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The map label for this gene is amiB [H]

Identifier: 218927573

GI number: 218927573

Start: 383190

End: 384971

Strand: Direct

Name: amiB [H]

Synonym: YPO0370

Alternate gene names: 218927573

Gene position: 383190-384971 (Clockwise)

Preceding gene: 218927572

Following gene: 218927574

Centisome position: 8.23

GC content: 50.84

Gene sequence:

>1782_bases
ATGATTATTGGTTTATTAACGCTCAGTTTATTCGCTCTGCCTCCCGCATTTGCCATGAAACTCACTGACATTAAGGTGAC
CAACGGGGCGAGTGAAAGTAAGGTTACTCTCAGCTTTGATGGTAAGCCAATTTATGCATTTTTCTCGCTAAACTCGCCGG
AGCGGGTGGTCTTGGATGTCCGCCAAAGTGGCAATATCTCAGGGTTGCCGCTGGAGTTCAGTGGGCAGAACCTGTTGAAA
CGAATTCGCTCCAGCACTCCGAAAGATGAACAAAGTACCCGCCTGGTACTGGAACTGACGCAGAAAGTAAAAACCCGTGC
TGTCACCCAACAGTCGGGCAATAACTACACGGTAGTGCTTACCATGACGGCCGTCGCCAGTGCCCCTGTTCGTCAGGCGC
AGGCCTCATTAAATCAGACGAATACCCCGAGTCCTAATGCCGGGCGTGTGGTGCCTCAAGTCACGAGCAAGGATTCAGTA
GCTAAAAATCCGTTTAATAATAAGCCCGTCGTCGTGGTCAGTAGCGAAAACGTGACCACGAATACTGCCCGTCCGATTAA
AACTGTGAGTGCGGCTAACAGTAGCCGTGTTGTGGTGGCCATAGATGCCGGTCATGGTGGTCAGGACCCAGGCGCTATCG
GGCAAAATGGTCTGAAAGAGAAAAATGTCACGATCTCCATTGCCCGCCGGCTTGAGGCACTGCTCAACCGGGATCCGATG
TTTAAACCGGTGCTGACCCGCAATGGTGATTACTTTATCTCGGTCATGGGGCGCTCTGATGTTGCACGTAAACAGGGGGC
TAATGTCTTGATTTCTATTCATGCTGATGCCGCGCCAAATCGCAGTGCGACAGGGGCATCGGTTTGGGTGTTATCAAACC
GCCGGGCTAATAGTGAAATGGGGAACTGGCTGGAGCAACATGAAAAGCAATCAGAACTGCTCGGGGGGGCTGGTGATGTA
CTGGCAAACACGGCTTCAGACCCTTATTTGAGTCAGGCGGTTCTGGATTTACAATTTGGTCACTCCCAGAGAGTGGGTTA
TGACGTCGCGACTAAAGTGCTGCGTGAGCTACAAACGGTGGGTGATATTCATAAACGCAAGCCGGAGCACGCCAGCCTTG
GGGTGTTACGTTCACCGGATATTCCGTCGCTATTAGTGGAAACCGGCTTTATCAGTAATAGTACGGAGGAACGGCTGCTT
GGCAGTAGCGCGTATCAGGAGAAGATTGCTCAGGCCATTTATAAAGGCTTGCGCAGTTATTTCCTCGCGAATCCGTTACA
ATCCGACCCAAAGGTCGAAAGCCGTCCGCTCATTGAAACAGCGGCGGTTGACTCCTCAACACAGCGTTCAGGTATTAGTC
AGCCGGAACCTATTGTGAATAATATAAGCAGTAATGCGCAAAGTGGCCGGTCATCGGCTACTCTGCCTGCAGGTAAGAGC
AAAATCCACAACGTGCAGCGTGGGGAGACGTTATCGGGTATTGCCAGCCAATACGGCGTCAGCATGGCTGTTTTACGACA
AAATAATACGCTGAAAAATGATGTAGTCTGGGTAGGCCAGCGGTTGAAAGTGCCCGCGTCGGGCAGTACGGTAACGGCAG
TCAAGCCTAAAACAGCCGCTGTAGCAAAAAGTAAACCTATTAAGCATCAGGTGAAACGCGGTGATACGCTGTCAGCCATT
GCGGCGAGATACGGTGTATCAATGAGTGAGATTGAGCGGGTGAATAAAATCAAGTCAGGTAATGTTCAGCTTGGGCAAAC
GCTCACTATTCCACAATCGTAA

Upstream 100 bases:

>100_bases
GAAACACGGTTTCGCTACGATTCGCCATTTCACTACGATTTGCCATTTCACTACTGTCCACCGCGTCATTACTGCTCGTC
GTTTCGCCACGGTCACGCGG

Downstream 100 bases:

>100_bases
GCGCAAGGAATTGTTATGCCGATTCAGATTCTGCCTCCACAGCTTGCCAACCAGATTGCCGCCGGTGAGGTGGTAGAGCG
GCCTGCATCGGTAGTCAAAG

Product: putative N-acetylmuramoyl-L-alanine amidase family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 593; Mature: 593

Protein sequence:

>593_residues
MIIGLLTLSLFALPPAFAMKLTDIKVTNGASESKVTLSFDGKPIYAFFSLNSPERVVLDVRQSGNISGLPLEFSGQNLLK
RIRSSTPKDEQSTRLVLELTQKVKTRAVTQQSGNNYTVVLTMTAVASAPVRQAQASLNQTNTPSPNAGRVVPQVTSKDSV
AKNPFNNKPVVVVSSENVTTNTARPIKTVSAANSSRVVVAIDAGHGGQDPGAIGQNGLKEKNVTISIARRLEALLNRDPM
FKPVLTRNGDYFISVMGRSDVARKQGANVLISIHADAAPNRSATGASVWVLSNRRANSEMGNWLEQHEKQSELLGGAGDV
LANTASDPYLSQAVLDLQFGHSQRVGYDVATKVLRELQTVGDIHKRKPEHASLGVLRSPDIPSLLVETGFISNSTEERLL
GSSAYQEKIAQAIYKGLRSYFLANPLQSDPKVESRPLIETAAVDSSTQRSGISQPEPIVNNISSNAQSGRSSATLPAGKS
KIHNVQRGETLSGIASQYGVSMAVLRQNNTLKNDVVWVGQRLKVPASGSTVTAVKPKTAAVAKSKPIKHQVKRGDTLSAI
AARYGVSMSEIERVNKIKSGNVQLGQTLTIPQS

Sequences:

