Click here to switch to the map view.

The map label for this gene is lysX

Identifier: 15920370

GI number: 15920370

Start: 215433

End: 216290

Strand: Reverse

Name: lysX

Synonym: ST0192

Alternate gene names: 15920370

Gene position: 216290-215433 (Counterclockwise)

Preceding gene: 15920371

Following gene: 15920369

Centisome position: 8.03

GC content: 29.95

Gene sequence:

>858_bases
GTGATCTTAGGGGTAATATATGATCTCCTAAGATGGGAAGAAAAAAATCTTATACAAGAAGCGAGGAAATTAGGACATAC
TGTAATTCCAATATATACTAAAGATTTTTATTATTTTTATAATAATGATAGTAATGAAACATTAGGAGATCTTGATGTTG
TGATACAAAGAAATACTTCTCATGCTAGAGCAGTAATAACATCCACTATATTTGAAAACTTAAGTTACAAAACTATTAAT
GACTCCTCAACTTTAATAAAATGTGAAAACAAACTTTACACCTTATCGTTATTATCAAAGCATGGGATAAGAGTTCCTAA
AACAATAGTAGCTTTTTCTAAAGAAAAAGCCTTAGAACTTGCAAATAAACTAAGTTATCCTGTTGTAATAAAGCCAGTTG
AAGGTAGCTGGGGTAGGATGGTAGCTAGAGCCATTGATGAGGATACATTAAGGAACTTTTTAGAGTATCAAGAATATACT
ACGTTACAATTTAGATATATTTATTTAATACAAGAATTTGTGAAAAAACCTGATAGGGACATAAGAATATTCACTATAGG
TGATGAGGCACCAGTTGGAATTTATAGAGTGAATTCGAGGAATTGGAAAACAAACACAGCTTTAGGTGCTAAGGCAGAAC
CTTTAAAGATAGACGAAGAATTACAAGATCTTGCATTAAAAGTAAAAGATATAATAGGAGGATTCTTTCTAGGTATCGAT
GTATTTGAAGATCCAGAAAGAGGTTATATAATTAATGAAGTAAATGGAGTGCCAGAATACAAAAATACTGTAAGAGTCAA
TAATTTTAACGTATCAGAGTATTTAATTAGAAAAATTGAAGAGTGGATTAAAAAATGA

Upstream 100 bases:

>100_bases
AAATAGTTGAGCATGAATGTGGAGCCCAATTAGAAGTATATAATGATCATGGAAGACTCGCACTAAGATTAGCCGAGCAA
GTAGGAGAGGACTGGGGAGA

Downstream 100 bases:

>100_bases
AGTTCATTCAACTTTATGGAGATAGAGGACTAACTATAGTAAAAGGAGAGGGTCAGTACGTATGGGATATTAGTGGAACA
AAATACTTAGACCTTCATAC

Product: hypothetical protein

Products: NA

Alternate protein names: AAA--lysW ligase lysX

Number of amino acids: Translated: 285; Mature: 285

Protein sequence:

>285_residues
MILGVIYDLLRWEEKNLIQEARKLGHTVIPIYTKDFYYFYNNDSNETLGDLDVVIQRNTSHARAVITSTIFENLSYKTIN
DSSTLIKCENKLYTLSLLSKHGIRVPKTIVAFSKEKALELANKLSYPVVIKPVEGSWGRMVARAIDEDTLRNFLEYQEYT
TLQFRYIYLIQEFVKKPDRDIRIFTIGDEAPVGIYRVNSRNWKTNTALGAKAEPLKIDEELQDLALKVKDIIGGFFLGID
VFEDPERGYIINEVNGVPEYKNTVRVNNFNVSEYLIRKIEEWIKK

Sequences:

>Translated_285_residues
MILGVIYDLLRWEEKNLIQEARKLGHTVIPIYTKDFYYFYNNDSNETLGDLDVVIQRNTSHARAVITSTIFENLSYKTIN
DSSTLIKCENKLYTLSLLSKHGIRVPKTIVAFSKEKALELANKLSYPVVIKPVEGSWGRMVARAIDEDTLRNFLEYQEYT
TLQFRYIYLIQEFVKKPDRDIRIFTIGDEAPVGIYRVNSRNWKTNTALGAKAEPLKIDEELQDLALKVKDIIGGFFLGID
VFEDPERGYIINEVNGVPEYKNTVRVNNFNVSEYLIRKIEEWIKK
>Mature_285_residues
MILGVIYDLLRWEEKNLIQEARKLGHTVIPIYTKDFYYFYNNDSNETLGDLDVVIQRNTSHARAVITSTIFENLSYKTIN
DSSTLIKCENKLYTLSLLSKHGIRVPKTIVAFSKEKALELANKLSYPVVIKPVEGSWGRMVARAIDEDTLRNFLEYQEYT
TLQFRYIYLIQEFVKKPDRDIRIFTIGDEAPVGIYRVNSRNWKTNTALGAKAEPLKIDEELQDLALKVKDIIGGFFLGID
VFEDPERGYIINEVNGVPEYKNTVRVNNFNVSEYLIRKIEEWIKK

Specific function: Catalyzes the ATP-dependent binding between the amino group of alpha-aminoadipate (AAA) and the gamma-carboxyl group of the C-terminal glutamate residue of lysW

COG id: COG0189

COG function: function code HJ; Glutathione synthase/Ribosomal protein S6 modification enzyme (glutaminyl transferase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain

Homologues:

Organism=Homo sapiens, GI164663810, Length=190, Percent_Identity=27.3684210526316, Blast_Score=77, Evalue=1e-14,
Organism=Homo sapiens, GI134254462, Length=191, Percent_Identity=25.130890052356, Blast_Score=66, Evalue=3e-11,
Organism=Escherichia coli, GI1787076, Length=216, Percent_Identity=26.8518518518519, Blast_Score=78, Evalue=5e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LYSX_SULTO (Q976J9)

Other databases:

- EMBL:   BA000023
- RefSeq:   NP_376039.1
- ProteinModelPortal:   Q976J9
- GeneID:   1458076
- GenomeReviews:   BA000023_GR
- KEGG:   sto:ST0192
- NMPDR:   fig|273063.1.peg.207
- HOGENOM:   HBG679591
- OMA:   HDKYLTY
- ProtClustDB:   CLSK883608
- BioCyc:   STOK273063:ST0192-MONOMER
- InterPro:   IPR011761
- InterPro:   IPR013651
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR011870
- InterPro:   IPR016185
- InterPro:   IPR004666
- Gene3D:   G3DSA:3.30.1490.20
- Gene3D:   G3DSA:3.30.470.20
- TIGRFAMs:   TIGR02144
- TIGRFAMs:   TIGR00768

Pfam domain/function: PF08443 RimK; SSF52440 PreATP-grasp-like

EC number: NA

Molecular weight: Translated: 33107; Mature: 33107

Theoretical pI: Translated: 6.81; Mature: 6.81

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
1.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MILGVIYDLLRWEEKNLIQEARKLGHTVIPIYTKDFYYFYNNDSNETLGDLDVVIQRNTS
CHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEEEEEEEECCCCCCCCCEEEEEECCCC
HARAVITSTIFENLSYKTINDSSTLIKCENKLYTLSLLSKHGIRVPKTIVAFSKEKALEL
HHHHHHHHHHHHCCCCEEECCCCCEEEECCCEEEEEEHHHCCCCCCHHHHHHHHHHHHHH
ANKLSYPVVIKPVEGSWGRMVARAIDEDTLRNFLEYQEYTTLQFRYIYLIQEFVKKPDRD
HHHCCCCEEEEECCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
IRIFTIGDEAPVGIYRVNSRNWKTNTALGAKAEPLKIDEELQDLALKVKDIIGGFFLGID
EEEEEECCCCCEEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHEEEE
VFEDPERGYIINEVNGVPEYKNTVRVNNFNVSEYLIRKIEEWIKK
EEECCCCCEEEECCCCCCCCCCEEEEECCCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MILGVIYDLLRWEEKNLIQEARKLGHTVIPIYTKDFYYFYNNDSNETLGDLDVVIQRNTS
CHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEEEEEEEECCCCCCCCCEEEEEECCCC
HARAVITSTIFENLSYKTINDSSTLIKCENKLYTLSLLSKHGIRVPKTIVAFSKEKALEL
HHHHHHHHHHHHCCCCEEECCCCCEEEECCCEEEEEEHHHCCCCCCHHHHHHHHHHHHHH
ANKLSYPVVIKPVEGSWGRMVARAIDEDTLRNFLEYQEYTTLQFRYIYLIQEFVKKPDRD
HHHCCCCEEEEECCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
IRIFTIGDEAPVGIYRVNSRNWKTNTALGAKAEPLKIDEELQDLALKVKDIIGGFFLGID
EEEEEECCCCCEEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHEEEE
VFEDPERGYIINEVNGVPEYKNTVRVNNFNVSEYLIRKIEEWIKK
EEECCCCCEEEECCCCCCCCCCEEEEECCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Glu; Ribosomal protein S6 [C]

Specific reaction: Catalyzes the addition of glutamic acids to the carboxyl end of ribosomal protein S6 [C]

General reaction: Ligases; Forming Carbon-Nitrogen Bonds; Acid-D-Amino-Acid Ligases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11572479