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The map label for this gene is miaB

Identifier: 159184272

GI number: 159184272

Start: 349679

End: 351046

Strand: Direct

Name: miaB

Synonym: Atu0356

Alternate gene names: 159184272

Gene position: 349679-351046 (Clockwise)

Preceding gene: 15887706

Following gene: 159184273

Centisome position: 12.31

GC content: 59.14

Gene sequence:

>1368_bases
ATGATCGCCCGCGAAGGCTCTAACAGTCGAAAAGTCTTTATCAAGACCTATGGCTGCCAGATGAACGTCTACGATTCCGT
GCGCATGAGCGATGCGCTGGCGAAGGATGGTTACGTTCAGACGGAAGACATGGGCGAGGCGGATCTGGTTCTCCTCAACA
CCTGTCACATCCGCGAGAAGGCCGCGGAGAAGGTTTATTCCGCGCTTGGCCGCCTGCGTGACATGAAAAAGTCGCGCGAA
GAGCAGGGCCGCGAATTCATGATCGGCGTTGCCGGCTGCGTGGCGCAGGCGGAAGGCGAGGAAATTCTTCGCCGTGCGCC
CGCCGTCGATGTCGTGATCGGCCCGCAGACCTATCACCGCCTGCCTGACGCCCTGAAGCGCGTGCGCCGCGGTGAGCGCG
TCATCGAGACCGAATATGCGGTCGAGGACAAGTTCGAGCATCTACCGGTTGCCGAAAAGGCGACGCTGCGCTCGCGTGGC
GTCACGGCGTTTTTGACGGTGCAGGAAGGTTGCGACAAGTTCTGCACCTTCTGTGTCGTACCCTATACACGCGGTTCTGA
AGTGTCGCGCCCCGTTCGCCAGATCGTCGATGAAGCGATGAAACTGGTGGATGCCGGCGTGCGCGAGATCACGCTGCTTG
GCCAGAACGTCAATGCCTGGCAGGGCGAAGGCCCCAAGGGCGAAAAATGGGGTCTGGCCGAACTGCTCTACAGGCTGGCC
GAAATTCCGGGCCTTGCACGGTTGCGCTACACCACCAGCCACCCGCGCGACATGGATGACCGCCTGATCGGCGCCCATCG
CGACCTGCGTATCCTGATGCCCTATCTGCACCTACCGGTGCAGTCCGGCTCGGATCGCATCCTGAAAGCGATGAACCGTC
GCCATACGGGTGAGGAATATATCCAGCTGATCGAGAAAATCCGCTCCGCCCGCCCTGATATTGCCATGTCGGGGGATTTT
ATTGTCGGCTTCCCCGGTGAGACTGATCGCGATTTCGAAGATACGATGGCGATGGTCGAGACGGTGAAATACGCACAGGC
CTTTTCGTTCAAATATTCCACTCGTCCCGGCACGCCGGGGGCGGACCTGACGGACCAGGTGGCGGAAGACGTGAAGGCCG
AGCGGCTGGAACGATTGCAGGCCCTGTTGCTGCGGCAGCAGAAGGAATTTGCGGAATCGCTCGTTGGAAAAACCATGGAT
GTGTTGCTGGAAAAGCCCGGCCGCATGCCTGAGCAGTTGATAGGTCGCTCTCCGTGGCTGCAATCCGTGAATCTTGATGC
AAAGACGTTGAAAATCGGTGACATTGTTAATGTACGAATCACCGCAACGGGTCCAAACAGCTTGTTTGCCGAGGTGGCAG
GGAGTTAG

Upstream 100 bases:

>100_bases
GGCATCCTCGCTTTTCCGGCATTTTTCTGTTTAATCTGGCTATGAAAAACACTAGATAGCCGCCATGACCCAGGAAACAC
TTGGCCTTGACGCCCCACAA

Downstream 100 bases:

>100_bases
AGTGAGGAGCCGACACTGATTGGGACAGGAGCCTGACCGCTTGAACGCACACGAATTGGTATCAAATCCATCGCGCCAGC
CACGCCAAGCCGCGACCGAC

Product: (dimethylallyl)adenosine tRNA methylthiotransferase

Products: NA

Alternate protein names: tRNA-i(6)A37 methylthiotransferase

Number of amino acids: Translated: 455; Mature: 455

Protein sequence:

>455_residues
MIAREGSNSRKVFIKTYGCQMNVYDSVRMSDALAKDGYVQTEDMGEADLVLLNTCHIREKAAEKVYSALGRLRDMKKSRE
EQGREFMIGVAGCVAQAEGEEILRRAPAVDVVIGPQTYHRLPDALKRVRRGERVIETEYAVEDKFEHLPVAEKATLRSRG
VTAFLTVQEGCDKFCTFCVVPYTRGSEVSRPVRQIVDEAMKLVDAGVREITLLGQNVNAWQGEGPKGEKWGLAELLYRLA
EIPGLARLRYTTSHPRDMDDRLIGAHRDLRILMPYLHLPVQSGSDRILKAMNRRHTGEEYIQLIEKIRSARPDIAMSGDF
IVGFPGETDRDFEDTMAMVETVKYAQAFSFKYSTRPGTPGADLTDQVAEDVKAERLERLQALLLRQQKEFAESLVGKTMD
VLLEKPGRMPEQLIGRSPWLQSVNLDAKTLKIGDIVNVRITATGPNSLFAEVAGS

Sequences:

>Translated_455_residues
MIAREGSNSRKVFIKTYGCQMNVYDSVRMSDALAKDGYVQTEDMGEADLVLLNTCHIREKAAEKVYSALGRLRDMKKSRE
EQGREFMIGVAGCVAQAEGEEILRRAPAVDVVIGPQTYHRLPDALKRVRRGERVIETEYAVEDKFEHLPVAEKATLRSRG
VTAFLTVQEGCDKFCTFCVVPYTRGSEVSRPVRQIVDEAMKLVDAGVREITLLGQNVNAWQGEGPKGEKWGLAELLYRLA
EIPGLARLRYTTSHPRDMDDRLIGAHRDLRILMPYLHLPVQSGSDRILKAMNRRHTGEEYIQLIEKIRSARPDIAMSGDF
IVGFPGETDRDFEDTMAMVETVKYAQAFSFKYSTRPGTPGADLTDQVAEDVKAERLERLQALLLRQQKEFAESLVGKTMD
VLLEKPGRMPEQLIGRSPWLQSVNLDAKTLKIGDIVNVRITATGPNSLFAEVAGS
>Mature_455_residues
MIAREGSNSRKVFIKTYGCQMNVYDSVRMSDALAKDGYVQTEDMGEADLVLLNTCHIREKAAEKVYSALGRLRDMKKSRE
EQGREFMIGVAGCVAQAEGEEILRRAPAVDVVIGPQTYHRLPDALKRVRRGERVIETEYAVEDKFEHLPVAEKATLRSRG
VTAFLTVQEGCDKFCTFCVVPYTRGSEVSRPVRQIVDEAMKLVDAGVREITLLGQNVNAWQGEGPKGEKWGLAELLYRLA
EIPGLARLRYTTSHPRDMDDRLIGAHRDLRILMPYLHLPVQSGSDRILKAMNRRHTGEEYIQLIEKIRSARPDIAMSGDF
IVGFPGETDRDFEDTMAMVETVKYAQAFSFKYSTRPGTPGADLTDQVAEDVKAERLERLQALLLRQQKEFAESLVGKTMD
VLLEKPGRMPEQLIGRSPWLQSVNLDAKTLKIGDIVNVRITATGPNSLFAEVAGS

Specific function: Catalyzes the methylthiolation of N6- (dimethylallyl)adenosine (i(6)A), leading to the formation of 2- methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at position 37 in tRNAs that read codons beginning with uridine

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain

Homologues:

Organism=Homo sapiens, GI28872784, Length=479, Percent_Identity=34.446764091858, Blast_Score=282, Evalue=6e-76,
Organism=Homo sapiens, GI28872782, Length=479, Percent_Identity=34.446764091858, Blast_Score=281, Evalue=9e-76,
Organism=Homo sapiens, GI93277076, Length=442, Percent_Identity=28.5067873303167, Blast_Score=171, Evalue=1e-42,
Organism=Escherichia coli, GI1786882, Length=449, Percent_Identity=48.3296213808463, Blast_Score=409, Evalue=1e-115,
Organism=Escherichia coli, GI1787057, Length=451, Percent_Identity=31.7073170731707, Blast_Score=187, Evalue=9e-49,
Organism=Caenorhabditis elegans, GI17553146, Length=422, Percent_Identity=33.8862559241706, Blast_Score=251, Evalue=8e-67,
Organism=Caenorhabditis elegans, GI71996771, Length=371, Percent_Identity=26.4150943396226, Blast_Score=126, Evalue=2e-29,
Organism=Drosophila melanogaster, GI21356207, Length=478, Percent_Identity=32.6359832635983, Blast_Score=265, Evalue=4e-71,
Organism=Drosophila melanogaster, GI19922432, Length=456, Percent_Identity=28.2894736842105, Blast_Score=164, Evalue=1e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MIAB_AGRT5 (Q7D1M2)

Other databases:

- EMBL:   AE007869
- RefSeq:   NP_353388.2
- ProteinModelPortal:   Q7D1M2
- SMR:   Q7D1M2
- STRING:   Q7D1M2
- GeneID:   1132394
- GenomeReviews:   AE007869_GR
- KEGG:   atu:Atu0356
- eggNOG:   COG0621
- HOGENOM:   HBG457663
- OMA:   ANLHDDT
- PhylomeDB:   Q7D1M2
- ProtClustDB:   PRK14325
- GO:   GO:0005737
- HAMAP:   MF_01864
- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR006463
- InterPro:   IPR007197
- InterPro:   IPR002792
- PANTHER:   PTHR11918
- SMART:   SM00729
- TIGRFAMs:   TIGR01574
- TIGRFAMs:   TIGR00089

Pfam domain/function: PF04055 Radical_SAM; PF01938 TRAM; PF00919 UPF0004

EC number: NA

Molecular weight: Translated: 51009; Mature: 51009

Theoretical pI: Translated: 6.88; Mature: 6.88

Prosite motif: PS51449 MTTASE_N; PS01278 MTTASE_RADICAL; PS50926 TRAM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIAREGSNSRKVFIKTYGCQMNVYDSVRMSDALAKDGYVQTEDMGEADLVLLNTCHIREK
CCCCCCCCCCEEEEEEECCEEECHHHHHHHHHHHCCCCEECCCCCCCCEEEEEHHHHHHH
AAEKVYSALGRLRDMKKSREEQGREFMIGVAGCVAQAEGEEILRRAPAVDVVIGPQTYHR
HHHHHHHHHHHHHHHHHHHHHCCCEEEEEHHHHHHHCCHHHHHHHCCCCEEEECCHHHHH
LPDALKRVRRGERVIETEYAVEDKFEHLPVAEKATLRSRGVTAFLTVQEGCDKFCTFCVV
HHHHHHHHHCCCHHHHHHHHHHHHHHCCCCHHHHHHHHCCCEEEEEHHHHHHHHHHEEEE
PYTRGSEVSRPVRQIVDEAMKLVDAGVREITLLGQNVNAWQGEGPKGEKWGLAELLYRLA
CCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHH
EIPGLARLRYTTSHPRDMDDRLIGAHRDLRILMPYLHLPVQSGSDRILKAMNRRHTGEEY
HCCCCEEEEECCCCCCCCHHHHHCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHCCCHHHH
IQLIEKIRSARPDIAMSGDFIVGFPGETDRDFEDTMAMVETVKYAQAFSFKYSTRPGTPG
HHHHHHHHHCCCCEEECCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHEEECCCCCCCC
ADLTDQVAEDVKAERLERLQALLLRQQKEFAESLVGKTMDVLLEKPGRMPEQLIGRSPWL
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCCC
QSVNLDAKTLKIGDIVNVRITATGPNSLFAEVAGS
CCCCCCCCEEEECCEEEEEEEECCCCHHHHHHCCC
>Mature Secondary Structure
MIAREGSNSRKVFIKTYGCQMNVYDSVRMSDALAKDGYVQTEDMGEADLVLLNTCHIREK
CCCCCCCCCCEEEEEEECCEEECHHHHHHHHHHHCCCCEECCCCCCCCEEEEEHHHHHHH
AAEKVYSALGRLRDMKKSREEQGREFMIGVAGCVAQAEGEEILRRAPAVDVVIGPQTYHR
HHHHHHHHHHHHHHHHHHHHHCCCEEEEEHHHHHHHCCHHHHHHHCCCCEEEECCHHHHH
LPDALKRVRRGERVIETEYAVEDKFEHLPVAEKATLRSRGVTAFLTVQEGCDKFCTFCVV
HHHHHHHHHCCCHHHHHHHHHHHHHHCCCCHHHHHHHHCCCEEEEEHHHHHHHHHHEEEE
PYTRGSEVSRPVRQIVDEAMKLVDAGVREITLLGQNVNAWQGEGPKGEKWGLAELLYRLA
CCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHH
EIPGLARLRYTTSHPRDMDDRLIGAHRDLRILMPYLHLPVQSGSDRILKAMNRRHTGEEY
HCCCCEEEEECCCCCCCCHHHHHCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHCCCHHHH
IQLIEKIRSARPDIAMSGDFIVGFPGETDRDFEDTMAMVETVKYAQAFSFKYSTRPGTPG
HHHHHHHHHCCCCEEECCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHEEECCCCCCCC
ADLTDQVAEDVKAERLERLQALLLRQQKEFAESLVGKTMDVLLEKPGRMPEQLIGRSPWL
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCCC
QSVNLDAKTLKIGDIVNVRITATGPNSLFAEVAGS
CCCCCCCCEEEECCEEEEEEEECCCCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11743193; 11743194