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The map label for this gene is alaXM [H]

Identifier: 15920346

GI number: 15920346

Start: 193704

End: 194327

Strand: Reverse

Name: alaXM [H]

Synonym: ST0167

Alternate gene names: 15920346

Gene position: 194327-193704 (Counterclockwise)

Preceding gene: 15920349

Following gene: 15920342

Centisome position: 7.21

GC content: 32.05

Gene sequence:

>624_bases
GTGTTCTTAGACAAAACGGCATTTTATCCTGGAGGAGGAGGTGTAGAAAATGATAAGGGTTACTTTATCTTTAACGGAAA
TAAAATAGAAGTTAATAACGTAAAAGAAATAGATGGAGAAATAGCACATGAAGCTACCTCGGTACCATTTAAGGAAGGAG
ATGAAATCACTGGAGTAATAGATTGGGAAAGAAGATATAGAATGATGAGATTACATACAGCTTCCCATATAATCGCTGCA
ATAGCATATTCAAAGTATAATTCTAAAATAACTGGCGGAAACATTTCACCAGAATATGCTAAAGATGATTTCGATATTGA
TGATAAGAATAAACTATTAGAAATAGTAAAGGAAGCCAACGAAATAGCTAAAAAAGGAATACCAGTAAAAATTTACTTCT
TAAAGAAGGAAGAAGCACTCAAAATACCAGGAATTGTAAAGTTAGCTGAAAGAATGCCACCAAATATAGACATATGGAGA
ATTGTGGAAATTGAAGGAATAGATATTCAAGCTGATGGAGGACCTCACGTTTCTAATACTTTAGAAATAGGAGAAATAGT
ACCTTTAAAAATTGAGAATAGAGGAAAAGGCAAAAAAAGAATATATTATACTGTAAAACCCTAA

Upstream 100 bases:

>100_bases
AACGTAAGTAGTAATTTAAGTTATAGTAGAGAAACTTTACCTAACAGACTGTTATATAAAGGAATTTAAGGCAAAAATCA
CAAAAATTGACGGAAATCTT

Downstream 100 bases:

>100_bases
AATATTCAAATCTCCCAGACATACCTTATACTAAGAATAAACCTTACTCCAAAACCAGCAACTATACCTAGAAAATTGAT
TATAGCTAAATATAAAGAGG

Product: alanyl-tRNA synthetase

Products: AMP; diphosphate; L-alanyl-tRNA(Ala)

Alternate protein names: AlaX-M; Alanyl-tRNA deacylase AlaX-M [H]

Number of amino acids: Translated: 207; Mature: 207

Protein sequence:

>207_residues
MFLDKTAFYPGGGGVENDKGYFIFNGNKIEVNNVKEIDGEIAHEATSVPFKEGDEITGVIDWERRYRMMRLHTASHIIAA
IAYSKYNSKITGGNISPEYAKDDFDIDDKNKLLEIVKEANEIAKKGIPVKIYFLKKEEALKIPGIVKLAERMPPNIDIWR
IVEIEGIDIQADGGPHVSNTLEIGEIVPLKIENRGKGKKRIYYTVKP

Sequences:

>Translated_207_residues
MFLDKTAFYPGGGGVENDKGYFIFNGNKIEVNNVKEIDGEIAHEATSVPFKEGDEITGVIDWERRYRMMRLHTASHIIAA
IAYSKYNSKITGGNISPEYAKDDFDIDDKNKLLEIVKEANEIAKKGIPVKIYFLKKEEALKIPGIVKLAERMPPNIDIWR
IVEIEGIDIQADGGPHVSNTLEIGEIVPLKIENRGKGKKRIYYTVKP
>Mature_207_residues
MFLDKTAFYPGGGGVENDKGYFIFNGNKIEVNNVKEIDGEIAHEATSVPFKEGDEITGVIDWERRYRMMRLHTASHIIAA
IAYSKYNSKITGGNISPEYAKDDFDIDDKNKLLEIVKEANEIAKKGIPVKIYFLKKEEALKIPGIVKLAERMPPNIDIWR
IVEIEGIDIQADGGPHVSNTLEIGEIVPLKIENRGKGKKRIYYTVKP

Specific function: Functions in trans to edit the amino acid moiety from incorrectly charged Ser-tRNA(Ala) [H]

COG id: COG2872

COG function: function code R; Predicted metal-dependent hydrolases related to alanyl-tRNA synthetase HxxxH domain

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. Editing domain AlaX-M subfamily [H]

Homologues:

Organism=Homo sapiens, GI13376886, Length=203, Percent_Identity=29.064039408867, Blast_Score=77, Evalue=1e-14,
Organism=Homo sapiens, GI217416402, Length=203, Percent_Identity=29.064039408867, Blast_Score=77, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018165
- InterPro:   IPR018164
- InterPro:   IPR018163
- InterPro:   IPR012947 [H]

Pfam domain/function: PF01411 tRNA-synt_2c; PF07973 tRNA_SAD [H]

EC number: 6.1.1.7

Molecular weight: Translated: 23322; Mature: 23322

Theoretical pI: Translated: 6.54; Mature: 6.54

Prosite motif: PS50860 AA_TRNA_LIGASE_II_ALA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFLDKTAFYPGGGGVENDKGYFIFNGNKIEVNNVKEIDGEIAHEATSVPFKEGDEITGVI
CCCCCCEECCCCCCCCCCCCEEEEECCEEEECCCHHCCCHHHHHHCCCCCCCCCEEEEEE
DWERRYRMMRLHTASHIIAAIAYSKYNSKITGGNISPEYAKDDFDIDDKNKLLEIVKEAN
EHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHH
EIAKKGIPVKIYFLKKEEALKIPGIVKLAERMPPNIDIWRIVEIEGIDIQADGGPHVSNT
HHHHCCCCEEEEEEECCCCCCCCCHHHHHHHCCCCCCEEEEEEEECEEEECCCCCCCCCE
LEIGEIVPLKIENRGKGKKRIYYTVKP
EECCCEEEEEECCCCCCCEEEEEEECC
>Mature Secondary Structure
MFLDKTAFYPGGGGVENDKGYFIFNGNKIEVNNVKEIDGEIAHEATSVPFKEGDEITGVI
CCCCCCEECCCCCCCCCCCCEEEEECCEEEECCCHHCCCHHHHHHCCCCCCCCCEEEEEE
DWERRYRMMRLHTASHIIAAIAYSKYNSKITGGNISPEYAKDDFDIDDKNKLLEIVKEAN
EHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHH
EIAKKGIPVKIYFLKKEEALKIPGIVKLAERMPPNIDIWRIVEIEGIDIQADGGPHVSNT
HHHHCCCCEEEEEEECCCCCCCCCHHHHHHHCCCCCCEEEEEEEECEEEECCCCCCCCCE
LEIGEIVPLKIENRGKGKKRIYYTVKP
EECCCEEEEEECCCCCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-alanine; tRNA(Ala)

Specific reaction: ATP + L-alanine + tRNA(Ala) = AMP + diphosphate + L-alanyl-tRNA(Ala)

General reaction: Aminoacylation; Esterification [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11427726 [H]