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The map label for this gene is pyrG

Identifier: 161751103

GI number: 161751103

Start: 257613

End: 259208

Strand: Reverse

Name: pyrG

Synonym: ST0237

Alternate gene names: 161751103

Gene position: 259208-257613 (Counterclockwise)

Preceding gene: 15920423

Following gene: 15920417

Centisome position: 9.62

GC content: 34.27

Gene sequence:

>1596_bases
TTGACAAAGTATATTATAATTACTGGAGGAGTATTATCAAGTGTTGGTAAAGGCACGGTAGCTGCTTCTATAGGATTACT
TTTAAAAAATAGAGGATATAAGATAACAATGGTTAAGGTAGATCCATATCTTAATGTTGATGCGGGTACTATGAATCCAT
ATATGCATGGAGAAGTTTTTGTAACAGATGATGGAGCAGAAACAGATCTAGATTTAGGTCATTATGAAAGATTTGTAGGT
ATTAATATGTATAGTTACAATAATATTACTGCTGGTAAGGTTTACTTTGAAGTTATAAAGAATGAGAGGCAAGGAAAATA
TTTGGGTCAAACAGTTCAAATTATACCTCATGTTACGGATCAAATAAAGAGTATGATAAGATATGCTTCTAATATGGCAA
ATGCAGACATTACTATCGTTGAGATAGGCGGAACTGTTGGAGATATAGAAGGTTTACCATTCTTAGAAGCAGTAAGACAA
TTAAAACTAGAAGAGGAAGAAGGAAATGTAGTTTTTGTTCATGTTGCCTTAGTCGAATTCCTTAAAGTAACTGAGGAAAT
AAAAACTAAACCTTTACAACATAGCGTGCAAGAATTAAGAAGAATAGGTATACAGCCAGATATCATAGTTGCTAGATCTG
TAGTTCCACTAGATGAAGAAACAAAAAAGAAGATTGCATTGTTTACGAATGTAAAACCAGAATACATCTTTTCTAACTAT
GATGTCAATATGACTTATGAAGTTCCTCTAATCCTAGAAAAACAAGGCTTAGCAAGTAAGATTTTAAGTAAACTTAACTT
ACCAGATAATCAGCCTAATCTATCTGAATGGATCCGCTTTATAGATAATTTAAAGACAGCTGATAAAGAAGTAAAAATTG
CTCTTGTGGGAAAGTATACAAAGCTTAAAGATAGCTACATTAGTATTAAGGAAGCGATTTACCATGCAGCTGCTAAGCTT
CATATCAAGCCTGTTCTAGTTTGGATAGAATCGACTGACATAGAAAATTCAAAAGAAGCAATTGATAAATTGAAATCTGT
TGACGGGATAATAGTTCTACCCGGATTTGGTTCACGCGGAGTGGAGGGAAAAATTCTTGCAATTAAATATGCGAGAGAAA
ATAATGTACCATTCCTTGGTATCTGTTATGGTATGCAACTAGCAGTAGTAGAATTTGCAAGAAATGTGTTAGGGCTAGAA
AAAGCTCATACTACTGAGGTTGATCCTAATACACCCCATCCAGTAATCACACTATTAGACGAGCAAAAGAAAGTTGTACA
AATTGGTGGTACAATGAGATTAGGATCTCAAAAGGTAATCGTTAAAGAAGGAACATTAGCTTATAGAATTTACGGAAACA
TAGAAGTATATGAGAGGCATAGACATAGATATGAGGTAAACCCAGAATATGTAGATCTTTTACAGAAAGGTGGTCTGGTA
ATATCTGGTGTAAGTGAGAATGGTCTGGTAGAAATGATTGAGCTTAAGAACCATAAATTCTTCCTAGGGTTACAAGGTCA
TCCAGAATATAAAAGTAGGCCTTTAGCTCCTTCACCCGTCTTTATAGGTTTCCTTAGAGCTGCTGCTGGAGTTTAA

Upstream 100 bases:

