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The map label for this gene is rpoA2 [H]

Identifier: 15920464

GI number: 15920464

Start: 288078

End: 289274

Strand: Reverse

Name: rpoA2 [H]

Synonym: ST0275

Alternate gene names: 15920464

Gene position: 289274-288078 (Counterclockwise)

Preceding gene: 15920465

Following gene: 15920463

Centisome position: 10.73

GC content: 32.58

Gene sequence:

>1197_bases
ATGGAAGAGGTGAACTATATGGTTTCGGACTATATTAAATCTTATATAGAAAGATCACTAGAACAAATAAGGGATAAATT
ACCTGATAAAGTAATACAAGATTTAAGAGAAGCTTTATTAAATACTGATATAAATCTCACAGAATCTGATGTGGATAAAA
TAATAGATTTAGCTGTGAAAGATTACCAAGAGTCGTTAATTGAACCTGGAGAAGCTGTAGGTATTGTATCAGCACAGTCA
ATAGGCGAGCCTGGTACACAAATGACTTTAAGAACCTTCCACTTTGCCGGTATTAGAGAGTTAAACGTTACTTTGGGTTT
ACCCCGATTAATAGAGATTGTTGACGCTAGAAAAACACCATCTACCCCTATAATGACTATCTATTTAACAGATGAATATA
AATACGATAAAGAAAAAGCATTGGAAATAGCAAGAAAAATTGAGTATACCAAAATAGAGAATGTGATTTCATCTATAAGT
GTTGATATAACTAATATGTCTATTACCATACAATTAGACGAGGAGATGCTTAAAGATAAAGGAGTAGATCCTTCTAGTGT
TAAGAAGATCATCTCGAAGTTAAAATTAGGTAAGATACGAATTGAAGATATTGACGATTATACTTTTACCATTTACTTTG
AGGAAATAGATAATATTTCAGCTCTGTTTAAAATGCGTGAAAAGTTATTAAATACAAAAATTAAAGGAATAAAAGGAATT
AGAAGAGCTATTGTACAGAAAAAAGGAGATGAATATGTAATTATAACTGATGGTTCAAACTTAGAAGGCGTAATAGGAAT
AAAGGGTGTTGATGTAAATAAAATTCAGACAAATAATATTCATGAGATAGCAGACACTTTTGGAATAGAGGCTGCAAGAG
AGGCTATTGCAAGAGAGATTAAAAAAGTTCTTGAAGAACAAGGTCTAGATGTAGATATGAGACATATTCTATTAGTAGCA
GATATAATGACAAGAACTGGTGTAGTTAGACAAATTGGAAGACATGGAGTAACTGGAGAAAAAAGTAGCGTATTAGCTAG
AGCTGCATTCGAGGTAACTGTGAAACATTTACTTGATGCTGCAGCAAGAGGAGAAATGGAAGAATTTAAAGGAGTAGTAG
AAAATATTATAATCGGTCAACCAATTAGATTGGGCACGGGTATAGTTGAGTTGACTATGAGGCTGAATGTGAGGTGA

Upstream 100 bases:

>100_bases
AAGTAATCGAACTAGCTTATGGTGAAGACGAGGTGCATCCAATGTATAGTTCTCACGGTAAAACTGTTGACGTAAATAGA
ATAATAGAAAGAGTAGTAGG

Downstream 100 bases:

>100_bases
AGTAAATGGCAGAAGTAACAGGATTTGAAAGCGAGCTAAAAACTTTATTAAGAACAGGAAAAGTAGTTTTTGGGAGCAAA
AAAACAATAAAAATGGTGAA

Product: DNA-directed RNA polymerase subunit A''

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 398; Mature: 398

Protein sequence:

>398_residues
MEEVNYMVSDYIKSYIERSLEQIRDKLPDKVIQDLREALLNTDINLTESDVDKIIDLAVKDYQESLIEPGEAVGIVSAQS
IGEPGTQMTLRTFHFAGIRELNVTLGLPRLIEIVDARKTPSTPIMTIYLTDEYKYDKEKALEIARKIEYTKIENVISSIS
VDITNMSITIQLDEEMLKDKGVDPSSVKKIISKLKLGKIRIEDIDDYTFTIYFEEIDNISALFKMREKLLNTKIKGIKGI
RRAIVQKKGDEYVIITDGSNLEGVIGIKGVDVNKIQTNNIHEIADTFGIEAAREAIAREIKKVLEEQGLDVDMRHILLVA
DIMTRTGVVRQIGRHGVTGEKSSVLARAAFEVTVKHLLDAAARGEMEEFKGVVENIIIGQPIRLGTGIVELTMRLNVR

Sequences:

>Translated_398_residues
MEEVNYMVSDYIKSYIERSLEQIRDKLPDKVIQDLREALLNTDINLTESDVDKIIDLAVKDYQESLIEPGEAVGIVSAQS
IGEPGTQMTLRTFHFAGIRELNVTLGLPRLIEIVDARKTPSTPIMTIYLTDEYKYDKEKALEIARKIEYTKIENVISSIS
VDITNMSITIQLDEEMLKDKGVDPSSVKKIISKLKLGKIRIEDIDDYTFTIYFEEIDNISALFKMREKLLNTKIKGIKGI
RRAIVQKKGDEYVIITDGSNLEGVIGIKGVDVNKIQTNNIHEIADTFGIEAAREAIAREIKKVLEEQGLDVDMRHILLVA
DIMTRTGVVRQIGRHGVTGEKSSVLARAAFEVTVKHLLDAAARGEMEEFKGVVENIIIGQPIRLGTGIVELTMRLNVR
>Mature_398_residues
MEEVNYMVSDYIKSYIERSLEQIRDKLPDKVIQDLREALLNTDINLTESDVDKIIDLAVKDYQESLIEPGEAVGIVSAQS
IGEPGTQMTLRTFHFAGIRELNVTLGLPRLIEIVDARKTPSTPIMTIYLTDEYKYDKEKALEIARKIEYTKIENVISSIS
VDITNMSITIQLDEEMLKDKGVDPSSVKKIISKLKLGKIRIEDIDDYTFTIYFEEIDNISALFKMREKLLNTKIKGIKGI
RRAIVQKKGDEYVIITDGSNLEGVIGIKGVDVNKIQTNNIHEIADTFGIEAAREAIAREIKKVLEEQGLDVDMRHILLVA
DIMTRTGVVRQIGRHGVTGEKSSVLARAAFEVTVKHLLDAAARGEMEEFKGVVENIIIGQPIRLGTGIVELTMRLNVR

Specific function: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates [H]

COG id: COG0086

COG function: function code K; DNA-directed RNA polymerase, beta' subunit/160 kD subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA polymerase beta' chain family [H]

Homologues:

Organism=Homo sapiens, GI39725938, Length=369, Percent_Identity=35.230352303523, Blast_Score=204, Evalue=1e-52,
Organism=Homo sapiens, GI4505939, Length=403, Percent_Identity=30.5210918114144, Blast_Score=185, Evalue=5e-47,
Organism=Homo sapiens, GI103471997, Length=168, Percent_Identity=36.3095238095238, Blast_Score=95, Evalue=9e-20,
Organism=Caenorhabditis elegans, GI25145495, Length=384, Percent_Identity=34.1145833333333, Blast_Score=202, Evalue=3e-52,
Organism=Caenorhabditis elegans, GI71987878, Length=403, Percent_Identity=31.5136476426799, Blast_Score=191, Evalue=6e-49,
Organism=Caenorhabditis elegans, GI71998295, Length=64, Percent_Identity=64.0625, Blast_Score=84, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6324690, Length=398, Percent_Identity=33.1658291457286, Blast_Score=196, Evalue=6e-51,
Organism=Saccharomyces cerevisiae, GI6320061, Length=409, Percent_Identity=32.0293398533007, Blast_Score=193, Evalue=3e-50,
Organism=Saccharomyces cerevisiae, GI6324917, Length=106, Percent_Identity=48.1132075471698, Blast_Score=89, Evalue=1e-18,
Organism=Drosophila melanogaster, GI281360912, Length=354, Percent_Identity=38.135593220339, Blast_Score=211, Evalue=1e-54,
Organism=Drosophila melanogaster, GI17530899, Length=403, Percent_Identity=31.5136476426799, Blast_Score=202, Evalue=3e-52,
Organism=Drosophila melanogaster, GI17647875, Length=173, Percent_Identity=35.2601156069364, Blast_Score=104, Evalue=9e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007081
- InterPro:   IPR012757 [H]

Pfam domain/function: PF04998 RNA_pol_Rpb1_5 [H]

EC number: =2.7.7.6 [H]

Molecular weight: Translated: 44761; Mature: 44761

Theoretical pI: Translated: 5.01; Mature: 5.01

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEEVNYMVSDYIKSYIERSLEQIRDKLPDKVIQDLREALLNTDINLTESDVDKIIDLAVK
CCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHH
DYQESLIEPGEAVGIVSAQSIGEPGTQMTLRTFHFAGIRELNVTLGLPRLIEIVDARKTP
HHHHHHHCCCCCEEEEEHHHCCCCCCHHEEHHHHHCCCEEEEEEECCHHHHHHHHCCCCC
STPIMTIYLTDEYKYDKEKALEIARKIEYTKIENVISSISVDITNMSITIQLDEEMLKDK
CCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEEEEEEECHHHHHHC
GVDPSSVKKIISKLKLGKIRIEDIDDYTFTIYFEEIDNISALFKMREKLLNTKIKGIKGI
CCCHHHHHHHHHHHCCCCEEEEECCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHH
RRAIVQKKGDEYVIITDGSNLEGVIGIKGVDVNKIQTNNIHEIADTFGIEAAREAIAREI
HHHHHHCCCCCEEEEECCCCCCEEEEECCCCCCCEECCCHHHHHHHHCHHHHHHHHHHHH
KKVLEEQGLDVDMRHILLVADIMTRTGVVRQIGRHGVTGEKSSVLARAAFEVTVKHLLDA
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHH
AARGEMEEFKGVVENIIIGQPIRLGTGIVELTMRLNVR
HHCCCHHHHHHHHHHHHCCCCHHHCCCCEEEEEEECCC
>Mature Secondary Structure
MEEVNYMVSDYIKSYIERSLEQIRDKLPDKVIQDLREALLNTDINLTESDVDKIIDLAVK
CCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHH
DYQESLIEPGEAVGIVSAQSIGEPGTQMTLRTFHFAGIRELNVTLGLPRLIEIVDARKTP
HHHHHHHCCCCCEEEEEHHHCCCCCCHHEEHHHHHCCCEEEEEEECCHHHHHHHHCCCCC
STPIMTIYLTDEYKYDKEKALEIARKIEYTKIENVISSISVDITNMSITIQLDEEMLKDK
CCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEEEEEEECHHHHHHC
GVDPSSVKKIISKLKLGKIRIEDIDDYTFTIYFEEIDNISALFKMREKLLNTKIKGIKGI
CCCHHHHHHHHHHHCCCCEEEEECCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHH
RRAIVQKKGDEYVIITDGSNLEGVIGIKGVDVNKIQTNNIHEIADTFGIEAAREAIAREI
HHHHHHCCCCCEEEEECCCCCCEEEEECCCCCCCEECCCHHHHHHHHCHHHHHHHHHHHH
KKVLEEQGLDVDMRHILLVADIMTRTGVVRQIGRHGVTGEKSSVLARAAFEVTVKHLLDA
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHH
AARGEMEEFKGVVENIIIGQPIRLGTGIVELTMRLNVR
HHCCCHHHHHHHHHHHHCCCCHHHCCCCEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11572479 [H]