>Translated_593_residues
MIIGLLTLSLFALPPAFAMKLTDIKVTNGASESKVTLSFDGKPIYAFFSLNSPERVVLDVRQSGNISGLPLEFSGQNLLK
RIRSSTPKDEQSTRLVLELTQKVKTRAVTQQSGNNYTVVLTMTAVASAPVRQAQASLNQTNTPSPNAGRVVPQVTSKDSV
AKNPFNNKPVVVVSSENVTTNTARPIKTVSAANSSRVVVAIDAGHGGQDPGAIGQNGLKEKNVTISIARRLEALLNRDPM
FKPVLTRNGDYFISVMGRSDVARKQGANVLISIHADAAPNRSATGASVWVLSNRRANSEMGNWLEQHEKQSELLGGAGDV
LANTASDPYLSQAVLDLQFGHSQRVGYDVATKVLRELQTVGDIHKRKPEHASLGVLRSPDIPSLLVETGFISNSTEERLL
GSSAYQEKIAQAIYKGLRSYFLANPLQSDPKVESRPLIETAAVDSSTQRSGISQPEPIVNNISSNAQSGRSSATLPAGKS
KIHNVQRGETLSGIASQYGVSMAVLRQNNTLKNDVVWVGQRLKVPASGSTVTAVKPKTAAVAKSKPIKHQVKRGDTLSAI
AARYGVSMSEIERVNKIKSGNVQLGQTLTIPQS
>Mature_593_residues
MIIGLLTLSLFALPPAFAMKLTDIKVTNGASESKVTLSFDGKPIYAFFSLNSPERVVLDVRQSGNISGLPLEFSGQNLLK
RIRSSTPKDEQSTRLVLELTQKVKTRAVTQQSGNNYTVVLTMTAVASAPVRQAQASLNQTNTPSPNAGRVVPQVTSKDSV
AKNPFNNKPVVVVSSENVTTNTARPIKTVSAANSSRVVVAIDAGHGGQDPGAIGQNGLKEKNVTISIARRLEALLNRDPM
FKPVLTRNGDYFISVMGRSDVARKQGANVLISIHADAAPNRSATGASVWVLSNRRANSEMGNWLEQHEKQSELLGGAGDV
LANTASDPYLSQAVLDLQFGHSQRVGYDVATKVLRELQTVGDIHKRKPEHASLGVLRSPDIPSLLVETGFISNSTEERLL
GSSAYQEKIAQAIYKGLRSYFLANPLQSDPKVESRPLIETAAVDSSTQRSGISQPEPIVNNISSNAQSGRSSATLPAGKS
KIHNVQRGETLSGIASQYGVSMAVLRQNNTLKNDVVWVGQRLKVPASGSTVTAVKPKTAAVAKSKPIKHQVKRGDTLSAI
AARYGVSMSEIERVNKIKSGNVQLGQTLTIPQS

Specific function: Cell-wall hydrolase involved in septum cleavage during cell division. Can also act as powerful autolysin in the presence of murein synthesis inhibitors [H]

COG id: COG0860

COG function: function code M; N-acetylmuramoyl-L-alanine amidase

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 3 family [H]

Homologues:

Organism=Escherichia coli, GI1790611, Length=430, Percent_Identity=63.2558139534884, Blast_Score=538, Evalue=1e-154,
Organism=Escherichia coli, GI87082163, Length=434, Percent_Identity=30.8755760368664, Blast_Score=182, Evalue=4e-47,
Organism=Escherichia coli, GI1788776, Length=224, Percent_Identity=42.8571428571429, Blast_Score=181, Evalue=1e-46,
Organism=Escherichia coli, GI1786405, Length=111, Percent_Identity=36.9369369369369, Blast_Score=64, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002508 [H]

Pfam domain/function: PF01520 Amidase_3 [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 63477; Mature: 63477

