Click here to switch to the map view.

The map label for this gene is citE [H]

Identifier: 15890035

GI number: 15890035

Start: 2790228

End: 2791112

Strand: Reverse

Name: citE [H]

Synonym: Atu2788

Alternate gene names: 15890035

Gene position: 2791112-2790228 (Counterclockwise)

Preceding gene: 159185384

Following gene: 159185382

Centisome position: 98.22

GC content: 57.29

Gene sequence:

>885_bases
GTGAGACCACGTCGTTCCCTGCTGTCAGTTCCGGCCATCAATATCCGGGCACTCGAAAAAATCCGGGAGCTCGATTGTGA
TGGCGTGATCCTCGATCTAGAGGATTCGGTTGCGCCCGATATGAAGGGCAAGGCGCGGGAAAATCTGGAAAAGCTGTTCT
CAGGCCCGCCTTTCGACGGACGCGAAACCATTATCCGCATCAATCCGCTTTCGACACCTGATGGAAAGGCGGATCTGCAG
CTTGTGCTTTCCTGCCGGCCAGATGCGGTTTTATTGCCGAAGGTGGAACAGCCCTCCGATATTCATGACGTGGCCGATTT
TCTCGCCGAGGCGGATGCGCCCGAGCATCTGAAAATCTGGGCGATGATCGAGACACCGCTTGGTGTCCTGAATGCTGCCT
CGATTGCCGACGCTGCCCATACGCCGAATGCCCGGCTTGCCGCTTTCGTCATCGGGCTCAACGATCTGCGCAAGGAAACG
CATGTCCCACGCCTTCCTGGCCGCACCTATCTCGTGCCGTGGATGATGCAGGTGATTCTTGCCGCGCGGGCCTACGGGCT
CGATGTCATTGACAGCGTCTCGAATGATTTTCGCGACATCGAGGCTTTCGAAGCCGAATGCGAACAGGGGCGCGCCATGG
GCTTCGATGGCAAGATGCTGATCCATCCGGCCCAGATCGAAGCGGCCAACCGGCACTTCGGTCCGGATGAGGCGACGCTT
GCGGAAGCACGCGCGATTATTGCCGCTTTCGCAAAACCGGAATCTGTGGAACTGAACATCATCAACATGAACGGTCAGAT
GATCGAACGGCTGCATGTGGGGCAAGCCGAAAGGCTGGTCGCCATGGCCGATATCATCGCACAAAGAAAGGCAAAAAAGA
CGTGA

Upstream 100 bases:

>100_bases
TTCTACCGGCTTGCAAGAGGATTCAGTGGATTGTCAGCTTGCAGATGTCACAAAAGCTTGACACAAGTGCGGCATCGTCA
ATTTTTCCGAAAATATTTCT

Downstream 100 bases:

>100_bases
AGGTCTATCGTTTCATAACCGGTCCCGACGACGCGAAATTCTGTCACCGGGTCACCGAAGCCCTGAACAAGGGATGGGAA
CTTGCGGGTTCACCGAGCTA

Product: citrate lyase, beta subunit

Products: acetyl-CoA; oxaloacetate

Alternate protein names: (3S)-malyl-CoA thioesterase [H]

Number of amino acids: Translated: 294; Mature: 294

Protein sequence:

>294_residues
MRPRRSLLSVPAINIRALEKIRELDCDGVILDLEDSVAPDMKGKARENLEKLFSGPPFDGRETIIRINPLSTPDGKADLQ
LVLSCRPDAVLLPKVEQPSDIHDVADFLAEADAPEHLKIWAMIETPLGVLNAASIADAAHTPNARLAAFVIGLNDLRKET
HVPRLPGRTYLVPWMMQVILAARAYGLDVIDSVSNDFRDIEAFEAECEQGRAMGFDGKMLIHPAQIEAANRHFGPDEATL
AEARAIIAAFAKPESVELNIINMNGQMIERLHVGQAERLVAMADIIAQRKAKKT

Sequences:

>Translated_294_residues
MRPRRSLLSVPAINIRALEKIRELDCDGVILDLEDSVAPDMKGKARENLEKLFSGPPFDGRETIIRINPLSTPDGKADLQ
LVLSCRPDAVLLPKVEQPSDIHDVADFLAEADAPEHLKIWAMIETPLGVLNAASIADAAHTPNARLAAFVIGLNDLRKET
HVPRLPGRTYLVPWMMQVILAARAYGLDVIDSVSNDFRDIEAFEAECEQGRAMGFDGKMLIHPAQIEAANRHFGPDEATL
AEARAIIAAFAKPESVELNIINMNGQMIERLHVGQAERLVAMADIIAQRKAKKT
>Mature_294_residues
MRPRRSLLSVPAINIRALEKIRELDCDGVILDLEDSVAPDMKGKARENLEKLFSGPPFDGRETIIRINPLSTPDGKADLQ
LVLSCRPDAVLLPKVEQPSDIHDVADFLAEADAPEHLKIWAMIETPLGVLNAASIADAAHTPNARLAAFVIGLNDLRKET
HVPRLPGRTYLVPWMMQVILAARAYGLDVIDSVSNDFRDIEAFEAECEQGRAMGFDGKMLIHPAQIEAANRHFGPDEATL
AEARAIIAAFAKPESVELNIINMNGQMIERLHVGQAERLVAMADIIAQRKAKKT

Specific function: Catalyzes the hydrolysis of (3S)-malyl-CoA to (3S)- malate and free CoA. Inactive towards beta-methylmalyl-CoA and other CoA esters [H]

COG id: COG2301

COG function: function code G; Citrate lyase beta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HpcH/HpaI aldolase family [H]

Homologues:

Organism=Homo sapiens, GI45545437, Length=297, Percent_Identity=29.2929292929293, Blast_Score=134, Evalue=1e-31,
Organism=Escherichia coli, GI87081764, Length=294, Percent_Identity=29.5918367346939, Blast_Score=119, Evalue=3e-28,
Organism=Caenorhabditis elegans, GI17557458, Length=299, Percent_Identity=27.0903010033445, Blast_Score=103, Evalue=8e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005000
- InterPro:   IPR011206
- InterPro:   IPR015813 [H]

Pfam domain/function: PF03328 HpcH_HpaI [H]

EC number: 4.1.3.34; 4.1.3.6

Molecular weight: Translated: 32329; Mature: 32329

Theoretical pI: Translated: 5.11; Mature: 5.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRPRRSLLSVPAINIRALEKIRELDCDGVILDLEDSVAPDMKGKARENLEKLFSGPPFDG
CCCCHHHHCCCCCHHHHHHHHHHCCCCCEEEECCCCCCCCCCCHHHHHHHHHHCCCCCCC
RETIIRINPLSTPDGKADLQLVLSCRPDAVLLPKVEQPSDIHDVADFLAEADAPEHLKIW
CCEEEEEECCCCCCCCCCEEEEEECCCCEEEECCCCCCCCHHHHHHHHHHCCCCCCEEEE
AMIETPLGVLNAASIADAAHTPNARLAAFVIGLNDLRKETHVPRLPGRTYLVPWMMQVIL
EEECCCCCHHHHHHHHHHCCCCCCEEEEEEECHHHHHHHHCCCCCCCCEEHHHHHHHHHH
AARAYGLDVIDSVSNDFRDIEAFEAECEQGRAMGFDGKMLIHPAQIEAANRHFGPDEATL
HHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCEECCCCEEEEEHHHHHHCCCCCCCCHHHH
AEARAIIAAFAKPESVELNIINMNGQMIERLHVGQAERLVAMADIIAQRKAKKT
HHHHHHHHHHCCCCCEEEEEEECCHHHHHHHHCCHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MRPRRSLLSVPAINIRALEKIRELDCDGVILDLEDSVAPDMKGKARENLEKLFSGPPFDG
CCCCHHHHCCCCCHHHHHHHHHHCCCCCEEEECCCCCCCCCCCHHHHHHHHHHCCCCCCC
RETIIRINPLSTPDGKADLQLVLSCRPDAVLLPKVEQPSDIHDVADFLAEADAPEHLKIW
CCEEEEEECCCCCCCCCCEEEEEECCCCEEEECCCCCCCCHHHHHHHHHHCCCCCCEEEE
AMIETPLGVLNAASIADAAHTPNARLAAFVIGLNDLRKETHVPRLPGRTYLVPWMMQVIL
EEECCCCCHHHHHHHHHHCCCCCCEEEEEEECHHHHHHHHCCCCCCCCEEHHHHHHHHHH
AARAYGLDVIDSVSNDFRDIEAFEAECEQGRAMGFDGKMLIHPAQIEAANRHFGPDEATL
HHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCEECCCCEEEEEHHHHHHCCCCCCCCHHHH
AEARAIIAAFAKPESVELNIINMNGQMIERLHVGQAERLVAMADIIAQRKAKKT
HHHHHHHHHHCCCCCEEEEEEECCHHHHHHHHCCHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: (3S)-citryl-CoA

Specific reaction: (3S)-citryl-CoA = acetyl-CoA + oxaloacetate

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA