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The map label for this gene is folA [H]

Identifier: 218927684

GI number: 218927684

Start: 519099

End: 519581

Strand: Direct

Name: folA [H]

Synonym: YPO0486

Alternate gene names: 218927684

Gene position: 519099-519581 (Clockwise)

Preceding gene: 229220641

Following gene: 218927685

Centisome position: 11.15

GC content: 48.65

Gene sequence:

>483_bases
ATGATTATCAGCCTGATCGCTGCATTGGCAGCAGATCGCGTTATTGGCATGGAAAACGCTATGCCATGGCATTTACCGGC
TGATTTAGCGTGGTTCAAACGTAACACGTTAAATAAACCGGTAATTATGGGTCGTAAGACCTTCGAATCTATCGGTCGCC
CCCTTCCGGGAAGACTGAATATTGTCATCAGTAGCCAGCCTGGTACTGATGAACGTGTGACGTGGGCGGCCTCGATTGAA
GAAGCATTGGCGTTTGCTGGCAATGCGGAAGAAGTGATGGTCATGGGCGGTGGCCGTGTCTATAAGCAATTCTTAGACCG
CGCAAATCGCATGTATCTCACGCATATTGATGCGGAGGTCGGTGGCGATACTCACTTCCCTGATTATGAGCCAGATGAAT
GGGAAAGCGTATTCAGTGAGTTTCATGATGCCGATGAAGCTAACTCACACAGTTATTGCTTCGAAATTCTAGAGCGCCGC
TAA

Upstream 100 bases:

>100_bases
GTATCATTCGCTTACTGATCGCTATCGACGCTTGATTAGGCTTTACGTATAGTGTGAACAATTTTTCGGGACTGGCTCTT
GCAGCCTACAGGGTTTAATA

Downstream 100 bases:

>100_bases
GAATATATATATTCACATCAAAACAATTAGCATACAGGCCCATTAGGTAATAATGGGCCTGTTTCATTGTGCCCAGAAAA
CCCCCAGCTAGGCTGGGGGT

Product: dihydrofolate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 160; Mature: 160

Protein sequence:

>160_residues
MIISLIAALAADRVIGMENAMPWHLPADLAWFKRNTLNKPVIMGRKTFESIGRPLPGRLNIVISSQPGTDERVTWAASIE
EALAFAGNAEEVMVMGGGRVYKQFLDRANRMYLTHIDAEVGGDTHFPDYEPDEWESVFSEFHDADEANSHSYCFEILERR

Sequences:

>Translated_160_residues
MIISLIAALAADRVIGMENAMPWHLPADLAWFKRNTLNKPVIMGRKTFESIGRPLPGRLNIVISSQPGTDERVTWAASIE
EALAFAGNAEEVMVMGGGRVYKQFLDRANRMYLTHIDAEVGGDTHFPDYEPDEWESVFSEFHDADEANSHSYCFEILERR
>Mature_160_residues
MIISLIAALAADRVIGMENAMPWHLPADLAWFKRNTLNKPVIMGRKTFESIGRPLPGRLNIVISSQPGTDERVTWAASIE
EALAFAGNAEEVMVMGGGRVYKQFLDRANRMYLTHIDAEVGGDTHFPDYEPDEWESVFSEFHDADEANSHSYCFEILERR

Specific function: Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis [H]

COG id: COG0262

COG function: function code H; Dihydrofolate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 DHFR (dihydrofolate reductase) domain [H]

Homologues:

Organism=Escherichia coli, GI1786233, Length=159, Percent_Identity=80.5031446540881, Blast_Score=279, Evalue=7e-77,
Organism=Saccharomyces cerevisiae, GI6324810, Length=162, Percent_Identity=32.7160493827161, Blast_Score=70, Evalue=1e-13,
Organism=Drosophila melanogaster, GI116008026, Length=177, Percent_Identity=29.3785310734463, Blast_Score=72, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24647458, Length=177, Percent_Identity=29.3785310734463, Blast_Score=72, Evalue=1e-13,

Paralogues:

None

Copy number: 640 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 10,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012259
- InterPro:   IPR017925
- InterPro:   IPR001796 [H]

Pfam domain/function: PF00186 DHFR_1 [H]

EC number: =1.5.1.3 [H]

Molecular weight: Translated: 18059; Mature: 18059

Theoretical pI: Translated: 4.63; Mature: 4.63

Prosite motif: PS00075 DHFR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIISLIAALAADRVIGMENAMPWHLPADLAWFKRNTLNKPVIMGRKTFESIGRPLPGRLN
CHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHCCCCCCEEECHHHHHHHCCCCCCEEE
IVISSQPGTDERVTWAASIEEALAFAGNAEEVMVMGGGRVYKQFLDRANRMYLTHIDAEV
EEEECCCCCCCCEEHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHCCCEEEEEECCCC
GGDTHFPDYEPDEWESVFSEFHDADEANSHSYCFEILERR
CCCCCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHCC
>Mature Secondary Structure
MIISLIAALAADRVIGMENAMPWHLPADLAWFKRNTLNKPVIMGRKTFESIGRPLPGRLN
CHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHCCCCCCEEECHHHHHHHCCCCCCEEE
IVISSQPGTDERVTWAASIEEALAFAGNAEEVMVMGGGRVYKQFLDRANRMYLTHIDAEV
EEEECCCCCCCCEEHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHCCCEEEEEECCCC
GGDTHFPDYEPDEWESVFSEFHDADEANSHSYCFEILERR
CCCCCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1766362 [H]