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The map label for this gene is carA

Identifier: 218927679

GI number: 218927679

Start: 512107

End: 513282

Strand: Direct

Name: carA

Synonym: YPO0481

Alternate gene names: 218927679

Gene position: 512107-513282 (Clockwise)

Preceding gene: 218927678

Following gene: 218927680

Centisome position: 11.0

GC content: 50.6

Gene sequence:

>1176_bases
TTGATTAAGTCAGCGCTATTGGTTCTCGAAGACGGAACCCAATTCCACGGTCGGGCCATCGGGGCAGAAGGTACGGCAGT
AGGGGAAGTGGTCTTCAATACGTCGATGACCGGTTATCAAGAAATCCTCACTGATCCTTCCTACTCCCGCCAGATCGTTA
CTCTTACTTATCCTCATATCGGCAATGTCGGCACTAATGCCTCCGATGAAGAATCCTCTGCAGTACACGCCCAAGGTCTT
GTTATTCGCGACCTGCCATTGATTGCCAGCAACTACCGTAATGAAGAAGGCTTATCCGAGTATCTCAAGCGGCACAATAT
TGTGGCGATTGCCGATATCGATACTCGTAAGCTGACTCGCTTGCTGCGCGAAAAAGGAGCACAGAACGGCTGCATTATTG
TTGGGGAGTTGTCTGACGCGGCCCTGGCATTGGAAAAAGCGAAAGCATTTCCAGGCTTGAAAGGGATGGATCTGGCGAAA
GAAGTGACCACCAAAGAGATGTATCAGTGGCTGCAAGGCAGTTGGACGCTGGAAGGTGATTTACCTGCGGCGAAACAACC
AGAAGATTTGCCATTCCATGTGGTGGCTTACGATTATGGTGTGAAGCGTAATATCCTGCGTATGCTGGTTGATCGCGGTT
GCCGTCTGACCGTTGTTCCAGCGCAGACACCCGCTGAAGACGTTCTGAAACTGAATCCAGATGGCATCTTCTTATCCAAT
GGTCCTGGGGATCCAGAACCATGCGATTACGCGATCACGGCCATCAAACGCTTCCTGGAAACGGATATTCCGGTATTCGG
CATCTGTCTGGGCCACCAATTGCTGGCGCTGGCCAGTGGCGCTAAAACAGTAAAAATGAAGTTCGGTCACCATGGGGGTA
ACCATCCGGTGAAAGATCTGGATGCTAGCTGTGTGATGATTACCGCGCAAAACCATGGCTTCGCCGTTGATGAAACCTCA
TTGCCATCCAACCTGCGTACTACACATGTCTCTTTATTTGATGGTTCTCTGCAAGGGCTCCACCGTACAGATAAAGCAGC
GTTCAGCTTCCAGGGGCATCCAGAAGCCAGCCCCGGCCCGCATGATGCCGCGCCATTGTTTGATCATTTTATCGAACTGA
TTGAGGCTTACCGAGCTTCATCCGTGAGTCTTAACTGTAGTAACAGCCATAAATAA

Upstream 100 bases:

>100_bases
TGCCAAAAATTAGCTTTGAGAGCGGTTTTTGCATTGATTTAGATCGCTAGATATGAATTAATATGCAGATAATGTGACTG
TTTATTCCCTGGAGGATGTT

Downstream 100 bases:

>100_bases
TCAGGAGCGAATACCATGCCAAAACGTACAGATATAAAAAGCATCCTGATTCTGGGCGCAGGCCCGATTGTTATCGGCCA
GGCTTGTGAGTTTGACTACT

Product: carbamoyl phosphate synthase small subunit

Products: NA

Alternate protein names: Carbamoyl-phosphate synthetase glutamine chain

Number of amino acids: Translated: 391; Mature: 391

Protein sequence:

>391_residues
MIKSALLVLEDGTQFHGRAIGAEGTAVGEVVFNTSMTGYQEILTDPSYSRQIVTLTYPHIGNVGTNASDEESSAVHAQGL
VIRDLPLIASNYRNEEGLSEYLKRHNIVAIADIDTRKLTRLLREKGAQNGCIIVGELSDAALALEKAKAFPGLKGMDLAK
EVTTKEMYQWLQGSWTLEGDLPAAKQPEDLPFHVVAYDYGVKRNILRMLVDRGCRLTVVPAQTPAEDVLKLNPDGIFLSN
GPGDPEPCDYAITAIKRFLETDIPVFGICLGHQLLALASGAKTVKMKFGHHGGNHPVKDLDASCVMITAQNHGFAVDETS
LPSNLRTTHVSLFDGSLQGLHRTDKAAFSFQGHPEASPGPHDAAPLFDHFIELIEAYRASSVSLNCSNSHK

