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The map label for this gene is rnhA

Identifier: 15888119

GI number: 15888119

Start: 771839

End: 772279

Strand: Direct

Name: rnhA

Synonym: Atu0776

Alternate gene names: 15888119

Gene position: 771839-772279 (Clockwise)

Preceding gene: 15888118

Following gene: 15888120

Centisome position: 27.16

GC content: 61.0

Gene sequence:

>441_bases
ATGAAACACGTCGATATTTTCACCGATGGCGCCTGCTCCGGCAATCCCGGGCCGGGCGGCTGGGGTGCGGTGCTGCGTTA
TGGCGAGACCGAAAAGGAACTCTCCGGCGGCGAAGCCGACACCACCAACAACCGCATGGAATTGCTGGCGGCGATCTCGG
CGCTCAACGCGCTGAAGAGCCCTTGCGAGGTCGATCTTTATACCGACAGCGCCTATGTGAAGGACGGCATCACCAAGTGG
ATTTTCGGCTGGAAAAAGAAGGGCTGGAAAACCGCCGATAACAAGCCGGTCAAGAATGTCGAGCTTTGGCAGGCGCTGGA
GGCGGCGCAGGAGCGCCACAAGGTCACCCTGCATTGGGTGAAGGGCCATGCCGGCCACCCGGAAAACGAGCGTGCCGATG
AGCTGGCGCGCAAGGGCATGGAGCCTTTCAAACGCCGGTAA

Upstream 100 bases:

>100_bases
CCGGCGCGCTGGTGGTGAAGAAGGATCCGCTGGAATATCTGCGCAAGCTGCGCTTCCACCGCACGATCGCCAATGTCGCC
GAATATGGGCTGGCGGGCGA

Downstream 100 bases:

>100_bases
CGCAGGCCTGCTGTCTTGTCGGTGTGACTGGCTCTGGGCGGGCGCGACGCTCAATCCCGATTGGGGGTCCGATTGAGAGA
TCGATTTGCGGCCCGATTGG

Product: ribonuclease H

Products: NA

Alternate protein names: RNase H

Number of amino acids: Translated: 146; Mature: 146

Protein sequence:

>146_residues
MKHVDIFTDGACSGNPGPGGWGAVLRYGETEKELSGGEADTTNNRMELLAAISALNALKSPCEVDLYTDSAYVKDGITKW
IFGWKKKGWKTADNKPVKNVELWQALEAAQERHKVTLHWVKGHAGHPENERADELARKGMEPFKRR

Sequences:

>Translated_146_residues
MKHVDIFTDGACSGNPGPGGWGAVLRYGETEKELSGGEADTTNNRMELLAAISALNALKSPCEVDLYTDSAYVKDGITKW
IFGWKKKGWKTADNKPVKNVELWQALEAAQERHKVTLHWVKGHAGHPENERADELARKGMEPFKRR
>Mature_146_residues
MKHVDIFTDGACSGNPGPGGWGAVLRYGETEKELSGGEADTTNNRMELLAAISALNALKSPCEVDLYTDSAYVKDGITKW
IFGWKKKGWKTADNKPVKNVELWQALEAAQERHKVTLHWVKGHAGHPENERADELARKGMEPFKRR

Specific function: Endonuclease that specifically degrades the RNA of RNA- DNA hybrids

COG id: COG0328

COG function: function code L; Ribonuclease HI

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 RNase H domain

Homologues:

Organism=Homo sapiens, GI21359816, Length=147, Percent_Identity=40.1360544217687, Blast_Score=80, Evalue=7e-16,
Organism=Escherichia coli, GI1786408, Length=143, Percent_Identity=67.1328671328671, Blast_Score=198, Evalue=1e-52,
Organism=Caenorhabditis elegans, GI115532542, Length=146, Percent_Identity=37.6712328767123, Blast_Score=90, Evalue=4e-19,
Organism=Drosophila melanogaster, GI45552509, Length=154, Percent_Identity=34.4155844155844, Blast_Score=88, Evalue=3e-18,
Organism=Drosophila melanogaster, GI17137476, Length=154, Percent_Identity=34.4155844155844, Blast_Score=87, Evalue=3e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RNH_AGRT5 (Q8UHA7)

Other databases:

- EMBL:   AE007869
- PIR:   AB2672
- PIR:   H97453
- RefSeq:   NP_353800.1
- ProteinModelPortal:   Q8UHA7
- SMR:   Q8UHA7
- STRING:   Q8UHA7
- GeneID:   1132814
- GenomeReviews:   AE007869_GR
- KEGG:   atu:Atu0776
- eggNOG:   COG0328
- HOGENOM:   HBG742437
- OMA:   NGWRTAD
- PhylomeDB:   Q8UHA7
- ProtClustDB:   PRK00203
- BioCyc:   ATUM176299-1:ATU0776-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00042
- InterPro:   IPR022892
- InterPro:   IPR012337
- InterPro:   IPR002156

Pfam domain/function: PF00075 RnaseH; SSF53098 RNaseH_fold

EC number: =3.1.26.4

Molecular weight: Translated: 16261; Mature: 16261

Theoretical pI: Translated: 8.24; Mature: 8.24

Prosite motif: PS50879 RNASE_H

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKHVDIFTDGACSGNPGPGGWGAVLRYGETEKELSGGEADTTNNRMELLAAISALNALKS
CCCCCEEECCCCCCCCCCCCHHHHEECCCCHHHHCCCCCCCCCHHHHHHHHHHHHHHHCC
PCEVDLYTDSAYVKDGITKWIFGWKKKGWKTADNKPVKNVELWQALEAAQERHKVTLHWV
CCEEEEECCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHCEEEEEEE
KGHAGHPENERADELARKGMEPFKRR
CCCCCCCCCHHHHHHHHCCCCHHHCC
>Mature Secondary Structure
MKHVDIFTDGACSGNPGPGGWGAVLRYGETEKELSGGEADTTNNRMELLAAISALNALKS
CCCCCEEECCCCCCCCCCCCHHHHEECCCCHHHHCCCCCCCCCHHHHHHHHHHHHHHHCC
PCEVDLYTDSAYVKDGITKWIFGWKKKGWKTADNKPVKNVELWQALEAAQERHKVTLHWV
CCEEEEECCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHCEEEEEEE
KGHAGHPENERADELARKGMEPFKRR
CCCCCCCCCHHHHHHHHCCCCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11743193; 11743194