Click here to switch to the map view.

The map label for this gene is ppnK

Identifier: 218928274

GI number: 218928274

Start: 1249707

End: 1250588

Strand: Reverse

Name: ppnK

Synonym: YPO1106

Alternate gene names: 218928274

Gene position: 1250588-1249707 (Counterclockwise)

Preceding gene: 218928276

Following gene: 218928273

Centisome position: 26.87

GC content: 47.05

Gene sequence:

>882_bases
ATGAATAATAGAAGATTCGATTGTATTGGTATTGTCGGTCACCCGCGACACCCGGCTGCACTTGCCACCCATGAAATACT
CTACCACTGGCTAAAAGCCAGAGGCTACGCCGTTATGGTTGAACAACAGATTGCTCATGACCTCAATTTAACCGATGCCA
TAACAGGGAGTTTGGCAGATATTGGCCAAAAAGCAGATTTGGCTGTTGTTGTCGGCGGTGATGGCAACATGCTCGGTGCT
GCACGGGTACTTGCCCGTTATGATATAAAAGTGATTGGTGTCAACCGAGGTAATCTGGGTTTCTTGACCGATTTAGACCC
GGATAATGCTCTGCAGCAGCTTTCAGACGTTTTGGAAGGCGAGTACCTGAGTGAACAACGCTTCCTGCTAGAAACGCATG
TACGACGTACAAATCAACAAAGCCGTATCAGTACCGCAATCAATGAAGTGGTTTTGCATCCAGGCAAAGTCGCCCATATG
ATCGAATTCGAAGTGTATATTGATGACCGCTTCGCCTTTTCTCAACGCTCTGATGGCTTGATCATCGCAACCCCGACCGG
TTCTACCGCCTATTCACTTTCCGCAGGCGGCCCAATCTTGACACCAACTCTGGATGCCATCGTATTGGTACCAATGTTTC
CACACACTCTTACTGCGCGCCCATTGGTTATCAGCAGCAGTAGCACCATCCGACTGAAGTTTTCGCACATTACCAGCGAC
CTGGAAATCAGTTGCGATAGCCAGATAGCCCTACCCATTCAGGAAGGGGAAGAGGTTTTAATCCGGCGCAGTGATTTTCA
CCTCAATCTTATTCATCCAAAGGACTATAGCTATTTCAATACGTTAAGCACCAAATTGGGTTGGTCAAAAAAATTATTCT
AA

Upstream 100 bases:

>100_bases
AATCTCGCTACTTGGCTTATTATGGGGATCAAAACCGGGGATTCAAGGGAACCGGTCATAATGTGGGGTAAAAGACACTC
CATTAAGGAAAATCACGGCA

Downstream 100 bases:

>100_bases
AAATCACCTCAGCTACTTTACTGTATATAAAACCAGTCTATACTGTGTTCAAATACAGACATGTGTTTATGTACAGGGGG
TTTACCATGCTGGTCCAATT

Product: inorganic polyphosphate/ATP-NAD kinase

Products: NA

Alternate protein names: Poly(P)/ATP NAD kinase

Number of amino acids: Translated: 293; Mature: 293

Protein sequence:

>293_residues
MNNRRFDCIGIVGHPRHPAALATHEILYHWLKARGYAVMVEQQIAHDLNLTDAITGSLADIGQKADLAVVVGGDGNMLGA
ARVLARYDIKVIGVNRGNLGFLTDLDPDNALQQLSDVLEGEYLSEQRFLLETHVRRTNQQSRISTAINEVVLHPGKVAHM
IEFEVYIDDRFAFSQRSDGLIIATPTGSTAYSLSAGGPILTPTLDAIVLVPMFPHTLTARPLVISSSSTIRLKFSHITSD
LEISCDSQIALPIQEGEEVLIRRSDFHLNLIHPKDYSYFNTLSTKLGWSKKLF

Sequences:

>Translated_293_residues
MNNRRFDCIGIVGHPRHPAALATHEILYHWLKARGYAVMVEQQIAHDLNLTDAITGSLADIGQKADLAVVVGGDGNMLGA
ARVLARYDIKVIGVNRGNLGFLTDLDPDNALQQLSDVLEGEYLSEQRFLLETHVRRTNQQSRISTAINEVVLHPGKVAHM
IEFEVYIDDRFAFSQRSDGLIIATPTGSTAYSLSAGGPILTPTLDAIVLVPMFPHTLTARPLVISSSSTIRLKFSHITSD
LEISCDSQIALPIQEGEEVLIRRSDFHLNLIHPKDYSYFNTLSTKLGWSKKLF
>Mature_293_residues
MNNRRFDCIGIVGHPRHPAALATHEILYHWLKARGYAVMVEQQIAHDLNLTDAITGSLADIGQKADLAVVVGGDGNMLGA
ARVLARYDIKVIGVNRGNLGFLTDLDPDNALQQLSDVLEGEYLSEQRFLLETHVRRTNQQSRISTAINEVVLHPGKVAHM
IEFEVYIDDRFAFSQRSDGLIIATPTGSTAYSLSAGGPILTPTLDAIVLVPMFPHTLTARPLVISSSSTIRLKFSHITSD
LEISCDSQIALPIQEGEEVLIRRSDFHLNLIHPKDYSYFNTLSTKLGWSKKLF

Specific function: Catalyzes the phosphorylation of NAD to NADP. Utilizes ATP and other nucleoside triphosphates as well as inorganic polyphosphate as a source of phosphorus

COG id: COG0061

COG function: function code G; Predicted sugar kinase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NAD kinase family

Homologues:

Organism=Homo sapiens, GI55743112, Length=259, Percent_Identity=27.027027027027, Blast_Score=86, Evalue=4e-17,
Organism=Escherichia coli, GI1788968, Length=293, Percent_Identity=82.9351535836177, Blast_Score=504, Evalue=1e-144,
Organism=Saccharomyces cerevisiae, GI6325068, Length=215, Percent_Identity=33.953488372093, Blast_Score=127, Evalue=3e-30,
Organism=Saccharomyces cerevisiae, GI6320794, Length=243, Percent_Identity=29.2181069958848, Blast_Score=119, Evalue=6e-28,
Organism=Saccharomyces cerevisiae, GI6322509, Length=240, Percent_Identity=30, Blast_Score=117, Evalue=3e-27,
Organism=Drosophila melanogaster, GI28573832, Length=305, Percent_Identity=27.5409836065574, Blast_Score=95, Evalue=6e-20,
Organism=Drosophila melanogaster, GI28573830, Length=307, Percent_Identity=27.3615635179153, Blast_Score=95, Evalue=7e-20,
Organism=Drosophila melanogaster, GI28573826, Length=307, Percent_Identity=27.3615635179153, Blast_Score=94, Evalue=7e-20,
Organism=Drosophila melanogaster, GI28573828, Length=307, Percent_Identity=27.3615635179153, Blast_Score=94, Evalue=8e-20,
Organism=Drosophila melanogaster, GI161077047, Length=305, Percent_Identity=27.5409836065574, Blast_Score=94, Evalue=8e-20,
Organism=Drosophila melanogaster, GI20129957, Length=276, Percent_Identity=24.6376811594203, Blast_Score=84, Evalue=8e-17,
Organism=Drosophila melanogaster, GI24653422, Length=276, Percent_Identity=24.6376811594203, Blast_Score=84, Evalue=8e-17,
Organism=Drosophila melanogaster, GI281363323, Length=276, Percent_Identity=24.6376811594203, Blast_Score=84, Evalue=1e-16,
Organism=Drosophila melanogaster, GI281363321, Length=276, Percent_Identity=24.6376811594203, Blast_Score=84, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24653424, Length=276, Percent_Identity=24.6376811594203, Blast_Score=84, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PPNK_YERPE (Q8ZH09)

Other databases:

- EMBL:   AL590842
- EMBL:   AE009952
- EMBL:   AE017042
- PIR:   AB0136
- RefSeq:   NP_670373.1
- RefSeq:   YP_002346149.1
- ProteinModelPortal:   Q8ZH09
- SMR:   Q8ZH09
- IntAct:   Q8ZH09
- GeneID:   1148021
- GeneID:   1173954
- GenomeReviews:   AE009952_GR
- GenomeReviews:   AE017042_GR
- GenomeReviews:   AL590842_GR
- KEGG:   ype:YPO1106
- KEGG:   ypk:y3074
- KEGG:   ypm:YP_1050
- HOGENOM:   HBG713904
- OMA:   DGTMLNA
- ProtClustDB:   PRK03378
- BioCyc:   YPES187410:Y3074-MONOMER
- BioCyc:   YPES214092:YPO1106-MONOMER
- BRENDA:   2.7.1.23
- GO:   GO:0005737
- HAMAP:   MF_00361
- InterPro:   IPR016064
- InterPro:   IPR017438
- InterPro:   IPR017437
- InterPro:   IPR002504
- Gene3D:   G3DSA:2.60.200.30
- Gene3D:   G3DSA:3.40.50.10330
- PANTHER:   PTHR20275

Pfam domain/function: PF01513 NAD_kinase; SSF111331 ATP-NAD_kinase_PpnK-typ

EC number: =2.7.1.23

Molecular weight: Translated: 32398; Mature: 32398

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNRRFDCIGIVGHPRHPAALATHEILYHWLKARGYAVMVEQQIAHDLNLTDAITGSLAD
CCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHCCEEEEEEHHHHCCCCCHHHHCCCHHH
IGQKADLAVVVGGDGNMLGAARVLARYDIKVIGVNRGNLGFLTDLDPDNALQQLSDVLEG
CCCCCCEEEEECCCCCCHHHHHHHHHCCEEEEEECCCCEEEEECCCCHHHHHHHHHHHCC
EYLSEQRFLLETHVRRTNQQSRISTAINEVVLHPGKVAHMIEFEVYIDDRFAFSQRSDGL
CHHCHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCEEEEEEEEEEEECEEEECCCCCCE
IIATPTGSTAYSLSAGGPILTPTLDAIVLVPMFPHTLTARPLVISSSSTIRLKFSHITSD
EEEECCCCCEEEECCCCCEECCCCCEEEEEECCCCCCCCCEEEEECCCEEEEEEEECCCC
LEISCDSQIALPIQEGEEVLIRRSDFHLNLIHPKDYSYFNTLSTKLGWSKKLF
EEEECCCEEEEECCCCCEEEEEECCCEEEEECCCCCCHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MNNRRFDCIGIVGHPRHPAALATHEILYHWLKARGYAVMVEQQIAHDLNLTDAITGSLAD
CCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHCCEEEEEEHHHHCCCCCHHHHCCCHHH
IGQKADLAVVVGGDGNMLGAARVLARYDIKVIGVNRGNLGFLTDLDPDNALQQLSDVLEG
CCCCCCEEEEECCCCCCHHHHHHHHHCCEEEEEECCCCEEEEECCCCHHHHHHHHHHHCC
EYLSEQRFLLETHVRRTNQQSRISTAINEVVLHPGKVAHMIEFEVYIDDRFAFSQRSDGL
CHHCHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCEEEEEEEEEEEECEEEECCCCCCE
IIATPTGSTAYSLSAGGPILTPTLDAIVLVPMFPHTLTARPLVISSSSTIRLKFSHITSD
EEEECCCCCEEEECCCCCEECCCCCEEEEEECCCCCCCCCEEEEECCCEEEEEEEECCCC
LEISCDSQIALPIQEGEEVLIRRSDFHLNLIHPKDYSYFNTLSTKLGWSKKLF
EEEECCCEEEEECCCCCEEEEEECCCEEEEECCCCCCHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11586360; 12142430