Sequences:

>Translated_391_residues
MIKSALLVLEDGTQFHGRAIGAEGTAVGEVVFNTSMTGYQEILTDPSYSRQIVTLTYPHIGNVGTNASDEESSAVHAQGL
VIRDLPLIASNYRNEEGLSEYLKRHNIVAIADIDTRKLTRLLREKGAQNGCIIVGELSDAALALEKAKAFPGLKGMDLAK
EVTTKEMYQWLQGSWTLEGDLPAAKQPEDLPFHVVAYDYGVKRNILRMLVDRGCRLTVVPAQTPAEDVLKLNPDGIFLSN
GPGDPEPCDYAITAIKRFLETDIPVFGICLGHQLLALASGAKTVKMKFGHHGGNHPVKDLDASCVMITAQNHGFAVDETS
LPSNLRTTHVSLFDGSLQGLHRTDKAAFSFQGHPEASPGPHDAAPLFDHFIELIEAYRASSVSLNCSNSHK
>Mature_391_residues
MIKSALLVLEDGTQFHGRAIGAEGTAVGEVVFNTSMTGYQEILTDPSYSRQIVTLTYPHIGNVGTNASDEESSAVHAQGL
VIRDLPLIASNYRNEEGLSEYLKRHNIVAIADIDTRKLTRLLREKGAQNGCIIVGELSDAALALEKAKAFPGLKGMDLAK
EVTTKEMYQWLQGSWTLEGDLPAAKQPEDLPFHVVAYDYGVKRNILRMLVDRGCRLTVVPAQTPAEDVLKLNPDGIFLSN
GPGDPEPCDYAITAIKRFLETDIPVFGICLGHQLLALASGAKTVKMKFGHHGGNHPVKDLDASCVMITAQNHGFAVDETS
LPSNLRTTHVSLFDGSLQGLHRTDKAAFSFQGHPEASPGPHDAAPLFDHFIELIEAYRASSVSLNCSNSHK

Specific function: Arginine biosynthesis. Pyrimidine biosynthesis; first step. [C]

COG id: COG0505

COG function: function code EF; Carbamoylphosphate synthase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain

Homologues:

Organism=Homo sapiens, GI18105007, Length=382, Percent_Identity=38.4816753926702, Blast_Score=232, Evalue=4e-61,
Organism=Homo sapiens, GI169790915, Length=394, Percent_Identity=35.5329949238579, Blast_Score=229, Evalue=4e-60,
Organism=Homo sapiens, GI21361331, Length=394, Percent_Identity=35.5329949238579, Blast_Score=229, Evalue=4e-60,
Organism=Escherichia coli, GI1786215, Length=381, Percent_Identity=86.6141732283465, Blast_Score=696, Evalue=0.0,
Organism=Caenorhabditis elegans, GI193204318, Length=390, Percent_Identity=35.1282051282051, Blast_Score=227, Evalue=7e-60,
Organism=Saccharomyces cerevisiae, GI6324878, Length=405, Percent_Identity=38.7654320987654, Blast_Score=248, Evalue=1e-66,
Organism=Saccharomyces cerevisiae, GI6322331, Length=397, Percent_Identity=37.0277078085642, Blast_Score=232, Evalue=9e-62,
Organism=Drosophila melanogaster, GI45555749, Length=388, Percent_Identity=39.4329896907216, Blast_Score=234, Evalue=1e-61,
Organism=Drosophila melanogaster, GI24642586, Length=388, Percent_Identity=39.4329896907216, Blast_Score=233, Evalue=1e-61,

Paralogues:

None

Copy number: 620 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2599 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): CARA_YERPE (Q8ZIL5)

Other databases:

- EMBL:   AL590842
- EMBL:   AE009952
- EMBL:   AE017042
- PIR:   AG0059
- RefSeq:   NP_670990.1
- RefSeq:   NP_994969.1
- RefSeq:   YP_002345554.1
- ProteinModelPortal:   Q8ZIL5
- SMR:   Q8ZIL5
- IntAct:   Q8ZIL5
- GeneID:   1148640
- GeneID:   1173326
- GeneID:   2766699
- GenomeReviews:   AE009952_GR
- GenomeReviews:   AE017042_GR
- GenomeReviews:   AL590842_GR
- KEGG:   ype:YPO0481
- KEGG:   ypk:y3693
- KEGG:   ypm:YP_3698
- HOGENOM:   HBG286341
- OMA:   FTYPELG
- ProtClustDB:   PRK12564
- BioCyc:   YPES187410:Y3693-MONOMER
- BioCyc:   YPES214092:YPO0481-MONOMER
- BRENDA:   6.3.5.5
- HAMAP:   MF_01209_B
- InterPro:   IPR006220
- InterPro:   IPR001317
- InterPro:   IPR006274
- InterPro:   IPR002474
- InterPro:   IPR011702
- InterPro:   IPR017926
- InterPro:   IPR000991
- PANTHER:   PTHR11405:SF4
- PRINTS:   PR00097
- PRINTS:   PR00099
- PRINTS:   PR00096
- TIGRFAMs:   TIGR01368

Pfam domain/function: PF00988 CPSase_sm_chain; PF00117 GATase; SSF52021 CP_synthsmall

EC number: =6.3.5.5

Molecular weight: Translated: 42423; Mature: 42423

Theoretical pI: Translated: 6.08; Mature: 6.08

Prosite motif: PS51273 GATASE_TYPE_1; PS00442 GATASE_TYPE_I

Important sites: ACT_SITE 269-269 ACT_SITE 353-353 ACT_SITE 355-355

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKSALLVLEDGTQFHGRAIGAEGTAVGEVVFNTSMTGYQEILTDPSYSRQIVTLTYPHI
CCCCEEEEEECCCEECCEEECCCCCEEEHEEECCCCCHHHHHHCCCCCCCEEEEEECCCC
GNVGTNASDEESSAVHAQGLVIRDLPLIASNYRNEEGLSEYLKRHNIVAIADIDTRKLTR
CCCCCCCCCCCCCHHHHCCEEEECCCHHHHCCCCHHHHHHHHHHCCEEEEEECCHHHHHH
LLREKGAQNGCIIVGELSDAALALEKAKAFPGLKGMDLAKEVTTKEMYQWLQGSWTLEGD
HHHHCCCCCCEEEEECCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCEEEECC
LPAAKQPEDLPFHVVAYDYGVKRNILRMLVDRGCRLTVVPAQTPAEDVLKLNPDGIFLSN
CCCCCCCCCCCEEEEEECCCHHHHHHHHHHHCCCEEEEEECCCCHHHHEEECCCEEEECC
GPGDPEPCDYAITAIKRFLETDIPVFGICLGHQLLALASGAKTVKMKFGHHGGNHPVKDL
CCCCCCCHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCC
DASCVMITAQNHGFAVDETSLPSNLRTTHVSLFDGSLQGLHRTDKAAFSFQGHPEASPGP
CCCEEEEEECCCCEEEECCCCCCCCCEEEEEEECCCHHHHHHCCCCEEEECCCCCCCCCC
HDAAPLFDHFIELIEAYRASSVSLNCSNSHK
CCCHHHHHHHHHHHHHHCCCCEEEECCCCCC
>Mature Secondary Structure
MIKSALLVLEDGTQFHGRAIGAEGTAVGEVVFNTSMTGYQEILTDPSYSRQIVTLTYPHI
CCCCEEEEEECCCEECCEEECCCCCEEEHEEECCCCCHHHHHHCCCCCCCEEEEEECCCC
GNVGTNASDEESSAVHAQGLVIRDLPLIASNYRNEEGLSEYLKRHNIVAIADIDTRKLTR
CCCCCCCCCCCCCHHHHCCEEEECCCHHHHCCCCHHHHHHHHHHCCEEEEEECCHHHHHH
LLREKGAQNGCIIVGELSDAALALEKAKAFPGLKGMDLAKEVTTKEMYQWLQGSWTLEGD
HHHHCCCCCCEEEEECCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCEEEECC
LPAAKQPEDLPFHVVAYDYGVKRNILRMLVDRGCRLTVVPAQTPAEDVLKLNPDGIFLSN
CCCCCCCCCCCEEEEEECCCHHHHHHHHHHHCCCEEEEEECCCCHHHHEEECCCEEEECC
GPGDPEPCDYAITAIKRFLETDIPVFGICLGHQLLALASGAKTVKMKFGHHGGNHPVKDL
CCCCCCCHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCC
DASCVMITAQNHGFAVDETSLPSNLRTTHVSLFDGSLQGLHRTDKAAFSFQGHPEASPGP
CCCEEEEEECCCCEEEECCCCCCCCCEEEEEEECCCHHHHHHCCCCEEEECCCCCCCCCC
HDAAPLFDHFIELIEAYRASSVSLNCSNSHK
CCCHHHHHHHHHHHHHHCCCCEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11586360; 12142430