>100_bases
TTCCAGTAATTCCATCATTCCACCAATAGGTATTTCTACATAAAAGTTAACAAATGATTTTTAAAACTCACTGTGTGATA
TTTTTAGCTGTGATTTCCGA

Downstream 100 bases:

>100_bases
ATTAATAATAATTTTATCATATTTTAATTTTATAATCTTTTTTATACCTTTTATTCCATTTTTATAAGATATTTCAATTA
ATCCAGCATTTTCTAGTTCA

Product: CTP synthetase

Products: NA

Alternate protein names: CTP synthetase; UTP--ammonia ligase

Number of amino acids: Translated: 531; Mature: 530

Protein sequence:

>531_residues
MTKYIIITGGVLSSVGKGTVAASIGLLLKNRGYKITMVKVDPYLNVDAGTMNPYMHGEVFVTDDGAETDLDLGHYERFVG
INMYSYNNITAGKVYFEVIKNERQGKYLGQTVQIIPHVTDQIKSMIRYASNMANADITIVEIGGTVGDIEGLPFLEAVRQ
LKLEEEEGNVVFVHVALVEFLKVTEEIKTKPLQHSVQELRRIGIQPDIIVARSVVPLDEETKKKIALFTNVKPEYIFSNY
DVNMTYEVPLILEKQGLASKILSKLNLPDNQPNLSEWIRFIDNLKTADKEVKIALVGKYTKLKDSYISIKEAIYHAAAKL
HIKPVLVWIESTDIENSKEAIDKLKSVDGIIVLPGFGSRGVEGKILAIKYARENNVPFLGICYGMQLAVVEFARNVLGLE
KAHTTEVDPNTPHPVITLLDEQKKVVQIGGTMRLGSQKVIVKEGTLAYRIYGNIEVYERHRHRYEVNPEYVDLLQKGGLV
ISGVSENGLVEMIELKNHKFFLGLQGHPEYKSRPLAPSPVFIGFLRAAAGV

Sequences:

>Translated_531_residues
MTKYIIITGGVLSSVGKGTVAASIGLLLKNRGYKITMVKVDPYLNVDAGTMNPYMHGEVFVTDDGAETDLDLGHYERFVG
INMYSYNNITAGKVYFEVIKNERQGKYLGQTVQIIPHVTDQIKSMIRYASNMANADITIVEIGGTVGDIEGLPFLEAVRQ
LKLEEEEGNVVFVHVALVEFLKVTEEIKTKPLQHSVQELRRIGIQPDIIVARSVVPLDEETKKKIALFTNVKPEYIFSNY
DVNMTYEVPLILEKQGLASKILSKLNLPDNQPNLSEWIRFIDNLKTADKEVKIALVGKYTKLKDSYISIKEAIYHAAAKL
HIKPVLVWIESTDIENSKEAIDKLKSVDGIIVLPGFGSRGVEGKILAIKYARENNVPFLGICYGMQLAVVEFARNVLGLE
KAHTTEVDPNTPHPVITLLDEQKKVVQIGGTMRLGSQKVIVKEGTLAYRIYGNIEVYERHRHRYEVNPEYVDLLQKGGLV
ISGVSENGLVEMIELKNHKFFLGLQGHPEYKSRPLAPSPVFIGFLRAAAGV
>Mature_530_residues
TKYIIITGGVLSSVGKGTVAASIGLLLKNRGYKITMVKVDPYLNVDAGTMNPYMHGEVFVTDDGAETDLDLGHYERFVGI
NMYSYNNITAGKVYFEVIKNERQGKYLGQTVQIIPHVTDQIKSMIRYASNMANADITIVEIGGTVGDIEGLPFLEAVRQL
KLEEEEGNVVFVHVALVEFLKVTEEIKTKPLQHSVQELRRIGIQPDIIVARSVVPLDEETKKKIALFTNVKPEYIFSNYD
VNMTYEVPLILEKQGLASKILSKLNLPDNQPNLSEWIRFIDNLKTADKEVKIALVGKYTKLKDSYISIKEAIYHAAAKLH
IKPVLVWIESTDIENSKEAIDKLKSVDGIIVLPGFGSRGVEGKILAIKYARENNVPFLGICYGMQLAVVEFARNVLGLEK
AHTTEVDPNTPHPVITLLDEQKKVVQIGGTMRLGSQKVIVKEGTLAYRIYGNIEVYERHRHRYEVNPEYVDLLQKGGLVI
SGVSENGLVEMIELKNHKFFLGLQGHPEYKSRPLAPSPVFIGFLRAAAGV

Specific function: Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen

COG id: COG0504

COG function: function code F; CTP synthase (UTP-ammonia lyase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain

Homologues:

Organism=Homo sapiens, GI28559085, Length=549, Percent_Identity=46.2659380692168, Blast_Score=507, Evalue=1e-144,
Organism=Homo sapiens, GI28559083, Length=549, Percent_Identity=46.2659380692168, Blast_Score=507, Evalue=1e-144,
Organism=Homo sapiens, GI221316689, Length=549, Percent_Identity=46.2659380692168, Blast_Score=507, Evalue=1e-144,
Organism=Homo sapiens, GI148491070, Length=549, Percent_Identity=46.6302367941712, Blast_Score=501, Evalue=1e-142,
Organism=Escherichia coli, GI1789142, Length=541, Percent_Identity=46.2107208872458, Blast_Score=481, Evalue=1e-137,
Organism=Caenorhabditis elegans, GI25148299, Length=604, Percent_Identity=40.7284768211921, Blast_Score=447, Evalue=1e-126,
Organism=Saccharomyces cerevisiae, GI6322563, Length=559, Percent_Identity=44.3649373881932, Blast_Score=477, Evalue=1e-135,
Organism=Saccharomyces cerevisiae, GI6319432, Length=562, Percent_Identity=43.0604982206406, Blast_Score=473, Evalue=1e-134,
Organism=Drosophila melanogaster, GI24664469, Length=547, Percent_Identity=47.1663619744059, Blast_Score=501, Evalue=1e-142,
Organism=Drosophila melanogaster, GI21357815, Length=492, Percent_Identity=45.7317073170732, Blast_Score=425, Evalue=1e-119,

Paralogues:

None

Copy number: 480 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): PYRG_SULTO (Q976E9)

Other databases:

- EMBL:   BA000023
- RefSeq:   NP_376089.2
- ProteinModelPortal:   Q976E9
- SMR:   Q976E9
- GeneID:   1458128
- GenomeReviews:   BA000023_GR
- KEGG:   sto:ST0237
- NMPDR:   fig|273063.1.peg.257
- HOGENOM:   HBG597806
- ProtClustDB:   PRK05380
- BioCyc:   STOK273063:ST0237-MONOMER
- BRENDA:   6.3.4.2
- HAMAP:   MF_01227
- InterPro:   IPR004468
- InterPro:   IPR017456
- InterPro:   IPR017926
- InterPro:   IPR000991
- TIGRFAMs:   TIGR00337

Pfam domain/function: PF06418 CTP_synth_N; PF00117 GATase

EC number: =6.3.4.2

Molecular weight: Translated: 59239; Mature: 59107

Theoretical pI: Translated: 7.22; Mature: 7.22

Prosite motif: PS51273 GATASE_TYPE_1; PS00442 GATASE_TYPE_I

Important sites: ACT_SITE 382-382 ACT_SITE 507-507 ACT_SITE 509-509

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKYIIITGGVLSSVGKGTVAASIGLLLKNRGYKITMVKVDPYLNVDAGTMNPYMHGEVF
CCEEEEEECHHHHHCCCCHHHHHHHHHEECCCCEEEEEEECCEEECCCCCCCCCCCCEEE
VTDDGAETDLDLGHYERFVGINMYSYNNITAGKVYFEVIKNERQGKYLGQTVQIIPHVTD
EECCCCCCCCCCHHHHHEEEEEEEECCCEEHHHHHEEEHHCCCCCCHHCCHHEEHHHHHH
QIKSMIRYASNMANADITIVEIGGTVGDIEGLPFLEAVRQLKLEEEEGNVVFVHVALVEF
HHHHHHHHHHHCCCCCEEEEEECCCEECCCCCCHHHHHHHHCCCCCCCCEEEEEHHHHHH
LKVTEEIKTKPLQHSVQELRRIGIQPDIIVARSVVPLDEETKKKIALFTNVKPEYIFSNY
HHHHHHHHCCHHHHHHHHHHHCCCCCCEEEEEEECCCCCHHCCEEEEEECCCCCEEEECC
DVNMTYEVPLILEKQGLASKILSKLNLPDNQPNLSEWIRFIDNLKTADKEVKIALVGKYT
CEEEEEEEEEEEECCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCEEEEEEEECCH
KLKDSYISIKEAIYHAAAKLHIKPVLVWIESTDIENSKEAIDKLKSVDGIIVLPGFGSRG
HHHHHHHHHHHHHHHHHHEEEEEEEEEEEECCCCCCHHHHHHHHHCCCCEEEECCCCCCC
VEGKILAIKYARENNVPFLGICYGMQLAVVEFARNVLGLEKAHTTEVDPNTPHPVITLLD
CCCEEEEEEEECCCCCCEEEEEHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEC
EQKKVVQIGGTMRLGSQKVIVKEGTLAYRIYGNIEVYERHRHRYEVNPEYVDLLQKGGLV
CCCEEEEECCEEEECCEEEEEECCCEEEEEECCEEEEECCCCCCCCCHHHHHHHHHCCEE
ISGVSENGLVEMIELKNHKFFLGLQGHPEYKSRPLAPSPVFIGFLRAAAGV
EEECCCCCCEEEEEECCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHCCC
>Mature Secondary Structure 
TKYIIITGGVLSSVGKGTVAASIGLLLKNRGYKITMVKVDPYLNVDAGTMNPYMHGEVF
CEEEEEECHHHHHCCCCHHHHHHHHHEECCCCEEEEEEECCEEECCCCCCCCCCCCEEE
VTDDGAETDLDLGHYERFVGINMYSYNNITAGKVYFEVIKNERQGKYLGQTVQIIPHVTD
EECCCCCCCCCCHHHHHEEEEEEEECCCEEHHHHHEEEHHCCCCCCHHCCHHEEHHHHHH
QIKSMIRYASNMANADITIVEIGGTVGDIEGLPFLEAVRQLKLEEEEGNVVFVHVALVEF
HHHHHHHHHHHCCCCCEEEEEECCCEECCCCCCHHHHHHHHCCCCCCCCEEEEEHHHHHH
LKVTEEIKTKPLQHSVQELRRIGIQPDIIVARSVVPLDEETKKKIALFTNVKPEYIFSNY
HHHHHHHHCCHHHHHHHHHHHCCCCCCEEEEEEECCCCCHHCCEEEEEECCCCCEEEECC
DVNMTYEVPLILEKQGLASKILSKLNLPDNQPNLSEWIRFIDNLKTADKEVKIALVGKYT
CEEEEEEEEEEEECCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCEEEEEEEECCH
KLKDSYISIKEAIYHAAAKLHIKPVLVWIESTDIENSKEAIDKLKSVDGIIVLPGFGSRG
HHHHHHHHHHHHHHHHHHEEEEEEEEEEEECCCCCCHHHHHHHHHCCCCEEEECCCCCCC
VEGKILAIKYARENNVPFLGICYGMQLAVVEFARNVLGLEKAHTTEVDPNTPHPVITLLD
CCCEEEEEEEECCCCCCEEEEEHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEC
EQKKVVQIGGTMRLGSQKVIVKEGTLAYRIYGNIEVYERHRHRYEVNPEYVDLLQKGGLV
CCCEEEEECCEEEECCEEEEEECCCEEEEEECCEEEEECCCCCCCCCHHHHHHHHHCCEE
ISGVSENGLVEMIELKNHKFFLGLQGHPEYKSRPLAPSPVFIGFLRAAAGV
EEECCCCCCEEEEEECCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11572479