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The map label for this gene is lysS [H]

Identifier: 218928061

GI number: 218928061

Start: 971744

End: 973261

Strand: Reverse

Name: lysS [H]

Synonym: YPO0888

Alternate gene names: 218928061

Gene position: 973261-971744 (Counterclockwise)

Preceding gene: 218928063

Following gene: 218928060

Centisome position: 20.91

GC content: 47.96

Gene sequence:

>1518_bases
ATGTCAGAGCAAAAACCACAAGTCGCTGAGCAAGCGCAAGAACTCAATAGTGAGTTACAAGCCCGTCGAGAAAAACTGGC
TGTCTTACGGGGAAAGGGAATTGCATTCCCTAATGATTTCCGCCGTGAGAACCTTTCCGACCAGTTGCATGCCGAGTTTG
ACAGTAAAGAGAATGAAGAGTTAGAAGCACTGAATATCGACGTAACCGTTGCTGGCCGTATGATGACCCGCCGTATCATG
GGTAAAGCATCATTTGTGACTCTGCAAGATGTTGGTGGCCGTATCCAGTTGTACGTTTCCCGTGATGACCTACCGGAAGG
CGTTTATAACGAAGAATTCAAAAAGTGGGACCTGGGCGATATCCTGGGTGCGCGCGGTAAACTGTTTAAAACCAAAACCG
GTGAGCTGTCGATCCACTGTAGCGAACTGCGTCTGTTGACCAAGGCTCTGCGTCCATTGCCAGATAAATTCCATGGCTTA
GCTGACCAGGAAACCCGTTACCGCCAGCGTTATTTGGATCTGATTGCTAACGACGAGTCTCGTCATACTTTCAAAGTCCG
TTCGCAAGTGATGTCCGGTATCCGTAGCTTCATGGTTGAAAAAGGCTTCATGGAAGTCGAAACGCCAATGATGCAAGTGA
TCCCTGGCGGCGCTTCCGCACGTCCGTTTGTCACGCATCACAATGCGTTGGATATCGATATGTATTTGCGTATCGCGCCA
GAGCTGTATCTGAAACGTCTGGTCGTGGGGGGCTTCGAACGTGTGTTCGAAATCAACCGTAACTTCCGCAACGAAGGGGT
TTCGCCACGTCATAACCCAGAGTTCACCATGATGGAACTCTATATGGCTTATGCGGACTATAAAGACCTGATCGTCTTGA
CGGAAGAGCTATTCCGCACTCTGACCGAAACCATATTGGGGAGCAGTGTGGTGCAATATGGCGAACAGACTTTTGATTTC
GGTAAGCCTTTTGCCAAATTGACCATGAAAGAAGCTATTTGTAAATATCGTCCTGAAACCAATGTCGCGGATTTAGACGA
TATGGATAAAGCCGTCGCAATTGCCGAATCATTGGGTATCAAGGTTGAGAAGAGCTGGGGCCTGGGTCGTATTCAATGTG
AAATCTTTGAAGAGACCGCAGAAAGTCATCTTATCCAGCCAACGTTCATCACAGAATATCCAGCCGAGGTTTCTCCGCTA
GCACGCCGTAATGACGATAATCCATTTATCACTGACCGCTTTGAATTCTTCATCGGTGGCCGTGAAATTGGTAATGGCTT
CTCTGAGTTGAACGATGCCGAAGACCAGGCGCAGCGTTTCGCCGATCAGGTAAGTGCTAAAGAAGCTGGCGATGATGAAG
CGATGTTCTACGATGAAGACTATATCACTGCATTGGAACATGGCTTACCACCAACCGCAGGGCTAGGTATTGGTATTGAC
CGTATGGTTATGTTGTTTACCAATAGCCATACCATCCGTGATGTCATTTTATTCCCAGCGATGCGCCCGGTAAAATAA

Upstream 100 bases:

>100_bases
GCGTACCGGTGTGGAAACACGCAACACGCAAGCTGTACTGGATGGCGATCTGGACAAATTTATTGAAGCAAGCTTGAAAG
CCGGGCTATGAGGAACTGAG

Downstream 100 bases:

>100_bases
GCGACACGGTTTAACGATAAATTCCTCAGGGCGCTACGGCGCCCTTTTTACTGAGTCAGCTAAACTTGTTTCTTGAAGTA
AAACGGTGTTAGTGATGTAT

Product: lysyl-tRNA synthetase

Products: NA

Alternate protein names: Lysine--tRNA ligase; LysRS [H]

Number of amino acids: Translated: 505; Mature: 504

Protein sequence:

>505_residues
MSEQKPQVAEQAQELNSELQARREKLAVLRGKGIAFPNDFRRENLSDQLHAEFDSKENEELEALNIDVTVAGRMMTRRIM
GKASFVTLQDVGGRIQLYVSRDDLPEGVYNEEFKKWDLGDILGARGKLFKTKTGELSIHCSELRLLTKALRPLPDKFHGL
ADQETRYRQRYLDLIANDESRHTFKVRSQVMSGIRSFMVEKGFMEVETPMMQVIPGGASARPFVTHHNALDIDMYLRIAP
ELYLKRLVVGGFERVFEINRNFRNEGVSPRHNPEFTMMELYMAYADYKDLIVLTEELFRTLTETILGSSVVQYGEQTFDF
GKPFAKLTMKEAICKYRPETNVADLDDMDKAVAIAESLGIKVEKSWGLGRIQCEIFEETAESHLIQPTFITEYPAEVSPL
ARRNDDNPFITDRFEFFIGGREIGNGFSELNDAEDQAQRFADQVSAKEAGDDEAMFYDEDYITALEHGLPPTAGLGIGID
RMVMLFTNSHTIRDVILFPAMRPVK

Sequences:

>Translated_505_residues
MSEQKPQVAEQAQELNSELQARREKLAVLRGKGIAFPNDFRRENLSDQLHAEFDSKENEELEALNIDVTVAGRMMTRRIM
GKASFVTLQDVGGRIQLYVSRDDLPEGVYNEEFKKWDLGDILGARGKLFKTKTGELSIHCSELRLLTKALRPLPDKFHGL
ADQETRYRQRYLDLIANDESRHTFKVRSQVMSGIRSFMVEKGFMEVETPMMQVIPGGASARPFVTHHNALDIDMYLRIAP
ELYLKRLVVGGFERVFEINRNFRNEGVSPRHNPEFTMMELYMAYADYKDLIVLTEELFRTLTETILGSSVVQYGEQTFDF
GKPFAKLTMKEAICKYRPETNVADLDDMDKAVAIAESLGIKVEKSWGLGRIQCEIFEETAESHLIQPTFITEYPAEVSPL
ARRNDDNPFITDRFEFFIGGREIGNGFSELNDAEDQAQRFADQVSAKEAGDDEAMFYDEDYITALEHGLPPTAGLGIGID
RMVMLFTNSHTIRDVILFPAMRPVK
>Mature_504_residues
SEQKPQVAEQAQELNSELQARREKLAVLRGKGIAFPNDFRRENLSDQLHAEFDSKENEELEALNIDVTVAGRMMTRRIMG
KASFVTLQDVGGRIQLYVSRDDLPEGVYNEEFKKWDLGDILGARGKLFKTKTGELSIHCSELRLLTKALRPLPDKFHGLA
DQETRYRQRYLDLIANDESRHTFKVRSQVMSGIRSFMVEKGFMEVETPMMQVIPGGASARPFVTHHNALDIDMYLRIAPE
LYLKRLVVGGFERVFEINRNFRNEGVSPRHNPEFTMMELYMAYADYKDLIVLTEELFRTLTETILGSSVVQYGEQTFDFG
KPFAKLTMKEAICKYRPETNVADLDDMDKAVAIAESLGIKVEKSWGLGRIQCEIFEETAESHLIQPTFITEYPAEVSPLA
RRNDDNPFITDRFEFFIGGREIGNGFSELNDAEDQAQRFADQVSAKEAGDDEAMFYDEDYITALEHGLPPTAGLGIGIDR
MVMLFTNSHTIRDVILFPAMRPVK

