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The map label for this gene is artJ [H]

Identifier: 159184329

GI number: 159184329

Start: 467742

End: 468503

Strand: Direct

Name: artJ [H]

Synonym: Atu0479

Alternate gene names: 159184329

Gene position: 467742-468503 (Clockwise)

Preceding gene: 15887823

Following gene: 159184331

Centisome position: 16.46

GC content: 59.84

Gene sequence:

>762_bases
ATGCCGGTGCTGTTCGACGCGCAGGAGCGGTTTCCGGGTGGCGATCTTTCCTCCGTTCCCCGCATCAGGTTTCTGATGTC
GGTGGATTTCCCGCCCTTCAATTTCACCGATCAGGAGGGCCGGCTTGCGGGCTTCCATGTCGATCTGGTGCGGGAAATCT
GCGCCCAGCTGAAGGTGGAAAGCAAATGCCAGGTGCAGGCGCTACCCTTCGACGAGCTGGAAGCGGCGCTCGAAATGGGC
GAGGGCGAAGCGGTGATTTCCGGTCTCGCCACCACGGCCGATCTGCGCAAGAGCTTCACCTTCTCGCGGCCCTATCTGCT
GTTGCCCGCCCGGCTGGCGGTGAACAAGGCGGGGAAATTCACCGGCTCCGGAGCGGATGCGCTTTCGGGAAAGAAGGTGG
GTGTGGTTGGCAATTCGCGGCACGAACAGATGCTGAAAGCGTTTTTTCCAAAAGCCAGGGTCGAAGGGTTCGATGGCTAT
GAGCCGATGTATGAGGCGTTGAAGACAGGCAAGGTGGACGCCATCTTCGCCGACGGTCTGCGCCTGCCTTTCTGGGTTTC
TGGAGGTGCTTCCGAAAGCTGCTGCGCGCTCTTCGGCGGTCCTTATATGTCCGACCGGTTTCTCGGCGAAGGCCTGTCCA
TCATGGCGGTCGATCCGGATAATATACTGGTGCCGGCTTTCGATCAGGCGCTGGCCGCCCTTTCGCGAAATGGCCGGCTG
GAAGAGATTTACCGGCGTTATTTCCCTTACGGGCTTTATTAG

Upstream 100 bases:

>100_bases
AACGCGGAATATAGCCCTCTATTTTACTTTAGTTTTCGCTTTACAATTTGTTGGTTATTTTAGTGAAACGCAGGCCGAAG
GTCTGTCGGATGGAAACCGC

Downstream 100 bases:

>100_bases
AAATCCTGATCGACCGTCGCGACAGTCCTATTTGGCTGGCGCGAAAACCTGTTCATCGCGCCGCAAACGTAGGAGCGCGA
TCCATACGGCGGCGGCGATG

Product: ABC transporter, substrate binding protein (amino acid)

Products: ADP; phosphate; L-arginine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 253; Mature: 252

Protein sequence:

>253_residues
MPVLFDAQERFPGGDLSSVPRIRFLMSVDFPPFNFTDQEGRLAGFHVDLVREICAQLKVESKCQVQALPFDELEAALEMG
EGEAVISGLATTADLRKSFTFSRPYLLLPARLAVNKAGKFTGSGADALSGKKVGVVGNSRHEQMLKAFFPKARVEGFDGY
EPMYEALKTGKVDAIFADGLRLPFWVSGGASESCCALFGGPYMSDRFLGEGLSIMAVDPDNILVPAFDQALAALSRNGRL
EEIYRRYFPYGLY

Sequences:

>Translated_253_residues
MPVLFDAQERFPGGDLSSVPRIRFLMSVDFPPFNFTDQEGRLAGFHVDLVREICAQLKVESKCQVQALPFDELEAALEMG
EGEAVISGLATTADLRKSFTFSRPYLLLPARLAVNKAGKFTGSGADALSGKKVGVVGNSRHEQMLKAFFPKARVEGFDGY
EPMYEALKTGKVDAIFADGLRLPFWVSGGASESCCALFGGPYMSDRFLGEGLSIMAVDPDNILVPAFDQALAALSRNGRL
EEIYRRYFPYGLY
>Mature_252_residues
PVLFDAQERFPGGDLSSVPRIRFLMSVDFPPFNFTDQEGRLAGFHVDLVREICAQLKVESKCQVQALPFDELEAALEMGE
GEAVISGLATTADLRKSFTFSRPYLLLPARLAVNKAGKFTGSGADALSGKKVGVVGNSRHEQMLKAFFPKARVEGFDGYE
PMYEALKTGKVDAIFADGLRLPFWVSGGASESCCALFGGPYMSDRFLGEGLSIMAVDPDNILVPAFDQALAALSRNGRLE
EIYRRYFPYGLY

Specific function: Part of the ABC transporter complex ArtPIQMJ involved in arginine transport. Binds L-arginine with high affinity [H]

COG id: COG0834

COG function: function code ET; ABC-type amino acid transport/signal transduction systems, periplasmic component/domain

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 3 family [H]

Homologues:

Organism=Escherichia coli, GI1787085, Length=239, Percent_Identity=29.2887029288703, Blast_Score=109, Evalue=2e-25,
Organism=Escherichia coli, GI1787088, Length=232, Percent_Identity=28.0172413793103, Blast_Score=108, Evalue=4e-25,
Organism=Escherichia coli, GI1788648, Length=243, Percent_Identity=25.9259259259259, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1788228, Length=219, Percent_Identity=26.4840182648402, Blast_Score=89, Evalue=3e-19,
Organism=Escherichia coli, GI1788649, Length=234, Percent_Identity=25.6410256410256, Blast_Score=84, Evalue=1e-17,

Paralogues:

None

Copy number: 1180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015683
- InterPro:   IPR005768
- InterPro:   IPR001638
- InterPro:   IPR018313 [H]

Pfam domain/function: PF00497 SBP_bac_3 [H]

EC number: NA

Molecular weight: Translated: 27733; Mature: 27602

Theoretical pI: Translated: 5.00; Mature: 5.00

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPVLFDAQERFPGGDLSSVPRIRFLMSVDFPPFNFTDQEGRLAGFHVDLVREICAQLKVE
CCCEECCHHCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCEEEEHHHHHHHHHHHCCCC
SKCQVQALPFDELEAALEMGEGEAVISGLATTADLRKSFTFSRPYLLLPARLAVNKAGKF
CCCEEEECCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCEEEECHHHHHCCCCCC
TGSGADALSGKKVGVVGNSRHEQMLKAFFPKARVEGFDGYEPMYEALKTGKVDAIFADGL
CCCCCCCCCCCEEEEECCCHHHHHHHHHCCHHHCCCCCCHHHHHHHHHCCCCEEEEECCC
RLPFWVSGGASESCCALFGGPYMSDRFLGEGLSIMAVDPDNILVPAFDQALAALSRNGRL
CCCEEECCCCCCCHHHHHCCCCCCCHHHCCCCEEEEECCCCEEECCHHHHHHHHHCCCCH
EEIYRRYFPYGLY
HHHHHHHCCCCCC
>Mature Secondary Structure 
PVLFDAQERFPGGDLSSVPRIRFLMSVDFPPFNFTDQEGRLAGFHVDLVREICAQLKVE
CCEECCHHCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCEEEEHHHHHHHHHHHCCCC
SKCQVQALPFDELEAALEMGEGEAVISGLATTADLRKSFTFSRPYLLLPARLAVNKAGKF
CCCEEEECCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCEEEECHHHHHCCCCCC
TGSGADALSGKKVGVVGNSRHEQMLKAFFPKARVEGFDGYEPMYEALKTGKVDAIFADGL
CCCCCCCCCCCEEEEECCCHHHHHHHHHCCHHHCCCCCCHHHHHHHHHCCCCEEEEECCC
RLPFWVSGGASESCCALFGGPYMSDRFLGEGLSIMAVDPDNILVPAFDQALAALSRNGRL
CCCEEECCCCCCCHHHHHCCCCCCCHHHCCCCEEEEECCCCEEECCHHHHHHHHHCCCCH
EEIYRRYFPYGLY
HHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-arginine [Periplasm]; H2O [C]

Specific reaction: ATP + L-arginine [Periplasm] + H2O = ADP + phosphate + L-arginine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8501075; 8905232; 9278503; 9298646; 9600841 [H]