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The map label for this gene is mcrB [H]

Identifier: 15921409

GI number: 15921409

Start: 1149957

End: 1151612

Strand: Direct

Name: mcrB [H]

Synonym: ST1151

Alternate gene names: 15921409

Gene position: 1149957-1151612 (Clockwise)

Preceding gene: 15921408

Following gene: 15921410

Centisome position: 42.67

GC content: 35.87

Gene sequence:

>1656_bases
GTGGTAATTTTTTCCGTGTATGGGGACTGGCTTACAGTAAGGGACGCGTTTAAGAACTTGTTAGGCTTTTCTGAAGAAGC
AGTTAGCACATTCCTAGATTATGTGCAAAATGGAAAATTTATACCTTGTCTTTTCATAGGTTCCAAAGAGCACTGGATAG
AGTCTATAAGATATTCTTTTGAGGGAGAAATAGGCTATACACTTTGGGGATCTGCAGATAAGTCATCAATTTCTGGTATT
TTAAGCAGATATCAGATGGTATATTTAAATACTGATAAGAGCAGATATATTCCAGATAATAACAAAATTCTTATTGCAAT
GCTATATATGACAGGTTCTGGAATAATAGGATTCGGAGTAGTAACTGACGTGGAAATGGATGCTATTAGAAACTTTAAGG
GATGGAAAGAAGTTTCTGGTAAATTCTGGGTACTCAGATTTAGAATAAAAGTCTTTTGGCTTCATGAGAGTATAAGGAAA
AATAAGGACATAAATGCATGGACTGGAGAAAGCTATGAATTAAAAGGAACAAATTCGCAGGCAAATATGTGCTACAGCCA
AGAGAATAACGATGCTATAAATAGCTTCAAGAGTTTTATTTTATCAAAGAAGGACGAGATCAAACCCACTCTTGACCTTT
ATCTAAGCTTGCTAGGCAGTAAACCTAAAGAAGTTGAGGAAAATAAAAAAGAAGTAATATGTAAGGGAGCAGGACAAATA
AATGTGAATGACCTATATCTTCCCCCAAACGCTCTGGATTTAATTTTAAGTTCAGTAAAGAGAACTAACGTGCTTTTAGT
AGGCCCTCCAGGTACTGGTAAGACAAGCTTGGCTGTAAGAGTAGCTAAGTCACTTACTGGGAATGACGAGTGTTACGAAG
TCGCCACTGCGAATTCGTTATGGTTTAGGAGGAACTTAGTAGGTGGAGAAAGCATTAGAGGAGGGAGTGTAATATGGAAG
AGCGGTCTCTTTATCCAAGCTTATGTAAATGCATATAAAGTGAAGGAGGGAAACTATTTCCTTATTATAGATGAAATAAA
TAGGGCTGATGTGGATAAAGCCTTCGGAGAGTTAATAACAATATTCTCAAGCCCTTCTCCAGATGAATGGTCAATTCCCA
GATCACTTATAGACGAAATAAAAAGCTATGGAGATAATGTAGATAGTACTGCAAAGGAATTTCTCAGAATATATGAAGAT
CTGAAAAAGGAGAATAGGGAAAACGAGCCATTGAGAAAAATGCGAATTATCGCTACAATGAACCTGGTAGATGCTAGAAA
CCTCTTTTATATAGGAGATGCACTAGCTAGAAGATTTGTAATTATTAAATTTGATTATCCAGAGGGGACAGAAGATCTAG
ATAAACTCATTGACAGGTATAACCTCAGAGATGATGAAAAGAATGAGATAAGAAACTTAATAGGATGTCTAAGAGCCAAA
CTGAAAAAAGACGAAAAGGATGCGTTAGTGAAATTTAACGTATCTCCAGCAAGTATAAAGACAGCACTTGACATTTACTC
TTCTTTAGAGAACAAGGGAATAGATAAGTTCATAGAAGTGCTCGAGTCAACCCTTGGTACTTTAGATCAAAAGAATATTA
GTAGAGTTAAAAGTAAAATAGAAGAATGCAGAAAAGGCGAAAATCGTAAAATCTAA

Upstream 100 bases:

>100_bases
CATGATGATAGCGGATCCTTTAGTTAAAGATGATAAGGAGAACATTGATAGGATAGATAAAATACTCGGTAAGTACTTAT
AAAAATATATATACTGAAAA

Downstream 100 bases:

>100_bases
CACTTTAATTCTTTTGAAGATTAAACAAATTAGACACAAAGATATTAATCTTTATTTCTTTTCTCACCTTCTCTTTTTAT
TAATCTTTAAGTACTTCTCT

Product: 5-methylcytosine-specific restriction enzyme

Products: NA

Alternate protein names: EcoKMcrBC [H]

Number of amino acids: Translated: 551; Mature: 551

Protein sequence:

>551_residues
MVIFSVYGDWLTVRDAFKNLLGFSEEAVSTFLDYVQNGKFIPCLFIGSKEHWIESIRYSFEGEIGYTLWGSADKSSISGI
LSRYQMVYLNTDKSRYIPDNNKILIAMLYMTGSGIIGFGVVTDVEMDAIRNFKGWKEVSGKFWVLRFRIKVFWLHESIRK
NKDINAWTGESYELKGTNSQANMCYSQENNDAINSFKSFILSKKDEIKPTLDLYLSLLGSKPKEVEENKKEVICKGAGQI
NVNDLYLPPNALDLILSSVKRTNVLLVGPPGTGKTSLAVRVAKSLTGNDECYEVATANSLWFRRNLVGGESIRGGSVIWK
SGLFIQAYVNAYKVKEGNYFLIIDEINRADVDKAFGELITIFSSPSPDEWSIPRSLIDEIKSYGDNVDSTAKEFLRIYED
LKKENRENEPLRKMRIIATMNLVDARNLFYIGDALARRFVIIKFDYPEGTEDLDKLIDRYNLRDDEKNEIRNLIGCLRAK
LKKDEKDALVKFNVSPASIKTALDIYSSLENKGIDKFIEVLESTLGTLDQKNISRVKSKIEECRKGENRKI

Sequences:

>Translated_551_residues
MVIFSVYGDWLTVRDAFKNLLGFSEEAVSTFLDYVQNGKFIPCLFIGSKEHWIESIRYSFEGEIGYTLWGSADKSSISGI
LSRYQMVYLNTDKSRYIPDNNKILIAMLYMTGSGIIGFGVVTDVEMDAIRNFKGWKEVSGKFWVLRFRIKVFWLHESIRK
NKDINAWTGESYELKGTNSQANMCYSQENNDAINSFKSFILSKKDEIKPTLDLYLSLLGSKPKEVEENKKEVICKGAGQI
NVNDLYLPPNALDLILSSVKRTNVLLVGPPGTGKTSLAVRVAKSLTGNDECYEVATANSLWFRRNLVGGESIRGGSVIWK
SGLFIQAYVNAYKVKEGNYFLIIDEINRADVDKAFGELITIFSSPSPDEWSIPRSLIDEIKSYGDNVDSTAKEFLRIYED
LKKENRENEPLRKMRIIATMNLVDARNLFYIGDALARRFVIIKFDYPEGTEDLDKLIDRYNLRDDEKNEIRNLIGCLRAK
LKKDEKDALVKFNVSPASIKTALDIYSSLENKGIDKFIEVLESTLGTLDQKNISRVKSKIEECRKGENRKI
>Mature_551_residues
MVIFSVYGDWLTVRDAFKNLLGFSEEAVSTFLDYVQNGKFIPCLFIGSKEHWIESIRYSFEGEIGYTLWGSADKSSISGI
LSRYQMVYLNTDKSRYIPDNNKILIAMLYMTGSGIIGFGVVTDVEMDAIRNFKGWKEVSGKFWVLRFRIKVFWLHESIRK
NKDINAWTGESYELKGTNSQANMCYSQENNDAINSFKSFILSKKDEIKPTLDLYLSLLGSKPKEVEENKKEVICKGAGQI
NVNDLYLPPNALDLILSSVKRTNVLLVGPPGTGKTSLAVRVAKSLTGNDECYEVATANSLWFRRNLVGGESIRGGSVIWK
SGLFIQAYVNAYKVKEGNYFLIIDEINRADVDKAFGELITIFSSPSPDEWSIPRSLIDEIKSYGDNVDSTAKEFLRIYED
LKKENRENEPLRKMRIIATMNLVDARNLFYIGDALARRFVIIKFDYPEGTEDLDKLIDRYNLRDDEKNEIRNLIGCLRAK
LKKDEKDALVKFNVSPASIKTALDIYSSLENKGIDKFIEVLESTLGTLDQKNISRVKSKIEECRKGENRKI

Specific function: Recognizes N4- and C5-methylcytosine (and 5-hydroxy- methylcytosines) produced by a broad range of DNA methylases and appears to act against 5-methylcytosine preceded by a purine residue. Binds to DNA containing methylated cytosines; also binds to GTP. Is

COG id: COG1401

COG function: function code V; GTPase subunit of restriction endonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI87082429, Length=218, Percent_Identity=31.651376146789, Blast_Score=79, Evalue=6e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011704
- InterPro:   IPR021961
- InterPro:   IPR004491 [H]

Pfam domain/function: PF07728 AAA_5; PF12102 DUF3578 [H]

EC number: 3.1.21.- [C]

Molecular weight: Translated: 62717; Mature: 62717

Theoretical pI: Translated: 8.05; Mature: 8.05

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVIFSVYGDWLTVRDAFKNLLGFSEEAVSTFLDYVQNGKFIPCLFIGSKEHWIESIRYSF
CEEEEEECCHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCEEEEEEECCHHHHHHHHHHHC
EGEIGYTLWGSADKSSISGILSRYQMVYLNTDKSRYIPDNNKILIAMLYMTGSGIIGFGV
CCCCCEEEECCCCHHHHHHHHHCEEEEEEECCCCCCCCCCCCEEEEEEEECCCCEEEEEE
VTDVEMDAIRNFKGWKEVSGKFWVLRFRIKVFWLHESIRKNKDINAWTGESYELKGTNSQ
EECCCHHHHHCCCCHHHCCCCEEEEEEEEEEEEEEHHHHCCCCCCCCCCCCEEECCCCCC
ANMCYSQENNDAINSFKSFILSKKDEIKPTLDLYLSLLGSKPKEVEENKKEVICKGAGQI
CCEEECCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCHHHHCCCHHEEEECCCEE
NVNDLYLPPNALDLILSSVKRTNVLLVGPPGTGKTSLAVRVAKSLTGNDECYEVATANSL
CCCEEEECCHHHHHHHHHHHHCCEEEECCCCCCCCHHHHHHHHHCCCCCHHEEEECCCCH
WFRRNLVGGESIRGGSVIWKSGLFIQAYVNAYKVKEGNYFLIIDEINRADVDKAFGELIT
HHHHCCCCCCCCCCCCEEEECCCEEEEEEEEEEEECCCEEEEEECCCCCHHHHHHHHHEE
IFSSPSPDEWSIPRSLIDEIKSYGDNVDSTAKEFLRIYEDLKKENRENEPLRKMRIIATM
EECCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
NLVDARNLFYIGDALARRFVIIKFDYPEGTEDLDKLIDRYNLRDDEKNEIRNLIGCLRAK
HHHCCCCEEEECHHHHCEEEEEEEECCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHH
LKKDEKDALVKFNVSPASIKTALDIYSSLENKGIDKFIEVLESTLGTLDQKNISRVKSKI
HCCCCCCCEEEECCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHHH
EECRKGENRKI
HHHHCCCCCCC
>Mature Secondary Structure
MVIFSVYGDWLTVRDAFKNLLGFSEEAVSTFLDYVQNGKFIPCLFIGSKEHWIESIRYSF
CEEEEEECCHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCEEEEEEECCHHHHHHHHHHHC
EGEIGYTLWGSADKSSISGILSRYQMVYLNTDKSRYIPDNNKILIAMLYMTGSGIIGFGV
CCCCCEEEECCCCHHHHHHHHHCEEEEEEECCCCCCCCCCCCEEEEEEEECCCCEEEEEE
VTDVEMDAIRNFKGWKEVSGKFWVLRFRIKVFWLHESIRKNKDINAWTGESYELKGTNSQ
EECCCHHHHHCCCCHHHCCCCEEEEEEEEEEEEEEHHHHCCCCCCCCCCCCEEECCCCCC
ANMCYSQENNDAINSFKSFILSKKDEIKPTLDLYLSLLGSKPKEVEENKKEVICKGAGQI
CCEEECCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCHHHHCCCHHEEEECCCEE
NVNDLYLPPNALDLILSSVKRTNVLLVGPPGTGKTSLAVRVAKSLTGNDECYEVATANSL
CCCEEEECCHHHHHHHHHHHHCCEEEECCCCCCCCHHHHHHHHHCCCCCHHEEEECCCCH
WFRRNLVGGESIRGGSVIWKSGLFIQAYVNAYKVKEGNYFLIIDEINRADVDKAFGELIT
HHHHCCCCCCCCCCCCEEEECCCEEEEEEEEEEEECCCEEEEEECCCCCHHHHHHHHHEE
IFSSPSPDEWSIPRSLIDEIKSYGDNVDSTAKEFLRIYEDLKKENRENEPLRKMRIIATM
EECCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
NLVDARNLFYIGDALARRFVIIKFDYPEGTEDLDKLIDRYNLRDDEKNEIRNLIGCLRAK
HHHCCCCEEEECHHHHCEEEEEEEECCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHH
LKKDEKDALVKFNVSPASIKTALDIYSSLENKGIDKFIEVLESTLGTLDQKNISRVKSKI
HCCCCCCCEEEECCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHHH
EECRKGENRKI
HHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on ester bonds; Endodeoxyribonucleases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2203735; 2649480; 7610040; 9278503; 1312983; 7781618 [H]