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The map label for this gene is aes [H]

Identifier: 15920250

GI number: 15920250

Start: 94092

End: 95003

Strand: Reverse

Name: aes [H]

Synonym: ST0071

Alternate gene names: 15920250

Gene position: 95003-94092 (Counterclockwise)

Preceding gene: 15920252

Following gene: 15920247

Centisome position: 3.53

GC content: 34.1

Gene sequence:

>912_bases
ATGATAGACCCTAAAATTAAAAAATTATTAGAATCAACAATCCAATTACCTATAGGTAAGGCATCTGTAGAGGAGATAAG
ATCGCTTTTTAAACAATTTTCATCTCTTACACCAAGAGAAGAAGTAGGGAAAATAGAAGACATAACCATTCCGGGTAGTG
AAACAAATATAAAAGCTAGAGTTTATTATCCTAAAACACAAGGTCCTTATGGAGTTTTAGTTTATTATCATGGCGGAGGA
TTTGTTCTCGGCGACATCGAAAGTTATGATCCACTATGTAGGGCTATAACTAACTCTTGTCAATGTGTTACAATTTCCGT
AGATTATAGACTGGCTCCAGAAAACAAATTTCCAGCTGCAGTAGTCGATTCGTTTGACGCATTAAAGTGGGTATACAATA
ATTCAGAGAAATTTAATGGAAAATATGGAATAGCTGTTGGTGGTGATAGTGCTGGTGGCAATTTAGCAGCTGTAACTGCT
ATCTTATCAAAGAAAGAAAATATTAAACTAAAGTACCAAGTTCTTATTTATCCAGCAGTAAGCTTTGATCTAATAACAAA
ATCCCTTTACGATAATGGAGAGGGGTTCTTCCTAACCAGGGAACATATAGACTGGTTCGGTCAACAATATTTACGATCTT
TTGCTGATTTATTGGATTTCAGATTTTCTCCAATATTAGCTGATTTAAATGATTTACCACCAGCATTAATAATAACAGCT
GAACATGATCCATTAAGAGACCAAGGAGAGGCTTATGCTAACAAACTTTTACAATCTGGTGTACAAGTTACGAGTGTTAG
GTTTAATAATGTAATTCATGGTTTCGTTTCGTTCTTTCCCTTTATAGAACAAGGTAGGGACGCTATAGGATTAATAGGAT
ATGTATTAAGAAAAGTATTTTACGGAAAATAA

Upstream 100 bases:

>100_bases
ACTTTAGTCGACTTTGAAAAAAGTTCATTTTTATTGTATAAACGGTAACAACCAGTTATACGTCGTAAAATATTTAATCT
AATTCGAAAAGTAGTAAATA

Downstream 100 bases:

>100_bases
TTTAGAATTTTAATCCCCCTTTAAGGCTTTCGCCAGAATCAATTACAAAAACTTGACCGGTAAGCGATTCTATTTTTAAT
ATCGCAGTAGCAAGTTCCGC

Product: esterase

Products: alcohol; a carboxylate [C]

Alternate protein names: NA

Number of amino acids: Translated: 303; Mature: 303

Protein sequence:

>303_residues
MIDPKIKKLLESTIQLPIGKASVEEIRSLFKQFSSLTPREEVGKIEDITIPGSETNIKARVYYPKTQGPYGVLVYYHGGG
FVLGDIESYDPLCRAITNSCQCVTISVDYRLAPENKFPAAVVDSFDALKWVYNNSEKFNGKYGIAVGGDSAGGNLAAVTA
ILSKKENIKLKYQVLIYPAVSFDLITKSLYDNGEGFFLTREHIDWFGQQYLRSFADLLDFRFSPILADLNDLPPALIITA
EHDPLRDQGEAYANKLLQSGVQVTSVRFNNVIHGFVSFFPFIEQGRDAIGLIGYVLRKVFYGK

Sequences:

>Translated_303_residues
MIDPKIKKLLESTIQLPIGKASVEEIRSLFKQFSSLTPREEVGKIEDITIPGSETNIKARVYYPKTQGPYGVLVYYHGGG
FVLGDIESYDPLCRAITNSCQCVTISVDYRLAPENKFPAAVVDSFDALKWVYNNSEKFNGKYGIAVGGDSAGGNLAAVTA
ILSKKENIKLKYQVLIYPAVSFDLITKSLYDNGEGFFLTREHIDWFGQQYLRSFADLLDFRFSPILADLNDLPPALIITA
EHDPLRDQGEAYANKLLQSGVQVTSVRFNNVIHGFVSFFPFIEQGRDAIGLIGYVLRKVFYGK
>Mature_303_residues
MIDPKIKKLLESTIQLPIGKASVEEIRSLFKQFSSLTPREEVGKIEDITIPGSETNIKARVYYPKTQGPYGVLVYYHGGG
FVLGDIESYDPLCRAITNSCQCVTISVDYRLAPENKFPAAVVDSFDALKWVYNNSEKFNGKYGIAVGGDSAGGNLAAVTA
ILSKKENIKLKYQVLIYPAVSFDLITKSLYDNGEGFFLTREHIDWFGQQYLRSFADLLDFRFSPILADLNDLPPALIITA
EHDPLRDQGEAYANKLLQSGVQVTSVRFNNVIHGFVSFFPFIEQGRDAIGLIGYVLRKVFYGK

Specific function: Displays esterase activity towards short chain fatty esters (acyl chain length of up to 8 carbons). Able to hydrolyze triacetylglycerol (triacetin) and tributyrylglycerol (tributyrin), but not trioleylglycerol (triolein) or cholesterol oleate. Negatively

COG id: COG0657

COG function: function code I; Esterase/lipase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'GDXG' lipolytic enzyme family [H]

Homologues:

Organism=Homo sapiens, GI68299767, Length=313, Percent_Identity=32.9073482428115, Blast_Score=119, Evalue=5e-27,
Organism=Homo sapiens, GI206597554, Length=307, Percent_Identity=31.9218241042345, Blast_Score=114, Evalue=2e-25,
Organism=Homo sapiens, GI68051721, Length=296, Percent_Identity=30.4054054054054, Blast_Score=100, Evalue=3e-21,
Organism=Homo sapiens, GI226423947, Length=304, Percent_Identity=29.6052631578947, Blast_Score=93, Evalue=3e-19,
Organism=Homo sapiens, GI21328446, Length=111, Percent_Identity=34.2342342342342, Blast_Score=65, Evalue=7e-11,
Organism=Escherichia coli, GI1786682, Length=250, Percent_Identity=27.2, Blast_Score=97, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI17567059, Length=267, Percent_Identity=29.9625468164794, Blast_Score=105, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI72001146, Length=147, Percent_Identity=36.0544217687075, Blast_Score=83, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI71996133, Length=136, Percent_Identity=37.5, Blast_Score=80, Evalue=9e-16,
Organism=Caenorhabditis elegans, GI17540028, Length=139, Percent_Identity=36.6906474820144, Blast_Score=78, Evalue=5e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013094
- InterPro:   IPR002168 [H]

Pfam domain/function: PF07859 Abhydrolase_3 [H]

EC number: 3.1.1.- [C]

Molecular weight: Translated: 33806; Mature: 33806

Theoretical pI: Translated: 6.53; Mature: 6.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
0.3 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
0.3 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIDPKIKKLLESTIQLPIGKASVEEIRSLFKQFSSLTPREEVGKIEDITIPGSETNIKAR
CCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCHHHCCCEEEEEECCCCCCEEEE
VYYPKTQGPYGVLVYYHGGGFVLGDIESYDPLCRAITNSCQCVTISVDYRLAPENKFPAA
EEECCCCCCEEEEEEEECCEEEEECCCCCCHHHHHHCCCCEEEEEEEEEEECCCCCCCHH
VVDSFDALKWVYNNSEKFNGKYGIAVGGDSAGGNLAAVTAILSKKENIKLKYQVLIYPAV
HHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHCCCCCEEEEEEEEEECH
SFDLITKSLYDNGEGFFLTREHIDWFGQQYLRSFADLLDFRFSPILADLNDLPPALIITA
HHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCEEEEEE
EHDPLRDQGEAYANKLLQSGVQVTSVRFNNVIHGFVSFFPFIEQGRDAIGLIGYVLRKVF
CCCCCCHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
YGK
CCC
>Mature Secondary Structure
MIDPKIKKLLESTIQLPIGKASVEEIRSLFKQFSSLTPREEVGKIEDITIPGSETNIKAR
CCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCHHHCCCEEEEEECCCCCCEEEE
VYYPKTQGPYGVLVYYHGGGFVLGDIESYDPLCRAITNSCQCVTISVDYRLAPENKFPAA
EEECCCCCCEEEEEEEECCEEEEECCCCCCHHHHHHCCCCEEEEEEEEEEECCCCCCCHH
VVDSFDALKWVYNNSEKFNGKYGIAVGGDSAGGNLAAVTAILSKKENIKLKYQVLIYPAV
HHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHCCCCCEEEEEEEEEECH
SFDLITKSLYDNGEGFFLTREHIDWFGQQYLRSFADLLDFRFSPILADLNDLPPALIITA
HHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCEEEEEE
EHDPLRDQGEAYANKLLQSGVQVTSVRFNNVIHGFVSFFPFIEQGRDAIGLIGYVLRKVF
CCCCCCHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
YGK
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Carboxylic ester; H2O [C]

Specific reaction: Carboxylic ester + H2O = an alcohol + carboxylate [C]

General reaction: Hydrolase; Acting on ester bonds; Carboxylic ester hydrolases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA