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The map label for this gene is tuf [H]

Identifier: 15889258

GI number: 15889258

Start: 1936382

End: 1937557

Strand: Reverse

Name: tuf [H]

Synonym: Atu1966

Alternate gene names: 15889258

Gene position: 1937557-1936382 (Counterclockwise)

Preceding gene: 159184995

Following gene: 159184993

Centisome position: 68.19

GC content: 58.67

Gene sequence:

>1176_bases
ATGGCAAAGAGCAAGTTTGAGCGCAATAAGCCGCACGTCAACATTGGCACGATCGGTCACGTCGACCACGGCAAGACGTC
GTTGACTGCTGCGATCACGAAGTATTTCGGCGAGTTCAAGGCGTATGACCAAATCGACGCCGCTCCTGAAGAAAAGGCTC
GTGGTATCACGATTTCGACGGCGCACGTTGAGTATGAGACGCCTGCGCGTCACTACGCCCACGTTGACTGCCCCGGCCAC
GCCGACTATGTGAAGAACATGATCACCGGTGCTGCCCAGATGGACGGCGCGATCCTGGTTTGCTCGGCTGCCGACGGCCC
GATGCCACAGACCCGCGAGCACATCCTGCTTGCCCGTCAGGTTGGCGTTCCGGCCATCGTCGTGTTCCTCAACAAGGTCG
ACCAGGTTGACGACGCCGAGCTTCTCGAGCTCGTCGAGCTTGAAGTTCGCGAACTTCTGTCGTCCTACGACTTCCCGGGC
GACGATATCCCGATCATCAAGGGTTCGGCACTTGCTGCTCTTGAAGATTCTGACAAGAAGATCGGTGAAGACGCGATCCG
CGAGCTGATGGCTGCTGTCGACGCCTACATCCCGACGCCCGAGCGTCCGATCGACCAGCCGTTCCTGATGCCGATCGAAG
ACGTGTTCTCGATCTCGGGTCGTGGTACGGTTGTGACGGGTCGCGTTGAGCGCGGTATCGTCAAGGTTGGTGAAGAAGTC
GAAATCGTCGGCATCCGTCCGACCTCGAAGACGACTGTTACCGGCGTTGAAATGTTCCGCAAGCTGCTCGACCAGGGCCA
GGCCGGCGACAACATCGGTGCACTCGTTCGCGGCGTTACCCGTGACGGCGTCGAGCGTGGTCAGATCCTGTGCAAGCCGG
GTTCGGTCAAGCCGCACAAGAAGTTCATGGCAGAAGCCTACATCCTGACGAAGGAAGAAGGCGGTCGTCATACGCCGTTC
TTCACGAACTACCGTCCGCAGTTCTACTTCCGTACGACTGACGTTACCGGTATCGTTTCGCTTCCTGAAGGCACGGAAAT
GGTTATGCCTGGCGACAACGTCACTGTTGAAGTCGAGCTGATCGTTCCGATCGCGATGGAAGAAAAGCTGCGCTTCGCTA
TCCGCGAAGGCGGCCGTACCGTCGGCGCCGGCATCGTTGCTTCGATCGTCGAGTAA

Upstream 100 bases:

>100_bases
TTCACAATTAAGTCTTGCGCATTCGCTTACAAAGCCTTAAACGGCGACACCAAACCGGTTCGCCGGGAAATCGACTTCAC
GTTCACAGGAAGCAATTCAC

Downstream 100 bases:

>100_bases
TTTTCAGGCCTGAAAAGGCTTGAAGGAAACCCCGCTGGAAACAGCGGGGTTTTTTGTTTTGTATTGGGGCTTGGAAGAGG
GGTGGCTTTCGCTCTTCGGC

Product: elongation factor Tu

Products: GDP; phosphate

Alternate protein names: EF-Tu 2 [H]

Number of amino acids: Translated: 391; Mature: 390

Protein sequence:

>391_residues
MAKSKFERNKPHVNIGTIGHVDHGKTSLTAAITKYFGEFKAYDQIDAAPEEKARGITISTAHVEYETPARHYAHVDCPGH
ADYVKNMITGAAQMDGAILVCSAADGPMPQTREHILLARQVGVPAIVVFLNKVDQVDDAELLELVELEVRELLSSYDFPG
DDIPIIKGSALAALEDSDKKIGEDAIRELMAAVDAYIPTPERPIDQPFLMPIEDVFSISGRGTVVTGRVERGIVKVGEEV
EIVGIRPTSKTTVTGVEMFRKLLDQGQAGDNIGALVRGVTRDGVERGQILCKPGSVKPHKKFMAEAYILTKEEGGRHTPF
FTNYRPQFYFRTTDVTGIVSLPEGTEMVMPGDNVTVEVELIVPIAMEEKLRFAIREGGRTVGAGIVASIVE

Sequences:

>Translated_391_residues
MAKSKFERNKPHVNIGTIGHVDHGKTSLTAAITKYFGEFKAYDQIDAAPEEKARGITISTAHVEYETPARHYAHVDCPGH
ADYVKNMITGAAQMDGAILVCSAADGPMPQTREHILLARQVGVPAIVVFLNKVDQVDDAELLELVELEVRELLSSYDFPG
DDIPIIKGSALAALEDSDKKIGEDAIRELMAAVDAYIPTPERPIDQPFLMPIEDVFSISGRGTVVTGRVERGIVKVGEEV
EIVGIRPTSKTTVTGVEMFRKLLDQGQAGDNIGALVRGVTRDGVERGQILCKPGSVKPHKKFMAEAYILTKEEGGRHTPF
FTNYRPQFYFRTTDVTGIVSLPEGTEMVMPGDNVTVEVELIVPIAMEEKLRFAIREGGRTVGAGIVASIVE
>Mature_390_residues
AKSKFERNKPHVNIGTIGHVDHGKTSLTAAITKYFGEFKAYDQIDAAPEEKARGITISTAHVEYETPARHYAHVDCPGHA
DYVKNMITGAAQMDGAILVCSAADGPMPQTREHILLARQVGVPAIVVFLNKVDQVDDAELLELVELEVRELLSSYDFPGD
DIPIIKGSALAALEDSDKKIGEDAIRELMAAVDAYIPTPERPIDQPFLMPIEDVFSISGRGTVVTGRVERGIVKVGEEVE
IVGIRPTSKTTVTGVEMFRKLLDQGQAGDNIGALVRGVTRDGVERGQILCKPGSVKPHKKFMAEAYILTKEEGGRHTPFF
TNYRPQFYFRTTDVTGIVSLPEGTEMVMPGDNVTVEVELIVPIAMEEKLRFAIREGGRTVGAGIVASIVE

Specific function: This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis [H]

COG id: COG0050

COG function: function code J; GTPases - translation elongation factors

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily [H]

Homologues:

Organism=Homo sapiens, GI34147630, Length=394, Percent_Identity=55.8375634517767, Blast_Score=474, Evalue=1e-134,
Organism=Homo sapiens, GI223555963, Length=440, Percent_Identity=28.4090909090909, Blast_Score=165, Evalue=5e-41,
Organism=Homo sapiens, GI5729864, Length=440, Percent_Identity=28.4090909090909, Blast_Score=165, Evalue=8e-41,
Organism=Homo sapiens, GI4503475, Length=448, Percent_Identity=28.3482142857143, Blast_Score=158, Evalue=8e-39,
Organism=Homo sapiens, GI4503471, Length=448, Percent_Identity=28.3482142857143, Blast_Score=157, Evalue=2e-38,
Organism=Homo sapiens, GI54607086, Length=313, Percent_Identity=28.1150159744409, Blast_Score=118, Evalue=8e-27,
Organism=Homo sapiens, GI194018520, Length=320, Percent_Identity=29.375, Blast_Score=112, Evalue=9e-25,
Organism=Homo sapiens, GI194018522, Length=320, Percent_Identity=29.375, Blast_Score=111, Evalue=9e-25,
Organism=Homo sapiens, GI194097354, Length=320, Percent_Identity=29.375, Blast_Score=111, Evalue=9e-25,
Organism=Homo sapiens, GI46094014, Length=322, Percent_Identity=30.4347826086957, Blast_Score=111, Evalue=1e-24,
Organism=Homo sapiens, GI4503507, Length=269, Percent_Identity=27.5092936802974, Blast_Score=77, Evalue=3e-14,
Organism=Homo sapiens, GI157426893, Length=290, Percent_Identity=26.8965517241379, Blast_Score=65, Evalue=1e-10,
Organism=Escherichia coli, GI1789737, Length=395, Percent_Identity=74.4303797468354, Blast_Score=582, Evalue=1e-167,
Organism=Escherichia coli, GI1790412, Length=395, Percent_Identity=74.4303797468354, Blast_Score=582, Evalue=1e-167,
Organism=Escherichia coli, GI2367247, Length=374, Percent_Identity=31.5508021390374, Blast_Score=142, Evalue=3e-35,
Organism=Escherichia coli, GI48994988, Length=237, Percent_Identity=33.3333333333333, Blast_Score=105, Evalue=5e-24,
Organism=Escherichia coli, GI1789108, Length=211, Percent_Identity=30.8056872037915, Blast_Score=79, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI17556456, Length=402, Percent_Identity=50.9950248756219, Blast_Score=399, Evalue=1e-111,
Organism=Caenorhabditis elegans, GI25141371, Length=387, Percent_Identity=43.6692506459948, Blast_Score=323, Evalue=1e-88,
Organism=Caenorhabditis elegans, GI17552884, Length=451, Percent_Identity=30.820399113082, Blast_Score=167, Evalue=7e-42,
Organism=Caenorhabditis elegans, GI17569207, Length=451, Percent_Identity=30.820399113082, Blast_Score=167, Evalue=7e-42,
Organism=Caenorhabditis elegans, GI32566303, Length=431, Percent_Identity=29.0023201856148, Blast_Score=145, Evalue=3e-35,
Organism=Caenorhabditis elegans, GI17506081, Length=297, Percent_Identity=29.96632996633, Blast_Score=122, Evalue=3e-28,
Organism=Caenorhabditis elegans, GI115532067, Length=441, Percent_Identity=26.0770975056689, Blast_Score=119, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI115532065, Length=441, Percent_Identity=26.0770975056689, Blast_Score=119, Evalue=4e-27,
Organism=Caenorhabditis elegans, GI32566629, Length=438, Percent_Identity=25.1141552511416, Blast_Score=117, Evalue=8e-27,
Organism=Caenorhabditis elegans, GI32566301, Length=166, Percent_Identity=33.7349397590361, Blast_Score=88, Evalue=9e-18,
Organism=Caenorhabditis elegans, GI17509919, Length=277, Percent_Identity=25.9927797833935, Blast_Score=77, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17557151, Length=231, Percent_Identity=26.4069264069264, Blast_Score=69, Evalue=5e-12,
Organism=Saccharomyces cerevisiae, GI6324761, Length=398, Percent_Identity=65.8291457286432, Blast_Score=528, Evalue=1e-151,
Organism=Saccharomyces cerevisiae, GI6325337, Length=443, Percent_Identity=30.2483069977427, Blast_Score=163, Evalue=3e-41,
Organism=Saccharomyces cerevisiae, GI6319594, Length=443, Percent_Identity=30.2483069977427, Blast_Score=163, Evalue=3e-41,
Organism=Saccharomyces cerevisiae, GI6322937, Length=273, Percent_Identity=27.1062271062271, Blast_Score=89, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6320377, Length=339, Percent_Identity=28.6135693215339, Blast_Score=86, Evalue=8e-18,
Organism=Saccharomyces cerevisiae, GI6320863, Length=269, Percent_Identity=27.5092936802974, Blast_Score=75, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6323098, Length=137, Percent_Identity=30.6569343065693, Blast_Score=65, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6323320, Length=244, Percent_Identity=27.8688524590164, Blast_Score=64, Evalue=5e-11,
Organism=Drosophila melanogaster, GI281363316, Length=393, Percent_Identity=58.2697201017812, Blast_Score=482, Evalue=1e-136,
Organism=Drosophila melanogaster, GI17864358, Length=393, Percent_Identity=58.2697201017812, Blast_Score=482, Evalue=1e-136,
Organism=Drosophila melanogaster, GI19921738, Length=384, Percent_Identity=49.7395833333333, Blast_Score=395, Evalue=1e-110,
Organism=Drosophila melanogaster, GI45553807, Length=450, Percent_Identity=28.6666666666667, Blast_Score=157, Evalue=1e-38,
Organism=Drosophila melanogaster, GI45553816, Length=450, Percent_Identity=28.6666666666667, Blast_Score=157, Evalue=1e-38,
Organism=Drosophila melanogaster, GI24651721, Length=450, Percent_Identity=28.6666666666667, Blast_Score=157, Evalue=1e-38,
Organism=Drosophila melanogaster, GI17864154, Length=450, Percent_Identity=28.6666666666667, Blast_Score=157, Evalue=1e-38,
Organism=Drosophila melanogaster, GI24652838, Length=450, Percent_Identity=29.3333333333333, Blast_Score=154, Evalue=7e-38,
Organism=Drosophila melanogaster, GI17137572, Length=450, Percent_Identity=29.3333333333333, Blast_Score=154, Evalue=7e-38,
Organism=Drosophila melanogaster, GI19922690, Length=299, Percent_Identity=31.7725752508361, Blast_Score=127, Evalue=9e-30,
Organism=Drosophila melanogaster, GI45550900, Length=436, Percent_Identity=27.0642201834862, Blast_Score=123, Evalue=3e-28,
Organism=Drosophila melanogaster, GI17137380, Length=337, Percent_Identity=29.673590504451, Blast_Score=116, Evalue=2e-26,
Organism=Drosophila melanogaster, GI24647046, Length=269, Percent_Identity=26.3940520446097, Blast_Score=79, Evalue=4e-15,
Organism=Drosophila melanogaster, GI24647048, Length=269, Percent_Identity=26.3940520446097, Blast_Score=79, Evalue=4e-15,
Organism=Drosophila melanogaster, GI24582462, Length=148, Percent_Identity=31.7567567567568, Blast_Score=65, Evalue=7e-11,
Organism=Drosophila melanogaster, GI28572034, Length=159, Percent_Identity=35.8490566037736, Blast_Score=65, Evalue=9e-11,

Paralogues:

None

Copy number: 20568 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR009001
- InterPro:   IPR004161
- InterPro:   IPR004541
- InterPro:   IPR004160
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF03143 GTP_EFTU_D3 [H]

EC number: 3.6.5.3

Molecular weight: Translated: 42626; Mature: 42495

Theoretical pI: Translated: 5.02; Mature: 5.02

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKSKFERNKPHVNIGTIGHVDHGKTSLTAAITKYFGEFKAYDQIDAAPEEKARGITIST
CCCCCCCCCCCEEEECEEECCCCCCHHHHHHHHHHHHHHHCCHHCCCCCHHHHCCEEEEE
AHVEYETPARHYAHVDCPGHADYVKNMITGAAQMDGAILVCSAADGPMPQTREHILLARQ
EEEEECCCCCCEEEECCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHHHHHHH
VGVPAIVVFLNKVDQVDDAELLELVELEVRELLSSYDFPGDDIPIIKGSALAALEDSDKK
CCCCEEEEEHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEECCCEEEEECCCCHH
IGEDAIRELMAAVDAYIPTPERPIDQPFLMPIEDVFSISGRGTVVTGRVERGIVKVGEEV
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHCCCCCCEEEECHHHHCHHCCCCEE
EIVGIRPTSKTTVTGVEMFRKLLDQGQAGDNIGALVRGVTRDGVERGQILCKPGSVKPHK
EEEEECCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCEEEECCCCCCHHH
KFMAEAYILTKEEGGRHTPFFTNYRPQFYFRTTDVTGIVSLPEGTEMVMPGDNVTVEVEL
HHHHHEEEEEECCCCCCCCEEECCCCEEEEEECCCEEEEECCCCCEEEECCCCEEEEEEE
IVPIAMEEKLRFAIREGGRTVGAGIVASIVE
EEEECHHHHHHHHHHHCCCEEHHHHHHHHCC
>Mature Secondary Structure 
AKSKFERNKPHVNIGTIGHVDHGKTSLTAAITKYFGEFKAYDQIDAAPEEKARGITIST
CCCCCCCCCCEEEECEEECCCCCCHHHHHHHHHHHHHHHCCHHCCCCCHHHHCCEEEEE
AHVEYETPARHYAHVDCPGHADYVKNMITGAAQMDGAILVCSAADGPMPQTREHILLARQ
EEEEECCCCCCEEEECCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHHHHHHH
VGVPAIVVFLNKVDQVDDAELLELVELEVRELLSSYDFPGDDIPIIKGSALAALEDSDKK
CCCCEEEEEHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEECCCEEEEECCCCHH
IGEDAIRELMAAVDAYIPTPERPIDQPFLMPIEDVFSISGRGTVVTGRVERGIVKVGEEV
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHCCCCCCEEEECHHHHCHHCCCCEE
EIVGIRPTSKTTVTGVEMFRKLLDQGQAGDNIGALVRGVTRDGVERGQILCKPGSVKPHK
EEEEECCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCEEEECCCCCCHHH
KFMAEAYILTKEEGGRHTPFFTNYRPQFYFRTTDVTGIVSLPEGTEMVMPGDNVTVEVEL
HHHHHEEEEEECCCCCCCCEEECCCCEEEEEECCCEEEEECCCCCEEEECCCCEEEEEEE
IVPIAMEEKLRFAIREGGRTVGAGIVASIVE
EEEECHHHHHHHHHHHCCCEEHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GTP; H2O

Specific reaction: GTP + H2O = GDP + phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA