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The map label for this gene is proS

Identifier: 218928237

GI number: 218928237

Start: 1210676

End: 1212394

Strand: Reverse

Name: proS

Synonym: YPO1068

Alternate gene names: 218928237

Gene position: 1212394-1210676 (Counterclockwise)

Preceding gene: 218928238

Following gene: 218928233

Centisome position: 26.05

GC content: 49.91

Gene sequence:

>1719_bases
ATGCGTACTAGCCAATATCTGCTCTCCACTCAGAAAGAGACACCTGCCGATGCTGAAGTTATCAGCCATCAACTCATGCT
CCGTGCCGGGATGATCCGCAAACTGGCCTCAGGTCTTTACACCTGGCTACCGACCGGGGTTCGGGTGTTGAAAAAAGTTG
AAAACATCGTGCGCGAAGAAATGAATAACGCAGGTGCTATCGAAGTATCAATGCCAGTGGTACAACCCGCGGACTTATGG
CAAGAGAGTGGCCGCTGGGAGCAGTACGGCCCTGAGTTGTTGCGTTTTGTTGACCGCGGCGAGCGCCCTTTTGTTCTTGG
CCCAACCCATGAAGAAGTGATTACTGATCTGATCCGCGGCGAGATTAACTCTTACAAACAACTGCCGCTGAATTTCTTCC
AAATTCAGACCAAGTTTCGTGATGAAGTGCGCCCGCGCTTTGGTGTCATGCGCGCCCGTGAGTTTTTGATGAAAGATGCG
TATTCCTTCCATACTACGCAAGAGTCTTTACAGGAAACTTACGACGCGATGTATACCGCGTACAGTAAGATTTTCAGCCG
GATGGATTTGAACTTCCGTGCGGTACTGGCGGATACCGGCTCTATCGGCGGCAGTGCATCCCACGAATTCCAAGTGCTGG
CAGAAAGTGGCGAAGATGATATCGTTTTCTCCACTGGCTCTGATTACGCAGCCAATATTGAATTCGCTGAAGCATTAGCG
CCAACTGAACCACGCGCACCGGCTACAGAAGAGCTGCGAATCGTTGACACCCCGAACGCAAAAACCATCGCCGAGTTGGT
AGAGCAATTCAAACTACCCATTGAGAAAACCGTGAAAACCTTATTGGTTCATGCTCACGAAGAGAGTGGTCATAAGTTGG
TTGCGCTCCTGGTGCGCGGCGATCATGATTTAAATGAGATCAAAGCAGAGAAGCTGCCACAAGTTGCCAAGCCACTGACC
TTTGCTAGCGAAGAGGAAATTCGTGCAGCTATCGGCGCTGGCCCAGGTTCATTAGGCCCGGTTAATTTATCATTGCCTGT
TATTGCTGACCACAGTGTGGCGGTGATGAGCGATTTCGGTGCGGGTGCGAATATTGATGGGAAGCATTACTTTGGTATTA
ACTGGGAGCGTGATTTGGCGCTGCCGCTCGTTGCTGATTTGCGTAACGTAGTAGAAGGCGATATCAGCCCTGATGGCAAA
GGCACTCTACAGATCAAACGCGGTATTGAAGTGGGCCATATTTTCCAGTTAGGGACCAAATATTCTGAAGTCATGAAAGC
CACCGTTCAGGGCGAAGATGGCCGTAATCAGGTGATGACCATGGGTTGCTACGGCATAGGGGTATCCCGTGTGGTGGCGG
CGGCTATTGAGCAGAACCATGATGACCGCGGTATCATCTGGCCAGATGCTATTGCGCCATTCCAGGTTGCTATTCTACCT
ATGAATATGCACAAATCTTTCCGAGTGAAAGAACTGGCAGAAGAGCTCTACACCACCCTGCGTTCTCACGGTATTGACGT
TATTCTCGACGACCGTAAAGAGCGCCCAGGTGTGATGTTTGCCGATATGGAATTGATTGGCGTGCCACACAATATCGTCA
TTGGTGACCGTAATCTCGATAGCGAAGAAGTGGAATACAAAAACCGACGCGTCGGTGAGAAACAAATGATTAAGACCAGC
GAAATTGTTGAGTTCTTGCTGAGCCAAATAAAACGCTGA

Upstream 100 bases:

>100_bases
TCACTCTGCTCTCTTTTGACAACCCGTGCTCACTGGTAAACTAGGCCACTTTTTACTTTTTGGCTGCCCCTAATTGCAAT
GCTAATGGAACAAAAACATC

Downstream 100 bases:

>100_bases
ATGGCTATATACCCGTCATAAATAAAGATACCCGCCATAAATAAAGAGTCCCCATAAGCGGGGTATTTTACTGATGAAGC
CGCCGGAGAACTTGAACTAC

Product: prolyl-tRNA synthetase

Products: NA

Alternate protein names: Proline--tRNA ligase; ProRS

Number of amino acids: Translated: 572; Mature: 572

Protein sequence:

>572_residues
MRTSQYLLSTQKETPADAEVISHQLMLRAGMIRKLASGLYTWLPTGVRVLKKVENIVREEMNNAGAIEVSMPVVQPADLW
QESGRWEQYGPELLRFVDRGERPFVLGPTHEEVITDLIRGEINSYKQLPLNFFQIQTKFRDEVRPRFGVMRAREFLMKDA
YSFHTTQESLQETYDAMYTAYSKIFSRMDLNFRAVLADTGSIGGSASHEFQVLAESGEDDIVFSTGSDYAANIEFAEALA
PTEPRAPATEELRIVDTPNAKTIAELVEQFKLPIEKTVKTLLVHAHEESGHKLVALLVRGDHDLNEIKAEKLPQVAKPLT
FASEEEIRAAIGAGPGSLGPVNLSLPVIADHSVAVMSDFGAGANIDGKHYFGINWERDLALPLVADLRNVVEGDISPDGK
GTLQIKRGIEVGHIFQLGTKYSEVMKATVQGEDGRNQVMTMGCYGIGVSRVVAAAIEQNHDDRGIIWPDAIAPFQVAILP
MNMHKSFRVKELAEELYTTLRSHGIDVILDDRKERPGVMFADMELIGVPHNIVIGDRNLDSEEVEYKNRRVGEKQMIKTS
EIVEFLLSQIKR

Sequences:

>Translated_572_residues
MRTSQYLLSTQKETPADAEVISHQLMLRAGMIRKLASGLYTWLPTGVRVLKKVENIVREEMNNAGAIEVSMPVVQPADLW
QESGRWEQYGPELLRFVDRGERPFVLGPTHEEVITDLIRGEINSYKQLPLNFFQIQTKFRDEVRPRFGVMRAREFLMKDA
YSFHTTQESLQETYDAMYTAYSKIFSRMDLNFRAVLADTGSIGGSASHEFQVLAESGEDDIVFSTGSDYAANIEFAEALA
PTEPRAPATEELRIVDTPNAKTIAELVEQFKLPIEKTVKTLLVHAHEESGHKLVALLVRGDHDLNEIKAEKLPQVAKPLT
FASEEEIRAAIGAGPGSLGPVNLSLPVIADHSVAVMSDFGAGANIDGKHYFGINWERDLALPLVADLRNVVEGDISPDGK
GTLQIKRGIEVGHIFQLGTKYSEVMKATVQGEDGRNQVMTMGCYGIGVSRVVAAAIEQNHDDRGIIWPDAIAPFQVAILP
MNMHKSFRVKELAEELYTTLRSHGIDVILDDRKERPGVMFADMELIGVPHNIVIGDRNLDSEEVEYKNRRVGEKQMIKTS
EIVEFLLSQIKR
>Mature_572_residues
MRTSQYLLSTQKETPADAEVISHQLMLRAGMIRKLASGLYTWLPTGVRVLKKVENIVREEMNNAGAIEVSMPVVQPADLW
QESGRWEQYGPELLRFVDRGERPFVLGPTHEEVITDLIRGEINSYKQLPLNFFQIQTKFRDEVRPRFGVMRAREFLMKDA
YSFHTTQESLQETYDAMYTAYSKIFSRMDLNFRAVLADTGSIGGSASHEFQVLAESGEDDIVFSTGSDYAANIEFAEALA
PTEPRAPATEELRIVDTPNAKTIAELVEQFKLPIEKTVKTLLVHAHEESGHKLVALLVRGDHDLNEIKAEKLPQVAKPLT
FASEEEIRAAIGAGPGSLGPVNLSLPVIADHSVAVMSDFGAGANIDGKHYFGINWERDLALPLVADLRNVVEGDISPDGK
GTLQIKRGIEVGHIFQLGTKYSEVMKATVQGEDGRNQVMTMGCYGIGVSRVVAAAIEQNHDDRGIIWPDAIAPFQVAILP
MNMHKSFRVKELAEELYTTLRSHGIDVILDDRKERPGVMFADMELIGVPHNIVIGDRNLDSEEVEYKNRRVGEKQMIKTS
EIVEFLLSQIKR

Specific function: Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction:proline is first activated by ATP to form Pro- AMP and then transferred to the acceptor end of tRNA(Pro). As ProRS can inadvertently accommodate and process non-cognate amino acids su

COG id: COG0442

COG function: function code J; Prolyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 1 subfamily

Homologues:

Organism=Homo sapiens, GI34303926, Length=219, Percent_Identity=44.7488584474886, Blast_Score=209, Evalue=6e-54,
Organism=Escherichia coli, GI1786392, Length=571, Percent_Identity=84.4133099824869, Blast_Score=1016, Evalue=0.0,
Organism=Caenorhabditis elegans, GI115532348, Length=212, Percent_Identity=41.5094339622642, Blast_Score=174, Evalue=1e-43,
Organism=Caenorhabditis elegans, GI193203271, Length=89, Percent_Identity=38.2022471910112, Blast_Score=81, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6320931, Length=550, Percent_Identity=30.7272727272727, Blast_Score=245, Evalue=2e-65,
Organism=Drosophila melanogaster, GI24656200, Length=220, Percent_Identity=37.7272727272727, Blast_Score=177, Evalue=1e-44,

Paralogues:

None

Copy number: 800 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): SYP_YERPA (Q1CAK9)

Other databases:

- EMBL:   CP000308
- RefSeq:   YP_650458.1
- ProteinModelPortal:   Q1CAK9
- STRING:   Q1CAK9
- GeneID:   4122306
- GenomeReviews:   CP000308_GR
- KEGG:   ypa:YPA_0545
- eggNOG:   COG0442
- HOGENOM:   HBG403504
- OMA:   DFVLGPT
- ProtClustDB:   PRK09194
- BioCyc:   YPES360102:YPA_0545-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01569
- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR002316
- InterPro:   IPR004500
- InterPro:   IPR007214
- Gene3D:   G3DSA:3.40.50.800
- Gene3D:   G3DSA:3.90.960.10
- PRINTS:   PR01046
- TIGRFAMs:   TIGR00409

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b; PF04073 YbaK; SSF52954 Anticodon_bd; SSF55826 YbaK/aa-tRNA-synth-assoc-reg

