAMGIPSSMFTVIFAIARTIGWIAHWNEMHN
DGIKIARPRQLYTGYTERDFQSQLKK

Sequences:

>Translated_426_residues
MADKKATLNLGEAAIELGVLSPTLGTDVIDVRTLGSKGYFTFDPGFTSTASCESKITYIDGDQGILLHRGFPIAQLAKDS
TYLEVCYILLYGETPTPEEYKTFKTTVTRHTMIHEQITRLFHGFRRDSHPMAVLCGVTGALAAFYHDALDVNNERHREIT
AFRLLSKMPTVAAMCYKYSLGQPFVYPRNDLSYAGNFLHMMFSTPCEKYEVNPVLERAMDRILILHADHEQNASTSTVRT
AGSSGANPFACIAAGIASLWGPAHGGANEACLKMLEEIKTVEHIPEFIRRAKDKNDSFRLMGFGHRVYKNYDPRATVMRE
TCHEVLEELKLNNSLLEVAMELENIALNDPYFIEKKLYPNVDFYSGIILKAMGIPSSMFTVIFAIARTIGWIAHWNEMHN
DGIKIARPRQLYTGYTERDFQSQLKK
>Mature_425_residues
ADKKATLNLGEAAIELGVLSPTLGTDVIDVRTLGSKGYFTFDPGFTSTASCESKITYIDGDQGILLHRGFPIAQLAKDST
YLEVCYILLYGETPTPEEYKTFKTTVTRHTMIHEQITRLFHGFRRDSHPMAVLCGVTGALAAFYHDALDVNNERHREITA
FRLLSKMPTVAAMCYKYSLGQPFVYPRNDLSYAGNFLHMMFSTPCEKYEVNPVLERAMDRILILHADHEQNASTSTVRTA
GSSGANPFACIAAGIASLWGPAHGGANEACLKMLEEIKTVEHIPEFIRRAKDKNDSFRLMGFGHRVYKNYDPRATVMRET
CHEVLEELKLNNSLLEVAMELENIALNDPYFIEKKLYPNVDFYSGIILKAMGIPSSMFTVIFAIARTIGWIAHWNEMHND
GIKIARPRQLYTGYTERDFQSQLKK

Specific function: Tricarboxylic acid cycle. [C]

COG id: COG0372

COG function: function code C; Citrate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the citrate synthase family [H]

Homologues:

Organism=Homo sapiens, GI38327625, Length=390, Percent_Identity=25.6410256410256, Blast_Score=108, Evalue=9e-24,
Organism=Escherichia coli, GI1786939, Length=427, Percent_Identity=86.6510538641686, Blast_Score=789, Evalue=0.0,
Organism=Escherichia coli, GI1786527, Length=366, Percent_Identity=32.5136612021858, Blast_Score=169, Evalue=3e-43,
Organism=Caenorhabditis elegans, GI17555174, Length=399, Percent_Identity=25.062656641604, Blast_Score=110, Evalue=1e-24,
Organism=Saccharomyces cerevisiae, GI6324328, Length=370, Percent_Identity=28.1081081081081, Blast_Score=114, Evalue=3e-26,
Organism=Saccharomyces cerevisiae, GI6319850, Length=392, Percent_Identity=27.5510204081633, Blast_Score=110, Evalue=4e-25,
Organism=Saccharomyces cerevisiae, GI6325257, Length=362, Percent_Identity=25.1381215469613, Blast_Score=85, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24640126, Length=402, Percent_Identity=25.6218905472637, Blast_Score=115, Evalue=8e-26,
Organism=Drosophila melanogaster, GI24640124, Length=402, Percent_Identity=25.6218905472637, Blast_Score=114, Evalue=9e-26,
Organism=Drosophila melanogaster, GI21356863, Length=393, Percent_Identity=26.2086513994911, Blast_Score=100, Evalue=2e-21,

Paralogues:

None

Copy number: 624 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016142
- InterPro:   IPR016143
- InterPro:   IPR002020
- InterPro:   IPR016141
- InterPro:   IPR019810
- InterPro:   IPR010953 [H]

Pfam domain/function: PF00285 Citrate_synt [H]

EC number: =2.3.3.1 [H]

Molecular weight: Translated: 47980; Mature: 47848

Theoretical pI: Translated: 6.95; Mature: 6.95

Prosite motif: PS00480 CITRATE_SYNTHASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADKKATLNLGEAAIELGVLSPTLGTDVIDVRTLGSKGYFTFDPGFTSTASCESKITYID
CCCCCCCCCCCHHHHHHCCCCCCCCCCEEEEEECCCCCEEEECCCCCCCCCCCCEEEEEE
GDQGILLHRGFPIAQLAKDSTYLEVCYILLYGETPTPEEYKTFKTTVTRHTMIHEQITRL
CCCCEEEECCCCHHHHHCCCHHHHEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHH
FHGFRRDSHPMAVLCGVTGALAAFYHDALDVNNERHREITAFRLLSKMPTVAAMCYKYSL
HHHHCCCCCCEEEEHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCHHHHHHHHHHHC
GQPFVYPRNDLSYAGNFLHMMFSTPCEKYEVNPVLERAMDRILILHADHEQNASTSTVRT
CCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHEEEEECCCCCCCCCHHHEE
AGSSGANPFACIAAGIASLWGPAHGGANEACLKMLEEIKTVEHIPEFIRRAKDKNDSFRL
CCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEE
MGFGHRVYKNYDPRATVMRETCHEVLEELKLNNSLLEVAMELENIALNDPYFIEKKLYPN
EECCHHHHCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCEEEEHCCCCC
VDFYSGIILKAMGIPSSMFTVIFAIARTIGWIAHWNEMHNDGIKIARPRQLYTGYTERDF
CCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCHHHHCCCCHHHH
QSQLKK
HHHHCC
>Mature Secondary Structure 
ADKKATLNLGEAAIELGVLSPTLGTDVIDVRTLGSKGYFTFDPGFTSTASCESKITYID
CCCCCCCCCCHHHHHHCCCCCCCCCCEEEEEECCCCCEEEECCCCCCCCCCCCEEEEEE
GDQGILLHRGFPIAQLAKDSTYLEVCYILLYGETPTPEEYKTFKTTVTRHTMIHEQITRL
CCCCEEEECCCCHHHHHCCCHHHHEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHH
FHGFRRDSHPMAVLCGVTGALAAFYHDALDVNNERHREITAFRLLSKMPTVAAMCYKYSL
HHHHCCCCCCEEEEHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCHHHHHHHHHHHC
GQPFVYPRNDLSYAGNFLHMMFSTPCEKYEVNPVLERAMDRILILHADHEQNASTSTVRT
CCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHEEEEECCCCCCCCCHHHEE
AGSSGANPFACIAAGIASLWGPAHGGANEACLKMLEEIKTVEHIPEFIRRAKDKNDSFRL
CCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEE
MGFGHRVYKNYDPRATVMRETCHEVLEELKLNNSLLEVAMELENIALNDPYFIEKKLYPN
EECCHHHHCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCEEEEHCCCCC
VDFYSGIILKAMGIPSSMFTVIFAIARTIGWIAHWNEMHNDGIKIARPRQLYTGYTERDF
CCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCHHHHCCCCHHHH
QSQLKK
HHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]