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The map label for this gene is miaA [H]

Identifier: 218927575

GI number: 218927575

Start: 386887

End: 387828

Strand: Direct

Name: miaA [H]

Synonym: YPO0372

Alternate gene names: 218927575

Gene position: 386887-387828 (Clockwise)

Preceding gene: 218927574

Following gene: 218927576

Centisome position: 8.31

GC content: 51.17

Gene sequence:

>942_bases
ATGAATGATATTGAGAATCTAGATCGTCCACCGGCAATTTTTATTATGGGGCCAACTGCTTCCGGTAAAACCGCGCTCTC
TATTGCGTTGAGGCAGCGATTGCCTGTGGAGTTAGTCAGCGTCGATTCTGCTCTTATCTACCGTGGCATGGATATCGGTA
CCGCGAAACCCAGTGCACAAGAGCTGGCGTTAGCGCCACACCGGTTGATTGATATCCGTGATCCCGCTGAATCATACTCC
GCTGCCGATTTTCGTAAAGATGCTCTGAAAGAAATGGCCGACATCACTGCTGCGGGGCGTATTCCGCTGCTAGTGGGGGG
GACAATGTTGTATTTTAAAGCGCTGTTGGATGGGTTATCTCCGTTACCCTCCGCAGATCCTCAAGTACGCCAGCGTATTG
AACAGCAGGCGTCGGAGCTCGGTTGGGGCGCATTGCACCAGCAATTGGCCGTGATCGATCCAGTCGCGGCGGCACGGATT
CACCCCAATGATCCCCAAAGGCTCTCCAGAGCACTGGAAGTTTTTTTTATTTCAGGTAAAACTTTAACGGAACTGACTAA
AATTTCGGGTGAAACCTTACCTTACCGGGTTCACCAATTTGCGATTGCGCCTGCTAGCCGGGAACTGTTGCACCAGCGGA
TTGAATTACGTTTTCATCAGATGCTGGATGCAGGGTTTGAAGCAGAGGCTAGGGTGCTTTTTGACCGCGGTGATTTGCAT
ACGGACTTGCCTGCCATTCGTTGTGTGGGATACCGGCAGATGTGGTCTTATCTGTCTGGTGAGATTGATTACAACGACAT
GGTATACCGCGGAGTTTGTGCGACACGTCAACTGGCAAAGCGCCAAATGACCTGGTTGCGGGGCTGGAGTTCAGTTCAAT
GGCTCGACAGTGATAAGCCGGGAGAGGCTTTAGACTCCGTAATACAGGTTGTTAGTGCATAG

Upstream 100 bases:

>100_bases
TTGACGGAAGTTGAGCGTCTTTGTCCGCAGTTGGTGCAATCTCCTCCTGCTGGGCTATTACAACCTATTGATATAAAGGC
CGCATTGGCAACTTTGACAC

Downstream 100 bases:

>100_bases
GTTGAATGATTGTGTACAATTGATTAGTACTCAACGCACAGATTTTTTTACGCAGTTTATTTTCGAGCCGATAGGTTCTT
AGTTAAAAACAACAAGCAAA

Product: tRNA delta(2)-isopentenylpyrophosphate transferase

Products: NA

Alternate protein names: Dimethylallyl diphosphate:tRNA dimethylallyltransferase; DMAPP:tRNA dimethylallyltransferase; DMATase; Isopentenyl-diphosphate:tRNA isopentenyltransferase; IPP transferase; IPPT; IPTase [H]

Number of amino acids: Translated: 313; Mature: 313

Protein sequence:

>313_residues
MNDIENLDRPPAIFIMGPTASGKTALSIALRQRLPVELVSVDSALIYRGMDIGTAKPSAQELALAPHRLIDIRDPAESYS
AADFRKDALKEMADITAAGRIPLLVGGTMLYFKALLDGLSPLPSADPQVRQRIEQQASELGWGALHQQLAVIDPVAAARI
HPNDPQRLSRALEVFFISGKTLTELTKISGETLPYRVHQFAIAPASRELLHQRIELRFHQMLDAGFEAEARVLFDRGDLH
TDLPAIRCVGYRQMWSYLSGEIDYNDMVYRGVCATRQLAKRQMTWLRGWSSVQWLDSDKPGEALDSVIQVVSA

Sequences:

>Translated_313_residues
MNDIENLDRPPAIFIMGPTASGKTALSIALRQRLPVELVSVDSALIYRGMDIGTAKPSAQELALAPHRLIDIRDPAESYS
AADFRKDALKEMADITAAGRIPLLVGGTMLYFKALLDGLSPLPSADPQVRQRIEQQASELGWGALHQQLAVIDPVAAARI
HPNDPQRLSRALEVFFISGKTLTELTKISGETLPYRVHQFAIAPASRELLHQRIELRFHQMLDAGFEAEARVLFDRGDLH
TDLPAIRCVGYRQMWSYLSGEIDYNDMVYRGVCATRQLAKRQMTWLRGWSSVQWLDSDKPGEALDSVIQVVSA
>Mature_313_residues
MNDIENLDRPPAIFIMGPTASGKTALSIALRQRLPVELVSVDSALIYRGMDIGTAKPSAQELALAPHRLIDIRDPAESYS
AADFRKDALKEMADITAAGRIPLLVGGTMLYFKALLDGLSPLPSADPQVRQRIEQQASELGWGALHQQLAVIDPVAAARI
HPNDPQRLSRALEVFFISGKTLTELTKISGETLPYRVHQFAIAPASRELLHQRIELRFHQMLDAGFEAEARVLFDRGDLH
TDLPAIRCVGYRQMWSYLSGEIDYNDMVYRGVCATRQLAKRQMTWLRGWSSVQWLDSDKPGEALDSVIQVVSA

Specific function: Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i(6)A) [H]

COG id: COG0324

COG function: function code J; tRNA delta(2)-isopentenylpyrophosphate transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IPP transferase family [H]

Homologues:

Organism=Homo sapiens, GI31581534, Length=326, Percent_Identity=29.7546012269939, Blast_Score=127, Evalue=1e-29,
Organism=Escherichia coli, GI1790613, Length=313, Percent_Identity=80.1916932907348, Blast_Score=527, Evalue=1e-151,
Organism=Caenorhabditis elegans, GI25144712, Length=299, Percent_Identity=27.0903010033445, Blast_Score=105, Evalue=3e-23,
Organism=Saccharomyces cerevisiae, GI6324848, Length=288, Percent_Identity=28.8194444444444, Blast_Score=128, Evalue=1e-30,
Organism=Drosophila melanogaster, GI24649830, Length=267, Percent_Identity=31.0861423220974, Blast_Score=119, Evalue=3e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002627
- InterPro:   IPR018022 [H]

Pfam domain/function: PF01715 IPPT [H]

EC number: =2.5.1.75 [H]

Molecular weight: Translated: 34785; Mature: 34785

Theoretical pI: Translated: 6.41; Mature: 6.41

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNDIENLDRPPAIFIMGPTASGKTALSIALRQRLPVELVSVDSALIYRGMDIGTAKPSAQ
CCCCHHCCCCCEEEEECCCCCCCHHHHHHHHHCCCEEEEECCHHHHHCCCCCCCCCCCHH
ELALAPHRLIDIRDPAESYSAADFRKDALKEMADITAAGRIPLLVGGTMLYFKALLDGLS
HHHHCCHHEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECHHHHHHHHHHHCCC
PLPSADPQVRQRIEQQASELGWGALHQQLAVIDPVAAARIHPNDPQRLSRALEVFFISGK
CCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHCHHHHCCCCCCCHHHHHHHHHHHEECCC
TLTELTKISGETLPYRVHQFAIAPASRELLHQRIELRFHQMLDAGFEAEARVLFDRGDLH
HHHHHHHHCCCCCCEEHHHEEECCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCC
TDLPAIRCVGYRQMWSYLSGEIDYNDMVYRGVCATRQLAKRQMTWLRGWSSVQWLDSDKP
CCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCC
GEALDSVIQVVSA
HHHHHHHHHHHCC
>Mature Secondary Structure
MNDIENLDRPPAIFIMGPTASGKTALSIALRQRLPVELVSVDSALIYRGMDIGTAKPSAQ
CCCCHHCCCCCEEEEECCCCCCCHHHHHHHHHCCCEEEEECCHHHHHCCCCCCCCCCCHH
ELALAPHRLIDIRDPAESYSAADFRKDALKEMADITAAGRIPLLVGGTMLYFKALLDGLS
HHHHCCHHEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECHHHHHHHHHHHCCC
PLPSADPQVRQRIEQQASELGWGALHQQLAVIDPVAAARIHPNDPQRLSRALEVFFISGK
CCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHCHHHHCCCCCCCHHHHHHHHHHHEECCC
TLTELTKISGETLPYRVHQFAIAPASRELLHQRIELRFHQMLDAGFEAEARVLFDRGDLH
HHHHHHHHCCCCCCEEHHHEEECCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCC
TDLPAIRCVGYRQMWSYLSGEIDYNDMVYRGVCATRQLAKRQMTWLRGWSSVQWLDSDKP
CCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCC
GEALDSVIQVVSA
HHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA