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The map label for this gene is angR [H]

Identifier: 218927958

GI number: 218927958

Start: 848635

End: 850287

Strand: Reverse

Name: angR [H]

Synonym: YPO0777

Alternate gene names: 218927958

Gene position: 850287-848635 (Counterclockwise)

Preceding gene: 218927959

Following gene: 218927957

Centisome position: 18.27

GC content: 53.48

Gene sequence:

>1653_bases
GTGAGGGAAAGTGCCCATGTCGGTGTAGCATTACCACGGGGTTGTGATCAGATCATTGCGGTACTGGCGATTCAGTGGCT
CGGTGCGGCTTATGTACCTATCAGTGTGGAATGGCCCGCGTGTCGCCGATCACAGGTTATCACTTTGGCTGATATCCATT
TCCTTATTGGTGATCGCACGCTGGGCTGGCCGGAAGAGGTCGATGTGCTTTCGGTGGAGAGTGAACCGGTTTCTGACGAG
CGCCCCACGCCACGGGTAGTGAGTGCAGATAGCCTGGCTTATCTTATTTTCACGTCCGGCAGTACGGGTGTCCCTAAAGG
GGTGGCGGTTAGCCATGGCGCGGCGGTCAATACCATTGAGTCGGTCAATCGTCAGCATCAAATCAACCCACAAGATACGG
CCTTGGCATTATCAGCGCTGTATTTTGACCTCTCCGTTTGGGATGTTTTTGGTGTGTTGTCTGCCGGCGCGAGGTTAGTC
CTTATTCCGCAGCAGGCACAGCGAGAGGCGGCAATATGGTTATCACTGGTCCAACAACATCAAGTGACGGTGTGGAACAG
TGTGCCAGCGTTATTAGAGATGATGCTGTTATTTAATGAACAGATATTTAATGAACAGGATGAACAGCCGCCAGCGTTGC
CCTCGCTGCGAGTGGTGATGCTCTCAGGGGATTGGATAGTACCGGAGTTGCCTCAACGCCTGCGGCGGTTTGCGCCAAAT
GCTCACTGTGTCGCCATGGGGGGGGCCACGGAAGCGGCTATCTGGTCCAATTATTGGATAGCAGATACAGCGCTAACCGG
GTGGTGCTCGGTGCCTTACGGCGTGCCATTGCCTAATCAGCAATTCCGAATTGTGAATGAGCAAGAAGAGGATTGCCCTG
ACTGGGTGGCGGGTGAGTTGTGGATTGGAGGGCAAGGGGTTGCACAGGGCTATTATGGCGATAGCGCCGGCACTGAACAG
CAATTTATCCTACGCGATGGGCAACGGTGGTACCGCACAGGGGATACTGGCCGCTATCGCCCTGACGCCATCATCGAGTT
TCTGGGGCGCAAGGACCAGCAAGTTAAAATCTCGGGTTACCGCGTAGAACTAGAAGAAATTACGTTAGCCCTAAAAAGCT
ATCCTTCGATCGAGGATGTGGTCGCTTTTGTTATTCAACACAATGACCGCTCTGTTTTAGCGGCTGTTGCTGTTACGCCA
ACCCCCCTGGATAGGCAGGCGGTGACCGCATTTCTTCGCGAGCGGCTACCGGAGTATGCCATCCCCTCCCGGTTAGGGCA
TTGCTGCGCATGGCCACTGACGGATAACGGGAAACGTGACCAGCATGCCCTCCGTGCCTTCGTCACACCGTCTTTCGAAC
CGACCGAAACACCTCAGGTAACCCAGCCCATGACGGCTATGGAGCAATTACTGTGCGAGCAGCTCCAGTTACTGCTGAAT
GTGGTGTCGATTCAATCTCATGACAACTTTTTTGCGCTTGGCGGCGACTCCTTTATTGCCATTCGCCTGACATCCGTATT
GCGCCGAAACTATGGGGTTGAACTCCCACTGTGGAAAATTTTCAGCGTACAGACAATCGCACAAATTGCTTTAGTTATGG
AGCCTGCACACCAGAGTGCAGGGCGTATTCAATTTGTGGAAGACTCAATTTGA

Upstream 100 bases:

>100_bases
GCCCAGCAGACCGCGTTAATTTGGGGGGGGCAGCCGCCTCAGCTATCAACAACTTGAACACGCTGTGCAACGGCTTGCAC
AGTATTTACACGAGCAAGGG

Downstream 100 bases:

>100_bases
GGAGGAATCATTATGAATATTAATCTAGATTCATCTTCAGCAACACAGGCCACCGATCTACTCGTGAACCTTGAACAACA
GGAGGTTCGTTTCTGGTTAG

Product: putative siderophore biosysnthesis protein

Products: pyrophosphate; AMP; enterobactin; pyrophosphate; L-Seryl-AMP [C]

Alternate protein names: NA

Number of amino acids: Translated: 550; Mature: 550

Protein sequence:

>550_residues
MRESAHVGVALPRGCDQIIAVLAIQWLGAAYVPISVEWPACRRSQVITLADIHFLIGDRTLGWPEEVDVLSVESEPVSDE
RPTPRVVSADSLAYLIFTSGSTGVPKGVAVSHGAAVNTIESVNRQHQINPQDTALALSALYFDLSVWDVFGVLSAGARLV
LIPQQAQREAAIWLSLVQQHQVTVWNSVPALLEMMLLFNEQIFNEQDEQPPALPSLRVVMLSGDWIVPELPQRLRRFAPN
AHCVAMGGATEAAIWSNYWIADTALTGWCSVPYGVPLPNQQFRIVNEQEEDCPDWVAGELWIGGQGVAQGYYGDSAGTEQ
QFILRDGQRWYRTGDTGRYRPDAIIEFLGRKDQQVKISGYRVELEEITLALKSYPSIEDVVAFVIQHNDRSVLAAVAVTP
TPLDRQAVTAFLRERLPEYAIPSRLGHCCAWPLTDNGKRDQHALRAFVTPSFEPTETPQVTQPMTAMEQLLCEQLQLLLN
VVSIQSHDNFFALGGDSFIAIRLTSVLRRNYGVELPLWKIFSVQTIAQIALVMEPAHQSAGRIQFVEDSI

Sequences:

>Translated_550_residues
MRESAHVGVALPRGCDQIIAVLAIQWLGAAYVPISVEWPACRRSQVITLADIHFLIGDRTLGWPEEVDVLSVESEPVSDE
RPTPRVVSADSLAYLIFTSGSTGVPKGVAVSHGAAVNTIESVNRQHQINPQDTALALSALYFDLSVWDVFGVLSAGARLV
LIPQQAQREAAIWLSLVQQHQVTVWNSVPALLEMMLLFNEQIFNEQDEQPPALPSLRVVMLSGDWIVPELPQRLRRFAPN
AHCVAMGGATEAAIWSNYWIADTALTGWCSVPYGVPLPNQQFRIVNEQEEDCPDWVAGELWIGGQGVAQGYYGDSAGTEQ
QFILRDGQRWYRTGDTGRYRPDAIIEFLGRKDQQVKISGYRVELEEITLALKSYPSIEDVVAFVIQHNDRSVLAAVAVTP
TPLDRQAVTAFLRERLPEYAIPSRLGHCCAWPLTDNGKRDQHALRAFVTPSFEPTETPQVTQPMTAMEQLLCEQLQLLLN
VVSIQSHDNFFALGGDSFIAIRLTSVLRRNYGVELPLWKIFSVQTIAQIALVMEPAHQSAGRIQFVEDSI
>Mature_550_residues
MRESAHVGVALPRGCDQIIAVLAIQWLGAAYVPISVEWPACRRSQVITLADIHFLIGDRTLGWPEEVDVLSVESEPVSDE
RPTPRVVSADSLAYLIFTSGSTGVPKGVAVSHGAAVNTIESVNRQHQINPQDTALALSALYFDLSVWDVFGVLSAGARLV
LIPQQAQREAAIWLSLVQQHQVTVWNSVPALLEMMLLFNEQIFNEQDEQPPALPSLRVVMLSGDWIVPELPQRLRRFAPN
AHCVAMGGATEAAIWSNYWIADTALTGWCSVPYGVPLPNQQFRIVNEQEEDCPDWVAGELWIGGQGVAQGYYGDSAGTEQ
QFILRDGQRWYRTGDTGRYRPDAIIEFLGRKDQQVKISGYRVELEEITLALKSYPSIEDVVAFVIQHNDRSVLAAVAVTP
TPLDRQAVTAFLRERLPEYAIPSRLGHCCAWPLTDNGKRDQHALRAFVTPSFEPTETPQVTQPMTAMEQLLCEQLQLLLN
VVSIQSHDNFFALGGDSFIAIRLTSVLRRNYGVELPLWKIFSVQTIAQIALVMEPAHQSAGRIQFVEDSI

Specific function: According to Ref.3:an enzyme involved in the biosynthesis of anguibactin; an iron-binding siderophore [H]

COG id: COG1020

COG function: function code Q; Non-ribosomal peptide synthetase modules and related proteins

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 acyl carrier domain [H]

Homologues:

Organism=Homo sapiens, GI45580730, Length=473, Percent_Identity=26.6384778012685, Blast_Score=106, Evalue=7e-23,
Organism=Homo sapiens, GI4758332, Length=367, Percent_Identity=25.3405994550409, Blast_Score=90, Evalue=6e-18,
Organism=Homo sapiens, GI12669909, Length=367, Percent_Identity=25.3405994550409, Blast_Score=90, Evalue=6e-18,
Organism=Homo sapiens, GI42794754, Length=362, Percent_Identity=27.0718232044199, Blast_Score=88, Evalue=2e-17,
Organism=Homo sapiens, GI42794752, Length=362, Percent_Identity=27.0718232044199, Blast_Score=88, Evalue=2e-17,
Organism=Homo sapiens, GI187761345, Length=415, Percent_Identity=26.7469879518072, Blast_Score=79, Evalue=8e-15,
Organism=Homo sapiens, GI187761343, Length=415, Percent_Identity=26.7469879518072, Blast_Score=79, Evalue=8e-15,
Organism=Homo sapiens, GI156151445, Length=360, Percent_Identity=23.8888888888889, Blast_Score=76, Evalue=7e-14,
Organism=Escherichia coli, GI1786801, Length=560, Percent_Identity=30.5357142857143, Blast_Score=202, Evalue=5e-53,
Organism=Escherichia coli, GI1786810, Length=379, Percent_Identity=25.5936675461741, Blast_Score=81, Evalue=2e-16,
Organism=Escherichia coli, GI1788107, Length=372, Percent_Identity=23.3870967741935, Blast_Score=77, Evalue=4e-15,
Organism=Escherichia coli, GI145693145, Length=371, Percent_Identity=23.989218328841, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI1790505, Length=420, Percent_Identity=23.8095238095238, Blast_Score=67, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17556356, Length=529, Percent_Identity=26.2759924385633, Blast_Score=111, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI17550940, Length=538, Percent_Identity=23.4200743494424, Blast_Score=88, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI32564420, Length=455, Percent_Identity=22.4175824175824, Blast_Score=84, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI32564422, Length=455, Percent_Identity=22.4175824175824, Blast_Score=84, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17538037, Length=378, Percent_Identity=23.5449735449735, Blast_Score=70, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6319591, Length=644, Percent_Identity=26.5527950310559, Blast_Score=153, Evalue=6e-38,
Organism=Saccharomyces cerevisiae, GI6319699, Length=363, Percent_Identity=22.5895316804408, Blast_Score=88, Evalue=4e-18,
Organism=Drosophila melanogaster, GI24648676, Length=580, Percent_Identity=25.1724137931034, Blast_Score=126, Evalue=5e-29,
Organism=Drosophila melanogaster, GI18859661, Length=350, Percent_Identity=24.2857142857143, Blast_Score=83, Evalue=4e-16,
Organism=Drosophila melanogaster, GI62471681, Length=351, Percent_Identity=24.2165242165242, Blast_Score=83, Evalue=5e-16,
Organism=Drosophila melanogaster, GI62471687, Length=351, Percent_Identity=24.2165242165242, Blast_Score=83, Evalue=5e-16,
Organism=Drosophila melanogaster, GI24586634, Length=351, Percent_Identity=24.2165242165242, Blast_Score=83, Evalue=5e-16,
Organism=Drosophila melanogaster, GI22026970, Length=351, Percent_Identity=24.2165242165242, Blast_Score=83, Evalue=5e-16,
Organism=Drosophila melanogaster, GI62471679, Length=351, Percent_Identity=24.2165242165242, Blast_Score=83, Evalue=5e-16,
Organism=Drosophila melanogaster, GI62471683, Length=351, Percent_Identity=24.2165242165242, Blast_Score=83, Evalue=5e-16,
Organism=Drosophila melanogaster, GI62471685, Length=351, Percent_Identity=24.2165242165242, Blast_Score=83, Evalue=5e-16,
Organism=Drosophila melanogaster, GI24586636, Length=351, Percent_Identity=24.2165242165242, Blast_Score=83, Evalue=5e-16,
Organism=Drosophila melanogaster, GI62471689, Length=351, Percent_Identity=24.2165242165242, Blast_Score=83, Evalue=5e-16,
Organism=Drosophila melanogaster, GI21356947, Length=366, Percent_Identity=23.7704918032787, Blast_Score=69, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010071
- InterPro:   IPR009081
- InterPro:   IPR020845
- InterPro:   IPR000873
- InterPro:   IPR001242
- InterPro:   IPR006163 [H]

Pfam domain/function: PF00501 AMP-binding; PF00668 Condensation; PF00550 PP-binding [H]

EC number: 2.7.7.- [C]

Molecular weight: Translated: 61040; Mature: 61040

Theoretical pI: Translated: 4.67; Mature: 4.67

Prosite motif: PS50075 ACP_DOMAIN ; PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRESAHVGVALPRGCDQIIAVLAIQWLGAAYVPISVEWPACRRSQVITLADIHFLIGDRT
CCCCCCCCEECCCCHHHHHHHHHHHHHCCEEEEEEECCCCCCCCCEEEEEEEEEEECCCC
LGWPEEVDVLSVESEPVSDERPTPRVVSADSLAYLIFTSGSTGVPKGVAVSHGAAVNTIE
CCCCCCCEEEEECCCCCCCCCCCCCEEECCCEEEEEEECCCCCCCCCEEECCCCHHHHHH
SVNRQHQINPQDTALALSALYFDLSVWDVFGVLSAGARLVLIPQQAQREAAIWLSLVQQH
HHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHHC
QVTVWNSVPALLEMMLLFNEQIFNEQDEQPPALPSLRVVMLSGDWIVPELPQRLRRFAPN
CEEEEHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHCCC
AHCVAMGGATEAAIWSNYWIADTALTGWCSVPYGVPLPNQQFRIVNEQEEDCPDWVAGEL
CEEEEECCCCCHHHHCCCEEECCCCCCEECCCCCCCCCCCCEEEECCCCCCCCCHHCCEE
WIGGQGVAQGYYGDSAGTEQQFILRDGQRWYRTGDTGRYRPDAIIEFLGRKDQQVKISGY
EECCCCCCCCCCCCCCCCCCHHEECCCCCEEECCCCCCCCHHHHHHHHCCCCCEEEEEEE
RVELEEITLALKSYPSIEDVVAFVIQHNDRSVLAAVAVTPTPLDRQAVTAFLRERLPEYA
EEEHHHHHHHHHCCCCHHHHHHHHHCCCCCEEEEEEEECCCCCCHHHHHHHHHHHCCCCC
IPSRLGHCCAWPLTDNGKRDQHALRAFVTPSFEPTETPQVTQPMTAMEQLLCEQLQLLLN
CHHHHCCEEECCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
VVSIQSHDNFFALGGDSFIAIRLTSVLRRNYGVELPLWKIFSVQTIAQIALVMEPAHQSA
HHHHCCCCCEEEECCCCEEEHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCC
GRIQFVEDSI
CCEEEECCCC
>Mature Secondary Structure
MRESAHVGVALPRGCDQIIAVLAIQWLGAAYVPISVEWPACRRSQVITLADIHFLIGDRT
CCCCCCCCEECCCCHHHHHHHHHHHHHCCEEEEEEECCCCCCCCCEEEEEEEEEEECCCC
LGWPEEVDVLSVESEPVSDERPTPRVVSADSLAYLIFTSGSTGVPKGVAVSHGAAVNTIE
CCCCCCCEEEEECCCCCCCCCCCCCEEECCCEEEEEEECCCCCCCCCEEECCCCHHHHHH
SVNRQHQINPQDTALALSALYFDLSVWDVFGVLSAGARLVLIPQQAQREAAIWLSLVQQH
HHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHHC
QVTVWNSVPALLEMMLLFNEQIFNEQDEQPPALPSLRVVMLSGDWIVPELPQRLRRFAPN
CEEEEHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHCCC
AHCVAMGGATEAAIWSNYWIADTALTGWCSVPYGVPLPNQQFRIVNEQEEDCPDWVAGEL
CEEEEECCCCCHHHHCCCEEECCCCCCEECCCCCCCCCCCCEEEECCCCCCCCCHHCCEE
WIGGQGVAQGYYGDSAGTEQQFILRDGQRWYRTGDTGRYRPDAIIEFLGRKDQQVKISGY
EECCCCCCCCCCCCCCCCCCHHEECCCCCEEECCCCCCCCHHHHHHHHCCCCCEEEEEEE
RVELEEITLALKSYPSIEDVVAFVIQHNDRSVLAAVAVTPTPLDRQAVTAFLRERLPEYA
EEEHHHHHHHHHCCCCHHHHHHHHHCCCCCEEEEEEEECCCCCCHHHHHHHHHHHCCCCC
IPSRLGHCCAWPLTDNGKRDQHALRAFVTPSFEPTETPQVTQPMTAMEQLLCEQLQLLLN
CHHHHCCEEECCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
VVSIQSHDNFFALGGDSFIAIRLTSVLRRNYGVELPLWKIFSVQTIAQIALVMEPAHQSA
HHHHCCCCCEEEECCCCEEEHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCC
GRIQFVEDSI
CCEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Phosphopantetheine. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 6 ATP; L-serine; 2,3-dihydroxybenzoate [C]

Specific reaction: 6 ATP + 3 L-serine + 3 2,3-dihydroxybenzoate = 6 pyrophosphate + 6 AMP + enterobactin ATP + L-serine = pyrophosphate + L-Seryl-AMP 6 ATP + 3 L-serine + 3 2,3-dihydroxybenzoate = 6 pyrophosphate + 6 AMP + enterobactin ATP + L-serine = pyrophosphate + L-Ser

General reaction: Transferases; Acyltransferases; Transferring groups other than amino-acyl groups [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2311935; 8335354 [H]