Theoretical pI: Translated: 10.66; Mature: 10.66

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIIGLLTLSLFALPPAFAMKLTDIKVTNGASESKVTLSFDGKPIYAFFSLNSPERVVLDV
CEEHHHHHHHHHCCHHHHEEEEEEEEECCCCCCEEEEEECCCEEEEEEECCCCCEEEEEE
RQSGNISGLPLEFSGQNLLKRIRSSTPKDEQSTRLVLELTQKVKTRAVTQQSGNNYTVVL
ECCCCCCCCEEEECCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEE
TMTAVASAPVRQAQASLNQTNTPSPNAGRVVPQVTSKDSVAKNPFNNKPVVVVSSENVTT
EEEHHHCCHHHHHHHHHCCCCCCCCCCCCEECCCCCCCCCCCCCCCCCCEEEEECCCCCC
NTARPIKTVSAANSSRVVVAIDAGHGGQDPGAIGQNGLKEKNVTISIARRLEALLNRDPM
CCCCCCEEEECCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCEEHHHHHHHHHHHCCCCC
FKPVLTRNGDYFISVMGRSDVARKQGANVLISIHADAAPNRSATGASVWVLSNRRANSEM
CCCCEECCCCEEEEEECCCHHHHHCCCEEEEEEECCCCCCCCCCCCEEEEEECCCCCCHH
GNWLEQHEKQSELLGGAGDVLANTASDPYLSQAVLDLQFGHSQRVGYDVATKVLRELQTV
HHHHHHHHHHHHHCCCCCHHHHCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHH
GDIHKRKPEHASLGVLRSPDIPSLLVETGFISNSTEERLLGSSAYQEKIAQAIYKGLRSY
HHHHHCCCCCCCCCEECCCCCCHHHHHHCCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHH
FLANPLQSDPKVESRPLIETAAVDSSTQRSGISQPEPIVNNISSNAQSGRSSATLPAGKS
HHCCCCCCCCCCCCCCCCHHHHCCCCHHHCCCCCCCHHHHHHHCCCCCCCCCCCCCCCHH
KIHNVQRGETLSGIASQYGVSMAVLRQNNTLKNDVVWVGQRLKVPASGSTVTAVKPKTAA
HHHHCCCCCHHHHHHHHHCCEEEEEECCCCCCCCEEEECCEEECCCCCCEEEEECCCHHH
VAKSKPIKHQVKRGDTLSAIAARYGVSMSEIERVNKIKSGNVQLGQTLTIPQS
HHCCCCHHHHHHCCCHHHHHHHHHCCCHHHHHHHHHHCCCCEEECEEEECCCC
>Mature Secondary Structure
MIIGLLTLSLFALPPAFAMKLTDIKVTNGASESKVTLSFDGKPIYAFFSLNSPERVVLDV
CEEHHHHHHHHHCCHHHHEEEEEEEEECCCCCCEEEEEECCCEEEEEEECCCCCEEEEEE
RQSGNISGLPLEFSGQNLLKRIRSSTPKDEQSTRLVLELTQKVKTRAVTQQSGNNYTVVL
ECCCCCCCCEEEECCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEE
TMTAVASAPVRQAQASLNQTNTPSPNAGRVVPQVTSKDSVAKNPFNNKPVVVVSSENVTT
EEEHHHCCHHHHHHHHHCCCCCCCCCCCCEECCCCCCCCCCCCCCCCCCEEEEECCCCCC
NTARPIKTVSAANSSRVVVAIDAGHGGQDPGAIGQNGLKEKNVTISIARRLEALLNRDPM
CCCCCCEEEECCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCEEHHHHHHHHHHHCCCCC
FKPVLTRNGDYFISVMGRSDVARKQGANVLISIHADAAPNRSATGASVWVLSNRRANSEM
CCCCEECCCCEEEEEECCCHHHHHCCCEEEEEEECCCCCCCCCCCCEEEEEECCCCCCHH
GNWLEQHEKQSELLGGAGDVLANTASDPYLSQAVLDLQFGHSQRVGYDVATKVLRELQTV
HHHHHHHHHHHHHCCCCCHHHHCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHH
GDIHKRKPEHASLGVLRSPDIPSLLVETGFISNSTEERLLGSSAYQEKIAQAIYKGLRSY
HHHHHCCCCCCCCCEECCCCCCHHHHHHCCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHH
FLANPLQSDPKVESRPLIETAAVDSSTQRSGISQPEPIVNNISSNAQSGRSSATLPAGKS
HHCCCCCCCCCCCCCCCCHHHHCCCCHHHCCCCCCCHHHHHHHCCCCCCCCCCCCCCCHH
KIHNVQRGETLSGIASQYGVSMAVLRQNNTLKNDVVWVGQRLKVPASGSTVTAVKPKTAA
HHHHCCCCCHHHHHHHHHCCEEEEEECCCCCCCCEEEECCEEECCCCCCEEEEECCCHHH
VAKSKPIKHQVKRGDTLSAIAARYGVSMSEIERVNKIKSGNVQLGQTLTIPQS
HHCCCCHHHHHHCCCHHHHHHHHHCCCHHHHHHHHHHCCCCEEECEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7511774; 7610040; 9278503; 1594459 [H]