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The map label for this gene is dphB

Identifier: 15921541

GI number: 15921541

Start: 1275121

End: 1275885

Strand: Direct

Name: dphB

Synonym: ST1278

Alternate gene names: 15921541

Gene position: 1275121-1275885 (Clockwise)

Preceding gene: 15921538

Following gene: 15921542

Centisome position: 47.32

GC content: 29.02

Gene sequence:

>765_bases
ATGCCAACGCTTAAGCTTATAGGGTTGGGCCTCTCTGCTAAATTTGTAACTAGAGAAGCAATTGATGAGATAAGTAAATG
TAATGTTGTGCTATTCGAGAGTTATACTTCTCTATCTTGTGATATAAATTTAGATTTTATTAAATTTTTAAATAAAAATG
TTATTATCGTAGATAGAAAATTTATTGAAAATAATATTAAAGAAATTATTAAACTGTTAAAAGAGAAAGAAGATGTTTGT
ATAGTAACTATAGGGGATCCTATGATTGCAACAACACATGTGAGTCTTATTGTTGAGGTGAAAGACAAGGGCTATAATTT
TAAAGTAATTCCGGGGATATCAGTTCATTGCTATATAATTTCAAAATCTATGTTATCTTCCTATAAATTTGGTAAATCTG
TAACTATAACTTACCCTTATAATAATAAAATTGATACGACTCCCTATGATGTAATTTATGATAATTTTATACGGGGACTC
CACACAATACTATATCTTGACTTAAAAGAAGATAAAATTATGACAGCTAAAGAAGCCGTAGAACTTCTAATAGAAATGGA
GAAAATAAAGAAGCAAGGATTAGTTAGTGATGATAGAATCATCATAGTAGGTCAGAGATTGGGATGCGATGATGAGGAAG
TAGTTGCATTACGATTAAAGGAAGTATTTAATTATAAATTTAAAGAACCACCACATATTATTGTATTTCCTACAGATAAG
TTACACTTCATGGAGGTAGAGGCTTTAAAATGTCTAATGAAATAA

Upstream 100 bases:

>100_bases
ATATAACGGGTAAATCTTCATCAATGTAAATATCATCAACTAAACTTATCATCTTGTTCTTATAATCTAATTTAATACTA
ATAAATACCAATTTAAAAAC

Downstream 100 bases:

>100_bases
GAAATAGAGTAGAAAAATATATAAAAGGGATGGAAGAAAGATTACAAAAAATACCTAGTCATATCTATGAAAAATATCGT
AAAGTACTAGATCTTGCTAA

Product: diphthine synthase

Products: NA

Alternate protein names: Diphthamide biosynthesis methyltransferase

Number of amino acids: Translated: 254; Mature: 253

Protein sequence:

>254_residues
MPTLKLIGLGLSAKFVTREAIDEISKCNVVLFESYTSLSCDINLDFIKFLNKNVIIVDRKFIENNIKEIIKLLKEKEDVC
IVTIGDPMIATTHVSLIVEVKDKGYNFKVIPGISVHCYIISKSMLSSYKFGKSVTITYPYNNKIDTTPYDVIYDNFIRGL
HTILYLDLKEDKIMTAKEAVELLIEMEKIKKQGLVSDDRIIIVGQRLGCDDEEVVALRLKEVFNYKFKEPPHIIVFPTDK
LHFMEVEALKCLMK

Sequences:

>Translated_254_residues
MPTLKLIGLGLSAKFVTREAIDEISKCNVVLFESYTSLSCDINLDFIKFLNKNVIIVDRKFIENNIKEIIKLLKEKEDVC
IVTIGDPMIATTHVSLIVEVKDKGYNFKVIPGISVHCYIISKSMLSSYKFGKSVTITYPYNNKIDTTPYDVIYDNFIRGL
HTILYLDLKEDKIMTAKEAVELLIEMEKIKKQGLVSDDRIIIVGQRLGCDDEEVVALRLKEVFNYKFKEPPHIIVFPTDK
LHFMEVEALKCLMK
>Mature_253_residues
PTLKLIGLGLSAKFVTREAIDEISKCNVVLFESYTSLSCDINLDFIKFLNKNVIIVDRKFIENNIKEIIKLLKEKEDVCI
VTIGDPMIATTHVSLIVEVKDKGYNFKVIPGISVHCYIISKSMLSSYKFGKSVTITYPYNNKIDTTPYDVIYDNFIRGLH
TILYLDLKEDKIMTAKEAVELLIEMEKIKKQGLVSDDRIIIVGQRLGCDDEEVVALRLKEVFNYKFKEPPHIIVFPTDKL
HFMEVEALKCLMK

