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The map label for this gene is benM [H]

Identifier: 16119902

GI number: 16119902

Start: 105676

End: 106623

Strand: Direct

Name: benM [H]

Synonym: Atu6088

Alternate gene names: 16119902

Gene position: 105676-106623 (Clockwise)

Preceding gene: 159161971

Following gene: 16119903

Centisome position: 49.33

GC content: 59.28

Gene sequence:

>948_bases
ATGAACCTCGACGACATCGAAGCTTTCATCGCAGTGGTCGGAGCCAGGTCGCTCAGCCAAGCGGCGGCAATTCTTTCGTT
GACCCAGTCGGCAATCTCGAAGCGCATCCAGAACCTCGAGAAGGACCTCGGTGTTACCCTGCTCGACAGAAGCGTCAAGC
CACCCGTTCCAACCACCGTAGGTCTCGGCGTCCACGAGCAGTGCCTCAATATTCTCAGGGAGGTCGACAAGCTCCGCTTG
ACGTTGAGTGCCCGCTCGAACCTAACGGGTGGTGCGCGCTTGGGACTGACCCAGGCAGTCTGCGATGTGATTCTTGCCGA
CATAGTCCATCTGCAACAGGAGCGATGGCCGGAACTGTCTATACGTGCCACCACAGGCTGGGCGAACCAGATTGTTGAAA
AGCTCGTCGACGGCCAGATCGATGCCGCGATCGTATTGATGCCCACACGGAAAATCTTTCCGCACAAAGTGACCGCCGTT
TCGCTGGCGCAGGTCGAGATGGCGATCGTGGGGCCGCTCGGATCGGGCGGAGACCGTGCCCACCGCCTTGTCACCCTTCA
CAAATCCGGATGGATTCTGAACCCCGACGGATGCGGTTTCAGGGCAGAACTCCAGCGCGTGCATGCGGTGCTCAATCTTC
CGTTCAAGGTCAATCTCGACACCTTTGCTCGAGAGACGCAGTTGGAGATGGTTGCCGCAGGTCTCGGACTTGGACTCGTT
CCCGTTCCCCTTCTGGAGATGAGCCCCCACCGTACCAAACTGGAGATCATCACAGTCAGCGACTTTTCGTTGCGCGCAGA
ACTCTGGCTCTGTCACGCGCCGACTCTCGGTGCTCTCGAACCACCCATCAGCGCATTTGGAGCGAGAGCCGCAACGCTGT
TTCAGGGCGCGGCAGCTCCCAGCAGGCTGGTCGACTTTGAAGCTGCCAGCCAGACCATCACCTCGTGA

Upstream 100 bases:

>100_bases
GTCTTCGTGTGCGCAGTTCGCAGGCGTTCACGTCAATCGAGGAGAAGGTTCTTTCCAGGGTACTTAACTCCAGAAATGCA
CCAAGCGGAGACGACTGCAA

Downstream 100 bases:

>100_bases
ATAGCACACGAGATCGATAGCAGGAGAAAATATGTCAGCCCCGATAACGAAGCACAGCCTCAGGTACGACGAAGTGCGGC
AGCATCTTCTGGCCCGCTCG

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: Ben and cat operon transcriptional regulator [H]

Number of amino acids: Translated: 315; Mature: 315

Protein sequence:

>315_residues
MNLDDIEAFIAVVGARSLSQAAAILSLTQSAISKRIQNLEKDLGVTLLDRSVKPPVPTTVGLGVHEQCLNILREVDKLRL
TLSARSNLTGGARLGLTQAVCDVILADIVHLQQERWPELSIRATTGWANQIVEKLVDGQIDAAIVLMPTRKIFPHKVTAV
SLAQVEMAIVGPLGSGGDRAHRLVTLHKSGWILNPDGCGFRAELQRVHAVLNLPFKVNLDTFARETQLEMVAAGLGLGLV
PVPLLEMSPHRTKLEIITVSDFSLRAELWLCHAPTLGALEPPISAFGARAATLFQGAAAPSRLVDFEAASQTITS

Sequences:

>Translated_315_residues
MNLDDIEAFIAVVGARSLSQAAAILSLTQSAISKRIQNLEKDLGVTLLDRSVKPPVPTTVGLGVHEQCLNILREVDKLRL
TLSARSNLTGGARLGLTQAVCDVILADIVHLQQERWPELSIRATTGWANQIVEKLVDGQIDAAIVLMPTRKIFPHKVTAV
SLAQVEMAIVGPLGSGGDRAHRLVTLHKSGWILNPDGCGFRAELQRVHAVLNLPFKVNLDTFARETQLEMVAAGLGLGLV
PVPLLEMSPHRTKLEIITVSDFSLRAELWLCHAPTLGALEPPISAFGARAATLFQGAAAPSRLVDFEAASQTITS
>Mature_315_residues
MNLDDIEAFIAVVGARSLSQAAAILSLTQSAISKRIQNLEKDLGVTLLDRSVKPPVPTTVGLGVHEQCLNILREVDKLRL
TLSARSNLTGGARLGLTQAVCDVILADIVHLQQERWPELSIRATTGWANQIVEKLVDGQIDAAIVLMPTRKIFPHKVTAV
SLAQVEMAIVGPLGSGGDRAHRLVTLHKSGWILNPDGCGFRAELQRVHAVLNLPFKVNLDTFARETQLEMVAAGLGLGLV
PVPLLEMSPHRTKLEIITVSDFSLRAELWLCHAPTLGALEPPISAFGARAATLFQGAAAPSRLVDFEAASQTITS

Specific function: Positive regulator of the ben and cat genes for benzoate degradation. BenM is necessary for ben gene expression but not for expression of the cat genes, which can be regulated by CatM. Binds to the inducers cis,cis-muconate and benzoate [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790399, Length=146, Percent_Identity=28.0821917808219, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1790208, Length=297, Percent_Identity=26.9360269360269, Blast_Score=66, Evalue=4e-12,
Organism=Escherichia coli, GI1787806, Length=287, Percent_Identity=24.390243902439, Blast_Score=65, Evalue=6e-12,
Organism=Escherichia coli, GI1788481, Length=282, Percent_Identity=25.177304964539, Blast_Score=64, Evalue=9e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33850; Mature: 33850

Theoretical pI: Translated: 7.24; Mature: 7.24

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLDDIEAFIAVVGARSLSQAAAILSLTQSAISKRIQNLEKDLGVTLLDRSVKPPVPTTV
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCCCCEE
GLGVHEQCLNILREVDKLRLTLSARSNLTGGARLGLTQAVCDVILADIVHLQQERWPELS
CCCHHHHHHHHHHHHHHHEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEE
IRATTGWANQIVEKLVDGQIDAAIVLMPTRKIFPHKVTAVSLAQVEMAIVGPLGSGGDRA
EEEECCHHHHHHHHHHCCCCCEEEEEEECCCCCCCHHHHHHHHHEEEEEEECCCCCCCCC
HRLVTLHKSGWILNPDGCGFRAELQRVHAVLNLPFKVNLDTFARETQLEMVAAGLGLGLV
EEEEEEECCCCEECCCCCCHHHHHHHHHHHHCCCEEECHHHHHHHHHHHHHHHHCCCCCC
PVPLLEMSPHRTKLEIITVSDFSLRAELWLCHAPTLGALEPPISAFGARAATLFQGAAAP
CCCCCCCCCCCEEEEEEEECCCEEEEEEEEEECCCCCCCCCCHHHHCCHHHHHHHCCCCC
SRLVDFEAASQTITS
HHHHHHHHHHHHCCC
>Mature Secondary Structure
MNLDDIEAFIAVVGARSLSQAAAILSLTQSAISKRIQNLEKDLGVTLLDRSVKPPVPTTV
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCCCCEE
GLGVHEQCLNILREVDKLRLTLSARSNLTGGARLGLTQAVCDVILADIVHLQQERWPELS
CCCHHHHHHHHHHHHHHHEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEE
IRATTGWANQIVEKLVDGQIDAAIVLMPTRKIFPHKVTAVSLAQVEMAIVGPLGSGGDRA
EEEECCHHHHHHHHHHCCCCCEEEEEEECCCCCCCHHHHHHHHHEEEEEEECCCCCCCCC
HRLVTLHKSGWILNPDGCGFRAELQRVHAVLNLPFKVNLDTFARETQLEMVAAGLGLGLV
EEEEEEECCCCEECCCCCCHHHHHHHHHHHHCCCEEECHHHHHHHHHHHHHHHHCCCCCC
PVPLLEMSPHRTKLEIITVSDFSLRAELWLCHAPTLGALEPPISAFGARAATLFQGAAAP
CCCCCCCCCCCEEEEEEEECCCEEEEEEEEEECCCCCCCCCCHHHHCCHHHHHHHCCCCC
SRLVDFEAASQTITS
HHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9573203 [H]