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The map label for this gene is pucI [H]

Identifier: 218928252

GI number: 218928252

Start: 1230242

End: 1231726

Strand: Reverse

Name: pucI [H]

Synonym: YPO1083

Alternate gene names: 218928252

Gene position: 1231726-1230242 (Counterclockwise)

Preceding gene: 218928253

Following gene: 218928250

Centisome position: 26.47

GC content: 48.69

Gene sequence:

>1485_bases
ATGCCAAATAATGAAAAAATCTCGGCTAGCAGCATTAACCCCCACTACAGTCCCAAACTCACTAATGAAGATCTGGCCCC
AACTCGTAAGCAGAACTGGGGCTGGTACAATATCTTTTCATTTTGGATGACGGATGTGCACAGCATGGGGGGTTATGTGG
TCGCCGCCAGCTTCTTTGCTCTCGGGCTATCCAGTTGGCAAGTGCTGTTATCGCTATTAGTCGGTATTTGCATTGTACAA
ATTTGCGCCAATCTGGTGGCCAAACCAAGCCAGATAGCCGGTGTGCCCTATGCCGTTATCTGTCGACAATCTTTTGGGGT
ATTTGGAGCAAATATCCCAGCCGTTATCCGTGGGCTGATCGCCGTTGCCTGGTATGGCATTCAAACCTATCTGGCGGGTA
ATGCTTTTATGCTCGTGATGCTGAAATTCTTCCCCAGCCTGATCCCCCTGGCTCAGAGCCAATGGCTAGGGTTATCCACC
CTCGGCTGGATCTGTTTCTCCATTATGTGGGTACTGCAGGCGATGGTGTTCTGGCATGGGATGAATGCGATTAAGCGCTT
TATTGATTTTGCCGGGCCGGCAGTCTATGTCGTGATGCTGTCATTGGCGTTATGGATTGTCTATCAAACCGGCTGGAGTA
ACATTTCATTCACCCTCGCCAGTAAAATCTTAAGCCCATCAGAACAGGTCTGGCAGATGCTGACGGCAACCGCGCTGGTC
GTCTCTTATTTTGCCGGGCCATTACTGAACTTTGGTGATTTCTCCCGCTATGGCAAAAGTATGCAGGAAATCCGCCGAGG
CAACCGATGGGGGCTACCCTTTAACTTCCTCCTGTTCTCTCTCGTCACCGTGGTGATTGTCTCCGGCTCACAGTCGCTGT
TCGGTGAAATGATTACCGACCCTATCGAGACCGTTACCCGCGTCGGCAATGGCGTGGCTATCGCTCTAGGTCTACTGACC
ATGATTATCGCCACTATCGGGATTAATATCGTCGCAAACTTTGTTTCACCCGCCTTTGATTTTTCTAACTGTTCACCACA
AAGAATCAACTTTCGTACTGGTGGGATGATAGCTGCGGTAGGTTCAGTTTTGCTTACCCCATGGAACCTGTTCCAATCAC
CAATCATTATCCACTACACCCTGGACGTGCTGGGTGCCTTTATGGGGCCGCTATTTGGTATTTTAATCGCTGATTTTTAT
CTGATTAAACGCGGCCAATTATATGTAGATGATCTGTTTAATGCTTCATCATCAGGACGTTATTGGTATCGCGGAGGTTT
TAATCCGAAAGCGATTATGGCGTTACTGCCCGCAGTGGCCATTGGCTTGATGATCATTTTCATCCCCAACCTACAGCAGA
TCTCCAGTTTCAGTTGGTTTATCGGGGCGTTTCTTGGCGGGAGTATTTATCGCTTTATTGCACGCAATGACCCATTGCCG
AACAATGCGGCTCTGTTTATACGCCCTGAAATAGGCAAAGATTGA

Upstream 100 bases:

>100_bases
CAAATATCTCTAGCCCTAACTCTGTAAATATTTCTCATGTGAGGTTCGACAAACCTTGTCTCACATCATCTGAATGTTGC
CTTTGGAAGGAACGCGCTTT

Downstream 100 bases:

>100_bases
GTTAAGCAGAAACGACAAAGCCCTGAGTTATTACTTAACTCAGGGCTTCTAAATTTTGGCGGTGCGGACGGGACTCGAAC
CCGCGACCCCCGGCGTGACA

Product: putative permease

Products: NA

Alternate protein names: Allantoin transport protein [H]

Number of amino acids: Translated: 494; Mature: 493

Protein sequence:

>494_residues
MPNNEKISASSINPHYSPKLTNEDLAPTRKQNWGWYNIFSFWMTDVHSMGGYVVAASFFALGLSSWQVLLSLLVGICIVQ
ICANLVAKPSQIAGVPYAVICRQSFGVFGANIPAVIRGLIAVAWYGIQTYLAGNAFMLVMLKFFPSLIPLAQSQWLGLST
LGWICFSIMWVLQAMVFWHGMNAIKRFIDFAGPAVYVVMLSLALWIVYQTGWSNISFTLASKILSPSEQVWQMLTATALV
VSYFAGPLLNFGDFSRYGKSMQEIRRGNRWGLPFNFLLFSLVTVVIVSGSQSLFGEMITDPIETVTRVGNGVAIALGLLT
MIIATIGINIVANFVSPAFDFSNCSPQRINFRTGGMIAAVGSVLLTPWNLFQSPIIIHYTLDVLGAFMGPLFGILIADFY
LIKRGQLYVDDLFNASSSGRYWYRGGFNPKAIMALLPAVAIGLMIIFIPNLQQISSFSWFIGAFLGGSIYRFIARNDPLP
NNAALFIRPEIGKD

Sequences:

>Translated_494_residues
MPNNEKISASSINPHYSPKLTNEDLAPTRKQNWGWYNIFSFWMTDVHSMGGYVVAASFFALGLSSWQVLLSLLVGICIVQ
ICANLVAKPSQIAGVPYAVICRQSFGVFGANIPAVIRGLIAVAWYGIQTYLAGNAFMLVMLKFFPSLIPLAQSQWLGLST
LGWICFSIMWVLQAMVFWHGMNAIKRFIDFAGPAVYVVMLSLALWIVYQTGWSNISFTLASKILSPSEQVWQMLTATALV
VSYFAGPLLNFGDFSRYGKSMQEIRRGNRWGLPFNFLLFSLVTVVIVSGSQSLFGEMITDPIETVTRVGNGVAIALGLLT
MIIATIGINIVANFVSPAFDFSNCSPQRINFRTGGMIAAVGSVLLTPWNLFQSPIIIHYTLDVLGAFMGPLFGILIADFY
LIKRGQLYVDDLFNASSSGRYWYRGGFNPKAIMALLPAVAIGLMIIFIPNLQQISSFSWFIGAFLGGSIYRFIARNDPLP
NNAALFIRPEIGKD
>Mature_493_residues
PNNEKISASSINPHYSPKLTNEDLAPTRKQNWGWYNIFSFWMTDVHSMGGYVVAASFFALGLSSWQVLLSLLVGICIVQI
CANLVAKPSQIAGVPYAVICRQSFGVFGANIPAVIRGLIAVAWYGIQTYLAGNAFMLVMLKFFPSLIPLAQSQWLGLSTL
GWICFSIMWVLQAMVFWHGMNAIKRFIDFAGPAVYVVMLSLALWIVYQTGWSNISFTLASKILSPSEQVWQMLTATALVV
SYFAGPLLNFGDFSRYGKSMQEIRRGNRWGLPFNFLLFSLVTVVIVSGSQSLFGEMITDPIETVTRVGNGVAIALGLLTM
IIATIGINIVANFVSPAFDFSNCSPQRINFRTGGMIAAVGSVLLTPWNLFQSPIIIHYTLDVLGAFMGPLFGILIADFYL
IKRGQLYVDDLFNASSSGRYWYRGGFNPKAIMALLPAVAIGLMIIFIPNLQQISSFSWFIGAFLGGSIYRFIARNDPLPN
NAALFIRPEIGKD

Specific function: Transport of allantoin [H]

COG id: COG1953

COG function: function code FH; Cytosine/uracil/thiamine/allantoin permeases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purine-cytosine permease family [H]

Homologues:

Organism=Escherichia coli, GI87081749, Length=459, Percent_Identity=28.7581699346405, Blast_Score=207, Evalue=9e-55,
Organism=Saccharomyces cerevisiae, GI6322219, Length=438, Percent_Identity=23.0593607305936, Blast_Score=137, Evalue=3e-33,
Organism=Saccharomyces cerevisiae, GI6319495, Length=435, Percent_Identity=22.5287356321839, Blast_Score=135, Evalue=1e-32,
Organism=Saccharomyces cerevisiae, GI6319429, Length=438, Percent_Identity=21.0045662100457, Blast_Score=114, Evalue=2e-26,
Organism=Saccharomyces cerevisiae, GI6323266, Length=439, Percent_Identity=23.0068337129841, Blast_Score=104, Evalue=3e-23,
Organism=Saccharomyces cerevisiae, GI6324766, Length=439, Percent_Identity=22.0956719817768, Blast_Score=104, Evalue=3e-23,
Organism=Saccharomyces cerevisiae, GI6324645, Length=446, Percent_Identity=21.52466367713, Blast_Score=99, Evalue=2e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001248
- InterPro:   IPR012681 [H]

Pfam domain/function: PF02133 Transp_cyt_pur [H]

EC number: NA

Molecular weight: Translated: 54482; Mature: 54351

Theoretical pI: Translated: 9.48; Mature: 9.48

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPNNEKISASSINPHYSPKLTNEDLAPTRKQNWGWYNIFSFWMTDVHSMGGYVVAASFFA
CCCCCCEECCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHH
LGLSSWQVLLSLLVGICIVQICANLVAKPSQIAGVPYAVICRQSFGVFGANIPAVIRGLI
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCHHHHHHHHCCCCCCCHHHHHHHHH
AVAWYGIQTYLAGNAFMLVMLKFFPSLIPLAQSQWLGLSTLGWICFSIMWVLQAMVFWHG
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
MNAIKRFIDFAGPAVYVVMLSLALWIVYQTGWSNISFTLASKILSPSEQVWQMLTATALV
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHH
VSYFAGPLLNFGDFSRYGKSMQEIRRGNRWGLPFNFLLFSLVTVVIVSGSQSLFGEMITD
HHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHH
PIETVTRVGNGVAIALGLLTMIIATIGINIVANFVSPAFDFSNCSPQRINFRTGGMIAAV
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEECCCCCHHHHH
GSVLLTPWNLFQSPIIIHYTLDVLGAFMGPLFGILIADFYLIKRGQLYVDDLFNASSSGR
HHHHHCCHHHHCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCC
YWYRGGFNPKAIMALLPAVAIGLMIIFIPNLQQISSFSWFIGAFLGGSIYRFIARNDPLP
EEEECCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
NNAALFIRPEIGKD
CCCEEEEECCCCCC
>Mature Secondary Structure 
PNNEKISASSINPHYSPKLTNEDLAPTRKQNWGWYNIFSFWMTDVHSMGGYVVAASFFA
CCCCCEECCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHH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CCCEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9353933; 9384377; 11344136 [H]