Click here to switch to the map view.

The map label for this gene is tuf

Identifier: 15920458

GI number: 15920458

Start: 284348

End: 285655

Strand: Reverse

Name: tuf

Synonym: ST0269

Alternate gene names: 15920458

Gene position: 285655-284348 (Counterclockwise)

Preceding gene: 15920459

Following gene: 15920457

Centisome position: 10.6

GC content: 35.63

Gene sequence:

>1308_bases
ATGTCACAAAAGCCACACCTTAATTTAATTGTTATAGGACATGTAGACCATGGTAAAAGCACCTTAGTTGGAAGACTTTT
GATGGATAGAGGTTTTCTAGATGAAAAAACAATAAAAGAAGCAGAAGAAGCTGCAAAGAAACTAGGGAAAGAATCAGAAA
AGTATGCCTTCTTACTAGACAGATTAAAAGAAGAAAGAGAAAGAGGTGTAACGATAAACTTAACCTTTATGAGATTCGAG
ACAAAGAAGTTCTTCTTTACAATTATTGACGCACCTGGTCATAGAGACTTCGTAAAGAATATGATAACTGGTGCAAGCCA
AGCGGATGCTGCTATATTAGTAGTCTCAGCTAAGAAAGGTGAATATGAAGCTGGTATGAGTGCAGAAGGACAAACAAGAG
AACACATAATACTAGCAAAAACAATGGGAATAAATCAAGTGATAGTAGCTGTAAATAAAATGGATCTAACTGATCCACCT
TATGATGAGAAAAGATTCAAAGAAATTGTAGATCAAGTAGGTAAGTTTATGAAAAGCTTTGGATTTGATATGAACAAGGT
TAAGTTTGTACCAGTAGTTGCGCCAACTGGTGAAAATATAACACAGAGATCTGAAAATATGAAATGGTATACTGGTCCAA
CATTAGAAGAATATTTAGATCAACTAGAGATACCGCCAAAACCAGTTGATAAGCCATTAAGGATACCAATTCAAGAAGTA
TACTCAATTTCTGGTGTAGGTGTAGTCCCAGTAGGTAGAGTAGAAACTGGTGTCCTTAAAGTAGGTGATAAAGTAGTATT
TATGCCAGTAGGAAAGGTTGGTGAAGTTAGATCTATTGAAACTCATCATACAAAAATTGAAAAAGCGGAACCAGGTGATA
ATATTGGATTCAACGTTAGAGGTGTTGAAAAGAAAGATATCAAAAGAGGAGATGTTGCTGGTAGCTTAAATGTTCCACCT
ACTGTTGCAGACGAATTTACAGCACAAATAATAGTAATATGGCACCCAACAGCTGTAAGTGTTGGTTATACCCCAGTAGT
TCATATACATACAGCTAGTGTAGCTTGTAGAATAACAGAACTCACTTCAAAGATTGATCCAAAGACCGGTAAGGAGGTTG
AAAAGAACCCACAATTCCTAAAGTCTGGTGATTCAGCAATAGTAAAAATGAAGCCTATAAAAGAGTTAGTTGTAGAGAAA
TTCAGAGAATTCCCAGCATTAGGAAGATTTGCTATGAGAGATATGGGTAAAACAGTGGGAGTTGGAGTAGTTATTGATGT
AAAACCAAAGAAGGTAGAGATTAAATAA

Upstream 100 bases:

>100_bases
AGAAAGAAAGAAGAAATTGAGAGAATTGCATTAAGCTCGAGATAACTTATAGCTAAACTTTTAATGCTTGTTACGTTAAT
TATGTGCAAGAGGGAGTAAG

Downstream 100 bases:

>100_bases
TAAGATTTTATATTTTTTATATGGTGATTTTATGCCTACTAAGGCGAGAATTAGACTTTGGAGTACTAATATAGAGAATT
TGAACTTTGTAGTGAATCAA

Product: elongation factor 1-alpha

Products: GDP; phosphate

Alternate protein names: EF-1-alpha; Elongation factor Tu; EF-Tu

Number of amino acids: Translated: 435; Mature: 434

Protein sequence:

>435_residues
MSQKPHLNLIVIGHVDHGKSTLVGRLLMDRGFLDEKTIKEAEEAAKKLGKESEKYAFLLDRLKEERERGVTINLTFMRFE
TKKFFFTIIDAPGHRDFVKNMITGASQADAAILVVSAKKGEYEAGMSAEGQTREHIILAKTMGINQVIVAVNKMDLTDPP
YDEKRFKEIVDQVGKFMKSFGFDMNKVKFVPVVAPTGENITQRSENMKWYTGPTLEEYLDQLEIPPKPVDKPLRIPIQEV
YSISGVGVVPVGRVETGVLKVGDKVVFMPVGKVGEVRSIETHHTKIEKAEPGDNIGFNVRGVEKKDIKRGDVAGSLNVPP
TVADEFTAQIIVIWHPTAVSVGYTPVVHIHTASVACRITELTSKIDPKTGKEVEKNPQFLKSGDSAIVKMKPIKELVVEK
FREFPALGRFAMRDMGKTVGVGVVIDVKPKKVEIK

Sequences:

>Translated_435_residues
MSQKPHLNLIVIGHVDHGKSTLVGRLLMDRGFLDEKTIKEAEEAAKKLGKESEKYAFLLDRLKEERERGVTINLTFMRFE
TKKFFFTIIDAPGHRDFVKNMITGASQADAAILVVSAKKGEYEAGMSAEGQTREHIILAKTMGINQVIVAVNKMDLTDPP
YDEKRFKEIVDQVGKFMKSFGFDMNKVKFVPVVAPTGENITQRSENMKWYTGPTLEEYLDQLEIPPKPVDKPLRIPIQEV
YSISGVGVVPVGRVETGVLKVGDKVVFMPVGKVGEVRSIETHHTKIEKAEPGDNIGFNVRGVEKKDIKRGDVAGSLNVPP
TVADEFTAQIIVIWHPTAVSVGYTPVVHIHTASVACRITELTSKIDPKTGKEVEKNPQFLKSGDSAIVKMKPIKELVVEK
FREFPALGRFAMRDMGKTVGVGVVIDVKPKKVEIK
>Mature_434_residues
SQKPHLNLIVIGHVDHGKSTLVGRLLMDRGFLDEKTIKEAEEAAKKLGKESEKYAFLLDRLKEERERGVTINLTFMRFET
KKFFFTIIDAPGHRDFVKNMITGASQADAAILVVSAKKGEYEAGMSAEGQTREHIILAKTMGINQVIVAVNKMDLTDPPY
DEKRFKEIVDQVGKFMKSFGFDMNKVKFVPVVAPTGENITQRSENMKWYTGPTLEEYLDQLEIPPKPVDKPLRIPIQEVY
SISGVGVVPVGRVETGVLKVGDKVVFMPVGKVGEVRSIETHHTKIEKAEPGDNIGFNVRGVEKKDIKRGDVAGSLNVPPT
VADEFTAQIIVIWHPTAVSVGYTPVVHIHTASVACRITELTSKIDPKTGKEVEKNPQFLKSGDSAIVKMKPIKELVVEKF
REFPALGRFAMRDMGKTVGVGVVIDVKPKKVEIK

Specific function: This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis

COG id: COG5256

COG function: function code J; Translation elongation factor EF-1alpha (GTPase)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily

Homologues:

Organism=Homo sapiens, GI4503471, Length=441, Percent_Identity=53.2879818594104, Blast_Score=488, Evalue=1e-138,
Organism=Homo sapiens, GI4503475, Length=441, Percent_Identity=52.1541950113379, Blast_Score=480, Evalue=1e-135,
Organism=Homo sapiens, GI223555963, Length=428, Percent_Identity=38.785046728972, Blast_Score=329, Evalue=3e-90,
Organism=Homo sapiens, GI5729864, Length=428, Percent_Identity=38.785046728972, Blast_Score=329, Evalue=3e-90,
Organism=Homo sapiens, GI194018520, Length=430, Percent_Identity=37.906976744186, Blast_Score=315, Evalue=5e-86,
Organism=Homo sapiens, GI194018522, Length=430, Percent_Identity=37.906976744186, Blast_Score=315, Evalue=7e-86,
Organism=Homo sapiens, GI194097354, Length=430, Percent_Identity=37.906976744186, Blast_Score=314, Evalue=1e-85,
Organism=Homo sapiens, GI46094014, Length=430, Percent_Identity=37.906976744186, Blast_Score=312, Evalue=3e-85,
Organism=Homo sapiens, GI34147630, Length=438, Percent_Identity=31.2785388127854, Blast_Score=176, Evalue=5e-44,
Organism=Homo sapiens, GI239752724, Length=152, Percent_Identity=35.5263157894737, Blast_Score=97, Evalue=3e-20,
Organism=Homo sapiens, GI169162126, Length=152, Percent_Identity=35.5263157894737, Blast_Score=97, Evalue=3e-20,
Organism=Homo sapiens, GI169162824, Length=152, Percent_Identity=35.5263157894737, Blast_Score=97, Evalue=3e-20,
Organism=Homo sapiens, GI54607086, Length=340, Percent_Identity=24.7058823529412, Blast_Score=94, Evalue=4e-19,
Organism=Homo sapiens, GI82546879, Length=414, Percent_Identity=24.3961352657005, Blast_Score=89, Evalue=5e-18,
Organism=Homo sapiens, GI45593140, Length=452, Percent_Identity=24.5575221238938, Blast_Score=88, Evalue=2e-17,
Organism=Homo sapiens, GI157426893, Length=318, Percent_Identity=27.3584905660377, Blast_Score=83, Evalue=5e-16,
Organism=Escherichia coli, GI1789108, Length=435, Percent_Identity=33.1034482758621, Blast_Score=195, Evalue=6e-51,
Organism=Escherichia coli, GI1789737, Length=430, Percent_Identity=30, Blast_Score=166, Evalue=2e-42,
Organism=Escherichia coli, GI1790412, Length=430, Percent_Identity=30, Blast_Score=166, Evalue=2e-42,
Organism=Escherichia coli, GI2367247, Length=379, Percent_Identity=28.2321899736148, Blast_Score=122, Evalue=5e-29,
Organism=Escherichia coli, GI48994988, Length=326, Percent_Identity=26.6871165644172, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI1788922, Length=271, Percent_Identity=28.7822878228782, Blast_Score=80, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI17552884, Length=439, Percent_Identity=51.4806378132118, Blast_Score=467, Evalue=1e-132,
Organism=Caenorhabditis elegans, GI17569207, Length=439, Percent_Identity=51.4806378132118, Blast_Score=467, Evalue=1e-132,
Organism=Caenorhabditis elegans, GI32566303, Length=439, Percent_Identity=47.1526195899772, Blast_Score=407, Evalue=1e-114,
Organism=Caenorhabditis elegans, GI32566629, Length=431, Percent_Identity=37.3549883990719, Blast_Score=303, Evalue=8e-83,
Organism=Caenorhabditis elegans, GI115532067, Length=429, Percent_Identity=38.4615384615385, Blast_Score=283, Evalue=2e-76,
Organism=Caenorhabditis elegans, GI115532065, Length=429, Percent_Identity=38.4615384615385, Blast_Score=282, Evalue=3e-76,
Organism=Caenorhabditis elegans, GI32566301, Length=146, Percent_Identity=57.5342465753425, Blast_Score=189, Evalue=4e-48,
Organism=Caenorhabditis elegans, GI17556456, Length=437, Percent_Identity=32.0366132723112, Blast_Score=169, Evalue=2e-42,
Organism=Caenorhabditis elegans, GI25141371, Length=323, Percent_Identity=32.1981424148607, Blast_Score=139, Evalue=4e-33,
