Click here to switch to the map view.

The map label for this gene is pcm [H]

Identifier: 15889027

GI number: 15889027

Start: 1709941

End: 1710612

Strand: Reverse

Name: pcm [H]

Synonym: Atu1721

Alternate gene names: 15889027

Gene position: 1710612-1709941 (Counterclockwise)

Preceding gene: 159184885

Following gene: 15889026

Centisome position: 60.2

GC content: 60.27

Gene sequence:

>672_bases
ATGATGGATTTCGAAACCGCACGCGCCAATATGGTCGACAGCCAGTTGCGCACGACCGACGTGACGTCGCATTCCGTGCT
GAAGGCGTTTTTGAGCGTGCCGCGCGAGGCATTCGTGCCGGCGGGTGTGCGGCAGATTGCCTATGCGGATGAGGATCTGC
AGATCTGTCCGGCGCTTGACGGTCGCCCGGCCCGTTATGTCATGAAGGCATCGCCGCTGGCCAAGCTTTTGCAGCTGGCT
GCCGTCTCCAAGGACGATGTGGTGCTGGAAGTCGGTGGTGGCTCCGGTTACGCGGCTGCCATCCTGTCCCAGCTTGCCGG
TTCGGTGGTTTCGCTTGAAAGCGACGAAGCGCTGGCCGCACAGGCGACCGAGACGCTCGCTTCGCTCGGTTATGACAATG
TCGCTGTCGTGACCGGCGATCTTGCCAAGGGTTATGCGAGTGAAGCGCCCTATGACCTGATTTTCATCAATGGTTCGGTC
GAGGAAGTGCCGGCGGCGCTGACCGACCAGTTGCGCGATGGCGGACGTCTTGTCGTCGTTGTCGGATATGGCAATGCTGC
CAAGGCAACTGTCTATCGCCGTGATGGCAACAGCACGTCTGCTGCGAGCTTTTTCAACGCCTCCGTCAAGCCTGTACCGG
GTTTTGCCAAGGCGGCCGAGTTCGTTTTCTGA

Upstream 100 bases:

>100_bases
GCGGCTTCTGGCAATTATGAGATTGTTTTAACGGTCGGCTGCATCTTCCGACTTCGGGCTGGTTTCCAGCCCCTATCCTT
GAAATTGAGGCGATAAGGAC

Downstream 100 bases:

>100_bases
TCCATACGCGATCGTTTTTTGCAGGGCCGGGGTGGATTTTTCACCCCGGCTTTTTTGTGGCCGGTGTCTCGGAAAATTGT
GTCGTCGCCTGCCGTTTTCA

Product: protein-L-isoaspartate O-methyltransferase

Products: NA

Alternate protein names: L-isoaspartyl protein carboxyl methyltransferase; Protein L-isoaspartyl methyltransferase; Protein-beta-aspartate methyltransferase; PIMT [H]

Number of amino acids: Translated: 223; Mature: 223

Protein sequence:

>223_residues
MMDFETARANMVDSQLRTTDVTSHSVLKAFLSVPREAFVPAGVRQIAYADEDLQICPALDGRPARYVMKASPLAKLLQLA
AVSKDDVVLEVGGGSGYAAAILSQLAGSVVSLESDEALAAQATETLASLGYDNVAVVTGDLAKGYASEAPYDLIFINGSV
EEVPAALTDQLRDGGRLVVVVGYGNAAKATVYRRDGNSTSAASFFNASVKPVPGFAKAAEFVF

Sequences:

>Translated_223_residues
MMDFETARANMVDSQLRTTDVTSHSVLKAFLSVPREAFVPAGVRQIAYADEDLQICPALDGRPARYVMKASPLAKLLQLA
AVSKDDVVLEVGGGSGYAAAILSQLAGSVVSLESDEALAAQATETLASLGYDNVAVVTGDLAKGYASEAPYDLIFINGSV
EEVPAALTDQLRDGGRLVVVVGYGNAAKATVYRRDGNSTSAASFFNASVKPVPGFAKAAEFVF
>Mature_223_residues
MMDFETARANMVDSQLRTTDVTSHSVLKAFLSVPREAFVPAGVRQIAYADEDLQICPALDGRPARYVMKASPLAKLLQLA
AVSKDDVVLEVGGGSGYAAAILSQLAGSVVSLESDEALAAQATETLASLGYDNVAVVTGDLAKGYASEAPYDLIFINGSV
EEVPAALTDQLRDGGRLVVVVGYGNAAKATVYRRDGNSTSAASFFNASVKPVPGFAKAAEFVF

Specific function: Catalyzes the methyl esterification of L-isoaspartyl residues in peptides and proteins that result from spontaneous decomposition of normal L-aspartyl and L-asparaginyl residues. It plays a role in the repair and/or degradation of damaged proteins [H]

COG id: COG2518

COG function: function code O; Protein-L-isoaspartate carboxylmethyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. L- isoaspartyl/D-aspartyl protein methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789100, Length=192, Percent_Identity=34.8958333333333, Blast_Score=102, Evalue=2e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000682 [H]

Pfam domain/function: PF01135 PCMT [H]

EC number: =2.1.1.77 [H]

Molecular weight: Translated: 23300; Mature: 23300

Theoretical pI: Translated: 4.43; Mature: 4.43

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMDFETARANMVDSQLRTTDVTSHSVLKAFLSVPREAFVPAGVRQIAYADEDLQICPALD
CCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHCCHHHHCCCCHHHHHCCCCCCEECCCCC
GRPARYVMKASPLAKLLQLAAVSKDDVVLEVGGGSGYAAAILSQLAGSVVSLESDEALAA
CCCCEEEEEHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHCCCEEECCCCCHHHH
QATETLASLGYDNVAVVTGDLAKGYASEAPYDLIFINGSVEEVPAALTDQLRDGGRLVVV
HHHHHHHHCCCCCEEEEECCHHHCCCCCCCEEEEEECCCHHHHHHHHHHHHCCCCEEEEE
VGYGNAAKATVYRRDGNSTSAASFFNASVKPVPGFAKAAEFVF
EECCCCCEEEEEEECCCCCCHHHHHCCCCCCCCCHHHHHHHCC
>Mature Secondary Structure
MMDFETARANMVDSQLRTTDVTSHSVLKAFLSVPREAFVPAGVRQIAYADEDLQICPALD
CCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHCCHHHHCCCCHHHHHCCCCCCEECCCCC
GRPARYVMKASPLAKLLQLAAVSKDDVVLEVGGGSGYAAAILSQLAGSVVSLESDEALAA
CCCCEEEEEHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHCCCEEECCCCCHHHH
QATETLASLGYDNVAVVTGDLAKGYASEAPYDLIFINGSVEEVPAALTDQLRDGGRLVVV
HHHHHHHHCCCCCEEEEECCHHHCCCCCCCEEEEEECCCHHHHHHHHHHHHCCCCEEEEE
VGYGNAAKATVYRRDGNSTSAASFFNASVKPVPGFAKAAEFVF
EECCCCCEEEEEEECCCCCCHHHHHCCCCCCCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA