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The map label for this gene is rpoH [H]

Identifier: 15889716

GI number: 15889716

Start: 2415172

End: 2416074

Strand: Direct

Name: rpoH [H]

Synonym: Atu2445

Alternate gene names: 15889716

Gene position: 2415172-2416074 (Clockwise)

Preceding gene: 15889715

Following gene: 15889719

Centisome position: 84.99

GC content: 60.47

Gene sequence:

>903_bases
ATGGCCCGCAATAGTTTGCCTACGATCACAGCCGGCGAAGCCGGTCTCAATAGATATCTCGACGAAATTCGTAAGTTCCC
GATGCTGGAGCCGCAGGAAGAGTACATGCTTGGCAAGCGTTATGCCGAGCATGGCGATCGCGACGCCGCGCATAAACTCG
TCACCAGCCATCTGCGTCTCGTCGCCAAGATCGCCATGGGTTACCGCGGTTACGGCCTGCCGATCGGCGAAGTCGTGTCC
GAAGGCAATGTCGGCCTGATGCAGGCGGTGAAGAAGTTCGATCCGGAACGCGGTTTCCGTCTGGCCACCTATGCCATGTG
GTGGATCAAGGCCTCGATCCAGGAATATATCCTGCGTTCGTGGTCTCTGGTGAAGATGGGCACGACGGCCAACCAGAAAC
GCCTGTTCTTCAACCTGCGCCGGCTGAAAGGCCGCATCCAGGCGATTGACGACGGCGATCTGAAGCCGGAACACGTCAAG
GAAATCGCCACCAAGCTGCAGGTGTCGGAAGAAGAAGTCATCTCGATGAACCGCCGCCTGCATGGCGACGCCTCGCTGAA
TGCGCCGATCAAGGCGTCCGAAGGCGAGTCCGGCCAATGGCAGGACTGGCTGGTGGATGACCATGAGAGCCAGGAAGCCG
TGCTGATCGAGCAGGACGAGCTTGAAACGCGTCGCCGCATGTTGGCCAAAGCCATGGGCGTGCTGAATGAGCGCGAACGC
CGCATCTTCGAGGCCCGCCGCCTCGCCGAAGATCCGGTGACGCTGGAAGAACTCTCATCCGAGTTCGACATCAGCCGCGA
ACGCGTGCGCCAGATCGAGGTTCGCGCCTTCGAGAAGGTTCAGGAAGCGGTGCAGAAGGAAGCGCTCGAAGCCGCCCGCG
CATTGCGCGTGGTGGACGCGTAA

Upstream 100 bases:

>100_bases
GCGGGGTCGGTAACGACCCGCAGCTTACGGTGCGCCTCGGGCGGGTGATTTTCCACGCCAAAGAGGGTACCGTTCCAGAC
CATAGATAAGGGGGTGCTTT

Downstream 100 bases:

>100_bases
GGCAGCGCGCTCTTGACGCCAATGCGCTGACCATGAATCATCCTCGGCCTTCGGCCGGGGATTTTTGTTTGTAAAAGGCG
GGTGATGGGAAATGGTCTGC

Product: RNA polymerase factor sigma-32

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 300; Mature: 299

Protein sequence:

>300_residues
MARNSLPTITAGEAGLNRYLDEIRKFPMLEPQEEYMLGKRYAEHGDRDAAHKLVTSHLRLVAKIAMGYRGYGLPIGEVVS
EGNVGLMQAVKKFDPERGFRLATYAMWWIKASIQEYILRSWSLVKMGTTANQKRLFFNLRRLKGRIQAIDDGDLKPEHVK
EIATKLQVSEEEVISMNRRLHGDASLNAPIKASEGESGQWQDWLVDDHESQEAVLIEQDELETRRRMLAKAMGVLNERER
RIFEARRLAEDPVTLEELSSEFDISRERVRQIEVRAFEKVQEAVQKEALEAARALRVVDA

Sequences:

>Translated_300_residues
MARNSLPTITAGEAGLNRYLDEIRKFPMLEPQEEYMLGKRYAEHGDRDAAHKLVTSHLRLVAKIAMGYRGYGLPIGEVVS
EGNVGLMQAVKKFDPERGFRLATYAMWWIKASIQEYILRSWSLVKMGTTANQKRLFFNLRRLKGRIQAIDDGDLKPEHVK
EIATKLQVSEEEVISMNRRLHGDASLNAPIKASEGESGQWQDWLVDDHESQEAVLIEQDELETRRRMLAKAMGVLNERER
RIFEARRLAEDPVTLEELSSEFDISRERVRQIEVRAFEKVQEAVQKEALEAARALRVVDA
>Mature_299_residues
ARNSLPTITAGEAGLNRYLDEIRKFPMLEPQEEYMLGKRYAEHGDRDAAHKLVTSHLRLVAKIAMGYRGYGLPIGEVVSE
GNVGLMQAVKKFDPERGFRLATYAMWWIKASIQEYILRSWSLVKMGTTANQKRLFFNLRRLKGRIQAIDDGDLKPEHVKE
IATKLQVSEEEVISMNRRLHGDASLNAPIKASEGESGQWQDWLVDDHESQEAVLIEQDELETRRRMLAKAMGVLNERERR
IFEARRLAEDPVTLEELSSEFDISRERVRQIEVRAFEKVQEAVQKEALEAARALRVVDA

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is responsible for the expression of heat shock promoters [H]

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family. Sigma-32 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789871, Length=285, Percent_Identity=37.5438596491228, Blast_Score=182, Evalue=3e-47,
Organism=Escherichia coli, GI1789098, Length=274, Percent_Identity=32.8467153284672, Blast_Score=124, Evalue=6e-30,
Organism=Escherichia coli, GI1789448, Length=251, Percent_Identity=29.8804780876494, Blast_Score=94, Evalue=1e-20,

Paralogues:

None

Copy number: <10 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR012759
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 34433; Mature: 34302

Theoretical pI: Translated: 6.54; Mature: 6.54

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARNSLPTITAGEAGLNRYLDEIRKFPMLEPQEEYMLGKRYAEHGDRDAAHKLVTSHLRL
CCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
VAKIAMGYRGYGLPIGEVVSEGNVGLMQAVKKFDPERGFRLATYAMWWIKASIQEYILRS
HHHHHHCCCCCCCCHHHHHCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHC
WSLVKMGTTANQKRLFFNLRRLKGRIQAIDDGDLKPEHVKEIATKLQVSEEEVISMNRRL
CHHEEECCCCCHHHHHHHHHHHHHHHEECCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHC
HGDASLNAPIKASEGESGQWQDWLVDDHESQEAVLIEQDELETRRRMLAKAMGVLNERER
CCCCCCCCCEECCCCCCCCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHH
RIFEARRLAEDPVTLEELSSEFDISRERVRQIEVRAFEKVQEAVQKEALEAARALRVVDA
HHHHHHHHCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
ARNSLPTITAGEAGLNRYLDEIRKFPMLEPQEEYMLGKRYAEHGDRDAAHKLVTSHLRL
CCCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
VAKIAMGYRGYGLPIGEVVSEGNVGLMQAVKKFDPERGFRLATYAMWWIKASIQEYILRS
HHHHHHCCCCCCCCHHHHHCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHC
WSLVKMGTTANQKRLFFNLRRLKGRIQAIDDGDLKPEHVKEIATKLQVSEEEVISMNRRL
CHHEEECCCCCHHHHHHHHHHHHHHHEECCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHC
HGDASLNAPIKASEGESGQWQDWLVDDHESQEAVLIEQDELETRRRMLAKAMGVLNERER
CCCCCCCCCEECCCCCCCCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHH
RIFEARRLAEDPVTLEELSSEFDISRERVRQIEVRAFEKVQEAVQKEALEAARALRVVDA
HHHHHHHHCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7501460 [H]