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The map label for this gene is purB [H]

Identifier: 15920480

GI number: 15920480

Start: 306812

End: 308164

Strand: Reverse

Name: purB [H]

Synonym: ST0288

Alternate gene names: 15920480

Gene position: 308164-306812 (Counterclockwise)

Preceding gene: 15920486

Following gene: 15920479

Centisome position: 11.44

GC content: 33.7

Gene sequence:

>1353_bases
GTGGAAAATTCAGTTTGTGTATTAGACTGGAGATACGGAAGCAAAGAGATGAGAGAATTATTCCAAAGAGCTACAATTCT
TAAAAGAATGGCTACAGTAGAAGTAGCGTTACTTTATGCTTTATCAAAGATAGGTTTAGTAAGAGAAGAGGATGTACTGG
TTGTGCAAAAAAACATAGACAAAATAAATATAAGCAGAGTAGAAGAATTAGAGAAGAAATTAGGACATGATGTTATGGCA
TTAACTGTGCATTTAGCTGAATTATCTGGCGAATCTGGTAAATTTATTCATTTTGGTGCTACAAGTTATGACATAGTAGA
TACAGCTAATGTATTAATTTTTAGGGATGCCATAAATATAATAAAGAAAAAATTAAAAAATATAATAATAATATTAATGG
AATATGCTGAAAAATACCAAGAACTTGTAATGGTAGGTAGAACACATGGGCAACACGCGTTACCAATAACGCTGGGTTTT
AAATTTGCAAATTACGTTTATGAGTTTACTCGTTCGTTAGAGAGGCTTAACGATACAAGCAAAAGATTACTTAAAATAAA
AATGGCTGGGGCAGTAGGCACAATGGCAGGTTGGAAAGATAAAGGTTTAGAGATAGAAAAATATGTTTCAGAATACTTAG
GATTACCTCCTCATGAAATCTCTACTCAGATAGCTCCTCGTGACGGATATACTGAGTTAGTCTCAGATCTTGCAATTTTA
GCGTCTCAGCTTGACAGATTTGCCTTAGAAATAAGGGAGCTAATGAGACCTGAAATTTTAGAGTTAGCTGAAGGTTCTGC
AGAATCAAGAGTAGGAAGTAGTACTATGCCTCATAAGGAGAATCCAGTAACTGCGGAAAAAATCAGTGGATTAGCCAAGG
TATTGAGGGGCTTTGTAATATCTGAGCTAGAAAATATACCTTTATGGCATGAAAGAGATCTAACTAACAGCTCATCTGAA
AGGATAATTCTTTCTCACTCTTTTCTTATAATGGATGAGATGTTAGAGAGTATGGAAGCTCTTTTAAGAAATCTAAGAGT
TTACCCAGAAAATATGAGAAAGAACCTAGAACTCACTAAAGGTCTTATTATGGCTGAAAGCTTAATGATTAATCTTACAT
TAGCAAATGTTCCAAGGCATATAGCTCATGAACTTGTTATGAAAGTATCTAGAGAAGCAGAAAGAGAAGGGAAATCATTG
CTTGAAGCTGCTTTAGACAATGAGGAAATAGTTAAAATACTAGGTAAGGAGAAAATAATAGAAGCATTAAATCCGTATAG
CTATTTAGGAGAATACAAGAAATTAATAAGTAGAGCATTATCATATGCAAAGAATGTTATTGAACAGACGTAA

Upstream 100 bases:

>100_bases
TTTTTGCTCCATGTAATATTTGTAATGATGAATGACTTCCTATCGTAAGTATGTACATTAATAAAAAATCACATATACTA
CTTAATAAATAATTTCTCCC

Downstream 100 bases:

>100_bases
AAATTTTATTTTGATACTCTAAATTATAATATGCCGCCGTAGCTCAGTCTGGTAGAGCGCCGGCCTCGTAAGGTTCCTAA
GAAGGATCCAAGAAAGCCGG

Product: adenylosuccinate lyase

Products: NA

Alternate protein names: ASL; Adenylosuccinase; ASase [H]

Number of amino acids: Translated: 450; Mature: 450

Protein sequence:

>450_residues
MENSVCVLDWRYGSKEMRELFQRATILKRMATVEVALLYALSKIGLVREEDVLVVQKNIDKINISRVEELEKKLGHDVMA
LTVHLAELSGESGKFIHFGATSYDIVDTANVLIFRDAINIIKKKLKNIIIILMEYAEKYQELVMVGRTHGQHALPITLGF
KFANYVYEFTRSLERLNDTSKRLLKIKMAGAVGTMAGWKDKGLEIEKYVSEYLGLPPHEISTQIAPRDGYTELVSDLAIL
ASQLDRFALEIRELMRPEILELAEGSAESRVGSSTMPHKENPVTAEKISGLAKVLRGFVISELENIPLWHERDLTNSSSE
RIILSHSFLIMDEMLESMEALLRNLRVYPENMRKNLELTKGLIMAESLMINLTLANVPRHIAHELVMKVSREAEREGKSL
LEAALDNEEIVKILGKEKIIEALNPYSYLGEYKKLISRALSYAKNVIEQT

Sequences:

>Translated_450_residues
MENSVCVLDWRYGSKEMRELFQRATILKRMATVEVALLYALSKIGLVREEDVLVVQKNIDKINISRVEELEKKLGHDVMA
LTVHLAELSGESGKFIHFGATSYDIVDTANVLIFRDAINIIKKKLKNIIIILMEYAEKYQELVMVGRTHGQHALPITLGF
KFANYVYEFTRSLERLNDTSKRLLKIKMAGAVGTMAGWKDKGLEIEKYVSEYLGLPPHEISTQIAPRDGYTELVSDLAIL
ASQLDRFALEIRELMRPEILELAEGSAESRVGSSTMPHKENPVTAEKISGLAKVLRGFVISELENIPLWHERDLTNSSSE
RIILSHSFLIMDEMLESMEALLRNLRVYPENMRKNLELTKGLIMAESLMINLTLANVPRHIAHELVMKVSREAEREGKSL
LEAALDNEEIVKILGKEKIIEALNPYSYLGEYKKLISRALSYAKNVIEQT
>Mature_450_residues
MENSVCVLDWRYGSKEMRELFQRATILKRMATVEVALLYALSKIGLVREEDVLVVQKNIDKINISRVEELEKKLGHDVMA
LTVHLAELSGESGKFIHFGATSYDIVDTANVLIFRDAINIIKKKLKNIIIILMEYAEKYQELVMVGRTHGQHALPITLGF
KFANYVYEFTRSLERLNDTSKRLLKIKMAGAVGTMAGWKDKGLEIEKYVSEYLGLPPHEISTQIAPRDGYTELVSDLAIL
ASQLDRFALEIRELMRPEILELAEGSAESRVGSSTMPHKENPVTAEKISGLAKVLRGFVISELENIPLWHERDLTNSSSE
RIILSHSFLIMDEMLESMEALLRNLRVYPENMRKNLELTKGLIMAESLMINLTLANVPRHIAHELVMKVSREAEREGKSL
LEAALDNEEIVKILGKEKIIEALNPYSYLGEYKKLISRALSYAKNVIEQT

Specific function: De novo purine biosynthesis; eighth step. [C]

COG id: COG0015

COG function: function code F; Adenylosuccinate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lyase 1 family. Adenylosuccinate lyase subfamily [H]

Homologues:

Organism=Homo sapiens, GI4557269, Length=447, Percent_Identity=26.6219239373602, Blast_Score=151, Evalue=1e-36,
Organism=Homo sapiens, GI183227688, Length=405, Percent_Identity=27.4074074074074, Blast_Score=144, Evalue=2e-34,
Organism=Homo sapiens, GI19743875, Length=303, Percent_Identity=26.0726072607261, Blast_Score=69, Evalue=7e-12,
Organism=Escherichia coli, GI1787376, Length=343, Percent_Identity=27.9883381924198, Blast_Score=107, Evalue=2e-24,
Organism=Escherichia coli, GI1790398, Length=320, Percent_Identity=29.375, Blast_Score=82, Evalue=7e-17,
Organism=Caenorhabditis elegans, GI17508577, Length=361, Percent_Identity=27.4238227146814, Blast_Score=127, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI32564234, Length=313, Percent_Identity=28.1150159744409, Blast_Score=106, Evalue=3e-23,
Organism=Caenorhabditis elegans, GI17553882, Length=345, Percent_Identity=27.536231884058, Blast_Score=96, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI32565146, Length=340, Percent_Identity=27.9411764705882, Blast_Score=96, Evalue=3e-20,
Organism=Saccharomyces cerevisiae, GI6323391, Length=459, Percent_Identity=27.4509803921569, Blast_Score=142, Evalue=8e-35,
Organism=Saccharomyces cerevisiae, GI6321806, Length=374, Percent_Identity=25.4010695187166, Blast_Score=65, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24647570, Length=420, Percent_Identity=26.9047619047619, Blast_Score=132, Evalue=6e-31,
Organism=Drosophila melanogaster, GI221473854, Length=422, Percent_Identity=24.6445497630332, Blast_Score=89, Evalue=8e-18,
Organism=Drosophila melanogaster, GI78706858, Length=422, Percent_Identity=24.6445497630332, Blast_Score=89, Evalue=8e-18,
Organism=Drosophila melanogaster, GI24640179, Length=299, Percent_Identity=26.7558528428094, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24640177, Length=318, Percent_Identity=26.4150943396226, Blast_Score=84, Evalue=3e-16,
Organism=Drosophila melanogaster, GI78710009, Length=310, Percent_Identity=27.0967741935484, Blast_Score=81, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24583245, Length=345, Percent_Identity=24.3478260869565, Blast_Score=79, Evalue=7e-15,
Organism=Drosophila melanogaster, GI24662684, Length=289, Percent_Identity=27.3356401384083, Blast_Score=75, Evalue=8e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019468
- InterPro:   IPR003031
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761
- InterPro:   IPR004769 [H]

Pfam domain/function: PF10397 ADSL_C; PF00206 Lyase_1 [H]

EC number: =4.3.2.2 [H]

Molecular weight: Translated: 51086; Mature: 51086

Theoretical pI: Translated: 6.46; Mature: 6.46

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MENSVCVLDWRYGSKEMRELFQRATILKRMATVEVALLYALSKIGLVREEDVLVVQKNID
CCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCC
KINISRVEELEKKLGHDVMALTVHLAELSGESGKFIHFGATSYDIVDTANVLIFRDAINI
CCCHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCEEEECCCCCCHHCCCHHHHHHHHHHH
IKKKLKNIIIILMEYAEKYQELVMVGRTHGQHALPITLGFKFANYVYEFTRSLERLNDTS
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHH
KRLLKIKMAGAVGTMAGWKDKGLEIEKYVSEYLGLPPHEISTQIAPRDGYTELVSDLAIL
HHHHHHHHHCCHHHHCCCCCCCCCHHHHHHHHHCCCCHHHCCCCCCCCCHHHHHHHHHHH
ASQLDRFALEIRELMRPEILELAEGSAESRVGSSTMPHKENPVTAEKISGLAKVLRGFVI
HHHHHHHHHHHHHHHCHHHHHHHCCCCHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
SELENIPLWHERDLTNSSSERIILSHSFLIMDEMLESMEALLRNLRVYPENMRKNLELTK
HHHHCCCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
GLIMAESLMINLTLANVPRHIAHELVMKVSREAEREGKSLLEAALDNEEIVKILGKEKII
HHHHHHHHHEEEHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
EALNPYSYLGEYKKLISRALSYAKNVIEQT
HHHCHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MENSVCVLDWRYGSKEMRELFQRATILKRMATVEVALLYALSKIGLVREEDVLVVQKNID
CCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCC
KINISRVEELEKKLGHDVMALTVHLAELSGESGKFIHFGATSYDIVDTANVLIFRDAINI
CCCHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCEEEECCCCCCHHCCCHHHHHHHHHHH
IKKKLKNIIIILMEYAEKYQELVMVGRTHGQHALPITLGFKFANYVYEFTRSLERLNDTS
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHH
KRLLKIKMAGAVGTMAGWKDKGLEIEKYVSEYLGLPPHEISTQIAPRDGYTELVSDLAIL
HHHHHHHHHCCHHHHCCCCCCCCCHHHHHHHHHCCCCHHHCCCCCCCCCHHHHHHHHHHH
ASQLDRFALEIRELMRPEILELAEGSAESRVGSSTMPHKENPVTAEKISGLAKVLRGFVI
HHHHHHHHHHHHHHHCHHHHHHHCCCCHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
SELENIPLWHERDLTNSSSERIILSHSFLIMDEMLESMEALLRNLRVYPENMRKNLELTK
HHHHCCCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
GLIMAESLMINLTLANVPRHIAHELVMKVSREAEREGKSLLEAALDNEEIVKILGKEKII
HHHHHHHHHEEEHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
EALNPYSYLGEYKKLISRALSYAKNVIEQT
HHHCHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12622808 [H]