Organism=Caenorhabditis elegans, GI17565438, Length=445, Percent_Identity=24.0449438202247, Blast_Score=86, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI17557151, Length=258, Percent_Identity=28.2945736434109, Blast_Score=83, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI17506081, Length=302, Percent_Identity=25.4966887417219, Blast_Score=81, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17509919, Length=337, Percent_Identity=21.6617210682493, Blast_Score=70, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI32566299, Length=61, Percent_Identity=50.8196721311475, Blast_Score=68, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6325337, Length=438, Percent_Identity=51.5981735159817, Blast_Score=462, Evalue=1e-131,
Organism=Saccharomyces cerevisiae, GI6319594, Length=438, Percent_Identity=51.5981735159817, Blast_Score=462, Evalue=1e-131,
Organism=Saccharomyces cerevisiae, GI6320377, Length=428, Percent_Identity=36.6822429906542, Blast_Score=278, Evalue=8e-76,
Organism=Saccharomyces cerevisiae, GI6322937, Length=450, Percent_Identity=31.5555555555556, Blast_Score=225, Evalue=1e-59,
Organism=Saccharomyces cerevisiae, GI6324761, Length=439, Percent_Identity=30.9794988610478, Blast_Score=176, Evalue=5e-45,
Organism=Saccharomyces cerevisiae, GI6323320, Length=282, Percent_Identity=26.9503546099291, Blast_Score=79, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24652838, Length=435, Percent_Identity=52.4137931034483, Blast_Score=474, Evalue=1e-134,
Organism=Drosophila melanogaster, GI17137572, Length=435, Percent_Identity=52.4137931034483, Blast_Score=474, Evalue=1e-134,
Organism=Drosophila melanogaster, GI45553807, Length=442, Percent_Identity=52.0361990950226, Blast_Score=472, Evalue=1e-133,
Organism=Drosophila melanogaster, GI45553816, Length=442, Percent_Identity=52.0361990950226, Blast_Score=472, Evalue=1e-133,
Organism=Drosophila melanogaster, GI24651721, Length=442, Percent_Identity=52.0361990950226, Blast_Score=472, Evalue=1e-133,
Organism=Drosophila melanogaster, GI17864154, Length=442, Percent_Identity=52.0361990950226, Blast_Score=472, Evalue=1e-133,
Organism=Drosophila melanogaster, GI45550900, Length=429, Percent_Identity=36.5967365967366, Blast_Score=310, Evalue=2e-84,
Organism=Drosophila melanogaster, GI17137380, Length=429, Percent_Identity=35.4312354312354, Blast_Score=290, Evalue=9e-79,
Organism=Drosophila melanogaster, GI281363316, Length=444, Percent_Identity=31.5315315315315, Blast_Score=186, Evalue=3e-47,
Organism=Drosophila melanogaster, GI17864358, Length=444, Percent_Identity=31.5315315315315, Blast_Score=186, Evalue=3e-47,
Organism=Drosophila melanogaster, GI19921738, Length=439, Percent_Identity=26.879271070615, Blast_Score=154, Evalue=8e-38,
Organism=Drosophila melanogaster, GI19922690, Length=314, Percent_Identity=25.4777070063694, Blast_Score=96, Evalue=5e-20,
Organism=Drosophila melanogaster, GI19922512, Length=444, Percent_Identity=22.7477477477477, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24654852, Length=444, Percent_Identity=22.7477477477477, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24647048, Length=367, Percent_Identity=22.6158038147139, Blast_Score=75, Evalue=9e-14,
Organism=Drosophila melanogaster, GI24647046, Length=366, Percent_Identity=22.4043715846995, Blast_Score=75, Evalue=9e-14,
Organism=Drosophila melanogaster, GI78706572, Length=280, Percent_Identity=27.1428571428571, Blast_Score=70, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): EF1A_SULTO (Q976B1)

Other databases:

- EMBL:   BA000023
- RefSeq:   NP_376127.1
- ProteinModelPortal:   Q976B1
- SMR:   Q976B1
- GeneID:   1458170
- GenomeReviews:   BA000023_GR
- KEGG:   sto:ST0269
- NMPDR:   fig|273063.1.peg.295
- HOGENOM:   HBG307581
- OMA:   ETFQAQI
- ProtClustDB:   PRK12317
- BioCyc:   STOK273063:ST0269-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00118_A
- InterPro:   IPR000795
- InterPro:   IPR009001
- InterPro:   IPR004539
- InterPro:   IPR004161
- InterPro:   IPR004160
- InterPro:   IPR009000
- PRINTS:   PR00315
- TIGRFAMs:   TIGR00483

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF03143 GTP_EFTU_D3; SSF50465 Elong_init_C; SSF50447 Translat_factor

EC number: 3.6.5.3

Molecular weight: Translated: 48332; Mature: 48201

Theoretical pI: Translated: 9.67; Mature: 9.67

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQKPHLNLIVIGHVDHGKSTLVGRLLMDRGFLDEKTIKEAEEAAKKLGKESEKYAFLLD
CCCCCCEEEEEEEECCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHH
RLKEERERGVTINLTFMRFETKKFFFTIIDAPGHRDFVKNMITGASQADAAILVVSAKKG
HHHHHHHCCCEEEEEEEEEECCEEEEEEEECCCCHHHHHHHHCCCCCCCEEEEEEEECCC
EYEAGMSAEGQTREHIILAKTMGINQVIVAVNKMDLTDPPYDEKRFKEIVDQVGKFMKSF
CCCCCCCCCCCCCCEEEEEEECCCCEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHH
GFDMNKVKFVPVVAPTGENITQRSENMKWYTGPTLEEYLDQLEIPPKPVDKPLRIPIQEV
CCCCCCEEEEEEEECCCCCHHHHCCCCEEEECCCHHHHHHHCCCCCCCCCCCCCCCHHHH
YSISGVGVVPVGRVETGVLKVGDKVVFMPVGKVGEVRSIETHHTKIEKAEPGDNIGFNVR
HCCCCCCEEECCCCCCCEEEECCEEEEEECCCCCCCCEEEHHHCEEECCCCCCCCCEEEE
GVEKKDIKRGDVAGSLNVPPTVADEFTAQIIVIWHPTAVSVGYTPVVHIHTASVACRITE
CCCHHHCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCEEECCCCEEEEEEECCCEEEEEH
LTSKIDPKTGKEVEKNPQFLKSGDSAIVKMKPIKELVVEKFREFPALGRFAMRDMGKTVG
HHHHCCCCCCCCCCCCCHHHHCCCCEEEEECHHHHHHHHHHHHCCHHHHHHHHHCCCCCC
VGVVIDVKPKKVEIK
EEEEEEECCCEEECC
>Mature Secondary Structure 
SQKPHLNLIVIGHVDHGKSTLVGRLLMDRGFLDEKTIKEAEEAAKKLGKESEKYAFLLD
CCCCCEEEEEEEECCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHH
RLKEERERGVTINLTFMRFETKKFFFTIIDAPGHRDFVKNMITGASQADAAILVVSAKKG
HHHHHHHCCCEEEEEEEEEECCEEEEEEEECCCCHHHHHHHHCCCCCCCEEEEEEEECCC
EYEAGMSAEGQTREHIILAKTMGINQVIVAVNKMDLTDPPYDEKRFKEIVDQVGKFMKSF
CCCCCCCCCCCCCCEEEEEEECCCCEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHH
GFDMNKVKFVPVVAPTGENITQRSENMKWYTGPTLEEYLDQLEIPPKPVDKPLRIPIQEV
CCCCCCEEEEEEEECCCCCHHHHCCCCEEEECCCHHHHHHHCCCCCCCCCCCCCCCHHHH
YSISGVGVVPVGRVETGVLKVGDKVVFMPVGKVGEVRSIETHHTKIEKAEPGDNIGFNVR
HCCCCCCEEECCCCCCCEEEECCEEEEEECCCCCCCCEEEHHHCEEECCCCCCCCCEEEE
GVEKKDIKRGDVAGSLNVPPTVADEFTAQIIVIWHPTAVSVGYTPVVHIHTASVACRITE
CCCHHHCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCEEECCCCEEEEEEECCCEEEEEH
LTSKIDPKTGKEVEKNPQFLKSGDSAIVKMKPIKELVVEKFREFPALGRFAMRDMGKTVG
HHHHCCCCCCCCCCCCCHHHHCCCCEEEEECHHHHHHHHHHHHCCHHHHHHHHHCCCCCC
VGVVIDVKPKKVEIK
EEEEEEECCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GTP; H2O

Specific reaction: GTP + H2O = GDP + phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11572479