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The map label for this gene is truA

Identifier: 15887719

GI number: 15887719

Start: 362338

End: 363138

Strand: Direct

Name: truA

Synonym: Atu0368

Alternate gene names: 15887719

Gene position: 362338-363138 (Clockwise)

Preceding gene: 15887718

Following gene: 15887723

Centisome position: 12.75

GC content: 62.55

Gene sequence:

>801_bases
ATGCCACGTTTCCGCATGACCGTCGAATATGACGGCACGCCCTATTACGGCTGGCAGAGACAGGAAAACGGCCCCTCCGT
TCAGGGCGCGCTGGAGGCTGCCGTGCGGTCGCTGACCGGGGAGAGCGTTTCCATTCGTGGCGCAGGCCGCACAGATTCCG
GTGTGCATGCCGTGGGACAGGTCGCCCATATCGATCTTTCCCGCGATTGGGAACCCTACAAGCTCCGCAATGCCTTGAAC
GCACACCTGGCCATGGCGGGTGAGGCCGTGGCCGTGCTCGATGCCGCCGCGGTGACCGAGGATTTCGACGCGCGTTTTTC
AGCGCTGCGCCGCCATTATCTCTACCGCATCATTTGTCGCAAATCCCGGCTGGCGCTGGAGCACAAGCGGGCATGGTGGG
TATCGAAGGACTTGGACCACGAGCGCATGCATGAAGCCGCGCAGATGCTCGTCGGCCGGCATGATTTCACCACCTTCCGC
TCTGTCCATTGTCAGGCGAACAGCCCGGTCCGCACGCTCGACCGGCTGGATGTGACGCGCAACGGCGATCTCATCGAGAT
ACGCGCCACCGCGCAGAGTTTTCTGCACAACCAAATCCGTTCTTTTGCCGGGACGTTGAAATTGGCGGGCGAAGGCGGCA
TGACCCCGGACGATGTGCGCGCCGCATTGGAGGCGCGGGATCGCAAGGCCTGCGGCCCCGTTGCACCGCCGGACGGTCTT
TATTTCATGCAGGTCGATTATCCCGATGTCATTCCGCCGCGCCGCCGCCCTGAAATTGAAATGACGGAAGACGCGGACTG
A

Upstream 100 bases:

>100_bases
CCGTGCGGCTCACACGCTTGCAGAAGGCGGGCGGCAAGCCCATGTCGGCGGCCGATTTCGTGCGTGGTACGCCGGTTCCA
GCCACAACGAGGCTCGGCTG

Downstream 100 bases:

>100_bases
TCGAAGGCCCCGCCGCCTCTCAGGCGGGATAGACGCCGAGAAAACGCTGCAATGCCTCTGACAGCAACATGGCGGGCACC
AGCAGCGTCATGACCACTGC

Product: tRNA pseudouridine synthase A

Products: NA

Alternate protein names: tRNA pseudouridylate synthase I; tRNA-uridine isomerase I

Number of amino acids: Translated: 266; Mature: 265

Protein sequence:

>266_residues
MPRFRMTVEYDGTPYYGWQRQENGPSVQGALEAAVRSLTGESVSIRGAGRTDSGVHAVGQVAHIDLSRDWEPYKLRNALN
AHLAMAGEAVAVLDAAAVTEDFDARFSALRRHYLYRIICRKSRLALEHKRAWWVSKDLDHERMHEAAQMLVGRHDFTTFR
SVHCQANSPVRTLDRLDVTRNGDLIEIRATAQSFLHNQIRSFAGTLKLAGEGGMTPDDVRAALEARDRKACGPVAPPDGL
YFMQVDYPDVIPPRRRPEIEMTEDAD

Sequences:

>Translated_266_residues
MPRFRMTVEYDGTPYYGWQRQENGPSVQGALEAAVRSLTGESVSIRGAGRTDSGVHAVGQVAHIDLSRDWEPYKLRNALN
AHLAMAGEAVAVLDAAAVTEDFDARFSALRRHYLYRIICRKSRLALEHKRAWWVSKDLDHERMHEAAQMLVGRHDFTTFR
SVHCQANSPVRTLDRLDVTRNGDLIEIRATAQSFLHNQIRSFAGTLKLAGEGGMTPDDVRAALEARDRKACGPVAPPDGL
YFMQVDYPDVIPPRRRPEIEMTEDAD
>Mature_265_residues
PRFRMTVEYDGTPYYGWQRQENGPSVQGALEAAVRSLTGESVSIRGAGRTDSGVHAVGQVAHIDLSRDWEPYKLRNALNA
HLAMAGEAVAVLDAAAVTEDFDARFSALRRHYLYRIICRKSRLALEHKRAWWVSKDLDHERMHEAAQMLVGRHDFTTFRS
VHCQANSPVRTLDRLDVTRNGDLIEIRATAQSFLHNQIRSFAGTLKLAGEGGMTPDDVRAALEARDRKACGPVAPPDGLY
FMQVDYPDVIPPRRRPEIEMTEDAD

Specific function: Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs

COG id: COG0101

COG function: function code J; Pseudouridylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA pseudouridine synthase truA family

Homologues:

Organism=Homo sapiens, GI23503259, Length=277, Percent_Identity=34.6570397111913, Blast_Score=119, Evalue=2e-27,
Organism=Homo sapiens, GI199559785, Length=282, Percent_Identity=27.3049645390071, Blast_Score=69, Evalue=6e-12,
Organism=Escherichia coli, GI1788657, Length=247, Percent_Identity=38.8663967611336, Blast_Score=172, Evalue=2e-44,
Organism=Drosophila melanogaster, GI116007752, Length=304, Percent_Identity=28.9473684210526, Blast_Score=92, Evalue=3e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRUA_AGRT5 (Q8UIC9)

Other databases:

- EMBL:   AE007869
- PIR:   AH2621
- PIR:   H97403
- RefSeq:   NP_353400.1
- ProteinModelPortal:   Q8UIC9
- SMR:   Q8UIC9
- STRING:   Q8UIC9
- GeneID:   1132406
- GenomeReviews:   AE007869_GR
- KEGG:   atu:Atu0368
- eggNOG:   COG0101
- HOGENOM:   HBG621020
- OMA:   HSAIRRH
- PhylomeDB:   Q8UIC9
- ProtClustDB:   PRK00021
- BioCyc:   ATUM176299-1:ATU0368-MONOMER
- HAMAP:   MF_00171
- InterPro:   IPR020103
- InterPro:   IPR001406
- InterPro:   IPR020097
- InterPro:   IPR020095
- InterPro:   IPR020094
- Gene3D:   G3DSA:3.30.70.580
- Gene3D:   G3DSA:3.30.70.660
- PANTHER:   PTHR11142
- PIRSF:   PIRSF001430
- TIGRFAMs:   TIGR00071

Pfam domain/function: PF01416 PseudoU_synth_1; SSF55120 PsdUridine_synth_cat_dom

EC number: =5.4.99.12

Molecular weight: Translated: 29884; Mature: 29753

Theoretical pI: Translated: 7.07; Mature: 7.07

Prosite motif: NA

Important sites: ACT_SITE 52-52

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPRFRMTVEYDGTPYYGWQRQENGPSVQGALEAAVRSLTGESVSIRGAGRTDSGVHAVGQ
CCCEEEEEEECCCCCCCCEECCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHH
VAHIDLSRDWEPYKLRNALNAHLAMAGEAVAVLDAAAVTEDFDARFSALRRHYLYRIICR
EEEEECCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KSRLALEHKRAWWVSKDLDHERMHEAAQMLVGRHDFTTFRSVHCQANSPVRTLDRLDVTR
HHHHHHHHHHHEECCCCCCHHHHHHHHHHHHCCCCCHHHHEEEECCCCCHHHHHHHCCCC
NGDLIEIRATAQSFLHNQIRSFAGTLKLAGEGGMTPDDVRAALEARDRKACGPVAPPDGL
CCCEEEEHHHHHHHHHHHHHHHHCEEEECCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCE
YFMQVDYPDVIPPRRRPEIEMTEDAD
EEEEECCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
PRFRMTVEYDGTPYYGWQRQENGPSVQGALEAAVRSLTGESVSIRGAGRTDSGVHAVGQ
CCEEEEEEECCCCCCCCEECCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHH
VAHIDLSRDWEPYKLRNALNAHLAMAGEAVAVLDAAAVTEDFDARFSALRRHYLYRIICR
EEEEECCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KSRLALEHKRAWWVSKDLDHERMHEAAQMLVGRHDFTTFRSVHCQANSPVRTLDRLDVTR
HHHHHHHHHHHEECCCCCCHHHHHHHHHHHHCCCCCHHHHEEEECCCCCHHHHHHHCCCC
NGDLIEIRATAQSFLHNQIRSFAGTLKLAGEGGMTPDDVRAALEARDRKACGPVAPPDGL
CCCEEEEHHHHHHHHHHHHHHHHCEEEECCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCE
YFMQVDYPDVIPPRRRPEIEMTEDAD
EEEEECCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11743193; 11743194