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The map label for this gene is yqiG [H]

Identifier: 57652581

GI number: 57652581

Start: 399550

End: 400749

Strand: Reverse

Name: yqiG [H]

Synonym: SACOL0392

Alternate gene names: 57652581

Gene position: 400749-399550 (Counterclockwise)

Preceding gene: 57652589

Following gene: 57652580

Centisome position: 14.26

GC content: 39.42

Gene sequence:

>1200_bases
ATGTACCGATACAAACCACTTTTACAATCAATCCACTTACCTAATGGCATCAAAATTTCGAATCGCTTTGTATTATCACC
AATGACCGTAAACACATCAACAAAAGAAGGCTATATTACAAAAGAAGACTTGGCTTATGCTGCACGTCGCTCTAATTCAG
CTGGAATGCAAGTCACCGGCGCTGCTTACATTGAACCTTATGGTCAATTGTTTGAATATGGCTTTAATATTGATCATGAT
GCTTGTATCCCTGGATTAACTAACATGGCATCTACGATGAAACAACATGGTAGCCTCGCTATTATCCAGCTAGCGCATGC
TGGTCGTTTTTCAAATCAAGCCATTTTAAATTTTGGAAAGGTGTATGGACCAAGCCCTATGACATTGCATTCACCGATTG
AACACGATGTTATCGCCATGTCTCATGAGAAAATCAATAGCATCATTCAGCAGTATCGCGATGCTACATTACGCGCGATT
AAAGCAGGTTTTGATGGTGTGGAAATTTCGATTGCACAACGTTTGCTGATTCAAACATTTTTCTCAACTTTTTCGAATAA
ACGTACAGACCATTACGGTGCCGACTCATTAAAAAATCGTGCAAGATTATGTTTAGAAGTTATGCGTGCGGTACAAGAAG
TGATTGATAAAGAAGCACCTGACAATTTTATTTTAGGATTTCGAGCAACGCCAGAAGAAACCAGAGGCAGTGATTTAGGA
TATACCATTGACGAGTTCAACCAATTAATCAATTGGGTCATGGACGTTTCAAACATCCAATACTTAGCAATCGCTAGCTG
GGGTCGACATATTTATCAAAATACGTCTCGTACACCAGGTGATCATTTCGGTCGACCAGTTAACCAAATTGTCTATGAAC
ATTTAGCAGGTCGTATCCCTTTAATTGCAAGTGGCGGGATTAATTCTCCAGAGTCTGCTTTAGATGCTTTACAACATGCA
GATATGGTCGGCATGTCTTCGCCTTTTGTCACAGAACCTGACTTTGTGCATAAACTTGCTGAACAGCGACCACATGATAT
CAATCTTGAATTTTCTATGGCAGATTTAGAAGATTTAGCCATTCCACATGCTGCTTTTAAAGATATTGTTAAAATGATGG
ATTATGGGGAAGGTCTAAAGAAGCATACCCGTGACGCTTTACGTCAATTGGAACAGAATTATCATGACTCATCGTCTTAA

Upstream 100 bases:

>100_bases
TCCATGTACCAATTCTATTCTATCAAGACTGTTCATCATTGATTATTCATTCTTTCCATGGTACTTTGAAAACAATATTG
AATAGAAAAGGAATCATAGT

Downstream 100 bases:

>100_bases
GACTATTTTTCATGGAAATTTAAGACAGTGTTATAATGACATCAATATCAAAAATAAGGAGCCTATTAAATCGTGACTGA
AATTAAAGATAAAGTAATTA

Product: Oye family NADH-dependent flavin oxidoreductase

Products: trans-transtetradehydroacyl-CoA; NADPH [C]

Alternate protein names: NA

Number of amino acids: Translated: 399; Mature: 399

Protein sequence:

>399_residues
MYRYKPLLQSIHLPNGIKISNRFVLSPMTVNTSTKEGYITKEDLAYAARRSNSAGMQVTGAAYIEPYGQLFEYGFNIDHD
ACIPGLTNMASTMKQHGSLAIIQLAHAGRFSNQAILNFGKVYGPSPMTLHSPIEHDVIAMSHEKINSIIQQYRDATLRAI
KAGFDGVEISIAQRLLIQTFFSTFSNKRTDHYGADSLKNRARLCLEVMRAVQEVIDKEAPDNFILGFRATPEETRGSDLG
YTIDEFNQLINWVMDVSNIQYLAIASWGRHIYQNTSRTPGDHFGRPVNQIVYEHLAGRIPLIASGGINSPESALDALQHA
DMVGMSSPFVTEPDFVHKLAEQRPHDINLEFSMADLEDLAIPHAAFKDIVKMMDYGEGLKKHTRDALRQLEQNYHDSSS

Sequences:

>Translated_399_residues
MYRYKPLLQSIHLPNGIKISNRFVLSPMTVNTSTKEGYITKEDLAYAARRSNSAGMQVTGAAYIEPYGQLFEYGFNIDHD
ACIPGLTNMASTMKQHGSLAIIQLAHAGRFSNQAILNFGKVYGPSPMTLHSPIEHDVIAMSHEKINSIIQQYRDATLRAI
KAGFDGVEISIAQRLLIQTFFSTFSNKRTDHYGADSLKNRARLCLEVMRAVQEVIDKEAPDNFILGFRATPEETRGSDLG
YTIDEFNQLINWVMDVSNIQYLAIASWGRHIYQNTSRTPGDHFGRPVNQIVYEHLAGRIPLIASGGINSPESALDALQHA
DMVGMSSPFVTEPDFVHKLAEQRPHDINLEFSMADLEDLAIPHAAFKDIVKMMDYGEGLKKHTRDALRQLEQNYHDSSS
>Mature_399_residues
MYRYKPLLQSIHLPNGIKISNRFVLSPMTVNTSTKEGYITKEDLAYAARRSNSAGMQVTGAAYIEPYGQLFEYGFNIDHD
ACIPGLTNMASTMKQHGSLAIIQLAHAGRFSNQAILNFGKVYGPSPMTLHSPIEHDVIAMSHEKINSIIQQYRDATLRAI
KAGFDGVEISIAQRLLIQTFFSTFSNKRTDHYGADSLKNRARLCLEVMRAVQEVIDKEAPDNFILGFRATPEETRGSDLG
YTIDEFNQLINWVMDVSNIQYLAIASWGRHIYQNTSRTPGDHFGRPVNQIVYEHLAGRIPLIASGGINSPESALDALQHA
DMVGMSSPFVTEPDFVHKLAEQRPHDINLEFSMADLEDLAIPHAAFKDIVKMMDYGEGLKKHTRDALRQLEQNYHDSSS

Specific function: Catalyzes The NADP-Dependent Reduction Of 2,4-Dienoyl- CoA To Yield Trans-2- Enoyl-CoA. [C]

COG id: COG1902

COG function: function code C; NADH:flavin oxidoreductases, Old Yellow Enzyme family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NADH:flavin oxidoreductase/NADH oxidase family [H]

Homologues:

Organism=Escherichia coli, GI1789463, Length=337, Percent_Identity=27.893175074184, Blast_Score=98, Evalue=1e-21,
Organism=Escherichia coli, GI1787939, Length=349, Percent_Identity=26.0744985673352, Blast_Score=80, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17540738, Length=429, Percent_Identity=25.1748251748252, Blast_Score=86, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI17565138, Length=373, Percent_Identity=24.6648793565684, Blast_Score=84, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17559802, Length=366, Percent_Identity=25.1366120218579, Blast_Score=80, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17559804, Length=370, Percent_Identity=25.1351351351351, Blast_Score=79, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI17564188, Length=368, Percent_Identity=22.8260869565217, Blast_Score=69, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI17566914, Length=243, Percent_Identity=26.3374485596708, Blast_Score=65, Evalue=9e-11,
Organism=Saccharomyces cerevisiae, GI6325086, Length=362, Percent_Identity=25.414364640884, Blast_Score=79, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6321973, Length=247, Percent_Identity=27.9352226720648, Blast_Score=72, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR001155 [H]

Pfam domain/function: PF00724 Oxidored_FMN [H]

EC number: 1.3.1.34 [C]

Molecular weight: Translated: 44692; Mature: 44692

Theoretical pI: Translated: 6.56; Mature: 6.56

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYRYKPLLQSIHLPNGIKISNRFVLSPMTVNTSTKEGYITKEDLAYAARRSNSAGMQVTG
CCCCHHHHHHCCCCCCCEECCEEEECCEEECCCCCCCCCCHHHHHHHHHCCCCCCCEEEC
AAYIEPYGQLFEYGFNIDHDACIPGLTNMASTMKQHGSLAIIQLAHAGRFSNQAILNFGK
HHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCEEEEEEECCCCCCCHHHHHHHH
VYGPSPMTLHSPIEHDVIAMSHEKINSIIQQYRDATLRAIKAGFDGVEISIAQRLLIQTF
HCCCCCCEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
FSTFSNKRTDHYGADSLKNRARLCLEVMRAVQEVIDKEAPDNFILGFRATPEETRGSDLG
HHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCC
YTIDEFNQLINWVMDVSNIQYLAIASWGRHIYQNTSRTPGDHFGRPVNQIVYEHLAGRIP
CCHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHCCCCCCCHHHCCCHHHHHHHHHCCCCC
LIASGGINSPESALDALQHADMVGMSSPFVTEPDFVHKLAEQRPHDINLEFSMADLEDLA
EEECCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCCEEEEECCCHHHHH
IPHAAFKDIVKMMDYGEGLKKHTRDALRQLEQNYHDSSS
CCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MYRYKPLLQSIHLPNGIKISNRFVLSPMTVNTSTKEGYITKEDLAYAARRSNSAGMQVTG
CCCCHHHHHHCCCCCCCEECCEEEECCEEECCCCCCCCCCHHHHHHHHHCCCCCCCEEEC
AAYIEPYGQLFEYGFNIDHDACIPGLTNMASTMKQHGSLAIIQLAHAGRFSNQAILNFGK
HHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCEEEEEEECCCCCCCHHHHHHHH
VYGPSPMTLHSPIEHDVIAMSHEKINSIIQQYRDATLRAIKAGFDGVEISIAQRLLIQTF
HCCCCCCEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
FSTFSNKRTDHYGADSLKNRARLCLEVMRAVQEVIDKEAPDNFILGFRATPEETRGSDLG
HHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCC
YTIDEFNQLINWVMDVSNIQYLAIASWGRHIYQNTSRTPGDHFGRPVNQIVYEHLAGRIP
CCHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHCCCCCCCHHHCCCHHHHHHHHHCCCCC
LIASGGINSPESALDALQHADMVGMSSPFVTEPDFVHKLAEQRPHDINLEFSMADLEDLA
EEECCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCCEEEEECCCHHHHH
IPHAAFKDIVKMMDYGEGLKKHTRDALRQLEQNYHDSSS
CCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: FAD. [C]

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: transDidehydroacyl-CoA; NADP+ [C]

Specific reaction: transDidehydroacyl-CoA + NADP+ =trans-transtetradehydroacyl-CoA + NADPH [C]

General reaction: Redox reaction [C]

Inhibitor: Iodoacetic acid; N-Ethyl maleimide [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969508; 9384377 [H]