Specific function: Unknown

COG id: COG1190

COG function: function code J; Lysyl-tRNA synthetase (class II)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI5031815, Length=453, Percent_Identity=46.5783664459161, Blast_Score=387, Evalue=1e-107,
Organism=Homo sapiens, GI194272210, Length=453, Percent_Identity=46.5783664459161, Blast_Score=387, Evalue=1e-107,
Organism=Homo sapiens, GI45439306, Length=471, Percent_Identity=24.8407643312102, Blast_Score=106, Evalue=7e-23,
Organism=Homo sapiens, GI40789249, Length=312, Percent_Identity=25, Blast_Score=85, Evalue=1e-16,
Organism=Escherichia coli, GI1789256, Length=505, Percent_Identity=85.9405940594059, Blast_Score=906, Evalue=0.0,
Organism=Escherichia coli, GI1790571, Length=505, Percent_Identity=81.7821782178218, Blast_Score=875, Evalue=0.0,
Organism=Escherichia coli, GI87082379, Length=326, Percent_Identity=30.9815950920245, Blast_Score=144, Evalue=1e-35,
Organism=Escherichia coli, GI1788173, Length=259, Percent_Identity=27.027027027027, Blast_Score=86, Evalue=5e-18,
Organism=Escherichia coli, GI1787161, Length=471, Percent_Identity=22.5053078556263, Blast_Score=73, Evalue=5e-14,
Organism=Caenorhabditis elegans, GI17535925, Length=454, Percent_Identity=41.6299559471366, Blast_Score=347, Evalue=8e-96,
Organism=Caenorhabditis elegans, GI17535927, Length=454, Percent_Identity=41.6299559471366, Blast_Score=347, Evalue=9e-96,
Organism=Caenorhabditis elegans, GI71994340, Length=454, Percent_Identity=41.4096916299559, Blast_Score=346, Evalue=2e-95,
Organism=Caenorhabditis elegans, GI17551876, Length=353, Percent_Identity=27.1954674220963, Blast_Score=99, Evalue=5e-21,
Organism=Caenorhabditis elegans, GI32566633, Length=268, Percent_Identity=29.1044776119403, Blast_Score=96, Evalue=5e-20,
Organism=Saccharomyces cerevisiae, GI6320242, Length=460, Percent_Identity=42.1739130434783, Blast_Score=358, Evalue=1e-100,
Organism=Saccharomyces cerevisiae, GI6324256, Length=482, Percent_Identity=29.6680497925311, Blast_Score=181, Evalue=3e-46,
Organism=Saccharomyces cerevisiae, GI6323011, Length=468, Percent_Identity=26.0683760683761, Blast_Score=103, Evalue=7e-23,
Organism=Saccharomyces cerevisiae, GI6321807, Length=513, Percent_Identity=23.5867446393762, Blast_Score=83, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24640849, Length=484, Percent_Identity=42.5619834710744, Blast_Score=382, Evalue=1e-106,
Organism=Drosophila melanogaster, GI24640851, Length=484, Percent_Identity=42.5619834710744, Blast_Score=382, Evalue=1e-106,
Organism=Drosophila melanogaster, GI17136276, Length=464, Percent_Identity=23.9224137931034, Blast_Score=102, Evalue=5e-22,
Organism=Drosophila melanogaster, GI24584738, Length=296, Percent_Identity=27.027027027027, Blast_Score=90, Evalue=3e-18,

Paralogues:

None

Copy number: 1200 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR002313
- InterPro:   IPR018149
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365 [H]

Pfam domain/function: PF00152 tRNA-synt_2; PF01336 tRNA_anti [H]

EC number: =6.1.1.6 [H]

Molecular weight: Translated: 57578; Mature: 57447

Theoretical pI: Translated: 4.83; Mature: 4.83

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEQKPQVAEQAQELNSELQARREKLAVLRGKGIAFPNDFRRENLSDQLHAEFDSKENEE
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCCCHHHHHHCCCCCCCC
LEALNIDVTVAGRMMTRRIMGKASFVTLQDVGGRIQLYVSRDDLPEGVYNEEFKKWDLGD
EEEEEEEEEEHHHHHHHHHHCCCCEEEEECCCCEEEEEEECCCCCCCCCCHHHCCCCHHH
ILGARGKLFKTKTGELSIHCSELRLLTKALRPLPDKFHGLADQETRYRQRYLDLIANDES
HHCCCCCEEEECCCCEEEEHHHHHHHHHHHHCCCHHHCCCCCHHHHHHHHHHHHHCCCCC
RHTFKVRSQVMSGIRSFMVEKGFMEVETPMMQVIPGGASARPFVTHHNALDIDMYLRIAP
CHHHHHHHHHHHHHHHHHHHCCCHHHCCCHHHHCCCCCCCCCEEECCCCEEHHHHHHHHH
ELYLKRLVVGGFERVFEINRNFRNEGVSPRHNPEFTMMELYMAYADYKDLIVLTEELFRT
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCHHHHHHHHHHHHHH
LTETILGSSVVQYGEQTFDFGKPFAKLTMKEAICKYRPETNVADLDDMDKAVAIAESLGI
HHHHHHHHHHHHHCHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCC
KVEKSWGLGRIQCEIFEETAESHLIQPTFITEYPAEVSPLARRNDDNPFITDRFEFFIGG
EEECCCCCCEEEHHHHHHHHHHHCCCCCEECCCCCCCCHHHHCCCCCCEEECCHHEEECC
REIGNGFSELNDAEDQAQRFADQVSAKEAGDDEAMFYDEDYITALEHGLPPTAGLGIGID
HHHCCCHHHCCCHHHHHHHHHHHHHHHCCCCCCCEEECHHHHHHHHHCCCCCCCCCCCHH
RMVMLFTNSHTIRDVILFPAMRPVK
HEEEEEECCCHHHHHHHHCCCCCCC
>Mature Secondary Structure 
SEQKPQVAEQAQELNSELQARREKLAVLRGKGIAFPNDFRRENLSDQLHAEFDSKENEE
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCCCHHHHHHCCCCCCCC
LEALNIDVTVAGRMMTRRIMGKASFVTLQDVGGRIQLYVSRDDLPEGVYNEEFKKWDLGD
EEEEEEEEEEHHHHHHHHHHCCCCEEEEECCCCEEEEEEECCCCCCCCCCHHHCCCCHHH
ILGARGKLFKTKTGELSIHCSELRLLTKALRPLPDKFHGLADQETRYRQRYLDLIANDES
HHCCCCCEEEECCCCEEEEHHHHHHHHHHHHCCCHHHCCCCCHHHHHHHHHHHHHCCCCC
RHTFKVRSQVMSGIRSFMVEKGFMEVETPMMQVIPGGASARPFVTHHNALDIDMYLRIAP
CHHHHHHHHHHHHHHHHHHHCCCHHHCCCHHHHCCCCCCCCCEEECCCCEEHHHHHHHHH
ELYLKRLVVGGFERVFEINRNFRNEGVSPRHNPEFTMMELYMAYADYKDLIVLTEELFRT
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCHHHHHHHHHHHHHH
LTETILGSSVVQYGEQTFDFGKPFAKLTMKEAICKYRPETNVADLDDMDKAVAIAESLGI
HHHHHHHHHHHHHCHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCC
KVEKSWGLGRIQCEIFEETAESHLIQPTFITEYPAEVSPLARRNDDNPFITDRFEFFIGG
EEECCCCCCEEEHHHHHHHHHHHCCCCCEECCCCCCCCHHHHCCCCCCEEECCHHEEECC
REIGNGFSELNDAEDQAQRFADQVSAKEAGDDEAMFYDEDYITALEHGLPPTAGLGIGID
HHHCCCHHHCCCHHHHHHHHHHHHHHHCCCCCCCEEECHHHHHHHHHCCCCCCCCCCCHH
RMVMLFTNSHTIRDVILFPAMRPVK
HEEEEEECCCHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA