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The map label for this gene is nikB [H]

Identifier: 57652283

GI number: 57652283

Start: 2531244

End: 2532179

Strand: Reverse

Name: nikB [H]

Synonym: SACOL2475

Alternate gene names: 57652283

Gene position: 2532179-2531244 (Counterclockwise)

Preceding gene: 57652284

Following gene: 57652282

Centisome position: 90.13

GC content: 33.87

Gene sequence:

>936_bases
ATGTTCAAATTTATCTTAAAACGTATTGCGCTCATGTTTCCATTGATGATTGTAGTAAGTTTTATGACATTTCTATTGAC
GTATATTACAAATGAAAATCCAGCTGTGACAATTTTACATGCACAAAGGACGCCAAATGTAACACCAGAGTTGATTGCAG
AAACGAATGAGAAGTACGGTTTCAATGATCCATTATTAATTCAATATAAAAATTGGTTACTTGAAGCGATGCAATTTAAT
TTTGGTACAAGCTACATTACAGGTGACCCAGTTGCTGAACGTATTGGTCCAGCATTTATGAATACATTGAAATTAACAAT
AATTTCAAGTGTTATGGTGATGATTACATCAATTATTTTAGGTGTAGTTAGTGCATTAAAAAGAGGAAAGTTCACTGATC
GTGCGATACGTTCAGTGGCTTTCGTTCTAACTGCATTACCATCATATTGGATAGCTTCAATACTTATTATTTACGTTTCA
GTGAAGTTAAACATATTGCCGACTTCTGGATTAACAGGTCCAGAAAGTTACATATTGCCAGTGATCGTTATTACGATTGC
CTATGCTGGTATTTACTTTAGAAATGTTAGACGCTCGATGGTGGAACAATTAAATGAAGATTATGTACTTTATTTAAGAG
CAAGCGGTGTGAAATCTATCACATTAATGTTGCATGTGTTGCGTAATGCTTTACAAGTTGCGGTATCAATCTTTTGTATG
TCTATACCAATGATAATGGGTGGACTAGTTGTTATCGAGTATATCTTTGCATGGCCTGGACTAGGTCAATTAAGTTTAAA
AGCAATACTTGAACACGATTTTCCAGTCATTCAAGCATATGTATTAATTGTAGCGGTATTATTTATTGTATTTAATACAT
TAGCAGATATCATTAATGCGCTATTAAATCCAAGATTAAGGGAGGGCGCACGATGA

Upstream 100 bases:

>100_bases
GACAGTTGTTGCACCAAAAGATTTAGAAAAAGTATCATTCACACAATCACAGTATGAATTACCATTCAATGAAATGCAGT
ATAAATAAAGGAGCAATTAG

Downstream 100 bases:

>100_bases
TAATTTTAAAACGATTATTACAAGATAAAGGTGCAGTAATTGCTTTAGGCATTATTGTATTATATGTCTTTTTAGGATTA
GCAGCACCACTTGTGACATT

Product: peptide ABC transporter permease

Products: Ni2; [Cytoplasm]; ADP; phosphate [C]

Alternate protein names: NA

Number of amino acids: Translated: 311; Mature: 311

Protein sequence:

>311_residues
MFKFILKRIALMFPLMIVVSFMTFLLTYITNENPAVTILHAQRTPNVTPELIAETNEKYGFNDPLLIQYKNWLLEAMQFN
FGTSYITGDPVAERIGPAFMNTLKLTIISSVMVMITSIILGVVSALKRGKFTDRAIRSVAFVLTALPSYWIASILIIYVS
VKLNILPTSGLTGPESYILPVIVITIAYAGIYFRNVRRSMVEQLNEDYVLYLRASGVKSITLMLHVLRNALQVAVSIFCM
SIPMIMGGLVVIEYIFAWPGLGQLSLKAILEHDFPVIQAYVLIVAVLFIVFNTLADIINALLNPRLREGAR

Sequences:

>Translated_311_residues
MFKFILKRIALMFPLMIVVSFMTFLLTYITNENPAVTILHAQRTPNVTPELIAETNEKYGFNDPLLIQYKNWLLEAMQFN
FGTSYITGDPVAERIGPAFMNTLKLTIISSVMVMITSIILGVVSALKRGKFTDRAIRSVAFVLTALPSYWIASILIIYVS
VKLNILPTSGLTGPESYILPVIVITIAYAGIYFRNVRRSMVEQLNEDYVLYLRASGVKSITLMLHVLRNALQVAVSIFCM
SIPMIMGGLVVIEYIFAWPGLGQLSLKAILEHDFPVIQAYVLIVAVLFIVFNTLADIINALLNPRLREGAR
>Mature_311_residues
MFKFILKRIALMFPLMIVVSFMTFLLTYITNENPAVTILHAQRTPNVTPELIAETNEKYGFNDPLLIQYKNWLLEAMQFN
FGTSYITGDPVAERIGPAFMNTLKLTIISSVMVMITSIILGVVSALKRGKFTDRAIRSVAFVLTALPSYWIASILIIYVS
VKLNILPTSGLTGPESYILPVIVITIAYAGIYFRNVRRSMVEQLNEDYVLYLRASGVKSITLMLHVLRNALQVAVSIFCM
SIPMIMGGLVVIEYIFAWPGLGQLSLKAILEHDFPVIQAYVLIVAVLFIVFNTLADIINALLNPRLREGAR

Specific function: Involved in a nickel transport system, probably translocates nickel through the bacterial inner membrane [H]

COG id: COG0601

COG function: function code EP; ABC-type dipeptide/oligopeptide/nickel transport systems, permease components

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1789888, Length=314, Percent_Identity=34.7133757961783, Blast_Score=203, Evalue=1e-53,
Organism=Escherichia coli, GI1787053, Length=308, Percent_Identity=33.4415584415584, Blast_Score=182, Evalue=2e-47,
Organism=Escherichia coli, GI1789965, Length=340, Percent_Identity=30.5882352941176, Blast_Score=170, Evalue=1e-43,
Organism=Escherichia coli, GI1787497, Length=311, Percent_Identity=28.6173633440514, Blast_Score=136, Evalue=1e-33,
Organism=Escherichia coli, GI1787761, Length=322, Percent_Identity=26.0869565217391, Blast_Score=120, Evalue=1e-28,
Organism=Escherichia coli, GI1787550, Length=265, Percent_Identity=27.1698113207547, Blast_Score=119, Evalue=2e-28,
Organism=Escherichia coli, GI1788504, Length=287, Percent_Identity=26.8292682926829, Blast_Score=98, Evalue=5e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515
- InterPro:   IPR014156 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 34832; Mature: 34832

Theoretical pI: Translated: 9.66; Mature: 9.66

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFKFILKRIALMFPLMIVVSFMTFLLTYITNENPAVTILHAQRTPNVTPELIAETNEKYG
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCHHHHHCCCCCCC
FNDPLLIQYKNWLLEAMQFNFGTSYITGDPVAERIGPAFMNTLKLTIISSVMVMITSIIL
CCCCEEHHHHHHHHHHHHHCCCCCCCCCCHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHH
GVVSALKRGKFTDRAIRSVAFVLTALPSYWIASILIIYVSVKLNILPTSGLTGPESYILP
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCHHHHHH
VIVITIAYAGIYFRNVRRSMVEQLNEDYVLYLRASGVKSITLMLHVLRNALQVAVSIFCM
HHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHH
SIPMIMGGLVVIEYIFAWPGLGQLSLKAILEHDFPVIQAYVLIVAVLFIVFNTLADIINA
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
LLNPRLREGAR
HHCCHHHCCCC
>Mature Secondary Structure
MFKFILKRIALMFPLMIVVSFMTFLLTYITNENPAVTILHAQRTPNVTPELIAETNEKYG
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCHHHHHCCCCCCC
FNDPLLIQYKNWLLEAMQFNFGTSYITGDPVAERIGPAFMNTLKLTIISSVMVMITSIIL
CCCCEEHHHHHHHHHHHHHCCCCCCCCCCHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHH
GVVSALKRGKFTDRAIRSVAFVLTALPSYWIASILIIYVSVKLNILPTSGLTGPESYILP
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCHHHHHH
VIVITIAYAGIYFRNVRRSMVEQLNEDYVLYLRASGVKSITLMLHVLRNALQVAVSIFCM
HHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHH
SIPMIMGGLVVIEYIFAWPGLGQLSLKAILEHDFPVIQAYVLIVAVLFIVFNTLADIINA
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
LLNPRLREGAR
HHCCHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Ni2; [Periplasm]; ATP; H2O [C]

Specific reaction: Ni2+ [Periplasm] + ATP + H2O = Ni2+ [Cytoplasm] + ADP + phosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7934931; 8041620; 9278503 [H]