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The map label for this gene is ygfZ [C]

Identifier: 218928072

GI number: 218928072

Start: 983357

End: 984349

Strand: Direct

Name: ygfZ [C]

Synonym: YPO0898

Alternate gene names: 218928072

Gene position: 983357-984349 (Clockwise)

Preceding gene: 218928066

Following gene: 218928085

Centisome position: 21.13

GC content: 51.86

Gene sequence:

>993_bases
ATGGCCTATCATACTCCGTTTGCTGCACAGCCACCGGTCGCCTCTTCCGGGCTACCCTTAACACTGATCTCACTCGATGA
CTGGGCATTGGTTACCTTAACGGGTGCTGATCGGGTCAAATATCTACAAGGACAAGTCACGGCGGATATCGATGCGCTAT
CTGCTGATCAACATGTACTCTGTGCGCACTGTGATGCCAAAGGGAAAATGTGGAGTAACTTACGGCTGTTTTATCGTGGT
GAGGGGTTGGCCTTTATAGAGCGGCGCAGCCTGCTAGATAACCAGTTGAGTGAGTTGAAGAAATACGCTGTATTCTCCAA
GGTGGTCATTGAACCACAGCCAGATGCGGTACTCATTGGGGTTGCAGGTAGCCAGGCCAAAACCGCGCTGGCGGAAATCT
TTACTGAATTGCCCAGTGCAGAACACCCGGTAACGCAGATGGGTAATAGCACGCTGTTACACTTCTCACTGCCTGCTGAA
CGTTTTCTGTTAGTAACGGATACAGAGCAGGCGCAGCAGTTGGTTGAGAAACTGGCTGGTCGGGCGCAATTTAACGACAG
CAAACAGTGGCTGGCACTGGACATCGAGGCCGGTTTCCCCATCATTGATGCTGCTAACAGTGCACAGTTTATTCCTCAGG
CAACAAATATTCAGGCGTTAAATGGTATCAGCTTCACGAAAGGCTGTTATACCGGGCAGGAAATGGTCGCCAGAGCAAAG
TACCGGGGTGCCAATAAGCGGGCGCTGTATTGGTTGGCAGGTAATGCCAGCCGAGTACCCGCAGCAGGTGAAGATTTAGA
GTGGCAGCTAGGCGAGAATTGGCGTCGTACGGGTACCGTGCTGTCAGCCATTCAACTCAATGATGGCACCGTGTGGGTTC
AGGCTGTGTTGAATAATGATCTGGCCGCAGATAGCGTGTTGCGGGTACGTGATGATGCGCTGGGTACGCTAGCCATTCAG
CCTTTGCCCTACTCACTCGCCGAAGATAAATAA

Upstream 100 bases:

>100_bases
AGGCGAACAATGGTGAATCTATGGAATTACTGCTTGCAAAGCTACCCGGCTATTTTACCATTGGGCAATTAGCTCACCGT
TTCTCATTCCAGGATGACAT

Downstream 100 bases:

>100_bases
TAAAGAGTTCTACTCTACAGAGACCGGTTAACCGGTCTCTGTATTTTGTTTTATATCAAATAGATGGGTGGGTTATTCAT
CTAACTCGCCATCAGGCTGT

Product: putative global regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 330; Mature: 329

Protein sequence:

>330_residues
MAYHTPFAAQPPVASSGLPLTLISLDDWALVTLTGADRVKYLQGQVTADIDALSADQHVLCAHCDAKGKMWSNLRLFYRG
EGLAFIERRSLLDNQLSELKKYAVFSKVVIEPQPDAVLIGVAGSQAKTALAEIFTELPSAEHPVTQMGNSTLLHFSLPAE
RFLLVTDTEQAQQLVEKLAGRAQFNDSKQWLALDIEAGFPIIDAANSAQFIPQATNIQALNGISFTKGCYTGQEMVARAK
YRGANKRALYWLAGNASRVPAAGEDLEWQLGENWRRTGTVLSAIQLNDGTVWVQAVLNNDLAADSVLRVRDDALGTLAIQ
PLPYSLAEDK

Sequences:

>Translated_330_residues
MAYHTPFAAQPPVASSGLPLTLISLDDWALVTLTGADRVKYLQGQVTADIDALSADQHVLCAHCDAKGKMWSNLRLFYRG
EGLAFIERRSLLDNQLSELKKYAVFSKVVIEPQPDAVLIGVAGSQAKTALAEIFTELPSAEHPVTQMGNSTLLHFSLPAE
RFLLVTDTEQAQQLVEKLAGRAQFNDSKQWLALDIEAGFPIIDAANSAQFIPQATNIQALNGISFTKGCYTGQEMVARAK
YRGANKRALYWLAGNASRVPAAGEDLEWQLGENWRRTGTVLSAIQLNDGTVWVQAVLNNDLAADSVLRVRDDALGTLAIQ
PLPYSLAEDK
>Mature_329_residues
AYHTPFAAQPPVASSGLPLTLISLDDWALVTLTGADRVKYLQGQVTADIDALSADQHVLCAHCDAKGKMWSNLRLFYRGE
GLAFIERRSLLDNQLSELKKYAVFSKVVIEPQPDAVLIGVAGSQAKTALAEIFTELPSAEHPVTQMGNSTLLHFSLPAER
FLLVTDTEQAQQLVEKLAGRAQFNDSKQWLALDIEAGFPIIDAANSAQFIPQATNIQALNGISFTKGCYTGQEMVARAKY
RGANKRALYWLAGNASRVPAAGEDLEWQLGENWRRTGTVLSAIQLNDGTVWVQAVLNNDLAADSVLRVRDDALGTLAIQP
LPYSLAEDK

Specific function: Folate-binding protein involved in regulating the level of ATP-dnaA and in the modification of some tRNAs. It is probably a key factor in regulatory networks that act via tRNA modification, such as initiation of chromosomal replication [H]

COG id: COG0354

COG function: function code R; Predicted aminomethyltransferase related to GcvT

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA-modifying ygfZ family [H]

Homologues:

Organism=Escherichia coli, GI1789265, Length=328, Percent_Identity=67.9878048780488, Blast_Score=449, Evalue=1e-127,

Paralogues:

None

Copy number: 40 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017703
- InterPro:   IPR006222 [H]

Pfam domain/function: PF01571 GCV_T [H]

EC number: NA

Molecular weight: Translated: 35966; Mature: 35835

Theoretical pI: Translated: 5.02; Mature: 5.02

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAYHTPFAAQPPVASSGLPLTLISLDDWALVTLTGADRVKYLQGQVTADIDALSADQHVL
CCCCCCCCCCCCCCCCCCCEEEEEECCEEEEEEECCCCEEEECCCEEECCCCCCCCCEEE
CAHCDAKGKMWSNLRLFYRGEGLAFIERRSLLDNQLSELKKYAVFSKVVIEPQPDAVLIG
EEECCCCCCCCCCEEEEEECCCEEEHHHHHHHHHHHHHHHHHHHHHHEEECCCCCEEEEE
VAGSQAKTALAEIFTELPSAEHPVTQMGNSTLLHFSLPAERFLLVTDTEQAQQLVEKLAG
ECCCHHHHHHHHHHHHCCCCCCCHHHCCCCEEEEEECCCCEEEEEECCHHHHHHHHHHHC
RAQFNDSKQWLALDIEAGFPIIDAANSAQFIPQATNIQALNGISFTKGCYTGQEMVARAK
CCCCCCCCCEEEEEECCCCCEEECCCCCCCCCCCCCEEEECCCCCCCCCCCCHHHHHHHH
YRGANKRALYWLAGNASRVPAAGEDLEWQLGENWRRTGTVLSAIQLNDGTVWVQAVLNND
HCCCCCEEEEEEECCCCCCCCCCCCCEEECCCCCHHCCEEEEEEEECCCEEEEEEECCCC
LAADSVLRVRDDALGTLAIQPLPYSLAEDK
CCCCCEEEECCCCCEEEEEECCCCCCCCCC
>Mature Secondary Structure 
AYHTPFAAQPPVASSGLPLTLISLDDWALVTLTGADRVKYLQGQVTADIDALSADQHVL
CCCCCCCCCCCCCCCCCCEEEEEECCEEEEEEECCCCEEEECCCEEECCCCCCCCCEEE
CAHCDAKGKMWSNLRLFYRGEGLAFIERRSLLDNQLSELKKYAVFSKVVIEPQPDAVLIG
EEECCCCCCCCCCEEEEEECCCEEEHHHHHHHHHHHHHHHHHHHHHHEEECCCCCEEEEE
VAGSQAKTALAEIFTELPSAEHPVTQMGNSTLLHFSLPAERFLLVTDTEQAQQLVEKLAG
ECCCHHHHHHHHHHHHCCCCCCCHHHCCCCEEEEEECCCCEEEEEECCHHHHHHHHHHHC
RAQFNDSKQWLALDIEAGFPIIDAANSAQFIPQATNIQALNGISFTKGCYTGQEMVARAK
CCCCCCCCCEEEEEECCCCCEEECCCCCCCCCCCCCEEEECCCCCCCCCCCCHHHHHHHH
YRGANKRALYWLAGNASRVPAAGEDLEWQLGENWRRTGTVLSAIQLNDGTVWVQAVLNND
HCCCCCEEEEEEECCCCCCCCCCCCCEEECCCCCHHCCEEEEEEEECCCEEEEEEECCCC
LAADSVLRVRDDALGTLAIQPLPYSLAEDK
CCCCCEEEECCCCCEEEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA