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The map label for this gene is phaD [H]

Identifier: 159184349

GI number: 159184349

Start: 498246

End: 499889

Strand: Reverse

Name: phaD [H]

Synonym: Atu0510

Alternate gene names: 159184349

Gene position: 499889-498246 (Counterclockwise)

Preceding gene: 15887859

Following gene: 159184348

Centisome position: 17.59

GC content: 62.1

Gene sequence:

>1644_bases
ATGTCATTCCCCTTCCACATCGTTGTCGCCCCGATTCTGCTGCCGCTGATCACCGCCGCCCTGCTGCTGTTCTTCGATGA
GCGCCAGCGCGTCGCAAAGGCCGCCATCAGCTTCACCTCCACCGTGCTGTTGCTGATCGTCGCGATCCTGCTGTTCCGCG
AGGTGAATTCGACAGTTAATACCGAGATCGCGGCAGTTGTCTCGAGCGGCGTCTACCTGCTCGGCAACTGGCCGGCTCCT
TTCGGCATCGTGCTCGTAGCAGACCGCCTGTCCGGCCTGATGGTGCTTCTGACCGCGCTGCTCGCCATTCCCTCGCTGAT
CTATTCAATGGCGAAATGGCACAAGTCCGGCGCGCATTTCCATTCGCTGTTCCAGATGATGCTGATGGGCGTCAATGGCG
CCTTCCTGACCGGCGACCTCTTCAATCTCTTCGTTTTCTTCGAAGTGATGCTGGCCGCCTCCTATGGCCTGCTGCTGCAT
GGCTCCGGCCAGCAGCGCGTGAAAGCGGGACTGCATTACATCGCGATCAATCTGGTCGCCGCGCTGTTCTTCCTGATCGG
CGTCAGCCTCATCTATGGCGTGACAGGCACGCTGAACATGGGCGATCTTGCCCACCGCATCGAGGGCCTCAACCCCGACC
AGCGCATGCTGCTGGAAACCGGTGCGGCCATCCTCGGCATCGCATTTCTGGTGAAGGCGGGCATGTGGCCGCTGAATTTC
TGGCTGCCTTCGGCCTATGGCGCGGCTTCGGCCCCGGTGGGCGGTCTGTTCGCGATCATGAGCAAGGTCGGCATCTATGT
CATTGCCCGCCTGTCATTCCTGCTCTTCGGGCAGACGGCCGGTGAATCCGCGGGCTTCGGCCATGATGCCCTGCTGGTCG
GTGGCATCGCCACCATCATCTTCGGCGCCATCGGGGTTCTGGCGTCGCAGGCGCTCGGGCGGCTGGCCGGTTTTTCGGTT
CTGGTGTCATCGGGAACGCTTCTCGCCGCCATGGGCACCGGCAATCCGACGGTCGCGGCCGGTGCGCTCTATTACATGGT
CAGCTCCACCCTCACCATCAGCGCCTTCTTCATGCTGATCGAGCTCGTCGAGCGCGGACAGGATGCGGGCGCAAACGTTC
TGGCCGTCACCATGGAGGCCTATGGCGAGGGCGAGGAAGAAGACGAGGAAGAAGAAGTGGGGGTGACCATGCCCGCCACC
ATTGCGGTGCTCGGCGCCTGTTTTGCCGCCTGCGGCATTCTTCTGGCCGGTCTGCCGCCGCTTTCCGGCTTCATTGCCAA
ATTCTCGATGCTGACCGCCATTCTCAACCCTTCCGGGCTTGGCGCCAATGACAGCGTTTCTACGCTTTCCTGGTGGCTGG
TTTTCCTGATCGTGTTCGCCGGCTTCGCCTCGCTGGTCTCGATGACCCGCGCCGGCATCCGCACCTTCTGGGCCTCGATC
GAAGGAACGGTGCCACGCGTTCTCGTTATCGAAATCGCGCCCATCATGCTGTTGCTCGGACTGACGCTCGCCATGACCAT
ACAAGCGGGACCGGTGATGCGATACATGCAGGAGACGGCGCGTATTCTCGACCTGCCGGCGAGCTATATTCAGGGCGTCA
TTTCCGCGCCGCGAGCGGGCAGCAATCCGGAGGCAGAGCCATGA

Upstream 100 bases:

>100_bases
TCGTCATCGGCTTTGCGACGACGGCGCTGTTCCTCGTTGTGCTGCTTGCTGCCCGCGGGCTCACCGGTACTGACCATGTG
GACGGGAGGGAATCGAAATG

Downstream 100 bases:

>100_bases
TGAGCCGTCTCCTCCCCTACCCGCTCCTGACGGTTTCGTTGATCTTCTTTTGGCTGACGATTAACAGCTTTTCGGCGGGG
CACCTGCTGCTCGGCACCGG

Product: putative monovalent cation/H+ antiporter subunit D

Products: NAD+; ubiquinol [C]

Alternate protein names: pH adaptation potassium efflux system protein D; Pha system subunit D [H]

Number of amino acids: Translated: 547; Mature: 546

Protein sequence:

>547_residues
MSFPFHIVVAPILLPLITAALLLFFDERQRVAKAAISFTSTVLLLIVAILLFREVNSTVNTEIAAVVSSGVYLLGNWPAP
FGIVLVADRLSGLMVLLTALLAIPSLIYSMAKWHKSGAHFHSLFQMMLMGVNGAFLTGDLFNLFVFFEVMLAASYGLLLH
GSGQQRVKAGLHYIAINLVAALFFLIGVSLIYGVTGTLNMGDLAHRIEGLNPDQRMLLETGAAILGIAFLVKAGMWPLNF
WLPSAYGAASAPVGGLFAIMSKVGIYVIARLSFLLFGQTAGESAGFGHDALLVGGIATIIFGAIGVLASQALGRLAGFSV
LVSSGTLLAAMGTGNPTVAAGALYYMVSSTLTISAFFMLIELVERGQDAGANVLAVTMEAYGEGEEEDEEEEVGVTMPAT
IAVLGACFAACGILLAGLPPLSGFIAKFSMLTAILNPSGLGANDSVSTLSWWLVFLIVFAGFASLVSMTRAGIRTFWASI
EGTVPRVLVIEIAPIMLLLGLTLAMTIQAGPVMRYMQETARILDLPASYIQGVISAPRAGSNPEAEP

Sequences:

>Translated_547_residues
MSFPFHIVVAPILLPLITAALLLFFDERQRVAKAAISFTSTVLLLIVAILLFREVNSTVNTEIAAVVSSGVYLLGNWPAP
FGIVLVADRLSGLMVLLTALLAIPSLIYSMAKWHKSGAHFHSLFQMMLMGVNGAFLTGDLFNLFVFFEVMLAASYGLLLH
GSGQQRVKAGLHYIAINLVAALFFLIGVSLIYGVTGTLNMGDLAHRIEGLNPDQRMLLETGAAILGIAFLVKAGMWPLNF
WLPSAYGAASAPVGGLFAIMSKVGIYVIARLSFLLFGQTAGESAGFGHDALLVGGIATIIFGAIGVLASQALGRLAGFSV
LVSSGTLLAAMGTGNPTVAAGALYYMVSSTLTISAFFMLIELVERGQDAGANVLAVTMEAYGEGEEEDEEEEVGVTMPAT
IAVLGACFAACGILLAGLPPLSGFIAKFSMLTAILNPSGLGANDSVSTLSWWLVFLIVFAGFASLVSMTRAGIRTFWASI
EGTVPRVLVIEIAPIMLLLGLTLAMTIQAGPVMRYMQETARILDLPASYIQGVISAPRAGSNPEAEP
>Mature_546_residues
SFPFHIVVAPILLPLITAALLLFFDERQRVAKAAISFTSTVLLLIVAILLFREVNSTVNTEIAAVVSSGVYLLGNWPAPF
GIVLVADRLSGLMVLLTALLAIPSLIYSMAKWHKSGAHFHSLFQMMLMGVNGAFLTGDLFNLFVFFEVMLAASYGLLLHG
SGQQRVKAGLHYIAINLVAALFFLIGVSLIYGVTGTLNMGDLAHRIEGLNPDQRMLLETGAAILGIAFLVKAGMWPLNFW
LPSAYGAASAPVGGLFAIMSKVGIYVIARLSFLLFGQTAGESAGFGHDALLVGGIATIIFGAIGVLASQALGRLAGFSVL
VSSGTLLAAMGTGNPTVAAGALYYMVSSTLTISAFFMLIELVERGQDAGANVLAVTMEAYGEGEEEDEEEEVGVTMPATI
AVLGACFAACGILLAGLPPLSGFIAKFSMLTAILNPSGLGANDSVSTLSWWLVFLIVFAGFASLVSMTRAGIRTFWASIE
GTVPRVLVIEIAPIMLLLGLTLAMTIQAGPVMRYMQETARILDLPASYIQGVISAPRAGSNPEAEP

Specific function: Part of a K(+) efflux system which is required for the adaptation of R.meliloti to alkaline pH as well as for the infection process during symbiotic nodule development [H]

COG id: COG0651

COG function: function code CP; Formate hydrogenlyase subunit 3/Multisubunit Na+/H+ antiporter, MnhD subunit

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CPA3 antiporters (TC 2.A.63) subunit D family [H]

Homologues:

Organism=Escherichia coli, GI1788613, Length=306, Percent_Identity=32.3529411764706, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1788827, Length=384, Percent_Identity=26.3020833333333, Blast_Score=79, Evalue=7e-16,
Organism=Escherichia coli, GI1788829, Length=442, Percent_Identity=24.4343891402715, Blast_Score=73, Evalue=6e-14,
Organism=Escherichia coli, GI145693160, Length=324, Percent_Identity=26.8518518518519, Blast_Score=68, Evalue=2e-12,
Organism=Escherichia coli, GI1788831, Length=476, Percent_Identity=25, Blast_Score=64, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004775
- InterPro:   IPR001750 [H]

Pfam domain/function: PF00361 Oxidored_q1 [H]

EC number: 1.6.99.5 [C]

Molecular weight: Translated: 57593; Mature: 57462

Theoretical pI: Translated: 5.17; Mature: 5.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSFPFHIVVAPILLPLITAALLLFFDERQRVAKAAISFTSTVLLLIVAILLFREVNSTVN
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TEIAAVVSSGVYLLGNWPAPFGIVLVADRLSGLMVLLTALLAIPSLIYSMAKWHKSGAHF
HHHHHHHHCCEEEEECCCCCHHHEEHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCHHH
HSLFQMMLMGVNGAFLTGDLFNLFVFFEVMLAASYGLLLHGSGQQRVKAGLHYIAINLVA
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHHHHH
ALFFLIGVSLIYGVTGTLNMGDLAHRIEGLNPDQRMLLETGAAILGIAFLVKAGMWPLNF
HHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEE
WLPSAYGAASAPVGGLFAIMSKVGIYVIARLSFLLFGQTAGESAGFGHDALLVGGIATII
ECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHH
FGAIGVLASQALGRLAGFSVLVSSGTLLAAMGTGNPTVAAGALYYMVSSTLTISAFFMLI
HHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHH
ELVERGQDAGANVLAVTMEAYGEGEEEDEEEEVGVTMPATIAVLGACFAACGILLAGLPP
HHHHCCCCCCCCEEEEEEHHHCCCCCCCCHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCC
LSGFIAKFSMLTAILNPSGLGANDSVSTLSWWLVFLIVFAGFASLVSMTRAGIRTFWASI
HHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EGTVPRVLVIEIAPIMLLLGLTLAMTIQAGPVMRYMQETARILDLPASYIQGVISAPRAG
CCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCHHHHHHHHCCCCCC
SNPEAEP
CCCCCCC
>Mature Secondary Structure 
SFPFHIVVAPILLPLITAALLLFFDERQRVAKAAISFTSTVLLLIVAILLFREVNSTVN
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TEIAAVVSSGVYLLGNWPAPFGIVLVADRLSGLMVLLTALLAIPSLIYSMAKWHKSGAHF
HHHHHHHHCCEEEEECCCCCHHHEEHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCHHH
HSLFQMMLMGVNGAFLTGDLFNLFVFFEVMLAASYGLLLHGSGQQRVKAGLHYIAINLVA
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHHHHH
ALFFLIGVSLIYGVTGTLNMGDLAHRIEGLNPDQRMLLETGAAILGIAFLVKAGMWPLNF
HHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEE
WLPSAYGAASAPVGGLFAIMSKVGIYVIARLSFLLFGQTAGESAGFGHDALLVGGIATII
ECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHH
FGAIGVLASQALGRLAGFSVLVSSGTLLAAMGTGNPTVAAGALYYMVSSTLTISAFFMLI
HHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHH
ELVERGQDAGANVLAVTMEAYGEGEEEDEEEEVGVTMPATIAVLGACFAACGILLAGLPP
HHHHCCCCCCCCEEEEEEHHHCCCCCCCCHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCC
LSGFIAKFSMLTAILNPSGLGANDSVSTLSWWLVFLIVFAGFASLVSMTRAGIRTFWASI
HHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EGTVPRVLVIEIAPIMLLLGLTLAMTIQAGPVMRYMQETARILDLPASYIQGVISAPRAG
CCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCHHHHHHHHCCCCCC
SNPEAEP
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: Iron [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADH; ubiquinone [C]

Specific reaction: NADH + ubiquinone = NAD+ + ubiquinol [C]

General reaction: Redox reaction [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9680201; 11481430 [H]