Specific function: Required for the methylation step in diphthamide biosynthesis

COG id: COG1798

COG function: function code J; Diphthamide biosynthesis methyltransferase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the diphthine synthase family

Homologues:

Organism=Homo sapiens, GI117190324, Length=267, Percent_Identity=26.5917602996255, Blast_Score=105, Evalue=3e-23,
Organism=Homo sapiens, GI117190326, Length=268, Percent_Identity=26.865671641791, Blast_Score=103, Evalue=2e-22,
Organism=Homo sapiens, GI117190262, Length=268, Percent_Identity=26.865671641791, Blast_Score=103, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI17531369, Length=271, Percent_Identity=29.520295202952, Blast_Score=101, Evalue=4e-22,
Organism=Saccharomyces cerevisiae, GI6323201, Length=258, Percent_Identity=29.4573643410853, Blast_Score=108, Evalue=1e-24,
Organism=Drosophila melanogaster, GI28572120, Length=268, Percent_Identity=27.9850746268657, Blast_Score=104, Evalue=5e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DPHB_SULTO (Q971V1)

Other databases:

- EMBL:   BA000023
- RefSeq:   NP_377210.1
- ProteinModelPortal:   Q971V1
- SMR:   Q971V1
- GeneID:   1459277
- GenomeReviews:   BA000023_GR
- KEGG:   sto:ST1278
- NMPDR:   fig|273063.1.peg.1378
- HOGENOM:   HBG460511
- OMA:   YAEFYTA
- ProtClustDB:   PRK04160
- BioCyc:   STOK273063:ST1278-MONOMER
- BRENDA:   2.1.1.98
- HAMAP:   MF_01084
- InterPro:   IPR000878
- InterPro:   IPR014777
- InterPro:   IPR014776
- InterPro:   IPR004551
- Gene3D:   G3DSA:3.40.1010.10
- Gene3D:   G3DSA:3.30.950.10
- PANTHER:   PTHR10882
- PIRSF:   PIRSF036432
- TIGRFAMs:   TIGR00522

Pfam domain/function: PF00590 TP_methylase; SSF53790 Cor/por_Metransf

EC number: =2.1.1.98

Molecular weight: Translated: 29137; Mature: 29006

Theoretical pI: Translated: 7.01; Mature: 7.01

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPTLKLIGLGLSAKFVTREAIDEISKCNVVLFESYTSLSCDINLDFIKFLNKNVIIVDRK
CCCEEEEEECCCHHHHHHHHHHHHHHCCEEEEECCCEEEEEECHHHHHHHCCCEEEEEHH
FIENNIKEIIKLLKEKEDVCIVTIGDPMIATTHVSLIVEVKDKGYNFKVIPGISVHCYII
HHHHHHHHHHHHHHCCCCEEEEEECCCEEEEEEEEEEEEEECCCCCEEEECCCEEEEEEE
SKSMLSSYKFGKSVTITYPYNNKIDTTPYDVIYDNFIRGLHTILYLDLKEDKIMTAKEAV
EHHHHHHHCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHEEEEEEECCCCCHHHHHHH
ELLIEMEKIKKQGLVSDDRIIIVGQRLGCDDEEVVALRLKEVFNYKFKEPPHIIVFPTDK
HHHHHHHHHHHCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHCCCCCCCCEEEEEECCC
LHFMEVEALKCLMK
EEEEEHHHHHHHCC
>Mature Secondary Structure 
PTLKLIGLGLSAKFVTREAIDEISKCNVVLFESYTSLSCDINLDFIKFLNKNVIIVDRK
CCEEEEEECCCHHHHHHHHHHHHHHCCEEEEECCCEEEEEECHHHHHHHCCCEEEEEHH
FIENNIKEIIKLLKEKEDVCIVTIGDPMIATTHVSLIVEVKDKGYNFKVIPGISVHCYII
HHHHHHHHHHHHHHCCCCEEEEEECCCEEEEEEEEEEEEEECCCCCEEEECCCEEEEEEE
SKSMLSSYKFGKSVTITYPYNNKIDTTPYDVIYDNFIRGLHTILYLDLKEDKIMTAKEAV
EHHHHHHHCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHEEEEEEECCCCCHHHHHHH
ELLIEMEKIKKQGLVSDDRIIIVGQRLGCDDEEVVALRLKEVFNYKFKEPPHIIVFPTDK
HHHHHHHHHHHCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHCCCCCCCCEEEEEECCC
LHFMEVEALKCLMK
EEEEEHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11572479