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The map label for this gene is abfD [H]

Identifier: 15920242

GI number: 15920242

Start: 82984

End: 84396

Strand: Reverse

Name: abfD [H]

Synonym: ST0063

Alternate gene names: 15920242

Gene position: 84396-82984 (Counterclockwise)

Preceding gene: 15920244

Following gene: 15920241

Centisome position: 3.13

GC content: 35.24

Gene sequence:

>1413_bases
ATGAAATTATCAAATATGAGAAGTAAGGAAGATTACTTAAAGTCGTTAAACGATGGAAGAAGAGTAATGTATCGAGGCAA
ATACATATCAAATATTGTTGAACATCCCGTGTTAAAAATATCTGCACTTCACGCGTCAAAATTATTTGAATATCCAGATA
GATCTTACGACGACCAACAGTACGGTAAAATAAGTAAATACTTCAAGGTACCAAAAACATCCCAAGACTTACTAGATAGG
CATAAACTAGTATACGATACAACTATTTTCTGCAATGGTATATTTAACATTTCACAAGCCATCGGTAGTGATGCTCTCTT
TTCCTTAATGATAACAGCTAAAAAATTAGATAAAAAGTACGGTAAAGACTATTTCAGCAGAGTTAAGAAATACTACGAGT
ATGTCGTAAAAAATGATCTAACCTTAGCAACAGCACAGACTGATGTTAAGGGTGATAGGTCAAAAAGACCATCAAAACAG
CCAGACCCAGATTTATATGTTAGAGTAGTTGATGTAAGAAATGATGGCATCGTAGTTAGAGGGGCAAAAGCTCACACTAC
TCAAGCAGCTGTAGCTGATGAGATAATAGTTATACCAACAAGGGCTATGGGAGAAGAGGATAAGGATTACGCTATAGCCT
TTGCAGTTCCTGCTAATACACCGGGTTTGAAGATGATTGTAAGACCAATAGATGAGATTGAGGGAAATTCCTCATCAGTC
TTAAGTGTAAAAGATTATGAGTTAGAAACACTGACAATCTTCGATAACGTCTTTGTACCATGGGATAGAGTATTCCTATT
TAGGGAATATGAATTTGCTGGAACCCTAGCTAGACTATTTGCAACATATCATAGGTTTACAGCAATTTCTTATAGAGCTG
CTATGGCAAATCTCTATTTAGGTTCAGCAATACTTTCAGCAGAGGCAAACGGAATATCAAACGAGAAGCACGTTAGAGAC
GATATAATAGACATAATACTTTATAAGGAAATACTAAGGATGTCGGCAATAGCCTCGTCTGTTTACCCAGAAATTGATGA
AGAAATTGCAATCCCTAACTATATCTTTACTAATATAGGTAAGCTCTACACTAACACACACTTCCATGAAGTTGTTAAGG
ATTTAATTGATATAGCTGGCGGTATAATATCAACATTACCCTCAGAAGAAGATCTTAAGAGTGACGAAGGAGAGGTTATA
AGTAAGTATTTGAGAGGTGCGATAGACGGAAAAGAGAGGATCGATATTTTAAAATTTGTGAAGGAAATAGATGGAAGTAG
TTCTTTAGTAGGGTATTGGCTTACAACTATGATCCATGCTGAAGGTTCAATTGAGGCAAGTAAAATCGAACTATTCAGAA
ATTACGATTTTAATGAGAGTAAGGAACTTGTAAAGAAAATAATTTCAAGTTAA

Upstream 100 bases:

>100_bases
ATAATAGCATAATGCTCTATAAATTCGTTAAGCTAAAGATATGTACAAATTGAGGTAAGGTATCCTACTCTTAGTACTAT
GTAGATTTATATAACTCCCC

Downstream 100 bases:

>100_bases
ATGTGATTTTTTCTCCGCTATATTTAAATTCGGAAAAGAAGTAAATTATAATATGTACGAATTTCCATTAACAATCCAAC
ACATTTTTTGGAGAGTTGAA

Product: hypothetical protein

Products: NA

Alternate protein names: 4-BUDH; Gamma-aminobutyrate metabolism dehydratase/isomerase [H]

Number of amino acids: Translated: 470; Mature: 470

Protein sequence:

>470_residues
MKLSNMRSKEDYLKSLNDGRRVMYRGKYISNIVEHPVLKISALHASKLFEYPDRSYDDQQYGKISKYFKVPKTSQDLLDR
HKLVYDTTIFCNGIFNISQAIGSDALFSLMITAKKLDKKYGKDYFSRVKKYYEYVVKNDLTLATAQTDVKGDRSKRPSKQ
PDPDLYVRVVDVRNDGIVVRGAKAHTTQAAVADEIIVIPTRAMGEEDKDYAIAFAVPANTPGLKMIVRPIDEIEGNSSSV
LSVKDYELETLTIFDNVFVPWDRVFLFREYEFAGTLARLFATYHRFTAISYRAAMANLYLGSAILSAEANGISNEKHVRD
DIIDIILYKEILRMSAIASSVYPEIDEEIAIPNYIFTNIGKLYTNTHFHEVVKDLIDIAGGIISTLPSEEDLKSDEGEVI
SKYLRGAIDGKERIDILKFVKEIDGSSSLVGYWLTTMIHAEGSIEASKIELFRNYDFNESKELVKKIISS

Sequences:

>Translated_470_residues
MKLSNMRSKEDYLKSLNDGRRVMYRGKYISNIVEHPVLKISALHASKLFEYPDRSYDDQQYGKISKYFKVPKTSQDLLDR
HKLVYDTTIFCNGIFNISQAIGSDALFSLMITAKKLDKKYGKDYFSRVKKYYEYVVKNDLTLATAQTDVKGDRSKRPSKQ
PDPDLYVRVVDVRNDGIVVRGAKAHTTQAAVADEIIVIPTRAMGEEDKDYAIAFAVPANTPGLKMIVRPIDEIEGNSSSV
LSVKDYELETLTIFDNVFVPWDRVFLFREYEFAGTLARLFATYHRFTAISYRAAMANLYLGSAILSAEANGISNEKHVRD
DIIDIILYKEILRMSAIASSVYPEIDEEIAIPNYIFTNIGKLYTNTHFHEVVKDLIDIAGGIISTLPSEEDLKSDEGEVI
SKYLRGAIDGKERIDILKFVKEIDGSSSLVGYWLTTMIHAEGSIEASKIELFRNYDFNESKELVKKIISS
>Mature_470_residues
MKLSNMRSKEDYLKSLNDGRRVMYRGKYISNIVEHPVLKISALHASKLFEYPDRSYDDQQYGKISKYFKVPKTSQDLLDR
HKLVYDTTIFCNGIFNISQAIGSDALFSLMITAKKLDKKYGKDYFSRVKKYYEYVVKNDLTLATAQTDVKGDRSKRPSKQ
PDPDLYVRVVDVRNDGIVVRGAKAHTTQAAVADEIIVIPTRAMGEEDKDYAIAFAVPANTPGLKMIVRPIDEIEGNSSSV
LSVKDYELETLTIFDNVFVPWDRVFLFREYEFAGTLARLFATYHRFTAISYRAAMANLYLGSAILSAEANGISNEKHVRD
DIIDIILYKEILRMSAIASSVYPEIDEEIAIPNYIFTNIGKLYTNTHFHEVVKDLIDIAGGIISTLPSEEDLKSDEGEVI
SKYLRGAIDGKERIDILKFVKEIDGSSSLVGYWLTTMIHAEGSIEASKIELFRNYDFNESKELVKKIISS

Specific function: Catalyzes the reversible conversion of 4-hydroxybutyryl- CoA to crotonyl-CoA. The mechanism of the reaction seems to go through three steps:(1) the FAD-dependent oxidation of 4- hydroxybutyryl-CoA to 4-hydroxycrotonyl-CoA; (2) the hydroxyl group is substi

COG id: COG2368

COG function: function code Q; Aromatic ring hydroxylase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006091
- InterPro:   IPR009075
- InterPro:   IPR009100
- InterPro:   IPR004925 [H]

Pfam domain/function: PF03241 HpaB [H]

EC number: =4.2.1.120; =5.3.3.3 [H]

Molecular weight: Translated: 53313; Mature: 53313

Theoretical pI: Translated: 6.45; Mature: 6.45

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLSNMRSKEDYLKSLNDGRRVMYRGKYISNIVEHPVLKISALHASKLFEYPDRSYDDQQ
CCCCCCCCHHHHHHHCCCCCEEEECCHHHHHHHHCCHHEEHHHHHHHHHCCCCCCCCCHH
YGKISKYFKVPKTSQDLLDRHKLVYDTTIFCNGIFNISQAIGSDALFSLMITAKKLDKKY
HHHHHHHHCCCCCHHHHHHHHHHHEEHHHEECHHHHHHHHHCCHHHHHHHHHHHHHHHHH
GKDYFSRVKKYYEYVVKNDLTLATAQTDVKGDRSKRPSKQPDPDLYVRVVDVRNDGIVVR
CHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCCCCEEEEEEEECCCCEEEE
GAKAHTTQAAVADEIIVIPTRAMGEEDKDYAIAFAVPANTPGLKMIVRPIDEIEGNSSSV
CCCCCCHHHHHHCCEEEECCHHCCCCCCCEEEEEEECCCCCCHHHHEECHHHCCCCCCCE
LSVKDYELETLTIFDNVFVPWDRVFLFREYEFAGTLARLFATYHRFTAISYRAAMANLYL
EEEECCCEEEEEEECCCCCCHHHEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GSAILSAEANGISNEKHVRDDIIDIILYKEILRMSAIASSVYPEIDEEIAIPNYIFTNIG
HHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHCCCHHHHHHHH
KLYTNTHFHEVVKDLIDIAGGIISTLPSEEDLKSDEGEVISKYLRGAIDGKERIDILKFV
HHHCCHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHHH
KEIDGSSSLVGYWLTTMIHAEGSIEASKIELFRNYDFNESKELVKKIISS
HHCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCHHHHHHHHHHCC
>Mature Secondary Structure
MKLSNMRSKEDYLKSLNDGRRVMYRGKYISNIVEHPVLKISALHASKLFEYPDRSYDDQQ
CCCCCCCCHHHHHHHCCCCCEEEECCHHHHHHHHCCHHEEHHHHHHHHHCCCCCCCCCHH
YGKISKYFKVPKTSQDLLDRHKLVYDTTIFCNGIFNISQAIGSDALFSLMITAKKLDKKY
HHHHHHHHCCCCCHHHHHHHHHHHEEHHHEECHHHHHHHHHCCHHHHHHHHHHHHHHHHH
GKDYFSRVKKYYEYVVKNDLTLATAQTDVKGDRSKRPSKQPDPDLYVRVVDVRNDGIVVR
CHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCCCCEEEEEEEECCCCEEEE
GAKAHTTQAAVADEIIVIPTRAMGEEDKDYAIAFAVPANTPGLKMIVRPIDEIEGNSSSV
CCCCCCHHHHHHCCEEEECCHHCCCCCCCEEEEEEECCCCCCHHHHEECHHHCCCCCCCE
LSVKDYELETLTIFDNVFVPWDRVFLFREYEFAGTLARLFATYHRFTAISYRAAMANLYL
EEEECCCEEEEEEECCCCCCHHHEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GSAILSAEANGISNEKHVRDDIIDIILYKEILRMSAIASSVYPEIDEEIAIPNYIFTNIG
HHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHCCCHHHHHHHH
KLYTNTHFHEVVKDLIDIAGGIISTLPSEEDLKSDEGEVISKYLRGAIDGKERIDILKFV
HHHCCHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHHH
KEIDGSSSLVGYWLTTMIHAEGSIEASKIELFRNYDFNESKELVKKIISS
HHCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11041350; 8344309 [H]