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The map label for this gene is papD [H]

Identifier: 218927888

GI number: 218927888

Start: 760928

End: 761647

Strand: Direct

Name: papD [H]

Synonym: YPO0699

Alternate gene names: 218927888

Gene position: 760928-761647 (Clockwise)

Preceding gene: 218927887

Following gene: 218927889

Centisome position: 16.35

GC content: 48.89

Gene sequence:

>720_bases
ATGAAGAAGCTCTCATTAATCGCGTTGACCAGCACAGTGGTATTCACCAGTTATACCGCCAATGCTGCGATTGCATTAGA
CCGGACGCGAATTATTTATAACGGGCAGGATAAGTCCATTAGCCTGAATATCAGTAATGAAAATAAAACACTGCCCTATT
TAGCACAGGCGTGGATTGAGAATGAACAACAGCAAAAAGTCACCACCCCGTTAGTGGCAACGCCACCAGTTCAGCGAGTA
GAAGCGGGTAAGAAAAGCGCGGTGCGTATCTCTGCCACCCCTGAGGCGCTGAAATTGCCACAAGATCGCGAGTCACTGTT
CTACTTCAATCTGCGTGAAATTCCGCCTCGCAGTGAAAAATCTAATGTCATGCAGATTGCCTTACAAACCAAAATCAAAC
TGTTCTACCGGCCAGAAGGGATTACCCCTGCTACGGGCCAAATCTGGCAGGAACAACTGGTTCTGCATAAAACGGCGCAA
GGTTTTCGGCTAGAGAACCCCACTCCCTATTACATTACGGTCGTTGGGCTGGCGGAGAAAGGTAAGTCATCGGCTGACAG
CAATTTTCAACCTTTGATGGTTGCGCCCAAATCCAGTGCTGAGCTGAAATCTGCCAGTTTCACCGCCCCCGTCCTGACCT
ACATTAACGATTATGGTGGCCGACCAGAACTGAGTTTCAGTTGCAGCGGCAGTACTTGCCGGGCAGCTAAAGCGATTTAA

Upstream 100 bases:

>100_bases
GTGCTAAGCAGTGCCAGGTGGCGATCCCAGCAGTAATACAGCCGCAGGCGCAAATATTATTACCCTGCATATCCGTTAAC
TAATATTGAGGTGATATACC

Downstream 100 bases:

>100_bases
ACCGGTTGAAAGAGAGAACCATCAATGAAAAAAAATATATTCCTCACGTTACGCAGTGAGAGGTATAAGGCGGGACTGCT
GTTGTTGCTGCTACTCCTGT

Product: fimbrial chaperone protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 239; Mature: 239

Protein sequence:

>239_residues
MKKLSLIALTSTVVFTSYTANAAIALDRTRIIYNGQDKSISLNISNENKTLPYLAQAWIENEQQQKVTTPLVATPPVQRV
EAGKKSAVRISATPEALKLPQDRESLFYFNLREIPPRSEKSNVMQIALQTKIKLFYRPEGITPATGQIWQEQLVLHKTAQ
GFRLENPTPYYITVVGLAEKGKSSADSNFQPLMVAPKSSAELKSASFTAPVLTYINDYGGRPELSFSCSGSTCRAAKAI

Sequences:

>Translated_239_residues
MKKLSLIALTSTVVFTSYTANAAIALDRTRIIYNGQDKSISLNISNENKTLPYLAQAWIENEQQQKVTTPLVATPPVQRV
EAGKKSAVRISATPEALKLPQDRESLFYFNLREIPPRSEKSNVMQIALQTKIKLFYRPEGITPATGQIWQEQLVLHKTAQ
GFRLENPTPYYITVVGLAEKGKSSADSNFQPLMVAPKSSAELKSASFTAPVLTYINDYGGRPELSFSCSGSTCRAAKAI
>Mature_239_residues
MKKLSLIALTSTVVFTSYTANAAIALDRTRIIYNGQDKSISLNISNENKTLPYLAQAWIENEQQQKVTTPLVATPPVQRV
EAGKKSAVRISATPEALKLPQDRESLFYFNLREIPPRSEKSNVMQIALQTKIKLFYRPEGITPATGQIWQEQLVLHKTAQ
GFRLENPTPYYITVVGLAEKGKSSADSNFQPLMVAPKSSAELKSASFTAPVLTYINDYGGRPELSFSCSGSTCRAAKAI

Specific function: Binds and caps interactive surfaces on pilus subunits to prevent them from participating in non-productive interactions. Facilitates the import of subunits into the periplasm. May facilitate subunit folding. Chaperone-subunit complexes are then targeted t

COG id: COG3121

COG function: function code NU; P pilus assembly protein, chaperone PapD

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Ig-like (immunoglobulin-like) domain [H]

Homologues:

Organism=Escherichia coli, GI1788677, Length=210, Percent_Identity=45.2380952380952, Blast_Score=218, Evalue=2e-58,
Organism=Escherichia coli, GI1786936, Length=241, Percent_Identity=40.6639004149378, Blast_Score=177, Evalue=6e-46,
Organism=Escherichia coli, GI87082203, Length=228, Percent_Identity=36.4035087719298, Blast_Score=147, Evalue=6e-37,
Organism=Escherichia coli, GI1789532, Length=205, Percent_Identity=33.6585365853659, Blast_Score=120, Evalue=9e-29,
Organism=Escherichia coli, GI1786333, Length=246, Percent_Identity=32.1138211382114, Blast_Score=117, Evalue=5e-28,
Organism=Escherichia coli, GI1788428, Length=230, Percent_Identity=30.8695652173913, Blast_Score=117, Evalue=6e-28,
Organism=Escherichia coli, GI1790771, Length=206, Percent_Identity=34.9514563106796, Blast_Score=108, Evalue=4e-25,
Organism=Escherichia coli, GI1787171, Length=212, Percent_Identity=29.7169811320755, Blast_Score=96, Evalue=2e-21,
Organism=Escherichia coli, GI1786743, Length=229, Percent_Identity=30.5676855895196, Blast_Score=91, Evalue=8e-20,
Organism=Escherichia coli, GI87081806, Length=232, Percent_Identity=28.0172413793103, Blast_Score=81, Evalue=7e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008962
- InterPro:   IPR001829
- InterPro:   IPR016148
- InterPro:   IPR018046
- InterPro:   IPR016147 [H]

Pfam domain/function: PF02753 Pili_assembly_C; PF00345 Pili_assembly_N [H]

EC number: NA

Molecular weight: Translated: 26358; Mature: 26358

Theoretical pI: Translated: 9.98; Mature: 9.98

Prosite motif: PS00635 PILI_CHAPERONE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKLSLIALTSTVVFTSYTANAAIALDRTRIIYNGQDKSISLNISNENKTLPYLAQAWIE
CCCEEEEEEEEEEEEEEECCCEEEEEECEEEEECCCCCEEEEEECCCCCCCHHHHHHHHC
NEQQQKVTTPLVATPPVQRVEAGKKSAVRISATPEALKLPQDRESLFYFNLREIPPRSEK
CCCHHHHCCCEEECCCHHHHHCCCCCEEEEECCCHHHCCCCCCCEEEEEEEECCCCCCCC
SNVMQIALQTKIKLFYRPEGITPATGQIWQEQLVLHKTAQGFRLENPTPYYITVVGLAEK
CCEEEEEEEEEEEEEECCCCCCCCCHHHHHHHHHHHHCCCCEECCCCCCEEEEEEEECCC
GKSSADSNFQPLMVAPKSSAELKSASFTAPVLTYINDYGGRPELSFSCSGSTCRAAKAI
CCCCCCCCCCEEEEECCCCCCCCCCCCCHHHHHHHHCCCCCCCEEEEECCCCCCCCCCC
>Mature Secondary Structure
MKKLSLIALTSTVVFTSYTANAAIALDRTRIIYNGQDKSISLNISNENKTLPYLAQAWIE
CCCEEEEEEEEEEEEEEECCCEEEEEECEEEEECCCCCEEEEEECCCCCCCHHHHHHHHC
NEQQQKVTTPLVATPPVQRVEAGKKSAVRISATPEALKLPQDRESLFYFNLREIPPRSEK
CCCHHHHCCCEEECCCHHHHHCCCCCEEEEECCCHHHCCCCCCCEEEEEEEECCCCCCCC
SNVMQIALQTKIKLFYRPEGITPATGQIWQEQLVLHKTAQGFRLENPTPYYITVVGLAEK
CCEEEEEEEEEEEEEECCCCCCCCCHHHHHHHHHHHHCCCCEECCCCCCEEEEEEEECCC
GKSSADSNFQPLMVAPKSSAELKSASFTAPVLTYINDYGGRPELSFSCSGSTCRAAKAI
CCCCCCCCCCEEEEECCCCCCCCCCCCCHHHHHHHHCCCCCCCEEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1357526; 10049807; 2478891; 10446050 [H]