EC number: =6.1.1.15

Molecular weight: Translated: 63857; Mature: 63857

Theoretical pI: Translated: 5.11; Mature: 5.11

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRTSQYLLSTQKETPADAEVISHQLMLRAGMIRKLASGLYTWLPTGVRVLKKVENIVREE
CCCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MNNAGAIEVSMPVVQPADLWQESGRWEQYGPELLRFVDRGERPFVLGPTHEEVITDLIRG
HCCCCEEEEECCCCCCHHHHHHCCCCHHHCHHHHHHHHCCCCCEEECCCHHHHHHHHHHH
EINSYKQLPLNFFQIQTKFRDEVRPRFGVMRAREFLMKDAYSFHTTQESLQETYDAMYTA
HHHHHHHCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YSKIFSRMDLNFRAVLADTGSIGGSASHEFQVLAESGEDDIVFSTGSDYAANIEFAEALA
HHHHHHHCCCCEEEEEEECCCCCCCCCCCEEEEECCCCCCEEEECCCCCEECCHHHHHCC
PTEPRAPATEELRIVDTPNAKTIAELVEQFKLPIEKTVKTLLVHAHEESGHKLVALLVRG
CCCCCCCCCCCEEEEECCCHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCEEEEEEEEC
DHDLNEIKAEKLPQVAKPLTFASEEEIRAAIGAGPGSLGPVNLSLPVIADHSVAVMSDFG
CCCHHHHHHHHCHHHHCCCCCCCHHHHHHHHCCCCCCCCCEEEEEEEEECCCEEEEECCC
AGANIDGKHYFGINWERDLALPLVADLRNVVEGDISPDGKGTLQIKRGIEVGHIFQLGTK
CCCCCCCCEEECCCCCCCCCCHHHHHHHHHHCCCCCCCCCCEEEEECCCCCHHHHHHHHH
YSEVMKATVQGEDGRNQVMTMGCYGIGVSRVVAAAIEQNHDDRGIIWPDAIAPFQVAILP
HHHHHHHHHCCCCCCCCEEEEEEHHCCHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEE
MNMHKSFRVKELAEELYTTLRSHGIDVILDDRKERPGVMFADMELIGVPHNIVIGDRNLD
ECCCCCHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCEEEECEEEECCCCEEEEECCCCC
SEEVEYKNRRVGEKQMIKTSEIVEFLLSQIKR
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MRTSQYLLSTQKETPADAEVISHQLMLRAGMIRKLASGLYTWLPTGVRVLKKVENIVREE
CCCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MNNAGAIEVSMPVVQPADLWQESGRWEQYGPELLRFVDRGERPFVLGPTHEEVITDLIRG
HCCCCEEEEECCCCCCHHHHHHCCCCHHHCHHHHHHHHCCCCCEEECCCHHHHHHHHHHH
EINSYKQLPLNFFQIQTKFRDEVRPRFGVMRAREFLMKDAYSFHTTQESLQETYDAMYTA
HHHHHHHCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YSKIFSRMDLNFRAVLADTGSIGGSASHEFQVLAESGEDDIVFSTGSDYAANIEFAEALA
HHHHHHHCCCCEEEEEEECCCCCCCCCCCEEEEECCCCCCEEEECCCCCEECCHHHHHCC
PTEPRAPATEELRIVDTPNAKTIAELVEQFKLPIEKTVKTLLVHAHEESGHKLVALLVRG
CCCCCCCCCCCEEEEECCCHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCEEEEEEEEC
DHDLNEIKAEKLPQVAKPLTFASEEEIRAAIGAGPGSLGPVNLSLPVIADHSVAVMSDFG
CCCHHHHHHHHCHHHHCCCCCCCHHHHHHHHCCCCCCCCCEEEEEEEEECCCEEEEECCC
AGANIDGKHYFGINWERDLALPLVADLRNVVEGDISPDGKGTLQIKRGIEVGHIFQLGTK
CCCCCCCCEEECCCCCCCCCCHHHHHHHHHHCCCCCCCCCCEEEEECCCCCHHHHHHHHH
YSEVMKATVQGEDGRNQVMTMGCYGIGVSRVVAAAIEQNHDDRGIIWPDAIAPFQVAILP
HHHHHHHHHCCCCCCCCEEEEEEHHCCHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEE
MNMHKSFRVKELAEELYTTLRSHGIDVILDDRKERPGVMFADMELIGVPHNIVIGDRNLD
ECCCCCHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCEEEECEEEECCCCEEEEECCCCC
SEEVEYKNRRVGEKQMIKTSEIVEFLLSQIKR
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA