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The map label for this gene is opuD2 [H]

Identifier: 57652202

GI number: 57652202

Start: 2256981

End: 2258543

Strand: Reverse

Name: opuD2 [H]

Synonym: SACOL2176

Alternate gene names: 57652202

Gene position: 2258543-2256981 (Counterclockwise)

Preceding gene: 57652203

Following gene: 57652201

Centisome position: 80.39

GC content: 35.89

Gene sequence:

>1563_bases
ATGAAAAATAAATCAGTAAGTCTAGTGTTTTGGGTATCTTTAGTGATATGTACGATATTTGTTGCTTTTGGAGCAATATT
TCCGAAACAGTTAGAAAAACTCACGCAAAACATTACTACATTTATTGCGCTACATTTTTCTTGGTATTATTTATTGCTCG
TTTTAGTTATTTTATTTGTCTGTGTGTATATTTTATTTTCGAGATACGCAAACATTACATTAGGTGAAGAAGGCGAAGAT
CCTGAATTCTCATTGCCGTCTTGGTTTGCTATGTTATTTAGTGCTGGTATGGGAATAGGACTTGTGTTCTGGACAACGGC
AGAACCAATCAGTCATGCCTTCAAATTAACGCCTATTCATAAAGCTGGCACGCAATCTGCAATTAATGATGCAATGCAGT
TTTCATTTTTCCATTGGGGGATTCATGCATGGGCAGTCTATGGTATTGTCGCGCTAGTGTTTGCATATTTTAGCTTTCAT
AAAGGATATCCTGGCTTAGTCAGCGCTACATTGACGCCTTTATTAGGTGAAAAGGCAATGCGTGGACCTTTAGGTGGTGC
CATTGATGTATTGGCAGTCATAGCTACAGTAACAGGCGTTGCTGCAACATTAGGTTTCGGTGCATTGCAAATAAACGAAG
GTTTACATTTTTTATTTAATGTACCGAGTAACTTCACGATGCAAGTAATCTTGATTGTCATTGCAACCATATTATTTACT
TGGTCAGCATGGTCAGGTATCGATAAAGGTATCAAAACGTTGAGTAATATCAACATGTTACTTGCTTTTGTAGTACTTAT
TGGTCTTTTTATTGTTGGGCCAACGCTATACATTTTAAATACATTTACGAATGGTCTAGGTAATTATATTGCAAATTTCT
TTAGTATGAGTTTACGCATTCCGACAGGTGGTCAAAAATTCCAATGGTTACAAAATTGGACAATCTTTTACTGGGCATGG
TGGATATCTTGGGCACCATTTGTAGGTATATTTATTGCACGTGTGTCAAAAGGACGTACGATTAAAGAATTTATTCTAGG
TGTACTTTTTGTCCCGGCGTTAGTTTGCTTTATATTCTTTGCGGTATTCGGCGCGTCAGCAATTTACTTACAAGATAATC
ATATTGCCGATATTGCTAAAGCTGCGACAGAAACAGCAACTTTTGCGACGCTACAACATTATCCGCTAGGTTTTGTATTA
AGCTTAATTACTTTACTTGTTATTATGATTTTCTTTGTTACATCAGCGGATTCGGCAACTTATGTTTTAGGTATGTTGAG
TTCAAGTGGTGATATTAATCCGAAGTCATTTGTCAAAGTGAGTTGGGGTATCATTATGGCATTATTTGCAATCATCATGA
TTTACACAGGTGGTACTCAAGCAATTCAAAATTTATTAATCATTGCTGCGTTACCATTTTCCGTGGTCATTATAGCGATG
ATATGGTCGCTTATAAAATCTTTGAGTGAGGAGAAACCGAGAAATAGTAATAAGGTTTTAATTAAACATCGTGACCCAGA
TATTTTAGAATATCGTTCTTCAAAACATATTGACGAAAATTAA

Upstream 100 bases:

>100_bases
AGATGAGGCATTATTAATTGTGTCTATATAAAATTACAAATGATTAAAATGTTGCAGTTATGGGTATGAACTTACCATTA
CACAGAAAGGGTTTGTATTT

Downstream 100 bases:

>100_bases
TTAATAATGAGGTTTTATTTACAAAAACTAGGGAATATATTACTAAATGGTACTTCATCTTATATATTAAAAATAAATTT
AAAGTTATTAATTTATTATG

Product: BCCT family osmoprotectant transporter

Products: Proton [Cytoplasm]; choline [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 520; Mature: 520

Protein sequence:

>520_residues
MKNKSVSLVFWVSLVICTIFVAFGAIFPKQLEKLTQNITTFIALHFSWYYLLLVLVILFVCVYILFSRYANITLGEEGED
PEFSLPSWFAMLFSAGMGIGLVFWTTAEPISHAFKLTPIHKAGTQSAINDAMQFSFFHWGIHAWAVYGIVALVFAYFSFH
KGYPGLVSATLTPLLGEKAMRGPLGGAIDVLAVIATVTGVAATLGFGALQINEGLHFLFNVPSNFTMQVILIVIATILFT
WSAWSGIDKGIKTLSNINMLLAFVVLIGLFIVGPTLYILNTFTNGLGNYIANFFSMSLRIPTGGQKFQWLQNWTIFYWAW
WISWAPFVGIFIARVSKGRTIKEFILGVLFVPALVCFIFFAVFGASAIYLQDNHIADIAKAATETATFATLQHYPLGFVL
SLITLLVIMIFFVTSADSATYVLGMLSSSGDINPKSFVKVSWGIIMALFAIIMIYTGGTQAIQNLLIIAALPFSVVIIAM
IWSLIKSLSEEKPRNSNKVLIKHRDPDILEYRSSKHIDEN

Sequences:

>Translated_520_residues
MKNKSVSLVFWVSLVICTIFVAFGAIFPKQLEKLTQNITTFIALHFSWYYLLLVLVILFVCVYILFSRYANITLGEEGED
PEFSLPSWFAMLFSAGMGIGLVFWTTAEPISHAFKLTPIHKAGTQSAINDAMQFSFFHWGIHAWAVYGIVALVFAYFSFH
KGYPGLVSATLTPLLGEKAMRGPLGGAIDVLAVIATVTGVAATLGFGALQINEGLHFLFNVPSNFTMQVILIVIATILFT
WSAWSGIDKGIKTLSNINMLLAFVVLIGLFIVGPTLYILNTFTNGLGNYIANFFSMSLRIPTGGQKFQWLQNWTIFYWAW
WISWAPFVGIFIARVSKGRTIKEFILGVLFVPALVCFIFFAVFGASAIYLQDNHIADIAKAATETATFATLQHYPLGFVL
SLITLLVIMIFFVTSADSATYVLGMLSSSGDINPKSFVKVSWGIIMALFAIIMIYTGGTQAIQNLLIIAALPFSVVIIAM
IWSLIKSLSEEKPRNSNKVLIKHRDPDILEYRSSKHIDEN
>Mature_520_residues
MKNKSVSLVFWVSLVICTIFVAFGAIFPKQLEKLTQNITTFIALHFSWYYLLLVLVILFVCVYILFSRYANITLGEEGED
PEFSLPSWFAMLFSAGMGIGLVFWTTAEPISHAFKLTPIHKAGTQSAINDAMQFSFFHWGIHAWAVYGIVALVFAYFSFH
KGYPGLVSATLTPLLGEKAMRGPLGGAIDVLAVIATVTGVAATLGFGALQINEGLHFLFNVPSNFTMQVILIVIATILFT
WSAWSGIDKGIKTLSNINMLLAFVVLIGLFIVGPTLYILNTFTNGLGNYIANFFSMSLRIPTGGQKFQWLQNWTIFYWAW
WISWAPFVGIFIARVSKGRTIKEFILGVLFVPALVCFIFFAVFGASAIYLQDNHIADIAKAATETATFATLQHYPLGFVL
SLITLLVIMIFFVTSADSATYVLGMLSSSGDINPKSFVKVSWGIIMALFAIIMIYTGGTQAIQNLLIIAALPFSVVIIAM
IWSLIKSLSEEKPRNSNKVLIKHRDPDILEYRSSKHIDEN

Specific function: High-affinity uptake of glycine betaine [H]

COG id: COG1292

COG function: function code M; Choline-glycine betaine transporter

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the BCCT transporter (TC 2.A.15) family [H]

Homologues:

Organism=Escherichia coli, GI1786506, Length=520, Percent_Identity=35.7692307692308, Blast_Score=325, Evalue=6e-90,
Organism=Escherichia coli, GI1788102, Length=451, Percent_Identity=32.3725055432373, Blast_Score=229, Evalue=4e-61,
Organism=Escherichia coli, GI1786224, Length=459, Percent_Identity=29.1938997821351, Blast_Score=193, Evalue=3e-50,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000060
- InterPro:   IPR018093 [H]

Pfam domain/function: PF02028 BCCT [H]

EC number: NA

Molecular weight: Translated: 57637; Mature: 57637

Theoretical pI: Translated: 8.92; Mature: 8.92

Prosite motif: PS01303 BCCT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNKSVSLVFWVSLVICTIFVAFGAIFPKQLEKLTQNITTFIALHFSWYYLLLVLVILFV
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
CVYILFSRYANITLGEEGEDPEFSLPSWFAMLFSAGMGIGLVFWTTAEPISHAFKLTPIH
HHHHHHHHCCCCEECCCCCCCCCCCHHHHHHHHHCCCCCEEEEEECCHHHHHHEEECCCH
KAGTQSAINDAMQFSFFHWGIHAWAVYGIVALVFAYFSFHKGYPGLVSATLTPLLGEKAM
HCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
RGPLGGAIDVLAVIATVTGVAATLGFGALQINEGLHFLFNVPSNFTMQVILIVIATILFT
CCCCCHHHHHHHHHHHHHHHHHHHCCCCEEECCCCEEEEECCCCHHHHHHHHHHHHHHHH
WSAWSGIDKGIKTLSNINMLLAFVVLIGLFIVGPTLYILNTFTNGLGNYIANFFSMSLRI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEC
PTGGQKFQWLQNWTIFYWAWWISWAPFVGIFIARVSKGRTIKEFILGVLFVPALVCFIFF
CCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
AVFGASAIYLQDNHIADIAKAATETATFATLQHYPLGFVLSLITLLVIMIFFVTSADSAT
HHHCCCEEEEECCHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHCCCCHH
YVLGMLSSSGDINPKSFVKVSWGIIMALFAIIMIYTGGTQAIQNLLIIAALPFSVVIIAM
HHHEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
IWSLIKSLSEEKPRNSNKVLIKHRDPDILEYRSSKHIDEN
HHHHHHHHHHCCCCCCCEEEEEECCCCHHHHCCCCCCCCC
>Mature Secondary Structure
MKNKSVSLVFWVSLVICTIFVAFGAIFPKQLEKLTQNITTFIALHFSWYYLLLVLVILFV
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
CVYILFSRYANITLGEEGEDPEFSLPSWFAMLFSAGMGIGLVFWTTAEPISHAFKLTPIH
HHHHHHHHCCCCEECCCCCCCCCCCHHHHHHHHHCCCCCEEEEEECCHHHHHHEEECCCH
KAGTQSAINDAMQFSFFHWGIHAWAVYGIVALVFAYFSFHKGYPGLVSATLTPLLGEKAM
HCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
RGPLGGAIDVLAVIATVTGVAATLGFGALQINEGLHFLFNVPSNFTMQVILIVIATILFT
CCCCCHHHHHHHHHHHHHHHHHHHCCCCEEECCCCEEEEECCCCHHHHHHHHHHHHHHHH
WSAWSGIDKGIKTLSNINMLLAFVVLIGLFIVGPTLYILNTFTNGLGNYIANFFSMSLRI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEC
PTGGQKFQWLQNWTIFYWAWWISWAPFVGIFIARVSKGRTIKEFILGVLFVPALVCFIFF
CCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
AVFGASAIYLQDNHIADIAKAATETATFATLQHYPLGFVLSLITLLVIMIFFVTSADSAT
HHHCCCEEEEECCHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHCCCCHH
YVLGMLSSSGDINPKSFVKVSWGIIMALFAIIMIYTGGTQAIQNLLIIAALPFSVVIIAM
HHHEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
IWSLIKSLSEEKPRNSNKVLIKHRDPDILEYRSSKHIDEN
HHHHHHHHHHCCCCCCCEEEEEECCCCHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; choline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + choline [Periplasm] = Proton [Cytoplasm] + choline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8752321; 9387221; 9384377 [H]